k081-0041

5-bromo-N-(3-chloro-4-methoxyphenyl)pyridine-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1035
Screen concentration 55.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 731709
SMILES COC1=C(C=C(C=C1)NC(=O)C2=CC(=CN=C2)Br)Cl
Standardized SMILES COc1ccc(NC(=O)c2cncc(Br)c2)cc1Cl
Molecular weight 341.5877
ALogP 2.86
H-bond donor count 1
H-bond acceptor count 3
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.21
% growth inhibition (Hom. pool) 4.97


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 731709
Download HIP data (tab-delimited text)  (excel)
Gene:CLF1(YLR117C)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.03||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:ESP1(YGR098C)|FD-Score:5.22|P-value:9.06E-8|Clearance:1.09||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD10(YNL062C)|FD-Score:-3.56|P-value:1.82E-4|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:6.22|P-value:2.55E-10|Clearance:0.49||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:NSE3(YDR288W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.04||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP82(YJL061W)|FD-Score:3.11|P-value:9.35E-4|Clearance:0.13||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PIS1(YPR113W)|FD-Score:3.19|P-value:7.18E-4|Clearance:0.07||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:RPB7(YDR404C)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.04||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL18A(YOL120C)|FD-Score:5.73|P-value:5.09E-9|Clearance:0.51||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRP45(YDR280W)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.16||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.12||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SPT5(YML010W)|FD-Score:3.12|P-value:8.98E-4|Clearance:0.01||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:TIM23(YNR017W)|FD-Score:4.13|P-value:1.81E-5|Clearance:0.01||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:VRG4(YGL225W)|FD-Score:4.08|P-value:2.29E-5|Clearance:0.53||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YDR355C(YDR355C_d)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.01||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:YGR115C(YGR115C_d)|FD-Score:-3.31|P-value:4.65E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YPP1(YGR198W)|FD-Score:8.67|P-value:2.13E-18|Clearance:2.46||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:CLF1(YLR117C)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.03||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:ESP1(YGR098C)|FD-Score:5.22|P-value:9.06E-8|Clearance:1.09||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD10(YNL062C)|FD-Score:-3.56|P-value:1.82E-4|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GCD6(YDR211W)|FD-Score:6.22|P-value:2.55E-10|Clearance:0.49||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:NSE3(YDR288W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.04||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP82(YJL061W)|FD-Score:3.11|P-value:9.35E-4|Clearance:0.13||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PIS1(YPR113W)|FD-Score:3.19|P-value:7.18E-4|Clearance:0.07||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:RPB7(YDR404C)|FD-Score:4.12|P-value:1.89E-5|Clearance:0.04||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL18A(YOL120C)|FD-Score:5.73|P-value:5.09E-9|Clearance:0.51||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRP45(YDR280W)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.16||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.12||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SPT5(YML010W)|FD-Score:3.12|P-value:8.98E-4|Clearance:0.01||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:TIM23(YNR017W)|FD-Score:4.13|P-value:1.81E-5|Clearance:0.01||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:VRG4(YGL225W)|FD-Score:4.08|P-value:2.29E-5|Clearance:0.53||SGD DESC:Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi Gene:YDR355C(YDR355C_d)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.01||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:YGR115C(YGR115C_d)|FD-Score:-3.31|P-value:4.65E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YPP1(YGR198W)|FD-Score:8.67|P-value:2.13E-18|Clearance:2.46||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 731709
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AFT2(YPL202C)|FD-Score:6.16|P-value:3.67E-10||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ALK1(YGL021W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:ANT1(YPR128C)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:AVT3(YKL146W)|FD-Score:6.09|P-value:5.71E-10||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BDF1(YLR399C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BIM1(YER016W)|FD-Score:-4.33|P-value:7.45E-6||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BOR1(YNL275W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BUB3(YOR026W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:COG8(YML071C)|FD-Score:6.39|P-value:8.05E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTA1(YDR256C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CWH41(YGL027C)|FD-Score:-4.58|P-value:2.34E-6||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DNF2(YDR093W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DOA1(YKL213C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DOT1(YDR440W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DUG3(YNL191W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAR1(YMR171C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:FMP48(YGR052W_p)|FD-Score:5.4|P-value:3.33E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUN30(YAL019W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GET1(YGL020C)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GSF2(YML048W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HFD1(YMR110C)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:HRT3(YLR097C)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:HXT2(YMR011W)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:LDB19(YOR322C)|FD-Score:-4.43|P-value:4.69E-6||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LGE1(YPL055C)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LTE1(YAL024C)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:LYS20(YDL182W)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication