| Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
|---|
| YBR154C | 5.76 | 4.22E-9 | 0.54 | RPB5 | RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation |
| YLR208W | 5.72 | 5.23E-9 | 0.54 | SEC13 | Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress |
| YGR265W_d | 5.19 | 1.08E-7 | 0.25 | YGR265W_d | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase |
| YGL001C | 4.94 | 3.99E-7 | 0.09 | ERG26 | C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis |
| YOR174W | 4.85 | 6.26E-7 | 0.37 | MED4 | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
| YPR165W | 4.48 | 3.75E-6 | 0.01 | RHO1 | GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) |
| YJR023C_d | 4.47 | 3.96E-6 | 0.34 | YJR023C_d | Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing |
| YIL021W | 4.13 | 1.81E-5 | 0.16 | RPB3 | RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit |
| YDR526C_d | 3.97 | 3.56E-5 | 0.08 | YDR526C_d | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
| YNL189W | 3.89 | 5.04E-5 | 0.18 | SRP1 | Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation |
| YLR197W | 3.71 | 1.04E-4 | 0.11 | NOP56 | Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects |
| YGL137W | 3.60 | 1.61E-4 | 0.08 | SEC27 | Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP |
| YDL008W | 3.52 | 2.16E-4 | 0.04 | APC11 | Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity |
| YFL038C | 3.48 | 2.47E-4 | 0.07 | YPT1 | Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) |
| YKL203C | 3.41 | 3.22E-4 | 0.03 | TOR2 | PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication |
