k284-1091

4-[(4-fluorophenyl)methylamino]-1H-quinazoline-2-thione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1037
Screen concentration 78.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 3334711
SMILES C1=CC=C2C(=C1)C(=NC(=S)N2)NCC3=CC=C(C=C3)F
Standardized SMILES Fc1ccc(CNc2nc(S)nc3ccccc23)cc1
Molecular weight 285.3393
ALogP 3.99
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 22.02
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3334711
Download HIP data (tab-delimited text)  (excel)
Gene:APC1(YNL172W)|FD-Score:-3.42|P-value:3.09E-4|Clearance:0||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:ARC40(YBR234C)|FD-Score:-3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP7(YPR034W)|FD-Score:5.02|P-value:2.58E-7|Clearance:0.91||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC31(YOR257W)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.04||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DED1(YOR204W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.04||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:NOP15(YNL110C)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NUP82(YJL061W)|FD-Score:3.1|P-value:9.77E-4|Clearance:0.08||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:QRI1(YDL103C)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RNA14(YMR061W)|FD-Score:4.52|P-value:3.08E-6|Clearance:0.91||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB7(YDR404C)|FD-Score:3.28|P-value:5.12E-4|Clearance:0.15||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC82(YPR190C)|FD-Score:5.57|P-value:1.29E-8|Clearance:0.91||SGD DESC:RNA polymerase III subunit C82 Gene:RPL18A(YOL120C)|FD-Score:4.46|P-value:4.16E-6|Clearance:0.91||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS20(YHL015W)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.05||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRN3(YKL125W)|FD-Score:3.29|P-value:5.10E-4|Clearance:0||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:RRP4(YHR069C)|FD-Score:3.29|P-value:4.99E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP46(YGR095C)|FD-Score:4.51|P-value:3.27E-6|Clearance:0.91||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP9(YPR137W)|FD-Score:4.32|P-value:7.97E-6|Clearance:0.91||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SMD2(YLR275W)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.02||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TAF7(YMR227C)|FD-Score:4.91|P-value:4.61E-7|Clearance:0.91||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:YPP1(YGR198W)|FD-Score:-4.39|P-value:5.64E-6|Clearance:0||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:APC1(YNL172W)|FD-Score:-3.42|P-value:3.09E-4|Clearance:0||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:ARC40(YBR234C)|FD-Score:-3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP7(YPR034W)|FD-Score:5.02|P-value:2.58E-7|Clearance:0.91||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC31(YOR257W)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.04||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DED1(YOR204W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.04||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:NOP15(YNL110C)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.01||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NUP82(YJL061W)|FD-Score:3.1|P-value:9.77E-4|Clearance:0.08||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:QRI1(YDL103C)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RNA14(YMR061W)|FD-Score:4.52|P-value:3.08E-6|Clearance:0.91||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB7(YDR404C)|FD-Score:3.28|P-value:5.12E-4|Clearance:0.15||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC82(YPR190C)|FD-Score:5.57|P-value:1.29E-8|Clearance:0.91||SGD DESC:RNA polymerase III subunit C82 Gene:RPL18A(YOL120C)|FD-Score:4.46|P-value:4.16E-6|Clearance:0.91||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS20(YHL015W)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.05||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRN3(YKL125W)|FD-Score:3.29|P-value:5.10E-4|Clearance:0||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:RRP4(YHR069C)|FD-Score:3.29|P-value:4.99E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP46(YGR095C)|FD-Score:4.51|P-value:3.27E-6|Clearance:0.91||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP9(YPR137W)|FD-Score:4.32|P-value:7.97E-6|Clearance:0.91||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SMD2(YLR275W)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.02||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TAF7(YMR227C)|FD-Score:4.91|P-value:4.61E-7|Clearance:0.91||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:YPP1(YGR198W)|FD-Score:-4.39|P-value:5.64E-6|Clearance:0||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3334711
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AIM21(YIR003W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:ARG7(YMR062C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ASF2(YDL197C)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Anti-silencing protein that causes derepression of silent loci when overexpressed Gene:ATP5(YDR298C)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:CAF16(YFL028C)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CAT2(YML042W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CAT8(YMR280C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CCC2(YDR270W)|FD-Score:4.3|P-value:8.69E-6||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CDC26(YFR036W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHS3(YBR023C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CTR1(YPR124W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYS3(YAL012W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DER1(YBR201W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p