Gene:MRM1(YOR201C)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:NDJ1(YOL104C)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NUM1(YDR150W)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:ORM1(YGR038W)|FD-Score:6.22|P-value:2.55E-10||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PIR3(YKL163W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PMS1(YNL082W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRO2(YOR323C)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRY2(YKR013W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PUT2(YHR037W)|FD-Score:-5.28|P-value:6.47E-8||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAD16(YBR114W)|FD-Score:-4.37|P-value:6.13E-6||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RED1(YLR263W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:REG1(YDR028C)|FD-Score:5.47|P-value:2.27E-8||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFM1(YOR279C)|FD-Score:4.84|P-value:6.40E-7||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPE1(YJL121C)|FD-Score:-3.76|P-value:8.54E-5||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL6B(YLR448W)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPP2B(YDR382W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS17B(YDR447C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS22A(YJL190C)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RTT103(YDR289C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAM37(YMR060C)|FD-Score:5.26|P-value:7.13E-8||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SFP1(YLR403W)|FD-Score:5.7|P-value:6.13E-9||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SIP4(YJL089W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SIW14(YNL032W)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SKM1(YOL113W)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SSD1(YDR293C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STV1(YMR054W)|FD-Score:3.74|P-value:9.17E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:UGA3(YDL170W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:URA7(YBL039C)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:VBA2(YBR293W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS72(YDR485C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAL066W(YAL066W_d)|FD-Score:-3.15|P-value:8.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR226C(YBR226C_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YBR232C(YBR232C_d)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR101C(YCR101C_p)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDL144C(YDL144C_p)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR102C(YDR102C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YDR344C(YDR344C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR054W(YGR054W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YGR273C(YGR273C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YMR187C(YMR187C_p)|FD-Score:6.26|P-value:1.94E-10||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YOR378W(YOR378W_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene Gene:YPL034W(YPL034W_p)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPT53(YNL093W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:YRM1(YOR172W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes Gene:ADK1(YDR226W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AFT2(YPL202C)|FD-Score:6.16|P-value:3.67E-10||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ALK1(YGL021W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:ANT1(YPR128C)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:AVT3(YKL146W)|FD-Score:6.09|P-value:5.71E-10||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BDF1(YLR399C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BIM1(YER016W)|FD-Score:-4.33|P-value:7.45E-6||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BOR1(YNL275W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BUB3(YOR026W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Kinetochore checkpoint WD40 repeat protein that localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p Gene:COG8(YML071C)|FD-Score:6.39|P-value:8.05E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTA1(YDR256C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CWH41(YGL027C)|FD-Score:-4.58|P-value:2.34E-6||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DNF2(YDR093W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DOA1(YKL213C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DOT1(YDR440W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DUG3(YNL191W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAR1(YMR171C)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:FMP48(YGR052W_p)|FD-Score:5.4|P-value:3.33E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUN30(YAL019W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:GET1(YGL020C)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GSF2(YML048W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HFD1(YMR110C)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder Gene:HRT3(YLR097C)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:HXT2(YMR011W)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:LDB19(YOR322C)|FD-Score:-4.43|P-value:4.69E-6||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LGE1(YPL055C)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LTE1(YAL024C)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:LYS20(YDL182W)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication Gene:MRM1(YOR201C)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:NDJ1(YOL104C)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NUM1(YDR150W)|FD-Score:-3.3|P-value:4.83E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:ORM1(YGR038W)|FD-Score:6.22|P-value:2.55E-10||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PIR3(YKL163W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PMS1(YNL082W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRO2(YOR323C)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRY2(YKR013W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PUT2(YHR037W)|FD-Score:-5.28|P-value:6.47E-8||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAD16(YBR114W)|FD-Score:-4.37|P-value:6.13E-6||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RED1(YLR263W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:REG1(YDR028C)|FD-Score:5.47|P-value:2.27E-8||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFM1(YOR279C)|FD-Score:4.84|P-value:6.40E-7||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPE1(YJL121C)|FD-Score:-3.76|P-value:8.54E-5||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL6B(YLR448W)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPP2B(YDR382W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS17B(YDR447C)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS22A(YJL190C)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RTT103(YDR289C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAM37(YMR060C)|FD-Score:5.26|P-value:7.13E-8||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SFP1(YLR403W)|FD-Score:5.7|P-value:6.13E-9||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SIP4(YJL089W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SIW14(YNL032W)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SKM1(YOL113W)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SSD1(YDR293C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STV1(YMR054W)|FD-Score:3.74|P-value:9.17E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:UGA3(YDL170W)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:URA7(YBL039C)|FD-Score:-4.05|P-value:2.55E-5||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:VBA2(YBR293W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS72(YDR485C)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAL066W(YAL066W_d)|FD-Score:-3.15|P-value:8.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR226C(YBR226C_d)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YBR232C(YBR232C_d)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR101C(YCR101C_p)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDL144C(YDL144C_p)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR102C(YDR102C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YDR344C(YDR344C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR054W(YGR054W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A Gene:YGR273C(YGR273C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YMR187C(YMR187C_p)|FD-Score:6.26|P-value:1.94E-10||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YOR378W(YOR378W_p)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene Gene:YPL034W(YPL034W_p)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPT53(YNL093W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:YRM1(YOR172W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR198W8.672.13E-182.46YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YDR211W6.222.55E-100.49GCD6Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
YOL120C5.735.09E-90.51RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YGR098C5.229.06E-81.09ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YNR017W4.131.81E-50.01TIM23Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel
YDR404C4.121.89E-50.04RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YGL225W4.082.29E-50.53VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YDR288W3.541.98E-40.04NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YLR117C3.512.27E-40.03CLF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
YGR095C3.472.57E-40.12RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YDR280W3.354.00E-40.16RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YDR355C_d3.197.00E-40.01YDR355C_dDubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
YPR113W3.197.18E-40.07PIS1Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
YML010W3.128.98E-40.01SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YJL061W3.119.35E-40.13NUP82Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML071C6.398.05E-11COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YMR187C_p6.261.94E-10YMR187C_pPutative protein of unknown function; YMR187C is not an essential gene
YGR038W6.222.55E-10ORM1Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control
YPL202C6.163.67E-10AFT2Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
YKL146W6.095.71E-10AVT3Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YLR403W5.706.13E-9SFP1Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
YDR028C5.472.27E-8REG1Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p
YGR052W_p5.403.33E-8FMP48_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
YMR060C5.267.13E-8SAM37Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
YJL190C5.151.30E-7RPS22AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication
YOL104C5.022.61E-7NDJ1Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement
YNL032W4.973.33E-7SIW14Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm
YOR279C4.846.40E-7RFM1DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance
YOL113W4.562.57E-6SKM1Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth
YMR011W4.493.54E-6HXT2High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose

GO enrichment analysis for SGTC_1035
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1411.29E-27SGTC_1039k821-0043 94.0 μMChemDiv (Drug-like library)159927680.226667Golgi
0.0921.29E-12SGTC_3660181-0206 4.3 μMChemDiv (Drug-like library)2748740.134615Golgi
0.0708.57E-8SGTC_1034k072-0165 12.7 μMChemDiv (Drug-like library)58354610.225806Golgi
0.0663.76E-7SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0952381heme requiring
0.0647.11E-7SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.191176Golgi
0.0594.86E-6SGTC_23327982454 138.9 μMChembridge (Fragment library)29817480.111111
0.0589.52E-6SGTC_32339132340 49.5 μMChembridge (Drug-like library)176829500.228571
0.0571.10E-5SGTC_8500388-0143 75.9 μMChemDiv (Drug-like library)672730.174603
0.0571.12E-5SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.125Golgi
0.0571.40E-5SGTC_22737815444 169.1 μMChembridge (Fragment library)28246160.130435
0.0561.51E-5SGTC_488nitrendipine 139.0 μMMiscellaneous45070.128205
0.0561.52E-5SGTC_24855633444 17.8 μMMiscellaneous57242390.157143redox potentiating
0.0561.94E-5SGTC_40960-0234 25.7 μMChemDiv (Drug-like library)68211120.166667Golgi
0.0561.84E-5SGTC_1530rhein 70.4 μMTimTec (Pure natural product library)101680.123077
0.0552.69E-5SGTC_31729105863 49.5 μMChembridge (Drug-like library)23487850.0864198ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1462k081-001426.5 μM0.6808513270634ChemDiv (Drug-like library)329.5521833.08213
SGTC_2943906852945.32 μM0.6808513270634Yeast Active Library329.5521833.08213
SGTC_2941905614511.23 μM0.6078432119517Chembridge (Drug-like library)379.559693.81915
SGTC_1463k081-003234.1 μM0.509434977755ChemDiv (Drug-like library)345.114633.15415
SGTC_3298911685871.43 μM0.5094342506942Chembridge (Drug-like library)279.718862.65713
SGTC_3010908008971.43 μM0.48275917027233Chembridge (Drug-like library)378.2387833.49714
SGTC_2787528454071.43 μM0.45283736958Chembridge (Drug-like library)291.729563.24613
SGTC_3186911046449.47 μM0.44230817018021Chembridge (Drug-like library)269.338264.05612RPP1 & pyrimidine depletion
SGTC_3176910658249.47 μM0.43636417018027Chembridge (Drug-like library)275.730163.74812
SGTC_14524194-0072190 μM0.4307691343545ChemDiv (Drug-like library)408.248064.14914