1222-0021

5-(4-butylcyclohexyl)pyridine-2-carboxylic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1044
Screen concentration 1.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 4306703
SMILES CCCCC1CCC(CC1)C2=CN=C(C=C2)C(=O)O
Standardized SMILES CCCCC1CCC(CC1)c2ccc(nc2)C(=O)O
Molecular weight 261.3593
ALogP 4.55
H-bond donor count 1
H-bond acceptor count 3
Response signature heme biosynthesis & mitochondrial translocase

Pool Growth Kinetics
% growth inhibition (Het. pool) 40.96
% growth inhibition (Hom. pool) 14.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4306703
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP4(YJL081C)|FD-Score:3.17|P-value:7.62E-4|Clearance:0.02||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:7.61|P-value:1.37E-14|Clearance:1||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET4(YJL031C)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.09||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:-7.39|P-value:7.31E-14|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC36(YDL165W)|FD-Score:-3.1|P-value:9.71E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC39(YCR093W)|FD-Score:-4.25|P-value:1.08E-5|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CMD1(YBR109C)|FD-Score:4.89|P-value:5.01E-7|Clearance:0.09||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:COP1(YDL145C)|FD-Score:3.34|P-value:4.19E-4|Clearance:0.15||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DAD1(YDR016C)|FD-Score:4.74|P-value:1.07E-6|Clearance:0.03||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBP2(YNL112W)|FD-Score:5.38|P-value:3.68E-8|Clearance:0.13||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DML1(YMR211W)|FD-Score:5.45|P-value:2.51E-8|Clearance:0.07||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:EMG1(YLR186W)|FD-Score:-6.58|P-value:2.42E-11|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:ERG11(YHR007C)|FD-Score:-4.35|P-value:6.90E-6|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG12(YMR208W)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.07||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG9(YHR190W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:ESP1(YGR098C)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FAS1(YKL182W)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.12||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GCD11(YER025W)|FD-Score:-3.76|P-value:8.33E-5|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD6(YDR211W)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GLE1(YDL207W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.11||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:HEM1(YDR232W)|FD-Score:9.78|P-value:6.83E-23|Clearance:1.98||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:HEM12(YDR047W)|FD-Score:6.61|P-value:1.88E-11|Clearance:0.35||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM15(YOR176W)|FD-Score:4.11|P-value:1.95E-5|Clearance:0.07||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HEM2(YGL040C)|FD-Score:6.03|P-value:8.41E-10|Clearance:0.24||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HEM3(YDL205C)|FD-Score:15.5|P-value:9.12E-55|Clearance:3.43||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HSH49(YOR319W)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HTB1(YDR224C)|FD-Score:11.6|P-value:2.18E-31|Clearance:0.62||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:HYP2(YEL034W)|FD-Score:6.26|P-value:1.91E-10|Clearance:0.24||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IRA1(YBR140C)|FD-Score:-4.7|P-value:1.28E-6|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MCM1(YMR043W)|FD-Score:7.73|P-value:5.57E-15|Clearance:0.12||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MIA40(YKL195W)|FD-Score:5.15|P-value:1.29E-7|Clearance:0.09||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NFS1(YCL017C)|FD-Score:4.71|P-value:1.26E-6|Clearance:0.37||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NMD3(YHR170W)|FD-Score:-3.26|P-value:5.48E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:-4.08|P-value:2.24E-5|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP1(YDL014W)|FD-Score:-3.96|P-value:3.77E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NUP192(YJL039C)|FD-Score:6.27|P-value:1.86E-10|Clearance:0||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PKC1(YBL105C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.09||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PUP1(YOR157C)|FD-Score:-4.14|P-value:1.72E-5|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RIM2(YBR192W)|FD-Score:5.67|P-value:7.17E-9|Clearance:0.22||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPA43(YOR340C)|FD-Score:-4.07|P-value:2.37E-5|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:-4.35|P-value:6.69E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL18A(YOL120C)|FD-Score:-6.14|P-value:4.05E-10|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL25(YOL127W)|FD-Score:-5.02|P-value:2.63E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPM2(YML091C)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.01||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPN3(YER021W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.01||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN8(YOR261C)|FD-Score:3.15|P-value:8.17E-4|Clearance:0.05||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPN9(YDR427W)|FD-Score:5.24|P-value:8.18E-8|Clearance:0.02||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPS15(YOL040C)|FD-Score:-3.72|P-value:9.98E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS20(YHL015W)|FD-Score:-3.6|P-value:1.61E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPT6(YGL048C)|FD-Score:4.78|P-value:8.68E-7|Clearance:0.04||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:-4.3|P-value:8.67E-6|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP5(YMR229C)|FD-Score:3.98|P-value:3.47E-5|Clearance:0.31||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:RSC4(YKR008W)|FD-Score:11|P-value:2.51E-28|Clearance:1.19||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC58(YLR033W)|FD-Score:7.8|P-value:3.06E-15|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:5.77|P-value:4.00E-9|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.8|P-value:7.86E-7|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSP5(YER125W)|FD-Score:5.78|P-value:3.66E-9|Clearance:0.02||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:RVB2(YPL235W)|FD-Score:-4.73|P-value:1.15E-6|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SFH1(YLR321C)|FD-Score:5.21|P-value:9.22E-8|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SMD3(YLR147C)|FD-Score:-3.2|P-value:6.77E-4|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC105(YGL093W)|FD-Score:5.06|P-value:2.09E-7|Clearance:0.13||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSS1(YDR086C)|FD-Score:12.1|P-value:4.55E-34|Clearance:0.52||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:STH1(YIL126W)|FD-Score:5.26|P-value:7.37E-8|Clearance:0.02||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUI1(YNL244C)|FD-Score:4.71|P-value:1.25E-6|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TFA1(YKL028W)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.01||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF34(YMR146C)|FD-Score:-4.64|P-value:1.77E-6|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIF35(YDR429C)|FD-Score:-3.76|P-value:8.56E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM22(YNL131W)|FD-Score:3.19|P-value:7.02E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:UTP23(YOR004W)|FD-Score:-3.15|P-value:8.03E-4|Clearance:0||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein Gene:UTP4(YDR324C)|FD-Score:-3.89|P-value:5.11E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YBR190W(YBR190W_d)|FD-Score:-4.01|P-value:3.09E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:4.3|P-value:8.63E-6|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR115C(YGR115C_d)|FD-Score:4.93|P-value:4.18E-7|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL195C(YJL195C_d)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YOR146W(YOR146W_d)|FD-Score:-4.38|P-value:5.92E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL142C(YPL142C_d)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YPL238C(YPL238C_d)|FD-Score:-4.01|P-value:3.00E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:4.33|P-value:7.39E-6|Clearance:0.03||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACT1(YFL039C)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP4(YJL081C)|FD-Score:3.17|P-value:7.62E-4|Clearance:0.02||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:7.61|P-value:1.37E-14|Clearance:1||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.01||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET4(YJL031C)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.09||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:-7.39|P-value:7.31E-14|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC36(YDL165W)|FD-Score:-3.1|P-value:9.71E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC39(YCR093W)|FD-Score:-4.25|P-value:1.08E-5|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CMD1(YBR109C)|FD-Score:4.89|P-value:5.01E-7|Clearance:0.09||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:COP1(YDL145C)|FD-Score:3.34|P-value:4.19E-4|Clearance:0.15||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DAD1(YDR016C)|FD-Score:4.74|P-value:1.07E-6|Clearance:0.03||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBP2(YNL112W)|FD-Score:5.38|P-value:3.68E-8|Clearance:0.13||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DML1(YMR211W)|FD-Score:5.45|P-value:2.51E-8|Clearance:0.07||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:EMG1(YLR186W)|FD-Score:-6.58|P-value:2.42E-11|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:ERG11(YHR007C)|FD-Score:-4.35|P-value:6.90E-6|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG12(YMR208W)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.07||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG9(YHR190W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:ESP1(YGR098C)|FD-Score:-3.35|P-value:3.98E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FAS1(YKL182W)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.12||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GCD11(YER025W)|FD-Score:-3.76|P-value:8.33E-5|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD6(YDR211W)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GLE1(YDL207W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.11||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:HEM1(YDR232W)|FD-Score:9.78|P-value:6.83E-23|Clearance:1.98||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:HEM12(YDR047W)|FD-Score:6.61|P-value:1.88E-11|Clearance:0.35||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM15(YOR176W)|FD-Score:4.11|P-value:1.95E-5|Clearance:0.07||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HEM2(YGL040C)|FD-Score:6.03|P-value:8.41E-10|Clearance:0.24||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HEM3(YDL205C)|FD-Score:15.5|P-value:9.12E-55|Clearance:3.43||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HSH49(YOR319W)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HTB1(YDR224C)|FD-Score:11.6|P-value:2.18E-31|Clearance