3448-7350

N-(1-benzofuran-3-yl)benzamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1060
Screen concentration 82.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 570178
SMILES C1=CC=C(C=C1)C(=O)NC2=COC3=CC=CC=C32
Standardized SMILES O=C(Nc1coc2ccccc12)c3ccccc3
Molecular weight 237.2533
ALogP 2.92
H-bond donor count 1
H-bond acceptor count 2
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.04
% growth inhibition (Hom. pool) 11.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 570178
Download HIP data (tab-delimited text)  (excel)
Gene:BDP1(YNL039W)|FD-Score:-3.11|P-value:9.29E-4|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:CDC20(YGL116W)|FD-Score:7.65|P-value:1.01E-14|Clearance:0.82||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.15|P-value:8.22E-4|Clearance:0.01||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CET1(YPL228W)|FD-Score:-3.41|P-value:3.30E-4|Clearance:0||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:CSE1(YGL238W)|FD-Score:4.22|P-value:1.22E-5|Clearance:0.58||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:CSE4(YKL049C)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.11||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:DHR2(YKL078W)|FD-Score:6.83|P-value:4.30E-12|Clearance:0.62||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DYS1(YHR068W)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERG12(YMR208W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.1||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLC7(YER133W)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.09||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HRR25(YPL204W)|FD-Score:-3.81|P-value:7.00E-5|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSH49(YOR319W)|FD-Score:4.8|P-value:8.09E-7|Clearance:0.39||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:NOP56(YLR197W)|FD-Score:5.49|P-value:1.96E-8|Clearance:0.22||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RER2(YBR002C)|FD-Score:3.22|P-value:6.36E-4|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPL18A(YOL120C)|FD-Score:4.41|P-value:5.20E-6|Clearance:0.19||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRP15(YPR143W)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.03||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:SEC13(YLR208W)|FD-Score:11.6|P-value:3.63E-31|Clearance:2.98||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC21(YNL287W)|FD-Score:7.79|P-value:3.38E-15|Clearance:0.14||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC24(YIL109C)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.04||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SEC26(YDR238C)|FD-Score:3.16|P-value:7.90E-4|Clearance:0.01||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC31(YDL195W)|FD-Score:4.91|P-value:4.56E-7|Clearance:0.11||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC24(YMR117C)|FD-Score:6.1|P-value:5.15E-10|Clearance:0.61||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:SPC97(YHR172W)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.01||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP381(YBR152W)|FD-Score:3.37|P-value:3.77E-4|Clearance:0.05||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TAH18(YPR048W)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.01||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TSC13(YDL015C)|FD-Score:-3.13|P-value:8.85E-4|Clearance:0||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:UFE1(YOR075W)|FD-Score:3.14|P-value:8.51E-4|Clearance:0.03||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:USO1(YDL058W)|FD-Score:6.21|P-value:2.71E-10|Clearance:0.1||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:YDL196W(YDL196W_d)|FD-Score:8.58|P-value:4.90E-18|Clearance:0.79||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:5.27|P-value:6.76E-8|Clearance:0.36||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YPT1(YFL038C)|FD-Score:11.7|P-value:7.54E-32|Clearance:2.98||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:BDP1(YNL039W)|FD-Score:-3.11|P-value:9.29E-4|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:CDC20(YGL116W)|FD-Score:7.65|P-value:1.01E-14|Clearance:0.82||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.15|P-value:8.22E-4|Clearance:0.01||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CET1(YPL228W)|FD-Score:-3.41|P-value:3.30E-4|Clearance:0||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:CSE1(YGL238W)|FD-Score:4.22|P-value:1.22E-5|Clearance:0.58||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:CSE4(YKL049C)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.11||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:DHR2(YKL078W)|FD-Score:6.83|P-value:4.30E-12|Clearance:0.62||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:DYS1(YHR068W)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERG12(YMR208W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.1||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:GLC7(YER133W)|FD-Score:3.53|P-value:2.10E-4|Clearance:0.09||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HRR25(YPL204W)|FD-Score:-3.81|P-value:7.00E-5|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSH49(YOR319W)|FD-Score:4.8|P-value:8.09E-7|Clearance:0.39||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:NOP56(YLR197W)|FD-Score:5.49|P-value:1.96E-8|Clearance:0.22||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RER2(YBR002C)|FD-Score:3.22|P-value:6.36E-4|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPL18A(YOL120C)|FD-Score:4.41|P-value:5.20E-6|Clearance:0.19||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RRP15(YPR143W)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.03||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:SEC13(YLR208W)|FD-Score:11.6|P-value:3.63E-31|Clearance:2.98||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC21(YNL287W)|FD-Score:7.79|P-value:3.38E-15|Clearance:0.14||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC24(YIL109C)|FD-Score:3.41|P-value:3.24E-4|Clearance:0.04||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SEC26(YDR238C)|FD-Score:3.16|P-value:7.90E-4|Clearance:0.01||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC31(YDL195W)|FD-Score:4.91|P-value:4.56E-7|Clearance:0.11||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC24(YMR117C)|FD-Score:6.1|P-value:5.15E-10|Clearance:0.61||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:SPC97(YHR172W)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.01||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP381(YBR152W)|FD-Score:3.37|P-value:3.77E-4|Clearance:0.05||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TAH18(YPR048W)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.01||SGD