0350-0096

1,5-dihydroxy-1,5-di(quinolin-2-yl)pentan-3-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1042
Screen concentration 82.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3122515
SMILES C1=CC=C2C(=C1)C=CC(=N2)C(CC(=O)CC(C3=NC4=CC=CC=C4C=C3)O)O
Standardized SMILES OC(CC(=O)CC(O)c1ccc2ccccc2n1)c3ccc4ccccc4n3
Molecular weight 372.4165
ALogP 3.29
H-bond donor count 2
H-bond acceptor count 5
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.05
% growth inhibition (Hom. pool) 4.68


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3122515
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:5.54|P-value:1.51E-8|Clearance:0.85||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:BRN1(YBL097W)|FD-Score:5.74|P-value:4.86E-9|Clearance:0.85||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CAB1(YDR531W)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CCT5(YJR064W)|FD-Score:6.05|P-value:7.11E-10|Clearance:0.85||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC25(YLR310C)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.05||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC60(YPL160W)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:HYM1(YKL189W)|FD-Score:-3.66|P-value:1.28E-4|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:MVD1(YNR043W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NTF2(YER009W)|FD-Score:4.63|P-value:1.81E-6|Clearance:0.29||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:PDI1(YCL043C)|FD-Score:3.16|P-value:7.84E-4|Clearance:0.05||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PIS1(YPR113W)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP1(YNL221C)|FD-Score:4.69|P-value:1.37E-6|Clearance:0.06||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RPC40(YPR110C)|FD-Score:4.34|P-value:7.18E-6|Clearance:0.07||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPP1(YHR062C)|FD-Score:3.11|P-value:9.33E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC5(YDR166C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.08||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SPT5(YML010W)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:SRP68(YPL243W)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:TCP1(YDR212W)|FD-Score:4.05|P-value:2.55E-5|Clearance:0.61||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:UGP1(YKL035W)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:UTP14(YML093W)|FD-Score:4.26|P-value:1.00E-5|Clearance:0.21||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP23(YOR004W)|FD-Score:-3.2|P-value:6.98E-4|Clearance:0||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein Gene:VTI1(YMR197C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.06||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:WRS1(YOL097C)|FD-Score:3.3|P-value:4.81E-4|Clearance:0.09||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YOR146W(YOR146W_d)|FD-Score:-3.16|P-value:7.99E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:ASA1(YPR085C)|FD-Score:5.54|P-value:1.51E-8|Clearance:0.85||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:BRN1(YBL097W)|FD-Score:5.74|P-value:4.86E-9|Clearance:0.85||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CAB1(YDR531W)|FD-Score:-3.17|P-value:7.59E-4|Clearance:0||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CCT5(YJR064W)|FD-Score:6.05|P-value:7.11E-10|Clearance:0.85||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC25(YLR310C)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.05||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC60(YPL160W)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:HYM1(YKL189W)|FD-Score:-3.66|P-value:1.28E-4|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:MVD1(YNR043W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NTF2(YER009W)|FD-Score:4.63|P-value:1.81E-6|Clearance:0.29||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:PDI1(YCL043C)|FD-Score:3.16|P-value:7.84E-4|Clearance:0.05||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PIS1(YPR113W)|FD-Score:-3.54|P-value:2.02E-4|Clearance:0||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP1(YNL221C)|FD-Score:4.69|P-value:1.37E-6|Clearance:0.06||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RPC40(YPR110C)|FD-Score:4.34|P-value:7.18E-6|Clearance:0.07||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPP1(YHR062C)|FD-Score:3.11|P-value:9.33E-4|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC5(YDR166C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.08||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SPT5(YML010W)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:SRP68(YPL243W)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:TCP1(YDR212W)|FD-Score:4.05|P-value:2.55E-5|Clearance:0.61||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:UGP1(YKL035W)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:UTP14(YML093W)|FD-Score:4.26|P-value:1.00E-5|Clearance:0.21||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP23(YOR004W)|FD-Score:-3.2|P-value:6.98E-4|Clearance:0||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein Gene:VTI1(YMR197C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.06||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:WRS1(YOL097C)|FD-Score:3.3|P-value:4.81E-4|Clearance:0.09||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YOR146W(YOR146W_d)|FD-Score:-3.16|P-value:7.99E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3122515
Download HOP data (tab-delimited text)  (excel)
Gene:ADR1(YDR216W)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ATG17(YLR423C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:AVT2(YEL064C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAM1(YPL048W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CLU1(YMR012W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:CWH41(YGL027C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:CYC7(YEL039C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAL3(YIR032C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIT1(YDR403W)|FD-Score:-3.74|P-value:9.18E-5||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ELF1(YKL160W)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:FLO1(YAR050W)|FD-Score:5.5|P-value:1.95E-8||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:GDH2(YDL215C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GOS1(YHL031C)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GTO3(YMR251W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:GUP1(YGL084C)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HGH1(YGR187C)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HRQ1(YDR291W_p)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:HSE1(YHL002W)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IRC5(YFR038W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:LYS9(YNR050C)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MPP6(YNR024W)|FD-Score:-3.92|P-value:4.49E-5||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MSH3(YCR092C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTC3(YGL226W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NPP1(YCR026C)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OXR1(YPL196W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PEP12(YOR036W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PFK1(YGR240C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIR3(YKL163W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:QCR7(YDR529C)|FD-Score:6.47|P-value:4.91E-11||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RAD5(YLR032W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD61(YDR014W)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RIM21(YNL294C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL23A(YBL087C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:8.2|P-value:1.19E-16||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT2(YBR246W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RSA3(YLR221C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SAP4(YGL229C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SHR5(YOL110W)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SNU66(YOR308C)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:STE24(YJR117W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUT1(YGL162W)|FD-Score:4.3|P-value:8.35E-6||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TIF2(YJL138C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:5.32|P-value:5.09E-8||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMA7(YLR262C-A)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRM732(YMR259C_p)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRP1(YDR007W)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7.31|P-value:1.38E-13||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:26.4|P-value:1.45E-153||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX4(YMR067C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VPS21(YOR089C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR064W(YBR064W_d)|FD-Score:6.31|P-value:1.43E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFL019C(YFL019C_d)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YIL032C(YIL032C_d)|FD-Score:-3.97|P-value:3.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL092W(YIL092W_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YIM1(YMR152W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YJL045W(YJL045W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YJL120W(YJL120W_d)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YLL007C(YLL007C_p)|FD-Score:4.35|P-value:6.89E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YML084W(YML084W_d)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:6.19|P-value:2.99E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR010W(YMR010W_p)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR196W(YMR196W_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL050C(YNL050C_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOL131W(YOL131W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function Gene:YOR053W(YOR053W_d)|FD-Score:4.48|P-value:3.76E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPR096C(YPR096C)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:ADR1(YDR216W)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ATG17(YLR423C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:AVT2(YEL064C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CAM1(YPL048W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CLU1(YMR012W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:CWH41(YGL027C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:CYC7(YEL039C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAL3(YIR032C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIT1(YDR403W)|FD-Score:-3.74|P-value:9.18E-5||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ELF1(YKL160W)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:FLO1(YAR050W)|FD-Score:5.5|P-value:1.95E-8||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:GDH2(YDL215C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GOS1(YHL031C)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GTO3(YMR251W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:GUP1(YGL084C)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HGH1(YGR187C)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:HRQ1(YDR291W_p)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:HSE1(YHL002W)|FD-Score:4.51|P-value:3.24E-6||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IRC5(YFR038W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC1(YER019W)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:LYS9(YNR050C)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MPP6(YNR024W)|FD-Score:-3.92|P-value:4.49E-5||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MSH3(YCR092C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTC3(YGL226W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NPP1(YCR026C)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OXR1(YPL196W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PEP12(YOR036W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PFK1(YGR240C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PIR3(YKL163W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:QCR7(YDR529C)|FD-Score:6.47|P-value:4.91E-11||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RAD5(YLR032W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD61(YDR014W)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RIM21(YNL294C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPL23A(YBL087C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:8.2|P-value:1.19E-16||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT2(YBR246W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RSA3(YLR221C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SAP4(YGL229C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SHR5(YOL110W)|FD-Score:3.85|P-value:5.91E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SNU66(YOR308C)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:STE24(YJR117W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUT1(YGL162W)|FD-Score:4.3|P-value:8.35E-6||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TIF2(YJL138C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:5.32|P-value:5.09E-8||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMA7(YLR262C-A)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRM732(YMR259C_p)|FD-Score:4.34|P-value:7.19E-6||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRP1(YDR007W)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7.31|P-value:1.38E-13||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:26.4|P-value:1.45E-153||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX4(YMR067C)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VPS21(YOR089C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR064W(YBR064W_d)|FD-Score:6.31|P-value:1.43E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFL019C(YFL019C_d)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YIL032C(YIL032C_d)|FD-Score:-3.97|P-value:3.