ketoconazole FDA approved compound

1-[4-[4-[[(2R,4S)-2-(2,4-dichlorophenyl)-2-(imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy]phenyl]piperazin-1-yl]ethanone


Established Yeast Drug Target : ERG11

Ketoconazole is an imidazole antifungal; acts by inhibiting lanosterol 14α-demethylase preventing the conversion of lanosterol to ergosterol, an essential component of the fungal cytoplasmic membrane.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1066
Screen concentration 628.0 nM
Source NIH Clinical Collection
PubChem CID 456201
SMILES CC(=O)N1CCN(CC1)C2=CC=C(C=C2)OCC3COC(O3)(CN4C=CN=C4)C5=C(C=C(C=C5)Cl)Cl
Standardized SMILES CC(=O)N1CCN(CC1)c2ccc(OCC3COC(Cn4ccnc4)(O3)c5ccc(Cl)cc5Cl)cc2
Molecular weight 531.4309
ALogP 3.61
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -0.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 456201
Download HIP data (tab-delimited text)  (excel)
Gene:EXO84(YBR102C)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.37||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GPN2(YOR262W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.37||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:MRD1(YPR112C)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.37||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NRD1(YNL251C)|FD-Score:3.14|P-value:8.59E-4|Clearance:0.05||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:PRP6(YBR055C)|FD-Score:-3.19|P-value:7.02E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RRP45(YDR280W)|FD-Score:3.5|P-value:2.30E-4|Clearance:0.37||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC14(YMR079W)|FD-Score:3.81|P-value:7.06E-5|Clearance:0.37||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:UTP14(YML093W)|FD-Score:-3.61|P-value:1.52E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL016C(YDL016C_d)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YDL163W(YDL163W_d)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.37||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:EXO84(YBR102C)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.37||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GPN2(YOR262W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.37||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:MRD1(YPR112C)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.37||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:NRD1(YNL251C)|FD-Score:3.14|P-value:8.59E-4|Clearance:0.05||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:PRP6(YBR055C)|FD-Score:-3.19|P-value:7.02E-4|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RRP45(YDR280W)|FD-Score:3.5|P-value:2.30E-4|Clearance:0.37||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC14(YMR079W)|FD-Score:3.81|P-value:7.06E-5|Clearance:0.37||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:UTP14(YML093W)|FD-Score:-3.61|P-value:1.52E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL016C(YDL016C_d)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YDL163W(YDL163W_d)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.37||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 456201
Download HOP data (tab-delimited text)  (excel)
Gene:ADY3(YDL239C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:AGA2(YGL032C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AHC2(YCR082W)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM18(YHR198C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD6(YPL061W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:5.21|P-value:9.58E-8||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APD1(YBR151W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:APS1(YLR170C)|FD-Score:-6.58|P-value:2.33E-11||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARC18(YLR370C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARR2(YPR200C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:BIT61(YJL058C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD31(YCR063W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CAT8(YMR280C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CLB2(YPR119W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG7(YGL005C)|FD-Score:-5.2|P-value:1.01E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSF1(YLR087C)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTF4(YPR135W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DCI1(YOR180C)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DSS1(YMR287C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM4(YKR076W)|FD-Score:4.78|P-value:8.89E-7||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:FYV10(YIL097W)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:FZO1(YBR179C)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:HMG1(YML075C)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOG1(YLR113W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HXK2(YGL253W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:ICS2(YBR157C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:ILM1(YJR118C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:LIA1(YJR070C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MSD1(YPL104W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSF1(YPR047W)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSP1(YGR028W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase Gene:NIF3(YGL221C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NPT1(YOR209C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OMS1(YDR316W)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:OPI3(YJR073C)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OXA1(YER154W)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PDA1(YER178W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PDR1(YGL013C)|FD-Score:7.74|P-value:5.13E-15||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR16(YNL231C)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PIF1(YML061C)|FD-Score:4.29|P-value:9.07E-6||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIR3(YKL163W)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:RBD2(YPL246C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:RDS1(YCR106W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REV3(YPL167C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RMD8(YFR048W)|FD-Score:-3.72|P-value:9.98E-5||SGD DESC:Cytosolic protein required for sporulation Gene:SAS3(YBL052C)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SEA4(YBL104C)|FD-Score:5.44|P-value:2.61E-8||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SLA1(YBL007C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLX9(YGR081C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SMF3(YLR034C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:UTR1(YJR049C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAC14(YLR386W)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS25(YJR102C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS36(YLR417W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS4(YPR173C)|FD-Score:5.39|P-value:3.61E-8||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YDL118W(YDL118W_p)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR340W(YDR340W_d)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Putative protein of unknown function