DESC:Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance Gene:TSC13(YDL015C)|FD-Score:-3.13|P-value:8.85E-4|Clearance:0||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:UFE1(YOR075W)|FD-Score:3.14|P-value:8.51E-4|Clearance:0.03||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:USO1(YDL058W)|FD-Score:6.21|P-value:2.71E-10|Clearance:0.1||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:YDL196W(YDL196W_d)|FD-Score:8.58|P-value:4.90E-18|Clearance:0.79||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:5.27|P-value:6.76E-8|Clearance:0.36||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YPT1(YFL038C)|FD-Score:11.7|P-value:7.54E-32|Clearance:2.98||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 570178
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ARL1(YBR164C)|FD-Score:13|P-value:6.31E-39||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:AYR1(YIL124W)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BEM4(YPL161C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BST1(YFL025C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CAF40(YNL288W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CAR2(YLR438W)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CIN4(YMR138W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CMR1(YDL156W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:COA2(YPL189C-A)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG5(YNL051W)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:14.1|P-value:1.16E-45||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:9.82|P-value:4.81E-23||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:14.7|P-value:1.99E-49||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ1(YBR003W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:EFM1(YHL039W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:EMP24(YGL200C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERP2(YAL007C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FAR10(YLR238W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FYV6(YNL133C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAL80(YML051W)|FD-Score:-4.78|P-value:8.61E-7||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:GCG1(YER163C_p)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GEA2(YEL022W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GET2(YER083C)|FD-Score:11.8|P-value:2.26E-32||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GIS4(YML006C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:GTR2(YGR163W)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HSP26(YBR072W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:IRE1(YHR079C)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:IRS4(YKR019C)|FD-Score:22.9|P-value:1.53E-116||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MMS2(YGL087C)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MNL1(YHR204W)|FD-Score:4.95|P-value:3.78E-7||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MNL2(YLR057W)|FD-Score:-4.46|P-value:4.16E-6||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MTC3(YGL226W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NBP2(YDR162C)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NHX1(YDR456W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NOP12(YOL041C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NSR1(YGR159C)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:OCA6(YDR067C_p)|FD-Score:-5.51|P-value:1.75E-8||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PAT1(YCR077C)|FD-Score:5.68|P-value:6.62E-9||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PHO86(YJL117W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PHO87(YCR037C)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PRO1(YDR300C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:RAD10(YML095C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAD2(YGR258C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RGA1(YOR127W)|FD-Score:5.93|P-value:1.53E-9||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIC1(YLR039C)|FD-Score:5.76|P-value:4.29E-9||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPB9(YGL070C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPE1(YJL121C)|FD-Score:-4.15|P-value:1.65E-5||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPN4(YDL020C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:-4.08|P-value:2.25E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:SEY1(YOR165W)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFB3(YHR098C)|FD-Score:-4.38|P-value:5.86E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SLO1(YER180C-A)|FD-Score:-4.31|P-value:8.22E-6||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SMF3(YLR034C)|FD-Score:4|P-value:3.17E-5||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SNF7(YLR025W)|FD-Score:-4.01|P-value:3.09E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOD1(YJR104C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO12(YHR152W)|FD-Score:8.94|P-value:2.00E-19||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SWA2(YDR320C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SYS1(YJL004C)|FD-Score:7.13|P-value:4.97E-13||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THI2(YBR240C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TLG2(YOL018C)|FD-Score:5.78|P-value:3.78E-9||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRS85(YDR108W)|FD-Score:14.3|P-value:1.20E-46||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VBA2(YBR293W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VNX1(YNL321W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS60(YDR486C)|FD-Score:6.39|P-value:8.48E-11||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS61(YDR136C_d)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS72(YDR485C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-4.79|P-value:8.34E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR063C(YBR063C_p)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YDC1(YPL087W)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:-3.75|P-value:8.