:0.62||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:HYP2(YEL034W)|FD-Score:6.26|P-value:1.91E-10|Clearance:0.24||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IRA1(YBR140C)|FD-Score:-4.7|P-value:1.28E-6|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MCM1(YMR043W)|FD-Score:7.73|P-value:5.57E-15|Clearance:0.12||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:MIA40(YKL195W)|FD-Score:5.15|P-value:1.29E-7|Clearance:0.09||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NFS1(YCL017C)|FD-Score:4.71|P-value:1.26E-6|Clearance:0.37||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:NMD3(YHR170W)|FD-Score:-3.26|P-value:5.48E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC2(YOR206W)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:-4.08|P-value:2.24E-5|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP1(YDL014W)|FD-Score:-3.96|P-value:3.77E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NUP192(YJL039C)|FD-Score:6.27|P-value:1.86E-10|Clearance:0||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PKC1(YBL105C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.09||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PUP1(YOR157C)|FD-Score:-4.14|P-value:1.72E-5|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RIM2(YBR192W)|FD-Score:5.67|P-value:7.17E-9|Clearance:0.22||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPA43(YOR340C)|FD-Score:-4.07|P-value:2.37E-5|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:-4.35|P-value:6.69E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL18A(YOL120C)|FD-Score:-6.14|P-value:4.05E-10|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPL25(YOL127W)|FD-Score:-5.02|P-value:2.63E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPM2(YML091C)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.01||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPN3(YER021W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:3.35|P-value:4.04E-4|Clearance:0.01||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN8(YOR261C)|FD-Score:3.15|P-value:8.17E-4|Clearance:0.05||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPN9(YDR427W)|FD-Score:5.24|P-value:8.18E-8|Clearance:0.02||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:RPS15(YOL040C)|FD-Score:-3.72|P-value:9.98E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS20(YHL015W)|FD-Score:-3.6|P-value:1.61E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPT6(YGL048C)|FD-Score:4.78|P-value:8.68E-7|Clearance:0.04||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:-4.3|P-value:8.67E-6|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP5(YMR229C)|FD-Score:3.98|P-value:3.47E-5|Clearance:0.31||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:RSC4(YKR008W)|FD-Score:11|P-value:2.51E-28|Clearance:1.19||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC58(YLR033W)|FD-Score:7.8|P-value:3.06E-15|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:5.77|P-value:4.00E-9|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.8|P-value:7.86E-7|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSP5(YER125W)|FD-Score:5.78|P-value:3.66E-9|Clearance:0.02||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:RVB2(YPL235W)|FD-Score:-4.73|P-value:1.15E-6|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SFH1(YLR321C)|FD-Score:5.21|P-value:9.22E-8|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SMD3(YLR147C)|FD-Score:-3.2|P-value:6.77E-4|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC105(YGL093W)|FD-Score:5.06|P-value:2.09E-7|Clearance:0.13||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSS1(YDR086C)|FD-Score:12.1|P-value:4.55E-34|Clearance:0.52||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:STH1(YIL126W)|FD-Score:5.26|P-value:7.37E-8|Clearance:0.02||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUI1(YNL244C)|FD-Score:4.71|P-value:1.25E-6|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TFA1(YKL028W)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.01||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF34(YMR146C)|FD-Score:-4.64|P-value:1.77E-6|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIF35(YDR429C)|FD-Score:-3.76|P-value:8.56E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM22(YNL131W)|FD-Score:3.19|P-value:7.02E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:UTP23(YOR004W)|FD-Score:-3.15|P-value:8.03E-4|Clearance:0||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein Gene:UTP4(YDR324C)|FD-Score:-3.89|P-value:5.11E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YBR190W(YBR190W_d)|FD-Score:-4.01|P-value:3.09E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:4.3|P-value:8.63E-6|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR115C(YGR115C_d)|FD-Score:4.93|P-value:4.18E-7|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL195C(YJL195C_d)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YOR146W(YOR146W_d)|FD-Score:-4.38|P-value:5.92E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:YPL142C(YPL142C_d)|FD-Score:-3.4|P-value:3.39E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YPL238C(YPL238C_d)|FD-Score:-4.01|P-value:3.00E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:4.33|P-value:7.39E-6|Clearance:0.03||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4306703
Download HOP data (tab-delimited text)  (excel)
