3448-9325

2,2-dimethyl-1,3,5,6-tetrahydrobenzo[a]phenanthridin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1061
Screen concentration 110.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 723482
SMILES CC1(CC2=C(CNC3=C2C4=CC=CC=C4C=C3)C(=O)C1)C
Standardized SMILES CC1(C)CC(=O)C2=C(C1)c3c(NC2)ccc4ccccc34
Molecular weight 277.3603
ALogP 3.66
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.51
% growth inhibition (Hom. pool) 9.1


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 723482
Download HIP data (tab-delimited text)  (excel)
Gene:DHR2(YKL078W)|FD-Score:9.27|P-value:9.26E-21|Clearance:2.53||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:HEM12(YDR047W)|FD-Score:3.28|P-value:5.21E-4|Clearance:0.01||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:LSG1(YGL099W)|FD-Score:4.52|P-value:3.04E-6|Clearance:0.01||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.14||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MED4(YOR174W)|FD-Score:-3.55|P-value:1.91E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NMD3(YHR170W)|FD-Score:3.74|P-value:9.21E-5|Clearance:0.02||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUF2(YOL069W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.09||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:PET9(YBL030C)|FD-Score:-3.22|P-value:6.34E-4|Clearance:0||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:PRP11(YDL043C)|FD-Score:-3.31|P-value:4.72E-4|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RPC37(YKR025W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.26||SGD DESC:RNA polymerase III subunit C37 Gene:RPN7(YPR108W)|FD-Score:-5.21|P-value:9.36E-8|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPS3(YNL178W)|FD-Score:-3.25|P-value:5.87E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP3(YHR065C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.1||SGD DESC:Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity Gene:SEC12(YNR026C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.05||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SEC13(YLR208W)|FD-Score:6.74|P-value:8.05E-12|Clearance:2.21||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SGT1(YOR057W)|FD-Score:4.52|P-value:3.15E-6|Clearance:0.66||SGD DESC:Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress Gene:SMX2(YFL017W-A)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.07||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:TFB3(YDR460W)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TPT1(YOL102C)|FD-Score:3.85|P-value:5.83E-5|Clearance:0.11||SGD DESC:tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide Gene:UTP15(YMR093W)|FD-Score:-4.13|P-value:1.80E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:DHR2(YKL078W)|FD-Score:9.27|P-value:9.26E-21|Clearance:2.53||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:HEM12(YDR047W)|FD-Score:3.28|P-value:5.21E-4|Clearance:0.01||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:LSG1(YGL099W)|FD-Score:4.52|P-value:3.04E-6|Clearance:0.01||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.14||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MED4(YOR174W)|FD-Score:-3.55|P-value:1.91E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NMD3(YHR170W)|FD-Score:3.74|P-value:9.21E-5|Clearance:0.02||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUF2(YOL069W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.09||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:PET9(YBL030C)|FD-Score:-3.22|P-value:6.34E-4|Clearance:0||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:PRP11(YDL043C)|FD-Score:-3.31|P-value:4.72E-4|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RPC37(YKR025W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.26||SGD DESC:RNA polymerase III subunit C37 Gene:RPN7(YPR108W)|FD-Score:-5.21|P-value:9.36E-8|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPS3(YNL178W)|FD-Score:-3.25|P-value:5.87E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP3(YHR065C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.1||SGD DESC:Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity Gene:SEC12(YNR026C)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.05||SGD DESC:Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER Gene:SEC13(YLR208W)|FD-Score:6.74|P-value:8.05E-12|Clearance:2.21||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SGT1(YOR057W)|FD-Score:4.52|P-value:3.15E-6|Clearance:0.66||SGD DESC:Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress Gene:SMX2(YFL017W-A)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.07||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:TFB3(YDR460W)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TPT1(YOL102C)|FD-Score:3.85|P-value:5.83E-5|Clearance:0.11||SGD DESC:tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide Gene:UTP15(YMR093W)|FD-Score:-4.13|P-value:1.80E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 723482
Download HOP data (tab-delimited text)  (excel)
Gene:ADE12(YNL220W)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:ALY1(YKR021W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:4.27|P-value:9.63E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATO2(YNR002C)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:COQ8(YGL119W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:4.97|P-value:3.28E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DFG16(YOR030W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:EFM1(YHL039W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EUG1(YDR518W)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:GDS1(YOR355W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLR1(YPL091W)|FD-Score:-4.44|P-value:4.49E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:HIR3(YJR140C)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HPA3(YEL066W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:IDH2(YOR136W)|FD-Score:7.33|P-value:1.19E-13||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:INM1(YHR046C)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IOC3(YFR013W)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRE1(YHR079C)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LEO1(YOR123C)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:MGT1(YDL200C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MRPL27(YBR282W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL37(YBR268W)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS5(YBR251W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NGL1(YOL042W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NHP6B(YBR089C-A)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication