k898-0338

2-[4-amino-5-(4-bromophenyl)-3-methylpyrazolo[3,4-d]pyrimidin-6-yl]sulfanyl-N-(furan-2-ylmethyl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1040
Screen concentration 155.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 20969351
SMILES CC1=NN=C2C1=C(N(C(=N2)SCC(=O)NCC3=CC=CO3)C4=CC=C(C=C4)Br)N
Standardized SMILES Cc1nnc2nc(SCC(=O)NCc3occc3)n(c(N)c12)c4ccc(Br)cc4
Molecular weight 473.3463
ALogP 2.73
H-bond donor count 2
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -2.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 20969351
Download HIP data (tab-delimited text)  (excel)
Gene:CAB3(YKL088W)|FD-Score:4.2|P-value:1.32E-5|Clearance:1.18||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:NAT2(YGR147C)|FD-Score:-3.8|P-value:7.30E-5|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:RPN3(YER021W)|FD-Score:-3.21|P-value:6.70E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RRN7(YJL025W)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SCC2(YDR180W)|FD-Score:-4.35|P-value:6.79E-6|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:TOM22(YNL131W)|FD-Score:-4.42|P-value:4.89E-6|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YPP1(YGR198W)|FD-Score:6.21|P-value:2.71E-10|Clearance:2||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:CAB3(YKL088W)|FD-Score:4.2|P-value:1.32E-5|Clearance:1.18||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:NAT2(YGR147C)|FD-Score:-3.8|P-value:7.30E-5|Clearance:0||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:RPN3(YER021W)|FD-Score:-3.21|P-value:6.70E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RRN7(YJL025W)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SCC2(YDR180W)|FD-Score:-4.35|P-value:6.79E-6|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:TOM22(YNL131W)|FD-Score:-4.42|P-value:4.89E-6|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YPP1(YGR198W)|FD-Score:6.21|P-value:2.71E-10|Clearance:2||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 20969351
Download HOP data (tab-delimited text)  (excel)
Gene:AAD4(YDL243C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:APT2(YDR441C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ATF1(YOR377W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:ATG18(YFR021W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:AVT1(YJR001W)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAF120(YNL278W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CAM1(YPL048W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CHS6(YJL099W)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COX11(YPL132W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:CTM1(YHR109W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:DAL80(YKR034W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DOT1(YDR440W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:FAR10(YLR238W)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:GFD1(YMR255W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GFD2(YCL036W)|FD-Score:7|P-value:1.32E-12||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GLG1(YKR058W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GSF2(YML048W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GTT2(YLL060C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HEH2(YDR458C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:IRC21(YMR073C)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISF1(YMR081C)|FD-Score:5.04|P-value:2.32E-7||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:KSP1(YHR082C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:MET1(YKR069W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MPC54(YOR177C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL38(YKL170W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPS12(YNR036C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:NPP1(YCR026C)|FD-Score:6.68|P-value:1.20E-11||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OSM1(YJR051W)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PEP12(YOR036W)|FD-Score:-3.93|P-value:4.23E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PET18(YCR020C)|FD-Score:4.51|P-value:3.18E-6||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PEX32(YBR168W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PUF3(YLL013C)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD23(YEL037C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RAV2(YDR202C)|FD-Score:5.43|P-value:2.84E-8||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RIM1(YCR028C-A)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIP1(YEL024W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RPL23A(YBL087C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:STE24(YJR117W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:TDA2(YER071C_p)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TIF4631(YGR162W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TOM1(YDR457W)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOS6(YNL300W_p)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRM732(YMR259C_p)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TVP38(YKR088C)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UGA2(YBR006W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:UGA3(YDL170W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:URA1(YKL216W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VEL1(YGL258W)|FD-Score:-4.58|P-value:2.33E-6||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS15(YBR097W)|FD-Score:-3.78|P-value:7.71E-5||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS21(YOR089C)|FD-Score:4.52|P-value:3.02E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS55(YJR044C)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL071C(YBL071C_d)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL144C(YDL144C_p)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR269C(YDR269C_d)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER135C(YER135C_d)|FD-Score:5.66|P-value:7.55E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YER186C(YER186C_p)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Putative