Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
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YGR198W | 8.67 | 2.13E-18 | 2.46 | YPP1 | Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene |
YDR211W | 6.22 | 2.55E-10 | 0.49 | GCD6 | Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress |
YOL120C | 5.73 | 5.09E-9 | 0.51 | RPL18A | Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication |
YGR098C | 5.22 | 9.06E-8 | 1.09 | ESP1 | Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress |
YNR017W | 4.13 | 1.81E-5 | 0.01 | TIM23 | Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel |
YDR404C | 4.12 | 1.89E-5 | 0.04 | RPB7 | RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation |
YGL225W | 4.08 | 2.29E-5 | 0.53 | VRG4 | Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi |
YDR288W | 3.54 | 1.98E-4 | 0.04 | NSE3 | Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress |
YLR117C | 3.51 | 2.27E-4 | 0.03 | CLF1 | Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins |
YGR095C | 3.47 | 2.57E-4 | 0.12 | RRP46 | Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) |
YDR280W | 3.35 | 4.00E-4 | 0.16 | RRP45 | Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress |
YDR355C_d | 3.19 | 7.00E-4 | 0.01 | YDR355C_d | Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W |
YPR113W | 3.19 | 7.18E-4 | 0.07 | PIS1 | Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins |
YML010W | 3.12 | 8.98E-4 | 0.01 | SPT5 | Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair |
YJL061W | 3.11 | 9.35E-4 | 0.13 | NUP82 | Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) |