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL092W(YIL092W_p)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YIM1(YMR152W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YJL045W(YJL045W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YJL120W(YJL120W_d)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YLL007C(YLL007C_p)|FD-Score:4.35|P-value:6.89E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YML084W(YML084W_d)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:6.19|P-value:2.99E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR010W(YMR010W_p)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR196W(YMR196W_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL050C(YNL050C_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YOL131W(YOL131W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function Gene:YOR053W(YOR053W_d)|FD-Score:4.48|P-value:3.76E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPR096C(YPR096C)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJR064W6.057.11E-100.85CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YBL097W5.744.86E-90.85BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YPR085C5.541.51E-80.85ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YNL221C4.691.37E-60.06POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YER009W4.631.81E-60.29NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YPR110C4.347.18E-60.07RPC40RNA polymerase subunit AC40, common to RNA polymerase I and III
YML093W4.261.00E-50.21UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YDR212W4.052.55E-50.61TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YDR166C3.442.89E-40.08SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YMR197C3.363.92E-40.06VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YOL097C3.304.81E-40.09WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YLR310C3.216.64E-40.05CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YCL043C3.167.84E-40.05PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YHR062C3.119.33E-40.03RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR207C3.080.001020.17RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A26.401.45E-153TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDL020C8.201.19E-16RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YER090W7.311.38E-13TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR529C6.474.91E-11QCR7Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly
YBR064W_d6.311.43E-10YBR064W_dDubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C
YML122C_d6.192.99E-10YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YAR050W5.501.95E-8FLO1Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p
YPR074C5.325.09E-8TKL1Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
YKL160W4.731.11E-6ELF1Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
YDR354W4.582.29E-6TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YPR096C4.523.16E-6YPR096CProtein of unknown function that may interact with ribosomes, based on co-purification experiments
YHL002W4.513.24E-6HSE1Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
YOR053W_d4.483.76E-6YOR053W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C
YOR308C4.483.77E-6SNU66Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1
YER019W4.454.31E-6ISC1Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance

GO enrichment analysis for SGTC_1042
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5200SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.103448TSC3-RPN4
0.5000SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.185185TSC3-RPN4
0.4830SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.112676TSC3-RPN4
0.4820SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0333333TSC3-RPN4
0.4498.80E-291SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0746269TSC3-RPN4
0.4301.79E-264SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.155172TSC3-RPN4
0.4231.98E-255SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.1
0.4202.76E-251SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0769231TSC3-RPN4
0.4091.86E-237SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.111111TSC3-RPN4
0.4041.71E-230SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.160714TSC3-RPN4
0.3987.67E-223SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.193548TSC3-RPN4
0.3823.60E-204SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0877193TSC3-RPN4
0.3782.61E-200SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0689655TSC3-RPN4
0.3761.51E-197SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.137931
0.3752.18E-196SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.05TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2151556326785.42 μM0.3863645310824Chembridge (Fragment library)199.248422.68512
SGTC_7070865-0008204 μM0.311111723043ChemDiv (Drug-like library)246.26343.89503DNA damage response
SGTC_1705st02786856.33 μM0.311111292948TimTec (Natural product derivative library)256.301284.14902
SGTC_11891431-21197.28 μM0.3043483107275ChemDiv (Drug-like library)254.714244.76912
SGTC_3119912518349.47 μM0.322695334Chembridge (Drug-like library)291.370224.38913
SGTC_20305175110200 μM0.2765962831986Chembridge (Fragment library)188.182721.20523Golgi
SGTC_11383761-00061.03 μM0.26923112061087ChemDiv (Drug-like library)302.392864.69103
SGTC_415dibenzyl ketone100 μM0.2631587593Miscellaneous210.271063.29401
SGTC_18233-hydroxy-2-naphthoic acid106 μM0.2558147104TimTec (Natural product derivative library)188.179422.12623
SGTC_23027771753200 μM0.2553192203047Chembridge (Fragment library)217.286842.13412