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-4.34|P-value:7.22E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR278C(YLR278C_p)|FD-Score:-4.86|P-value:5.89E-7||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YMR244W(YMR244W_p)|FD-Score:-5.82|P-value:2.91E-9||SGD DESC:Putative protein of unknown function Gene:YNL140C(YNL140C_p)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNR014W(YNR014W_p)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YNR064C(YNR064C)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL036W(YOL036W_p)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOR283W(YOR283W)|FD-Score:-4.58|P-value:2.38E-6||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR059C(YPR059C_d)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPT6(YLR262C)|FD-Score:6.74|P-value:7.71E-12||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSP1(YHR155W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ZDS1(YMR273C)|FD-Score:5.69|P-value:6.23E-9||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication Gene:ACO1(YLR304C)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ARL1(YBR164C)|FD-Score:13|P-value:6.31E-39||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:AYR1(YIL124W)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BEM4(YPL161C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BST1(YFL025C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CAF40(YNL288W)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CAR2(YLR438W)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CIN4(YMR138W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CMR1(YDL156W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS Gene:COA2(YPL189C-A)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG5(YNL051W)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:14.1|P-value:1.16E-45||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:9.82|P-value:4.81E-23||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:14.7|P-value:1.99E-49||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ1(YBR003W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:EFM1(YHL039W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EFT1(YOR133W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:EMP24(YGL200C)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERP2(YAL007C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FAR10(YLR238W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FYV6(YNL133C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GAL80(YML051W)|FD-Score:-4.78|P-value:8.61E-7||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:GCG1(YER163C_p)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GEA2(YEL022W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GET2(YER083C)|FD-Score:11.8|P-value:2.26E-32||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GIS4(YML006C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:GTR2(YGR163W)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HSP26(YBR072W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:IRE1(YHR079C)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:IRS4(YKR019C)|FD-Score:22.9|P-value:1.53E-116||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MMS2(YGL087C)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MNL1(YHR204W)|FD-Score:4.95|P-value:3.78E-7||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MNL2(YLR057W)|FD-Score:-4.46|P-value:4.16E-6||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MTC3(YGL226W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NBP2(YDR162C)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NHX1(YDR456W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NOP12(YOL041C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NSR1(YGR159C)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:OCA6(YDR067C_p)|FD-Score:-5.51|P-value:1.75E-8||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PAT1(YCR077C)|FD-Score:5.68|P-value:6.62E-9||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PHO86(YJL117W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PHO87(YCR037C)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PRO1(YDR300C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:RAD10(YML095C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAD2(YGR258C)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RGA1(YOR127W)|FD-Score:5.93|P-value:1.53E-9||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIC1(YLR039C)|FD-Score:5.76|P-value:4.29E-9||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPB9(YGL070C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPE1(YJL121C)|FD-Score:-4.15|P-value:1.65E-5||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPN4(YDL020C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS14A(YCR031C)|FD-Score:-4.08|P-value:2.25E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS30A(YLR287C-A)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:SEY1(YOR165W)|FD-Score:-3.91|P-value:4.64E-5||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SFB3(YHR098C)|FD-Score:-4.38|P-value:5.86E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SLO1(YER180C-A)|FD-Score:-4.31|P-value:8.22E-6||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SMF3(YLR034C)|FD-Score:4|P-value:3.17E-5||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SNF7(YLR025W)|FD-Score:-4.01|P-value:3.09E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOD1(YJR104C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO12(YHR152W)|FD-Score:8.94|P-value:2.00E-19||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SWA2(YDR320C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SYS1(YJL004C)|FD-Score:7.13|P-value:4.97E-13||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THI2(YBR240C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TLG2(YOL018C)|FD-Score:5.78|P-value:3.78E-9||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRS85(YDR108W)|FD-Score:14.3|P-value:1.20E-46||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VBA2(YBR293W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VNX1(YNL321W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS60(YDR486C)|FD-Score:6.39|P-value:8.48E-11||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS61(YDR136C_d)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS72(YDR485C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-4.79|P-value:8.34E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR063C(YBR063C_p)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YDC1(YPL087W)|FD-Score:-3.85|P-value:5.91E-5||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:-3.75|P-value:8.