protein of unknown function Gene:YGL159W(YGL159W_p)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR069W(YGR069W_d)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR149W(YGR149W_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YJL027C(YJL027C_p)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Putative protein of unknown function Gene:YJL150W(YJL150W_d)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL097C(YKL097C_d)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YLR279W(YLR279W_d)|FD-Score:5.43|P-value:2.82E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML002W(YML002W_p)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YPL108W(YPL108W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPR003C(YPR003C_p)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:AAD4(YDL243C)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:APT2(YDR441C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication Gene:ATF1(YOR377W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:ATG18(YFR021W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:AVT1(YJR001W)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAF120(YNL278W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CAM1(YPL048W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CHS6(YJL099W)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COX11(YPL132W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:CTM1(YHR109W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:DAL80(YKR034W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DOT1(YDR440W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:FAR10(YLR238W)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:GFD1(YMR255W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GFD2(YCL036W)|FD-Score:7|P-value:1.32E-12||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GLG1(YKR058W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GSF2(YML048W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GTT2(YLL060C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress Gene:HEH2(YDR458C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:IRC21(YMR073C)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:ISF1(YMR081C)|FD-Score:5.04|P-value:2.32E-7||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:KSP1(YHR082C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:MET1(YKR069W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MPC54(YOR177C)|FD-Score:5.56|P-value:1.33E-8||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL38(YKL170W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPS12(YNR036C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:NPP1(YCR026C)|FD-Score:6.68|P-value:1.20E-11||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OSM1(YJR051W)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PEP12(YOR036W)|FD-Score:-3.93|P-value:4.23E-5||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PET18(YCR020C)|FD-Score:4.51|P-value:3.18E-6||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PEX32(YBR168W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PUF3(YLL013C)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD23(YEL037C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RAV2(YDR202C)|FD-Score:5.43|P-value:2.84E-8||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RIM1(YCR028C-A)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIP1(YEL024W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RPL23A(YBL087C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:STE24(YJR117W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:TDA2(YER071C_p)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TIF4631(YGR162W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TOM1(YDR457W)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOS6(YNL300W_p)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TRM732(YMR259C_p)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TVP38(YKR088C)|FD-Score:4.02|P-value:2.97E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UGA2(YBR006W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:UGA3(YDL170W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:URA1(YKL216W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VEL1(YGL258W)|FD-Score:-4.58|P-value:2.33E-6||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS15(YBR097W)|FD-Score:-3.78|P-value:7.71E-5||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:VPS21(YOR089C)|FD-Score:4.52|P-value:3.02E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS55(YJR044C)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL071C(YBL071C_d)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4.48|P-value:3.72E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL144C(YDL144C_p)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR269C(YDR269C_d)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER135C(YER135C_d)|FD-Score:5.66|P-value:7.55E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YER186C(YER186C_p)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Putative protein of unknown