Gene:AIM2(YAL049C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor Gene:AIM32(YML050W)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALO1(YML086C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:ANT1(YPR128C)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARO1(YDR127W)|FD-Score:-5.87|P-value:2.21E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:-4.48|P-value:3.75E-6||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATP2(YJR121W)|FD-Score:4.88|P-value:5.44E-7||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:AVT2(YEL064C)|FD-Score:3.94|P-value:4.03E-5||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCK1(YJL095W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CAF16(YFL028C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CDC50(YCR094W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKB2(YOR039W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLB4(YLR210W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:CLB5(YPR120C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COS1(YNL336W)|FD-Score:5.29|P-value:6.28E-8||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CSM1(YCR086W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CTF19(YPL018W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CTI6(YPL181W)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DAP1(YPL170W)|FD-Score:6.99|P-value:1.39E-12||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DCN1(YLR128W)|FD-Score:6.16|P-value:3.54E-10||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DFG16(YOR030W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DIT2(YDR402C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:EAR1(YMR171C)|FD-Score:4.77|P-value:9.35E-7||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EHD3(YDR036C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:FMP48(YGR052W_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GAS5(YOL030W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall Gene:GIS3(YLR094C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Protein of unknown function Gene:GLO2(YDR272W)|FD-Score:4.3|P-value:8.47E-6||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:HOF1(YMR032W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HOP2(YGL033W)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HRD1(YOL013C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:IRC5(YFR038W)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-5.36|P-value:4.11E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JID1(YPR061C)|FD-Score:5.72|P-value:5.26E-9||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LSM1(YJL124C)|FD-Score:-4.46|P-value:4.07E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LTE1(YAL024C)|FD-Score:4.92|P-value:4.43E-7||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MDG1(YNL173C)|FD-Score:3.76|P-value:8.44E-5||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MDJ1(YFL016C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MHT1(YLL062C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:MSG5(YNL053W)|FD-Score:-3.88|P-value:5.30E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:NBP2(YDR162C)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NCS6(YGL211W)|FD-Score:-4.52|P-value:3.12E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NDL1(YLR254C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NEM1(YHR004C)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:OPI7(YDR360W_d)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PDX3(YBR035C)|FD-Score:5.05|P-value:2.24E-7||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PEX18(YHR160C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX32(YBR168W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO84(YML123C)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIM1(YBL022C)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PRO2(YOR323C)|FD-Score:6.99|P-value:1.42E-12||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAD59(YDL059C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RCY1(YJL204C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REI1(YBR267W)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIM101(YHL027W)|FD-Score:9.06|P-value:6.50E-20||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM20(YOR275C)|FD-Score:6.19|P-value:3.03E-10||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM21(YNL294C)|FD-Score:14|P-value:7.10E-45||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:15.3|P-value:3.82E-53||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPA49(YNL248C)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPS6B(YBR181C)|FD-Score:-5.18|P-value:1.10E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTG1(YOL067C)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SAG1(YJR004C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SAN1(YDR143C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SEA4(YBL104C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SFL1(YOR140W)|FD-Score:-4.45|P-value:4.33E-6||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SLT2(YHR030C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF7(YLR025W)|FD-Score:11.8|P-value:1.27E-32||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:6.91|P-value:2.45E-12||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPT3(YDR392W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT4(YGR063C)|FD-Score:-3.81|P-value:7.04E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRF1(YDL133W)|FD-Score:-3.18|P-value:7.24E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSN2(YDR443C)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:STP1(YDR463W)|FD-Score:-4.44|P-value:4.42E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:STP22(YCL008C)|FD-Score:4.37|P-value:6.17E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUS1(YBR111W-A)|FD-Score:-4.89|P-value:5.03E-7||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TAT1(YBR069C)|FD-Score:-6.25|P-value:2.10E-10||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TOM1(YDR457W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOM7(YNL070W)|FD-Score:-3.83|P-value:6.43E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRM1(YDR120C)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRP3(YKL211C)|FD-Score:-4.05|P-value:2.60E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-4.4|P-value:5.48E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSA1(YML028W)|FD-Score:5.45|P-value:2.56E-8||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:UAF30(YOR295W)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:URA2(YJL130C)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VMA1(YDL185W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:-4.76|P-value:9.70E-7||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS20(YMR077C)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:7.27|P-value:1.75E-13||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:12.7|P-value:4.45E-37||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:-3.79|P-value:7.56E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL071C(YBL071C_d)|FD-Score:-6.15|P-value:3.98E-10||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR071W(YBR071W)|FD-Score:7.37|P-value:8.24E-14||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YCR064C(YCR064C_d)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL118W(YDL118W_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL241W(YDL241W_p)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER046W-A(YER046W-A_d)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR064W(YGR064W_d)|FD-Score:-4.07|P-value:2.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR069W(YGR069W_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR122W(YGR122W)|FD-Score:5.55|P-value:1.45E-8||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIL092W(YIL092W_p)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL215C(YJL215C_d)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL077W(YKL077W_p)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLL044W(YLL044W_d)|FD-Score:-5.05|P-value:2.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR111W(YLR111W_d)|FD-Score:4.8|P-value:7.77E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:8.7|P-value:1.63E-18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML122C(YML122C_d)|FD-Score:-4.27|P-value:9.98E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR005C(YNR005C_d)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOP1(YPR028W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR121C(YOR121C_d)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPK1(YKL126W)|FD-Score:6.38|P-value:8.99E-11||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL034W(YPL034W_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPT6(YLR262C)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSP1(YHR155W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:AIM2(YAL049C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor Gene:AIM32(YML050W)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALO1(YML086C)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:ANT1(YPR128C)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARO1(YDR127W)|FD-Score:-5.87|P-value:2.21E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:-4.48|P-value:3.75E-6||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATP2(YJR121W)|FD-Score:4.88|P-value:5.44E-7||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:AVT2(YEL064C)|FD-Score:3.94|P-value:4.03E-5||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCK1(YJL095W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CAF16(YFL028C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CDC50(YCR094W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKB2(YOR039W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLB4(YLR210W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:CLB5(YPR120C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COS1(YNL336W)|FD-Score:5.29|P-value:6.28E-8||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CSM1(YCR086W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CTF19(YPL018W)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CTI6(YPL181W)|FD-Score:3.81|P-value:6.93E-5||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DAP1(YPL170W)|FD-Score:6.99|P-value:1.39E-12||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DCN1(YLR128W)|FD-Score:6.16|P-value:3.54E-10||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DFG16(YOR030W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DIT2(YDR402C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:N-formyltyrosine oxidase, sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s Gene:EAR1(YMR171C)|FD-Score:4.77|P-value:9.35E-7||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EHD3(YDR036C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis Gene:FMP48(YGR052W_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GAS5(YOL030W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall Gene:GIS3(YLR094C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Protein of unknown function