2001-0062

2-[1-[2-(2-methylphenoxy)ethyl]benzimidazol-2-yl]sulfanylacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1046
Screen concentration 216.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 739778
SMILES CC1=CC=CC=C1OCCN2C3=CC=CC=C3N=C2SCC(=O)N
Standardized SMILES Cc1ccccc1OCCn2c(SCC(=O)N)nc3ccccc23
Molecular weight 341.4274
ALogP 3.61
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.82
% growth inhibition (Hom. pool) 2.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 739778
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:3.86|P-value:5.78E-5|Clearance:0.21||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDS1(YBR029C)|FD-Score:3.18|P-value:7.24E-4|Clearance:0.06||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:DCP2(YNL118C)|FD-Score:-4.37|P-value:6.34E-6|Clearance:0||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:GPI11(YDR302W)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.17||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:PRP11(YDL043C)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.06||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RPM2(YML091C)|FD-Score:3.12|P-value:8.92E-4|Clearance:0||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RTS2(YOR077W)|FD-Score:-3.34|P-value:4.15E-4|Clearance:0||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SAD1(YFR005C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.4||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SCL1(YGL011C)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.04||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SOG2(YOR353C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.18||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:TAP42(YMR028W)|FD-Score:-3.16|P-value:8.00E-4|Clearance:0||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:TPT1(YOL102C)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide Gene:YDR341C(YDR341C)|FD-Score:3.22|P-value:6.32E-4|Clearance:0.04||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:BMS1(YPL217C)|FD-Score:3.86|P-value:5.78E-5|Clearance:0.21||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CDS1(YBR029C)|FD-Score:3.18|P-value:7.24E-4|Clearance:0.06||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:DCP2(YNL118C)|FD-Score:-4.37|P-value:6.34E-6|Clearance:0||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:GPI11(YDR302W)|FD-Score:3.4|P-value:3.39E-4|Clearance:0.17||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:PRP11(YDL043C)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.06||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RPM2(YML091C)|FD-Score:3.12|P-value:8.92E-4|Clearance:0||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RTS2(YOR077W)|FD-Score:-3.34|P-value:4.15E-4|Clearance:0||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SAD1(YFR005C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.4||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SCL1(YGL011C)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.04||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SOG2(YOR353C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.18||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:TAP42(YMR028W)|FD-Score:-3.16|P-value:8.00E-4|Clearance:0||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:TPT1(YOL102C)|FD-Score:-3.29|P-value:5.01E-4|Clearance:0||SGD DESC:tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide Gene:YDR341C(YDR341C)|FD-Score:3.22|P-value:6.32E-4|Clearance:0.04||SGD DESC:Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 739778
Download HOP data (tab-delimited text)  (excel)
Gene:ALP1(YNL270C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:AMS1(YGL156W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ATP23(YNR020C)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BEM4(YPL161C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CBR1(YIL043C)|FD-Score:6.25|P-value:2.12E-10||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CBS2(YDR197W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CCC1(YLR220W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:COX7(YMR256C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:ERP6(YGL002W)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:FLO1(YAR050W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:HAT1(YPL001W)|FD-Score:4.99|P-value:3.10E-7||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HOG1(YLR113W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HSE1(YHL002W)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IRC19(YLL033W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITR2(YOL103W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:LDB7(YBL006C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MDL2(YPL270W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MIG1(YGL035C)|FD-Score:4.85|P-value:6.22E-7||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MLS1(YNL117W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:NAM7(YMR080C)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:PCL9(YDL179W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PET494(YNR045W)|FD-Score:-4.35|P-value:6.89E-6||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX30(YLR324W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHM8(YER037W)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PIN4(YBL051C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POM152(YMR129W)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 Gene:PPN1(YDR452W)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:QRI5(YLR204W)|FD-Score:5.51|P-value:1.78E-8||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RPN13(YLR421C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SCW11(YGL028C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SED4(YCR067C)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SER3(YER081W)|FD-Score:-4.49|P-value:3.62E-6||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SNA4(YDL123W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SOK1(YDR006C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPE2(YOL052C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:TAL1(YLR354C)|FD-Score:6.51|P-value:3.86E-11||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TDP1(YBR223C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TOF1(YNL273W)|FD-Score:-4.64|P-value:1.71E-6||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TPS3(YMR261C)|FD-Score:-5.01|P-value:2.70E-7||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:VID24(YBR105C)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA3(YEL027W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:YDR401W(YDR401W_d)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEF1(YEL041W)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YFL032W(YFL032W_d)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YGR219W(YGR219W_d)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL037C(YHL037C_d)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR022C(YHR022C_p)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YHR182W(YHR182W_p)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIH1(YCR059C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YKL077W(YKL077W_p)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL102C(YKL102C_d)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YKR104W(YKR104W)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLF2(YHL014C)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR278C(YLR278C_p)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YML096W(YML096W_p)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR141C(YMR141C_d)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL046C(YOL046C_d)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR029W(YOR029W_d)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL168W(YPL168W_p)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR027C(YPR027C_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function Gene:YPR050C(YPR050C_d)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPT52(YKR014C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YTP1(YNL237W)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ALP1(YNL270C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:AMS1(YGL156W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ATP23(YNR020C)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BEM4(YPL161C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CBR1(YIL043C)|FD-Score:6.25|P-value:2.12E-10||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CBS2(YDR197W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:CCC1(YLR220W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:COX7(YMR256C)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:ERP6(YGL002W)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:FLO1(YAR050W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:HAT1(YPL001W)|FD-Score:4.99|P-value:3.10E-7||SGD DESC:Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair Gene:HOG1(YLR113W)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HSE1(YHL002W)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:IRC19(YLL033W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:ITR2(YOL103W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:LDB7(YBL006C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MDL2(YPL270W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MIG1(YGL035C)|FD-Score:4.85|P-value:6.22E-7||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MLS1(YNL117W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:NAM7(YMR080C)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:PCL9(YDL179W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PET494(YNR045W)|FD-Score:-4.35|P-value:6.89E-6||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX30(YLR324W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHM8(YER037W)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PIN4(YBL051C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POM152(YMR129W)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 Gene:PPN1(YDR452W)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:QRI5(YLR204W)|FD-Score:5.51|P-value:1.78E-8||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RPN13(YLR421C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SCW11(YGL028C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SED4(YCR067C)|FD-Score:-4.05|P-value:2.54E-5||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SER3(YER081W)|FD-Score:-4.49|P-value:3.62E-6||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SNA4(YDL123W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SOK1(YDR006C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPE2(YOL052C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:TAL1(YLR354C)|FD-Score:6.51|P-value:3.86E-11||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TDP1(YBR223C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TOF1(YNL273W)|FD-Score:-4.64|P-value:1.71E-6||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TPS3(YMR261C)|FD-Score:-5.01|P-value:2.70E-7||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:VID24(YBR105C)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA3(YEL027W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:YDR401W(YDR401W_d)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEF1(YEL041W)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YFL032W(YFL032W_d)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YGR219W(YGR219W_d)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL037C(YHL037C_d)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR022C(YHR022C_p)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Putative protein of unknown function; YHR022C is not an essential gene