function Gene:YGL159W(YGL159W_p)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR069W(YGR069W_d)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR149W(YGR149W_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YJL027C(YJL027C_p)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Putative protein of unknown function Gene:YJL150W(YJL150W_d)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL097C(YKL097C_d)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YLR279W(YLR279W_d)|FD-Score:5.43|P-value:2.82E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML002W(YML002W_p)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YPL108W(YPL108W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPR003C(YPR003C_p)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR198W6.212.71E-102.00YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YKL088W4.201.32E-51.18CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC
YHR020W3.020.001270.05YHR020WProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
YBL026W2.970.001500.03LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YDR189W2.940.001640.00SLY1Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
YLR310C2.940.001650.01CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YLR457C2.920.001720.09NBP1Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
YLR243W_p2.830.002330.05GPN3_pPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
YKL042W2.780.002740.02SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YIL104C2.750.002960.04SHQ1Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
YIR010W2.720.003300.05DSN1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation
YHR070W2.670.003840.06TRM5tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya
YJL156C2.600.004600.00SSY5Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p
YKL104C2.600.004660.03GFA1Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
YHR166C2.570.005060.01CDC23Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCL036W7.001.32E-12GFD2Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication
YCR026C6.681.20E-11NPP1Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication
YER135C_d5.667.55E-9YER135C_dDubious open reading frame unlikely to encode a protein; YER135C is not an essential gene
YOR177C5.561.33E-8MPC54Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate
YLR279W_d5.432.82E-8YLR279W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR202C5.432.84E-8RAV2Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
YNL300W_p5.141.36E-7TOS6_pGlycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid
YGL159W_p5.062.12E-7YGL159W_pPutative protein of unknown function; deletion mutant has no detectable phenotype
YMR081C5.042.32E-7ISF1Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant
YDR457W4.681.44E-6TOM1E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
YOR089C4.523.02E-6VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YJR044C4.523.10E-6VPS55Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP)
YCR020C4.513.18E-6PET18Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
YLR238W4.513.26E-6FAR10Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate
YBR196C-A_p4.483.72E-6YBR196C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR

GO enrichment analysis for SGTC_1040
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0741.23E-8SGTC_6414092-0595 30.0 μMChemDiv (Drug-like library)7862600.0930233
0.0631.13E-6SGTC_32989116858 71.4 μMChembridge (Drug-like library)25069420.10989
0.0631.20E-6SGTC_30539095903 49.5 μMChembridge (Drug-like library)171740180.11828Golgi
0.0631.33E-6SGTC_30979117246 49.5 μMChembridge (Drug-like library)164238920.116505
0.0622.03E-6SGTC_1761st044296 37.0 μMTimTec (Natural product derivative library)18267000.148148
0.0543.21E-5SGTC_1534077-0274 519.7 μMChemDiv (Drug-like library)31219760.0654206
0.0526.46E-5SGTC_1035k081-0041 55.2 μMChemDiv (Drug-like library)7317090.131868Golgi
0.0527.63E-5SGTC_1039k821-0043 94.0 μMChemDiv (Drug-like library)159927680.141414Golgi
0.0501.12E-4SGTC_2569tetrandrine 100.0 μMICCB bioactive library730780.0530973
0.0501.22E-4SGTC_13752910-0797 186.0 μMChemDiv (Drug-like library)7425080.108696
0.0491.55E-4SGTC_3823229-0966 23.2 μMChemDiv (Drug-like library)57223700.0631579
0.0473.56E-4SGTC_11991638-0010 4.5 μMChemDiv (Drug-like library)31111360.0659341endomembrane recycling
0.0463.62E-4SGTC_2641levomentholum 100.0 μMMicrosource (Natural product library)166660.0121951
0.0463.64E-4SGTC_2674ligustilide 100.0 μMMicrosource (Natural product library)53190220.0666667
0.0455.18E-4SGTC_1571348-1695 65.8 μMChemDiv (Drug-like library)53805100.0659341iron homeostasis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9322857-0803154 μM0.382353674898ChemDiv (Drug-like library)280.11732.55112
SGTC_2849901932112.99 μM0.30769216489627Chembridge (Drug-like library)275.365983.21713
SGTC_22737815444169.05 μM0.2857142824616Chembridge (Fragment library)228.268182.00424
SGTC_23327982454138.89 μM0.2784812981748Chembridge (Fragment library)247.316123.15223
SGTC_3297911750271.43 μM0.2716057453720Chembridge (Drug-like library)355.210341.93624
SGTC_7754577-058057.3 μM0.26252006902ChemDiv (Drug-like library)376.289162.86815
SGTC_9463434-133487.4 μM0.26254047976ChemDiv (Drug-like library)266.33932.6324
SGTC_22717849410118.62 μM0.25641972430Chembridge (Fragment library)233.289542.66623
SGTC_21836420625130.51 μM0.2469142897619Chembridge (Fragment library)277.299043.17124mitochondrial processes
SGTC_2846900864219.48 μM0.243592992898Chembridge (Drug-like library)338.219583.51423