Gene:GLO2(YDR272W)|FD-Score:4.3|P-value:8.47E-6||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:HOF1(YMR032W)|FD-Score:-3.1|P-value:9.72E-4||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HOP2(YGL033W)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HRD1(YOL013C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:IRC5(YFR038W)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-5.36|P-value:4.11E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JID1(YPR061C)|FD-Score:5.72|P-value:5.26E-9||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LSM1(YJL124C)|FD-Score:-4.46|P-value:4.07E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LTE1(YAL024C)|FD-Score:4.92|P-value:4.43E-7||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MDG1(YNL173C)|FD-Score:3.76|P-value:8.44E-5||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MDJ1(YFL016C)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MHT1(YLL062C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:MSG5(YNL053W)|FD-Score:-3.88|P-value:5.30E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:NBP2(YDR162C)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NCS6(YGL211W)|FD-Score:-4.52|P-value:3.12E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NDL1(YLR254C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NEM1(YHR004C)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:OPI7(YDR360W_d)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PDX3(YBR035C)|FD-Score:5.05|P-value:2.24E-7||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PEX18(YHR160C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX32(YBR168W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO84(YML123C)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PIM1(YBL022C)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PRO2(YOR323C)|FD-Score:6.99|P-value:1.42E-12||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAD59(YDL059C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RCY1(YJL204C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REI1(YBR267W)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIM101(YHL027W)|FD-Score:9.06|P-value:6.50E-20||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM20(YOR275C)|FD-Score:6.19|P-value:3.03E-10||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM21(YNL294C)|FD-Score:14|P-value:7.10E-45||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:15.3|P-value:3.82E-53||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPA49(YNL248C)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:RNA polymerase I subunit A49 Gene:RPS6B(YBR181C)|FD-Score:-5.18|P-value:1.10E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTG1(YOL067C)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SAG1(YJR004C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SAN1(YDR143C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SEA4(YBL104C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SFL1(YOR140W)|FD-Score:-4.45|P-value:4.33E-6||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SLT2(YHR030C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF7(YLR025W)|FD-Score:11.8|P-value:1.27E-32||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:6.91|P-value:2.45E-12||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPT3(YDR392W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT4(YGR063C)|FD-Score:-3.81|P-value:7.04E-5||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRF1(YDL133W)|FD-Score:-3.18|P-value:7.24E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSN2(YDR443C)|FD-Score:-4.05|P-value:2.58E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:STP1(YDR463W)|FD-Score:-4.44|P-value:4.42E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:STP22(YCL008C)|FD-Score:4.37|P-value:6.17E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUS1(YBR111W-A)|FD-Score:-4.89|P-value:5.03E-7||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TAT1(YBR069C)|FD-Score:-6.25|P-value:2.10E-10||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TOM1(YDR457W)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOM7(YNL070W)|FD-Score:-3.83|P-value:6.43E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRM1(YDR120C)|FD-Score:-3.15|P-value:8.20E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRP3(YKL211C)|FD-Score:-4.05|P-value:2.60E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-4.4|P-value:5.48E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSA1(YML028W)|FD-Score:5.45|P-value:2.56E-8||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:UAF30(YOR295W)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:URA2(YJL130C)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VMA1(YDL185W)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:-4.76|P-value:9.70E-7||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS20(YMR077C)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:7.27|P-value:1.75E-13||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:12.7|P-value:4.45E-37||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:-3.79|P-value:7.56E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL071C(YBL071C_d)|FD-Score:-6.15|P-value:3.98E-10||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR071W(YBR071W)|FD-Score:7.37|P-value:8.24E-14||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YCR064C(YCR064C_d)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL118W(YDL118W_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL241W(YDL241W_p)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Putative protein of unknown function; YDL241W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER046W-A(YER046W-A_d)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR064W(YGR064W_d)|FD-Score:-4.07|P-value:2.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR069W(YGR069W_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR122W(YGR122W)|FD-Score:5.55|P-value:1.45E-8||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIL092W(YIL092W_p)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YJL215C(YJL215C_d)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL077W(YKL077W_p)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YLL044W(YLL044W_d)|FD-Score:-5.05|P-value:2.