3448-6867

1-[4-[(2-chlorophenyl)methoxy]phenyl]-5-oxo-N-(oxolan-2-ylmethyl)pyrrolidine-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1059
Screen concentration 171.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3934407
SMILES C1CC(OC1)CNC(=O)C2CC(=O)N(C2)C3=CC=C(C=C3)OCC4=CC=CC=C4Cl
Standardized SMILES Clc1ccccc1COc2ccc(cc2)N3CC(CC3=O)C(=O)NCC4CCCO4
Molecular weight 428.9086
ALogP 2.9
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.27
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3934407
Download HIP data (tab-delimited text)  (excel)
Gene:CCT2(YIL142W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.09||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CEG1(YGL130W)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.14||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:ERG12(YMR208W)|FD-Score:-3.15|P-value:8.21E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI8(YDR331W)|FD-Score:3.94|P-value:4.10E-5|Clearance:0.07||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:MTR4(YJL050W)|FD-Score:3.8|P-value:7.24E-5|Clearance:0.25||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NOP56(YLR197W)|FD-Score:-3.15|P-value:8.23E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PDS1(YDR113C)|FD-Score:3.82|P-value:6.55E-5|Clearance:0.02||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:POP6(YGR030C)|FD-Score:3.37|P-value:3.69E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP4(YPR178W)|FD-Score:3.86|P-value:5.58E-5|Clearance:0.04||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPA135(YPR010C)|FD-Score:4.38|P-value:6.00E-6|Clearance:0.38||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RRP43(YCR035C)|FD-Score:-3.56|P-value:1.89E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SPC98(YNL126W)|FD-Score:3.3|P-value:4.84E-4|Clearance:0.03||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP382(YLR424W)|FD-Score:-3.15|P-value:8.30E-4|Clearance:0||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SUB2(YDL084W)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.05||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWD2(YKL018W)|FD-Score:4|P-value:3.15E-5|Clearance:0.06||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TFG2(YGR005C)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.06||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:YBR190W(YBR190W_d)|FD-Score:4.92|P-value:4.39E-7|Clearance:0.54||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YKL111C(YKL111C_d)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.04||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:CCT2(YIL142W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.09||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CEG1(YGL130W)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.14||SGD DESC:Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:ERG12(YMR208W)|FD-Score:-3.15|P-value:8.21E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GPI8(YDR331W)|FD-Score:3.94|P-value:4.10E-5|Clearance:0.07||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:MTR4(YJL050W)|FD-Score:3.8|P-value:7.24E-5|Clearance:0.25||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NOP56(YLR197W)|FD-Score:-3.15|P-value:8.23E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PDS1(YDR113C)|FD-Score:3.82|P-value:6.55E-5|Clearance:0.02||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:POP6(YGR030C)|FD-Score:3.37|P-value:3.69E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP4(YPR178W)|FD-Score:3.86|P-value:5.58E-5|Clearance:0.04||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPA135(YPR010C)|FD-Score:4.38|P-value:6.00E-6|Clearance:0.38||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RRP43(YCR035C)|FD-Score:-3.56|P-value:1.89E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SPC98(YNL126W)|FD-Score:3.3|P-value:4.84E-4|Clearance:0.03||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP382(YLR424W)|FD-Score:-3.15|P-value:8.30E-4|Clearance:0||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SUB2(YDL084W)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.05||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWD2(YKL018W)|FD-Score:4|P-value:3.15E-5|Clearance:0.06||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TFG2(YGR005C)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.06||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:YBR190W(YBR190W_d)|FD-Score:4.92|P-value:4.39E-7|Clearance:0.54||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YKL111C(YKL111C_d)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.04||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3934407
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ADD66(YKL206C)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:ADY2(YCR010C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AIM29(YKR074W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARI1(YGL157W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARO4(YBR249C)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ASI2(YNL159C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATE1(YGL017W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:ATP1(YBL099W)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CUE5(YOR042W)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DAK2(YFL053W)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DCS2(YOR173W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance Gene:DIF1(YLR437C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DLD1(YDL174C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane Gene:ENV7(YPL236C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:FIT2(YOR382W)|FD-Score:4.27|P-value:9.77E-6||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMP41(YNL168C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAS3(YMR215W)|FD-Score:5.95|P-value:1.34E-9||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:HMT1(YBR034C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HSP42(YDR171W)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IRE1(YHR079C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:KIP3(YGL216W)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning Gene:LAG1(YHL003C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:MCH1(YDL054C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MNN4(YKL201C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:NUT1(YGL151W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OPI9(YLR338W_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PEX31(YGR004W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PUS2(YGL063W)|FD-Score:-4.34|P-value:7.14E-6||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RPL24B(YGR148C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL8B(YLL045C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-5.02|P-value:2.65E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS23B(YPR132W)|FD-Score:-4.28|P-value:9.15E-6||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SAC3(YDR159W)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SCH9(YHR205W)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SED4(YCR067C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SER33(YIL074C)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SLM3(YDL033C)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SNF1(YDR477W)|FD-Score:-7.53|P-value:2.59E-14||SGD DESC:AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation Gene:SNF12(YNR023W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPO75(YLL005C)|FD-Score:4.47|P-value:3.83E-6||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:TPN1(YGL186C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UPS2(YLR168C)|FD-Score:-4.85|P-value:6.29E-7||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VMA11(YPL234C)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS35(YJL154C)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VTH1(YIL173W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL180W(YDL180W_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR274C(YDR274C_d)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER067C-A(YER067C-A_d)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YGR168C(YGR168C_p)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHL017W(YHL017W_p)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIA6(YIL006W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YLL020C(YLL020C_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YML131W(YML131W_p)|FD-Score:4.56|P-value:2.50E-6||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YMR294W-A(YMR294W-A_d)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:ABZ2(YMR289W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ADD66(YKL206C)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:ADY2(YCR010C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AIM29(YKR074W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARI1(YGL157W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARO4(YBR249C)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ASI2(YNL159C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATE1(YGL017W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:ATP1(YBL099W)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CUE5(YOR042W)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DAK2(YFL053W)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DCS2(YOR173W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance Gene:DIF1(YLR437C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DLD1(YDL174C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane Gene:ENV7(YPL236C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:FIT2(YOR382W)|FD-Score:4.27|P-value:9.77E-6||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMP41(YNL168C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAS3(YMR215W)|FD-Score:5.95|P-value:1.34E-9||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:HMT1(YBR034C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HSP42(YDR171W)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:IRE1(YHR079C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:KIP3(YGL216W)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning Gene:LAG1(YHL003C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:MCH1(YDL054C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MNN4(YKL201C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:NUT1(YGL151W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OPI9(YLR338W_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PEX31(YGR004W)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PUS2(YGL063W)|FD-Score:-4.34|P-value:7.14E-6||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RPL24B(YGR148C)|FD-Score:-4.02|P-value:2.90E-5||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL2B(YIL018W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPL8B(YLL045C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-5.02|P-value:2.65E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS23B(YPR132W)|FD-Score:-4.28|P-value:9.15E-6||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SAC3(YDR159W)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SCH9(YHR205W)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SED4(YCR067C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SER33(YIL074C)|FD-Score:-3.2|P-value:6.86E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SLM3(YDL033C)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SNF1(YDR477W)|FD-Score:-7.53|P-value:2.59E-14||SGD DESC:AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation Gene:SNF12(YNR023W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPO75(YLL005C)|FD-Score:4.47|P-value:3.83E-6||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:TPN1(YGL186C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UPS2(YLR168C)|FD-Score:-4.85|P-value:6.29E-7||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VMA11(YPL234C)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS35(YJL154C)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VTH1(YIL173W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL180W(YDL180W_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR274C(YDR274C_d)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER067C-A(YER067C-A_d)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YGR168C(YGR168C_p)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHL017W(YHL017W_p)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIA6(YIL006W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YLL020C(YLL020C_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YML131W(YML131W_p)|FD-Score:4.56|P-value:2.50E-6||SGD DESC:Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YMR294W-A(YMR294W-A_d)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR190W_d4.924.39E-70.54YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YPR010C4.386.00E-60.38RPA135RNA polymerase I second largest subunit A135
YKL018W4.003.15E-50.06SWD2Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
YDR331W3.944.10E-50.07GPI8ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
YPR178W3.865.58E-50.04PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YDR113C3.826.55E-50.02PDS1Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
YJL050W3.807.24E-50.25MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YGL130W3.551.92E-40.14CEG1Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphosphatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
YKL111C_d3.413.22E-40.04YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YGR030C3.373.69E-40.08POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YNL126W3.304.84E-40.03SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YGR005C3.275.47E-40.06TFG2TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
YDL084W3.216.64E-40.05SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YIL142W3.167.94E-40.09CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR201W3.070.001080.05SPC19Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR215W5.951.34E-9GAS3Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation
YLL020C_d4.641.74E-6YLL020C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1
YML131W_p4.562.50E-6YML131W_pProtein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
YBR249C4.532.95E-6ARO43-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress
YLL005C4.473.83E-6SPO75Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis
YHR205W4.415.15E-6SCH9AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
YOR382W4.279.77E-6FIT2Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
YLR437C4.261.04E-5DIF1Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein
YMR294W-A_d4.181.43E-5YMR294W-A_dDubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents
YHL003C4.161.58E-5LAG1Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
YFL053W3.934.25E-5DAK2Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
YJL154C3.885.13E-5VPS35Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
YOR042W3.836.29E-5CUE5Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YPL236C3.836.42E-5ENV7Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
YGR004W3.807.20E-5PEX31Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p

GO enrichment analysis for SGTC_1059
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0723.34E-8SGTC_2568diosmetin 100.0 μMMicrosource (Natural product library)52816120.0752688
0.0691.29E-7SGTC_5313788-1495 174.0 μMChemDiv (Drug-like library)28966550.214286unfolded protein response
0.0691.71E-7SGTC_3531988-1175 58.3 μMChemDiv (Drug-like library)27883570.1875unfolded protein response
0.0543.83E-5SGTC_21035378138 10.0 μMChembridge (Fragment library)2441420.146667
0.0518.40E-5SGTC_20755212009 200.0 μMChembridge (Fragment library)7808980.0574713
0.0491.46E-4SGTC_10563448-1962 115.0 μMChemDiv (Drug-like library)7155070.105882amide catabolism
0.0455.14E-4SGTC_443clozapine 122.0 μMMiscellaneous28180.0978261NEO1-PIK1
0.0455.58E-4SGTC_32789139605 49.5 μMChembridge (Drug-like library)79588590.1625
0.0420.00120SGTC_22827944268 200.0 μMChembridge (Fragment library)29704140.107143RPP1 & pyrimidine depletion
0.0410.00144SGTC_414piperonyl butoxide 100.0 μMMiscellaneous57940.0930233
0.0410.00167SGTC_32129128307 49.5 μMChembridge (Drug-like library)272435420.129032
0.0410.00168SGTC_31609100216 49.5 μMChembridge (Drug-like library)171910390.147368
0.0410.00178SGTC_25534-methylesculetin 85.1 μMMicrosource (Natural product library)53195020.0617284
0.0400.00187SGTC_60844-0013 19.8 μMChemDiv (Drug-like library)15849000.0786517
0.0400.00221SGTC_12570669-0121 16.0 μMChemDiv (Drug-like library)43078550.091954

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7083448-5400146 μM0.5897443462195ChemDiv (Drug-like library)508.993264.41315
SGTC_13742889-5571141 μM0.4492752853281ChemDiv (Drug-like library)269.724041.89613RSC complex & mRNA processing
SGTC_2946906550271.43 μM0.33333316489101Chembridge (Drug-like library)324.37372.35613
SGTC_3072911641949.47 μM0.32517170162Chembridge (Drug-like library)331.793423.62113ERG2
SGTC_3092911724849.47 μM0.32517141857Chembridge (Drug-like library)346.808063.52923cell wall signaling
SGTC_3227913076849.47 μM0.31325317720899Chembridge (Drug-like library)313.390823.57913
SGTC_2899905388958.44 μM0.3037976470052Chembridge (Drug-like library)276.373963.15922
SGTC_3103912082249.47 μM0.29761917141819Chembridge (Drug-like library)327.374342.9414
SGTC_2837900847645.46 μM0.2771082992805Chembridge (Drug-like library)328.3841.21226
SGTC_21915911152200 μM0.2763162875902Chembridge (Fragment library)279.718861.92403