Gene:YHR182W(YHR182W_p)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIH1(YCR059C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YKL077W(YKL077W_p)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YKL102C(YKL102C_d)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YKR104W(YKR104W)|FD-Score:-3.21|P-value:6.64E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLF2(YHL014C)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR278C(YLR278C_p)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YML096W(YML096W_p)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR141C(YMR141C_d)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL046C(YOL046C_d)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YOR029W(YOR029W_d)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL168W(YPL168W_p)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR027C(YPR027C_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function Gene:YPR050C(YPR050C_d)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPT52(YKR014C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YTP1(YNL237W)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFR005C4.261.03E-50.40SAD1Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
YPL217C3.865.78E-50.21BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YOR353C3.641.35E-40.18SOG2Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
YDL043C3.462.73E-40.06PRP11Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YDR302W3.403.39E-40.17GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YDR341C3.226.32E-40.04YDR341CArginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YBR029C3.187.24E-40.06CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YML091C3.128.92E-40.00RPM2Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
YGL011C3.129.00E-40.04SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YGL123W3.080.001020.06RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YDR324C3.020.001250.01UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YGR145W3.010.001300.07ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YGR099W2.940.001660.16TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YMR185W_p2.770.002760.05RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YDL105W2.730.003200.01NSE4Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR354C6.513.86E-11TAL1Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
YIL043C6.252.12E-10CBR1Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia
YLR204W5.511.78E-8QRI5Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
YLR278C_p5.081.93E-7YLR278C_pZinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene
YPL001W4.993.10E-7HAT1Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex that uses the cofactor acetyl coenzyme A, to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair
YGL035C4.856.22E-7MIG1Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
YNL237W4.751.01E-6YTP1Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
YHR182W_p4.681.43E-6YHR182W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress
YGL028C4.552.69E-6SCW11Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p
YEL041W4.483.79E-6YEF1ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
YCR059C4.261.00E-5YIH1Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
YKL126W4.251.06E-5YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR264W4.211.25E-5RPS28BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
YDR401W_d4.062.47E-5YDR401W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR223C4.042.67E-5TDP1Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI

GO enrichment analysis for SGTC_1046
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0978.96E-14SGTC_2762leflunomide 42.1 μMMiscellaneous38990.121622
0.0857.44E-11SGTC_22287354201 200.0 μMChembridge (Fragment library)7534510.08endomembrane recycling
0.0831.95E-10SGTC_760433-0009 400.3 μMChemDiv (Drug-like library)28415690.142857
0.0741.41E-8SGTC_20795212404 121.0 μMChembridge (Fragment library)7584620.0588235
0.0741.53E-8SGTC_10573448-5381 134.0 μMChemDiv (Drug-like library)54979110.0933333calcium & mitochondrial duress
0.0723.54E-8SGTC_12330325-0408 14.2 μMChemDiv (Drug-like library)128740.1
0.0714.61E-8SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0869565
0.0706.73E-8SGTC_1220236-0016 402.4 μMChemDiv (Drug-like library)68088380.0869565RNA processing & uracil transport
0.0691.25E-7SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.104651Golgi
0.0681.68E-7SGTC_5733702-0553 84.7 μMChemDiv (Drug-like library)12227560.0757576
0.0672.61E-7SGTC_30059076496 71.4 μMChembridge (Drug-like library)213684100.105263
0.0672.83E-7SGTC_32509135583 49.5 μMChembridge (Drug-like library)76993530.202899
0.0612.37E-6SGTC_1712st032288 53.4 μMTimTec (Natural product derivative library)28830110.0384615calcium & mitochondrial duress
0.0595.89E-6SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.129412cell wall
0.0589.65E-6SGTC_20985376439 882.5 nMChembridge (Fragment library)28441160.0921053

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1974544-004341.56 μM0.396825896523ChemDiv (Drug-like library)322.189224.30713endomembrane recycling
SGTC_2862902763471.43 μM0.3823535012562Chembridge (Drug-like library)324.416764.45213ERG2
SGTC_2987901833871.43 μM0.3582095055682Chembridge (Drug-like library)330.808663.98313RPP1 & pyrimidine depletion
SGTC_7223534-0461190 μM0.353846658262ChemDiv (Drug-like library)293.362963.9512PDR1
SGTC_3011908127471.43 μM0.3243246625322Chembridge (Drug-like library)322.400884.26303
SGTC_13702001-009244.6 μM0.322581790957ChemDiv (Drug-like library)256.2749233.38903
SGTC_22937624732200 μM0.31746894453Chembridge (Fragment library)222.26361.77914
SGTC_3288911969036.22 μM0.31343317595618Chembridge (Drug-like library)295.378843.00702
SGTC_3017908068149.47 μM0.30882416451914Chembridge (Drug-like library)307.389543.14702
SGTC_2857902469158.44 μM0.30263216478292Chembridge (Drug-like library)418.412253.77907