Gene:OPT1(YJL212C)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family Gene:PCH2(YBR186W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation Gene:PNG1(YPL096W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:QCR9(YGR183C)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RGD1(YBR260C)|FD-Score:4.9|P-value:4.90E-7||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RIC1(YLR039C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL20A(YMR242C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RRG9(YNL213C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SED4(YCR067C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SET5(YHR207C)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SKN7(YHR206W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNF7(YLR025W)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPC1(YJR010C-A)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SRB8(YCR081W)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSD1(YDR293C)|FD-Score:4.37|P-value:6.31E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSK2(YNR031C)|FD-Score:4|P-value:3.14E-5||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SSN3(YPL042C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STE23(YLR389C)|FD-Score:6.86|P-value:3.37E-12||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STE50(YCL032W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SWI3(YJL176C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TDP1(YBR223C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TIM11(YDR322C-A)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TRM11(YOL124C)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TRP2(YER090W)|FD-Score:5.27|P-value:6.92E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS33(YOR115C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UME6(YDR207C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:URA5(YML106W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:VHS1(YDR247W)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:VMA7(YGR020C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS45(YGL095C)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:WWM1(YFL010C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:XYL2(YLR070C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBR197C(YBR197C_p)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YHL017W(YHL017W_p)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIA6(YIL006W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL058W(YIL058W_d)|FD-Score:4.9|P-value:4.91E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL152W(YJL152W_d)|FD-Score:5.54|P-value:1.52E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLF2(YHL014C)|FD-Score:4.62|P-value:1.89E-6||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL006W-A(YLL006W-A_p)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR063W(YLR063W_p)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR416C(YLR416C_d)|FD-Score:4.98|P-value:3.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:-4.52|P-value:3.05E-6||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YOR072W(YOR072W_d)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YSR3(YKR053C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:ADE12(YNL220W)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:ALY1(YKR021W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:4.27|P-value:9.63E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATO2(YNR002C)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:COQ8(YGL119W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:DAL81(YIR023W)|FD-Score:4.97|P-value:3.28E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DFG16(YOR030W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:EFM1(YHL039W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EUG1(YDR518W)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:GDS1(YOR355W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GLR1(YPL091W)|FD-Score:-4.44|P-value:4.49E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:HIR3(YJR140C)|FD-Score:5.13|P-value:1.43E-7||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HPA3(YEL066W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:IDH2(YOR136W)|FD-Score:7.33|P-value:1.19E-13||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:INM1(YHR046C)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IOC3(YFR013W)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRE1(YHR079C)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LEO1(YOR123C)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:MGT1(YDL200C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MRPL27(YBR282W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL37(YBR268W)|FD-Score:-4.26|P-value:1.03E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS5(YBR251W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NGL1(YOL042W)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NHP6B(YBR089C-A)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication Gene:OPT1(YJL212C)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family Gene:PCH2(YBR186W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation Gene:PNG1(YPL096W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:QCR9(YGR183C)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RGD1(YBR260C)|FD-Score:4.9|P-value:4.90E-7||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RIC1(YLR039C)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL20A(YMR242C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RRG9(YNL213C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SED4(YCR067C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SET5(YHR207C)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SKN7(YHR206W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLS1(YLR139C)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNF7(YLR025W)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPC1(YJR010C-A)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SRB8(YCR081W)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSD1(YDR293C)|FD-Score:4.37|P-value:6.31E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSK2(YNR031C)|FD-Score:4|P-value:3.14E-5||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SSN3(YPL042C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STE23(YLR389C)|FD-Score:6.86|P-value:3.37E-12||