Gene:FIS1(YIL065C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FLO1(YAR050W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FMP21(YBR269C_p)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP48(YGR052W_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUR4(YBR021W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:HNT3(YOR258W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC4(YDR540C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MRPS12(YNR036C)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRS2(YOR334W)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:NAP1(YKR048C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NTO1(YPR031W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PCH2(YBR186W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation Gene:PEP12(YOR036W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:POF1(YCL047C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:POR2(YIL114C)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PTC6(YCR079W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RAM1(YDL090C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RGP1(YDR137W)|FD-Score:4.81|P-value:7.54E-7||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RTK1(YDL025C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SBH1(YER087C-B)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEC66(YBR171W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIP4(YJL089W)|FD-Score:4.43|P-value:4.82E-6||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SPO7(YAL009W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:TIR1(YER011W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TOM1(YDR457W)|FD-Score:-4.02|P-value:2.88E-5||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:VMA16(YHR026W)|FD-Score:5.82|P-value:2.90E-9||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VTC3(YPL019C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:WAR1(YML076C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR087W(YCR087W_d)|FD-Score:5.5|P-value:1.93E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR535C(YDR535C_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEF1(YEL041W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YET3(YDL072C)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGL138C(YGL138C_p)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YHR210C(YHR210C_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIH1(YCR059C)|FD-Score:4.32|P-value:7.78E-6||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJL150W(YJL150W_d)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR053C(YLR053C_p)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function Gene:YLR428C(YLR428C_d)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YNL134C(YNL134C_p)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YNL203C(YNL203C_d)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL073C(YPL073C_d)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL107W(YPL107W_p)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR027C(YPR027C_p)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function Gene:YPR096C(YPR096C)|FD-Score:-3.78|P-value:7.98E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:AAT2(YLR027C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AIM21(YIR003W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:ARG7(YMR062C)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ASF2(YDL197C)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Anti-silencing protein that causes derepression of silent loci when overexpressed Gene:ATP5(YDR298C)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:CAF16(YFL028C)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CAT2(YML042W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CAT8(YMR280C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CCC2(YDR270W)|FD-Score:4.3|P-value:8.69E-6||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CDC26(YFR036W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHS3(YBR023C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CTR1(YPR124W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYS3(YAL012W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DER1(YBR201W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for ER-associated protein degradation of misfolded or unassembled proteins; N- and C- termini protrude into the cytoplasm, has similarity to Dfm1p Gene:FIS1(YIL065C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FLO1(YAR050W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FMP21(YBR269C_p)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP48(YGR052W_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUR4(YBR021W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:HNT3(YOR258W)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC4(YDR540C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MRPS12(YNR036C)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRS2(YOR334W)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:NAP1(YKR048C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NTO1(YPR031W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:PCH2(YBR186W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation Gene:PEP12(YOR036W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:POF1(YCL047C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:POR2(YIL114C)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PTC6(YCR079W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RAM1(YDL090C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RGP1(YDR137W)|FD-Score:4.81|P-value:7.54E-7||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RTK1(YDL025C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SBH1(YER087C-B)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEC66(YBR171W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIP4(YJL089W)|FD-Score:4.43|P-value:4.82E-6||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SPO7(YAL009W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:TIR1(YER011W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TOM1(YDR457W)|FD-Score:-4.02|P-value:2.88E-5||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:VMA16(YHR026W)|FD-Score:5.82|P-value:2.90E-9||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VTC3(YPL019C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:WAR1(YML076C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively Gene:YBR200W-A(YBR200W-A_p)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR087W(YCR087W_d)|FD-Score:5.5|P-value:1.93E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR169C-A(YDR169C-A_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR535C(YDR535C_d)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEF1(YEL041W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YET3(YDL072C)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGL138C(YGL138C_p)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YHR210C(YHR210C_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIH1(YCR059C)|FD-Score:4.32|P-value:7.78E-6||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJL150W(YJL150W_d)|FD-Score:-3.19|P-value:7.