3253-3903

3-(4-bromophenyl)-N-[(Z)-(2,6-dichlorophenyl)methylideneamino]-1H-pyrazole-5-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1051
Screen concentration 78.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5509239
SMILES C1=CC(=C(C(=C1)Cl)C=NNC(=O)C2=CC(=NN2)C3=CC=C(C=C3)Br)Cl
Standardized SMILES Clc1cccc(Cl)c1C=NNC(=O)c2cc([nH]n2)c3ccc(Br)cc3
Molecular weight 438.1054
ALogP 5.44
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.55
% growth inhibition (Hom. pool) 3.58


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5509239
Download HIP data (tab-delimited text)  (excel)
Gene:ARC15(YIL062C)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.31||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:CCT5(YJR064W)|FD-Score:4.01|P-value:3.08E-5|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:MTR3(YGR158C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NBP1(YLR457C)|FD-Score:-3.94|P-value:4.11E-5|Clearance:0||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NUP49(YGL172W)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.06||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PHS1(YJL097W)|FD-Score:-3.87|P-value:5.44E-5|Clearance:0||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:PUP1(YOR157C)|FD-Score:3.81|P-value:6.86E-5|Clearance:0.26||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFT1(YBL020W)|FD-Score:-4.03|P-value:2.83E-5|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RIX1(YHR197W)|FD-Score:-3.35|P-value:4.06E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPA190(YOR341W)|FD-Score:5.27|P-value:6.88E-8|Clearance:1.02||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPB10(YOR210W)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RRN3(YKL125W)|FD-Score:5.09|P-value:1.77E-7|Clearance:1.02||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SEC21(YNL287W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SPB1(YCL054W)|FD-Score:5.02|P-value:2.55E-7|Clearance:1.02||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPP382(YLR424W)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.09||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:STU1(YBL034C)|FD-Score:-3.45|P-value:2.79E-4|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:TAF12(YDR145W)|FD-Score:3.15|P-value:8.27E-4|Clearance:0.04||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:THI80(YOR143C)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.01||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:YDL016C(YDL016C_d)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YKL111C(YKL111C_d)|FD-Score:-4.46|P-value:4.17E-6|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:ARC15(YIL062C)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.31||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:CCT5(YJR064W)|FD-Score:4.01|P-value:3.08E-5|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:MTR3(YGR158C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NBP1(YLR457C)|FD-Score:-3.94|P-value:4.11E-5|Clearance:0||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NUP49(YGL172W)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.06||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PHS1(YJL097W)|FD-Score:-3.87|P-value:5.44E-5|Clearance:0||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:PUP1(YOR157C)|FD-Score:3.81|P-value:6.86E-5|Clearance:0.26||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFT1(YBL020W)|FD-Score:-4.03|P-value:2.83E-5|Clearance:0||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RIX1(YHR197W)|FD-Score:-3.35|P-value:4.06E-4|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPA190(YOR341W)|FD-Score:5.27|P-value:6.88E-8|Clearance:1.02||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPB10(YOR210W)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RRN3(YKL125W)|FD-Score:5.09|P-value:1.77E-7|Clearance:1.02||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SEC21(YNL287W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SPB1(YCL054W)|FD-Score:5.02|P-value:2.55E-7|Clearance:1.02||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPP382(YLR424W)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.09||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:STU1(YBL034C)|FD-Score:-3.45|P-value:2.79E-4|Clearance:0||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:TAF12(YDR145W)|FD-Score:3.15|P-value:8.27E-4|Clearance:0.04||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:THI80(YOR143C)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.01||SGD DESC:Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) Gene:YDL016C(YDL016C_d)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YKL111C(YKL111C_d)|FD-Score:-4.46|P-value:4.17E-6|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5509239
Download HOP data (tab-delimited text)  (excel)
Gene:AKR1(YDR264C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARO4(YBR249C)|FD-Score:-3.72|P-value:9.78E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:BTN2(YGR142W)|FD-Score:4.56|P-value:2.56E-6||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.49|P-value:3.61E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CMK2(YOL016C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COR1(YBL045C)|FD-Score:-3.84|P-value:6.24E-5||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:CTF19(YPL018W)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CTI6(YPL181W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DAK2(YFL053W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DCS2(YOR173W)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance Gene:DIA1(YMR316W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ECL1(YGR146C)|FD-Score:4.86|P-value:5.78E-7||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ELP2(YGR200C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EXG1(YLR300W)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:GEP3(YOR205C)|FD-Score:5.73|P-value:5.15E-9||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GET2(YER083C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GIP3(YPL137C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:HMT1(YBR034C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HNT1(YDL125C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:INM1(YHR046C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IOC3(YFR013W)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC2(YDR112W_d)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:MAP1(YLR244C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MGM1(YOR211C)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:NIP100(YPL174C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NRP1(YDL167C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NTA1(YJR062C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:OCA1(YNL099C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PDR1(YGL013C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PIM1(YBL022C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PLB3(YOL011W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:RAD10(YML095C)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RNH70(YGR276C)|FD-Score:4.46|P-value:4.06E-6||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:ROX1(YPR065W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL35A(YDL191W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.82|P-value:7.10E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS7B(YNL096C)|FD-Score:4.41|P-value:5.09E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM28(YDR494W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SEY1(YOR165W)|FD-Score:4.84|P-value:6.51E-7||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFM1(YOR021C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SHH3(YMR118C_p)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SLM3(YDL033C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLO1(YER180C-A)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SPR1(YOR190W)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SPT10(YJL127C)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:TDP1(YBR223C)|FD-Score:6.83|P-value:4.14E-12||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TGL3(YMR313C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TIR4(YOR009W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:UBP5(YER144C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:URN1(YPR152C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VMA13(YPR036W)|FD-Score:5.86|P-value:2.35E-9||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA8(YEL051W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS15(YBR097W)|FD-Score:-4.98|P-value:3.14E-7||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:WSC2(YNL283C)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YAR029W(YAR029W_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR064C(YCR064C_d)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YFL041W-A(YFL041W-A_p)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIH1(YCR059C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJL218W(YJL218W_p)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YKR070W(YKR070W_p)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR036C(YLR036C_p)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR358C(YLR358C_p)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Putative protein of unknown function Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function Gene:YMR244W(YMR244W_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Putative protein of unknown function Gene:YPL014W(YPL014W_p)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YSR3(YKR053C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:AKR1(YDR264C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARO4(YBR249C)|FD-Score:-3.72|P-value:9.78E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:BTN2(YGR142W)|FD-Score:4.56|P-value:2.56E-6||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.49|P-value:3.61E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CMK2(YOL016C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COR1(YBL045C)|FD-Score:-3.84|P-value:6.24E-5||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:CTF19(YPL018W)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:CTI6(YPL181W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DAK2(YFL053W)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DCS2(YOR173W)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance Gene:DIA1(YMR316W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ECL1(YGR146C)|FD-Score:4.86|P-value:5.78E-7||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ELP2(YGR200C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:EXG1(YLR300W)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:GEP3(YOR205C)|FD-Score:5.73|P-value:5.15E-9||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GET2(YER083C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GIP3(YPL137C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:HMT1(YBR034C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HNT1(YDL125C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:INM1(YHR046C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IOC3(YFR013W)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC2(YDR112W_d)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:MAP1(YLR244C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MGM1(YOR211C)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mt morphology and genome maintenance; exists as long and short form with different distributions; homolog of human OPA1 involved in autosomal dominant optic atrophy Gene:NIP100(YPL174C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NRP1(YDL167C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:NTA1(YJR062C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:OCA1(YNL099C)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PDR1(YGL013C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PIM1(YBL022C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PLB3(YOL011W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:RAD10(YML095C)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RNH70(YGR276C)|FD-Score:4.46|P-value:4.06E-6||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:ROX1(YPR065W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL35A(YDL191W)|FD-Score:4|P-value:3.17E-5||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.82|P-value:7.10E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS7B(YNL096C)|FD-Score:4.41|P-value:5.09E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSM28(YDR494W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SEY1(YOR165W)|FD-Score:4.84|P-value:6.51E-7||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFM1(YOR021C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SHH3(YMR118C_p)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SLM3(YDL033C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLO1(YER180C-A)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SPR1(YOR190W)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SPT10(YJL127C)|FD-Score:4.61|P-value:2.04E-6||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:TDP1(YBR223C)|FD-Score:6.83|P-value:4.14E-12||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TGL3(YMR313C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TIR4(YOR009W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock Gene:UBP5(YER144C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:URN1(YPR152C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VMA13(YPR036W)|FD-Score:5.86|P-value:2.35E-9||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA8(YEL051W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS15(YBR097W)|FD-Score:-4.98|P-value:3.14E-7||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:WSC2(YNL283C)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YAR029W(YAR029W_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR064C(YCR064C_d)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YFL041W-A(YFL041W-A_p)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIH1(YCR059C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJL218W(YJL218W_p)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YKR070W(YKR070W_p)|FD-Score:-4.12|P-value:1.91E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR036C(YLR036C_p)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR358C(YLR358C_p)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Putative protein of unknown function Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function Gene:YMR244W(YMR244W_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Putative protein of unknown function Gene:YPL014W(YPL014W_p)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YSR3(YKR053C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR341W5.276.88E-81.02RPA190RNA polymerase I largest subunit A190
YKL125W5.091.77E-71.02RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YCL054W5.022.55E-71.02SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YJR064W4.013.08E-50.09CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR424W3.924.44E-50.09SPP382Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
YDL016C_d3.836.36E-50.02YDL016C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
YOR157C3.816.86E-50.26PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YGR158C3.551.91E-40.03MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YOR143C3.522.14E-40.01THI80Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate)
YIL062C3.512.20E-40.31ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YGL172W3.216.74E-40.06NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YDR145W3.158.27E-40.04TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YOR210W3.119.45E-40.08RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YPR178W3.020.001250.11PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YDL058W2.910.001790.03USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR223C6.834.14E-12TDP1Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI
YPR036W5.862.35E-9VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
YOR205C5.735.15E-9GEP3Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1)
YOR190W5.462.38E-8SPR1Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance
YGR146C4.865.78E-7ECL1Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation
YOR165W4.846.51E-7SEY1Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
YDL020C4.827.10E-7RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YFL041W-A_p4.661.57E-6YFL041W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YFR013W4.612.01E-6IOC3Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
YJL127C4.612.04E-6SPT10Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box
YGR142W4.562.56E-6BTN2v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication
YPL069C4.493.61E-6BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YDR264C4.473.94E-6AKR1Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication
YGR276C4.464.06E-6RNH703'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts
YNL096C4.415.09E-6RPS7BProtein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1051
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0831.63E-10SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.119403
0.0808.55E-10SGTC_489paxilline 115.0 μMICCB bioactive library1050080.0594059PDR1
0.0791.21E-9SGTC_10523253-3998 198.0 μMChemDiv (Drug-like library)54503170.405797
0.0782.24E-9SGTC_2765myclobutanil 7.7 μMMiscellaneous63360.0731707azole & statin
0.0756.76E-9SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0526316PDR1
0.0757.58E-9SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0757576PDR1
0.0741.41E-8SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.157303PDR1
0.0732.18E-8SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.191011PDR1
0.0672.39E-7SGTC_417st075190 100.0 μMTimTec (Pure natural product library)2227570.0879121
0.0673.30E-7SGTC_29025787006 60.5 μMChembridge (Drug-like library)5910030.146667Golgi
0.0664.18E-7SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.0595238
0.0664.49E-7SGTC_31159123671 49.5 μMChembridge (Drug-like library)32869880.142857
0.0655.58E-7SGTC_1660st012960 28.1 μMTimTec (Natural product derivative library)242072660.0864198iron homeostasis
0.0655.89E-7SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0461538
0.0647.18E-7SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.120482

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12082323-234750.3 μM0.4843755412760ChemDiv (Drug-like library)346.405664.22724
SGTC_12092323-234750.3 μM0.4843755412760ChemDiv (Drug-like library)346.405664.22724
SGTC_10523253-3998198 μM0.4057975450317ChemDiv (Drug-like library)370.359322.71127
SGTC_13301469-005461.8 μM0.4027787102664ChemDiv (Drug-like library)382.80372.93536iron homeostasis
SGTC_8304076-028657.8 μM0.354436866659ChemDiv (Drug-like library)411.455844.26825translation
SGTC_7051124-0212310 μM0.3278699554485ChemDiv (Drug-like library)293.1484.01212
SGTC_6541124-027180.9 μM0.3181825403748ChemDiv (Drug-like library)309.115462.58524
SGTC_11780988-0033191 μM0.3134335392207ChemDiv (Drug-like library)349.179323.17324
SGTC_563tetrahydrosecaminediol614 μM0.304348334274ChemDiv (Drug-like library)624.898248.24734amide catabolism
SGTC_1166k206-0078225 μM0.3043485404595ChemDiv (Drug-like library)325.749083.40214
SGTC_24605552655174.22 μM0.3015875401989Miscellaneous259.6912.62513amide catabolism