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-4.34|P-value:7.22E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR278C(YLR278C_p)|FD-Score:-4.86|P-value:5.89E-7||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YMR244W(YMR244W_p)|FD-Score:-5.82|P-value:2.91E-9||SGD DESC:Putative protein of unknown function Gene:YNL140C(YNL140C_p)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNR014W(YNR014W_p)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YNR064C(YNR064C)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL036W(YOL036W_p)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOR283W(YOR283W)|FD-Score:-4.58|P-value:2.38E-6||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR059C(YPR059C_d)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPT6(YLR262C)|FD-Score:6.74|P-value:7.71E-12||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YSP1(YHR155W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ZDS1(YMR273C)|FD-Score:5.69|P-value:6.23E-9||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFL038C11.707.54E-322.98YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YLR208W11.603.63E-312.98SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YDL196W_d8.584.90E-180.79YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YNL287W7.793.38E-150.14SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YGL116W7.651.01E-140.82CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YKL078W6.834.30E-120.62DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YDL058W6.212.71E-100.10USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YMR117C6.105.15E-100.61SPC24Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YLR197W5.491.96E-80.22NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YGR114C_d5.276.76E-80.36YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YDL195W4.914.56E-70.11SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YOR319W4.808.09E-70.39HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YOL120C4.415.20E-60.19RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YGL238W4.221.22E-50.58CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YKL049C3.641.37E-40.11CSE4Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR019C22.901.53E-116IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YML071C14.701.99E-49COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR108W14.301.20E-46TRS85Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
YNL041C14.101.16E-45COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR164C13.006.31E-39ARL1Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
YER083C11.802.26E-32GET2Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
YGL005C9.824.81E-23COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YHR152W8.942.00E-19SPO12Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis
YJL004C7.134.97E-13SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YLR262C6.747.71E-12YPT6Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
YDR486C6.398.48E-11VPS60Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
YOR127W5.931.53E-9RGA1GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress
YOL018C5.783.78E-9TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YLR039C5.764.29E-9RIC1Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
YMR273C5.696.23E-9ZDS1Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1060
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3384.15E-158SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.107143Golgi
0.3281.21E-147SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.0694444Golgi
0.3002.68E-123SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0484848Golgi
0.2991.74E-122SGTC_389tpck 16.7 μMMiscellaneous4396470.169492
0.2924.02E-116SGTC_560957-0365 83.2 μMChemDiv (Drug-like library)67523600.106667Golgi
0.2811.78E-107SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.186441Golgi
0.2786.21E-105SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.125
0.2756.99E-103SGTC_1751st044839 27.2 μMTimTec (Natural product derivative library)52913260.101449Golgi
0.2721.43E-100SGTC_1669st015249 58.4 μMTimTec (Natural product derivative library)7227930.09375Golgi
0.2706.24E-99SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0684932Golgi
0.2631.32E-93SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.084507Golgi
0.2571.12E-89SGTC_7171532-0246 73.2 μMChemDiv (Drug-like library)95619920.145833Golgi
0.2566.05E-89SGTC_8780828-0289 13.7 μMChemDiv (Drug-like library)7453500.147541
0.2548.67E-88SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.0410959Golgi
0.2504.22E-85SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.103448Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9272425-1185123 μM0.386364730375ChemDiv (Drug-like library)316.353243.13522
SGTC_2923799265458.14 μM0.362985126Chembridge (Drug-like library)358.432983.9612Golgi
SGTC_2274794709446.08 μM0.3555562971028Chembridge (Fragment library)246.2851.40323
SGTC_10154130-452582 μM0.3396231363237ChemDiv (Drug-like library)392.834983.8032360S ribosome export
SGTC_9443370-0224231 μM0.3333334580514ChemDiv (Drug-like library)253.252742.96723
SGTC_9861493-048384 μM0.33333340079ChemDiv (Drug-like library)256.256681.31914
SGTC_20635246145200 μM0.333333711240Chembridge (Fragment library)221.639722.67412
SGTC_3184910875849.47 μM0.333333902885Chembridge (Drug-like library)241.28512.94712
SGTC_5820987-0078156 μM0.3272731625765ChemDiv (Drug-like library)367.419864.38312calcium & mitochondrial duress
SGTC_8261683-5028118 μM0.326531722377ChemDiv (Drug-like library)261.703583.26212