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR053C(YLR053C_p)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function Gene:YLR428C(YLR428C_d)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YNL134C(YNL134C_p)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YNL203C(YNL203C_d)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL073C(YPL073C_d)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL107W(YPL107W_p)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR027C(YPR027C_p)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative protein of unknown function Gene:YPR096C(YPR096C)|FD-Score:-3.78|P-value:7.98E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR190C5.571.29E-80.91RPC82RNA polymerase III subunit C82
YPR034W5.022.58E-70.91ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YMR227C4.914.61E-70.91TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YMR061W4.523.08E-60.91RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YGR095C4.513.27E-60.91RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YOL120C4.464.16E-60.91RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YPR137W4.327.97E-60.91RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YNL110C3.413.30E-40.01NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YOR204W3.403.42E-40.04DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YLR275W3.363.92E-40.02SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YHL015W3.344.22E-40.05RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YHR069C3.294.99E-40.01RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YKL125W3.295.10E-40.00RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YDR404C3.285.12E-40.15RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YOR257W3.148.53E-40.04CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR026W5.822.90E-9VMA16Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
YCR087W_d5.501.93E-8YCR087W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene
YMR062C5.141.39E-7ARG7Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
YDR298C5.032.45E-7ATP5Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
YDR137W4.817.54E-7RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YBR269C_p4.562.52E-6FMP21_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YNR036C4.552.73E-6MRPS12Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins
YJL089W4.434.82E-6SIP4C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus
YCR059C4.327.78E-6YIH1Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
YDR270W4.308.69E-6CCC2Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
YBR200W-A_p4.141.75E-5YBR200W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YBR171W4.013.09E-5SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YOR036W4.003.23E-5PEP12Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
YPL107W_p3.983.43E-5YPL107W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene
YOR258W3.846.13E-5HNT3DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress

GO enrichment analysis for SGTC_1037
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0979.54E-14SGTC_30079078383 71.4 μMChembridge (Drug-like library)64969950.0705882
0.0864.15E-11SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.103896translation
0.0774.15E-9SGTC_6911548-0686 185.0 μMChemDiv (Drug-like library)464951330.0909091
0.0715.91E-8SGTC_10523253-3998 198.0 μMChemDiv (Drug-like library)54503170.0731707
0.0708.71E-8SGTC_2526patulin 37.6 μMMicrosource (Natural product library)46960.0322581
0.0708.96E-8SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.0384615RNA processing & uracil transport
0.0691.19E-7SGTC_11544088-0362 150.0 μMChemDiv (Drug-like library)66183040.056338
0.0673.26E-7SGTC_15823',3',6'-trihydroxyflavone 74.0 μMTimTec (Pure natural product library)6888030.0769231
0.0663.98E-7SGTC_21535569715 200.0 μMChembridge (Fragment library)20547010.147541iron homeostasis
0.0664.21E-7SGTC_8790831-1030 162.0 μMChemDiv (Drug-like library)15492520.0689655
0.0664.59E-7SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.016129RSC complex & mRNA processing
0.0656.43E-7SGTC_1571348-1695 65.8 μMChemDiv (Drug-like library)53805100.107692iron homeostasis
0.0648.05E-7SGTC_8380109-0203 71.4 μMChemDiv (Drug-like library)7594220.1
0.0622.03E-6SGTC_7083448-5400 146.0 μMChemDiv (Drug-like library)34621950.0978261
0.0622.37E-6SGTC_23519026858 72.3 μMChembridge (Fragment library)14093000.15RNA processing & uracil transport

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1505k297-003720.6 μM0.7619054089396ChemDiv (Drug-like library)301.793884.44421
SGTC_282k297-003665.7 μM0.6382985061378ChemDiv (Drug-like library)301.793884.44421
SGTC_1467k284-1082293 μM0.5957453334705ChemDiv (Drug-like library)273.376543.5222
SGTC_1468k284-1086134 μM0.52830215996457ChemDiv (Drug-like library)360.453842.80634DNA intercalators
SGTC_1504k297-003330 μM0.406254388117ChemDiv (Drug-like library)413.966764.69123
SGTC_14033909-781562.9 μM0.2923086615483ChemDiv (Drug-like library)389.4222035.11216
SGTC_21485546263113.57 μM0.283019792097Chembridge (Fragment library)216.2541232.76313
SGTC_5694130-2812160 μM0.264151743552ChemDiv (Drug-like library)279.3298633.01614amide catabolism
SGTC_2982901940425.97 μM0.25806522333232Chembridge (Drug-like library)316.3500233.98506
SGTC_3275913806949.47 μM0.25454517617007Chembridge (Drug-like library)293.3564433.30714