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STE50(YCL032W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SWI3(YJL176C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TDP1(YBR223C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TIM11(YDR322C-A)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TRM11(YOL124C)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TRP2(YER090W)|FD-Score:5.27|P-value:6.92E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.32|P-value:7.87E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRS33(YOR115C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UME6(YDR207C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:URA5(YML106W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:VHS1(YDR247W)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:VMA7(YGR020C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS45(YGL095C)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:WWM1(YFL010C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1 Gene:XYL2(YLR070C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YBR197C(YBR197C_p)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YHL017W(YHL017W_p)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication Gene:YIA6(YIL006W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL058W(YIL058W_d)|FD-Score:4.9|P-value:4.91E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL152W(YJL152W_d)|FD-Score:5.54|P-value:1.52E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLF2(YHL014C)|FD-Score:4.62|P-value:1.89E-6||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL006W-A(YLL006W-A_p)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR063W(YLR063W_p)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR416C(YLR416C_d)|FD-Score:4.98|P-value:3.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:-4.52|P-value:3.05E-6||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YOR072W(YOR072W_d)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YSR3(YKR053C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL078W9.279.26E-212.53DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YLR208W6.748.05E-122.21SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YGL099W4.523.04E-60.01LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YOR057W4.523.15E-60.66SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
YOL102C3.855.83E-50.11TPT1tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
YHR170W3.749.21E-50.02NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YHR065C3.721.00E-40.10RRP3Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
YNR026C3.621.45E-40.05SEC12Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER
YDR460W3.581.74E-40.01TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YER112W3.571.81E-40.14LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YFL017W-A3.433.01E-40.07SMX2Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G
YOL069W3.363.84E-40.09NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YDR047W3.285.21E-40.01HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YKR025W3.275.37E-40.26RPC37RNA polymerase III subunit C37
YDR196C3.010.001290.14CAB5Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR136W7.331.19E-13IDH2Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
YLR389C6.863.37E-12STE23Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family
YJL152W_d5.541.52E-8YJL152W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL095C5.422.94E-8VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YER090W5.276.92E-8TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YJR140C5.131.43E-7HIR3Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
YOR123C5.091.80E-7LEO1Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
YNR002C5.062.12E-7ATO2Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication
YLR416C_d4.983.23E-7YLR416C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YIR023W4.973.28E-7DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YBR260C4.904.90E-7RGD1GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
YIL058W_d4.904.91E-7YIL058W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHL014C4.621.89E-6YLF2Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YHR079C4.444.52E-6IRE1Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
YFR013W4.395.74E-6IOC3Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1061
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1548.44E-33SGTC_1710st031758 61.5 μMTimTec (Natural product derivative library)53943410.0897436
0.1475.35E-30SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.117647
0.1441.32E-28SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.117647
0.1381.56E-26SGTC_14163966-0321 25.3 μMChemDiv (Drug-like library)5825300.137931
0.1382.47E-26SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.060241
0.1376.11E-26SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0724638
0.1331.10E-24SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.109375
0.1331.20E-24SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.114754
0.1314.35E-24SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.129032
0.1292.12E-23SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0581395
0.1261.84E-22SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.0985916
0.1254.11E-22SGTC_24725377652 49.4 μMMiscellaneous16109030.140625TRP & mitochondrial translation
0.1255.39E-22SGTC_7234182-0001 115.0 μMChemDiv (Drug-like library)7448210.0909091
0.1249.81E-22SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.20339
0.1241.32E-21SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0895522

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_23799035213200 μM0.321429646551Chembridge (Fragment library)240.300342.32213
SGTC_5491661-1316519 μM0.3194442857282ChemDiv (Drug-like library)385.455024.90324
SGTC_2435hr heat shock (37°C) + splitomicin1 μM0.2692315269Miscellaneous198.21732.83102heat shock/prefoldin
SGTC_424splitomicin100 mM0.2692315269Miscellaneous198.21732.83102
SGTC_11283448-9350235 μM0.257143619386ChemDiv (Drug-like library)340.41774.57613
SGTC_22226885208172.14 μM0.254545609514Chembridge (Fragment library)233.26311.43313
SGTC_1859st05599820 μM0.254237676301TimTec (Natural product derivative library)288.296783.80213
SGTC_1842st05536261.2 μM0.241935676032TimTec (Natural product derivative library)304.296183.5624
SGTC_3244913448949.47 μM0.22857116584271Chembridge (Drug-like library)340.806783.51604
SGTC_1070358-00222.69 μM0.22449521106ChemDiv (Drug-like library)350.453965.87124SWF1 & branched chain AA biosynthesis