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR111W(YLR111W_d)|FD-Score:4.8|P-value:7.77E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:8.7|P-value:1.63E-18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YML122C(YML122C_d)|FD-Score:-4.27|P-value:9.98E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR005C(YNR005C_d)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOP1(YPR028W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR121C(YOR121C_d)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPK1(YKL126W)|FD-Score:6.38|P-value:8.99E-11||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL034W(YPL034W_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPT6(YLR262C)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSP1(YHR155W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL205C15.509.12E-553.43HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YDR086C12.104.55E-340.52SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YDR224C11.602.18E-310.62HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YKR008W11.002.51E-281.19RSC4Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
YDR232W9.786.83E-231.98HEM15-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
YLR033W7.803.06E-150.08RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YMR043W7.735.57E-150.12MCM1Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
YPR034W7.611.37E-141.00ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YDR047W6.611.88E-110.35HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YJL039C6.271.86E-100.00NUP192Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205
YEL034W6.261.91E-100.23HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YGL040C6.038.41E-100.24HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YER125W5.783.66E-90.02RSP5E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p
YFR037C5.774.00E-90.10RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YBR192W5.677.17E-90.22RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL045W15.303.82E-53RIM8Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
YNL294C14.007.10E-45RIM21pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
YLR417W12.704.45E-37VPS36Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
YLR025W11.801.27E-32SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YHL027W9.066.50E-20RIM101Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
YML012C-A_d8.701.63E-18YML012C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
YBR071W7.378.24E-14YBR071WProtein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking
YJR102C7.271.75E-13VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YPL170W6.991.39E-12DAP1Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
YOR323C6.991.42E-12PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YPL002C6.912.45E-12SNF8Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
YKL126W6.388.99E-11YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YOR275C6.193.03E-10RIM20Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
YLR128W6.163.54E-10DCN1Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation
YMR077C5.921.58E-9VPS20Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes

GO enrichment analysis for SGTC_1044
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3634.07E-183SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.0882353heme biosynthesis & mitochondrial translocase
0.3519.63E-171SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.1heme biosynthesis & mitochondrial translocase
0.3441.29E-163SGTC_18845633444 25.0 μMMiscellaneous57242390.161765redox potentiating
0.3413.35E-160SGTC_29289011484 9.8 μMChembridge (Drug-like library)29947510.0810811heme biosynthesis & mitochondrial translocase
0.3384.70E-158SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.084507heme biosynthesis & mitochondrial translocase
0.3292.64E-149SGTC_525k781-2675 26.7 μMChemDiv (Drug-like library)36511180.140625redox potentiating
0.2953.08E-119SGTC_5761073-0113 22.7 μMChemDiv (Drug-like library)54395630.194805redox potentiating
0.2955.03E-119SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.0595238heme biosynthesis & mitochondrial translocase
0.2701.01E-98SGTC_9021120-0002 2.1 μMChemDiv (Drug-like library)32804750.0666667azole & statin
0.2571.79E-89SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.1superoxide
0.2511.58E-85SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.114754heme biosynthesis & mitochondrial translocase
0.2501.20E-84SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0933333heme biosynthesis & mitochondrial translocase
0.2482.32E-83SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.169492azole & statin
0.2464.48E-82SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0645161heme biosynthesis & mitochondrial translocase
0.2459.05E-82SGTC_13101188-0297 994.0 nMChemDiv (Drug-like library)15883520.0853659azole & statin

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1121fusaric acid630.51 nM0.53442TimTec (Natural product library)179.215722.59213
SGTC_361000s-05101.45 μM0.490196660ChemDiv (Drug-like library)148.158621.92302
SGTC_9601222-01867.7 μM0.4464293126296ChemDiv (Drug-like library)357.572567.00402
SGTC_9551216-011033 μM0.3753619719ChemDiv (Drug-like library)290.397245.55813sphingolipid biosynthesis & PDR1
SGTC_13141222-001041.1 μM0.3214293526027ChemDiv (Drug-like library)251.321443.98514
SGTC_980074-0023107.66 μM0.2678573563821ChemDiv (Drug-like library)278.343484.63114Golgi
SGTC_2248tofa1.1 μM0.263158115175Miscellaneous324.454986.85314sphingolipid biosynthesis & PDR1
SGTC_2416tofa880 nM0.263158115175Miscellaneous324.454986.85314sphingolipid biosynthesis & PDR1
SGTC_820054-0107293.76 μM0.253104697ChemDiv (Drug-like library)340.412866.11514
SGTC_2961222-003974.9 μM0.255984176ChemDiv (Drug-like library)218.291524.22812
SGTC_790052-000794.09 μM0.2456142801472ChemDiv (Drug-like library)276.370664.63813