Gene:YDR476C(YDR476C_p)|FD-Score:4.31|P-value:8.08E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YFL012W(YFL012W_p)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YHR097C(YHR097C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKR073C(YKR073C_d)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR283W(YLR283W_p)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YML003W(YML003W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPS1(YLR120C)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:ZIP2(YGL249W)|FD-Score:4.81|P-value:7.56E-7||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADY3(YDL239C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:AGA2(YGL032C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AHC2(YCR082W)|FD-Score:3.9|P-value:4.73E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:AIM18(YHR198C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD6(YPL061W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALF1(YNL148C)|FD-Score:5.21|P-value:9.58E-8||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APD1(YBR151W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:APS1(YLR170C)|FD-Score:-6.58|P-value:2.33E-11||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARC18(YLR370C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARR2(YPR200C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:BIT61(YJL058C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD31(YCR063W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CAT8(YMR280C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CLB2(YPR119W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COG7(YGL005C)|FD-Score:-5.2|P-value:1.01E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSF1(YLR087C)|FD-Score:4.44|P-value:4.58E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTF4(YPR135W)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DCI1(YOR180C)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DSS1(YMR287C)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM4(YKR076W)|FD-Score:4.78|P-value:8.89E-7||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:FYV10(YIL097W)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:FZO1(YBR179C)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:HMG1(YML075C)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOG1(YLR113W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HXK2(YGL253W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:ICS2(YBR157C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:ILM1(YJR118C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:LIA1(YJR070C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MSD1(YPL104W)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSF1(YPR047W)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSP1(YGR028W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase Gene:NIF3(YGL221C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NPT1(YOR209C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OMS1(YDR316W)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:OPI3(YJR073C)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OXA1(YER154W)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PDA1(YER178W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PDR1(YGL013C)|FD-Score:7.74|P-value:5.13E-15||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR16(YNL231C)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PIF1(YML061C)|FD-Score:4.29|P-value:9.07E-6||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIR3(YKL163W)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:RBD2(YPL246C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:RDS1(YCR106W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REV3(YPL167C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RMD8(YFR048W)|FD-Score:-3.72|P-value:9.98E-5||SGD DESC:Cytosolic protein required for sporulation Gene:SAS3(YBL052C)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SEA4(YBL104C)|FD-Score:5.44|P-value:2.61E-8||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SLA1(YBL007C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLX9(YGR081C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SMF3(YLR034C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:UTR1(YJR049C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VAC14(YLR386W)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS25(YJR102C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS36(YLR417W)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS4(YPR173C)|FD-Score:5.39|P-value:3.61E-8||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YDL118W(YDL118W_p)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR340W(YDR340W_d)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Putative protein of unknown function Gene:YDR476C(YDR476C_p)|FD-Score:4.31|P-value:8.08E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YFL012W(YFL012W_p)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YHR097C(YHR097C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKR073C(YKR073C_d)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR283W(YLR283W_p)|FD-Score:-3.32|P-value:4.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YML003W(YML003W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function Gene:YPK1(YKL126W)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPS1(YLR120C)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:ZIP2(YGL249W)|FD-Score:4.81|P-value:7.56E-7||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR102C4.201.33E-50.37EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YDL163W_d4.052.61E-50.37YDL163W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
YMR079W3.817.06E-50.37SEC14Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
YOR262W3.601.58E-40.37GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YPR112C3.591.63E-40.37MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YDR280W3.502.30E-40.37RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YNL251C3.148.59E-40.05NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YEL002C3.080.001030.06WBP1Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
YNL172W3.020.001280.05APC1Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
YOR341W2.970.001500.01RPA190RNA polymerase I largest subunit A190
YDR527W2.960.001530.01RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YIR022W2.950.001600.12SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YOR057W2.830.002330.04SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
YGR280C2.790.002610.06PXR1Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
YJL019W2.740.003110.01MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C7.745.13E-15PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YBL104C5.442.61E-8SEA4Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites
YPR173C5.393.61E-8VPS4AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
YNL148C5.219.58E-8ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YJR073C5.181.12E-7OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YLR386W5.071.99E-7VAC14Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p
YNL231C4.963.46E-7PDR16Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress
YGL249W4.817.56E-7ZIP2Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
YKR076W4.788.89E-7ECM4Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YDR316W4.444.41E-6OMS1Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations
YLR087C4.444.58E-6CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YDR476C_p4.318.08E-6YDR476C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
YML061C4.299.07E-6PIF1DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
YDL118W_p4.171.50E-5YDL118W_pDubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
YGR028W4.112.00E-5MSP1Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase

GO enrichment analysis for SGTC_1066
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1843.64E-46SGTC_2647atorvastatin 60.2 μMMiscellaneous608230.0743802azole & statin
0.1764.83E-42SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.0743802azole & statin
0.1533.04E-32SGTC_1788fluconazole 20.0 μMMiscellaneous33650.09375azole & statin
0.1511.92E-31SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.12069PDR1
0.1411.10E-27SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.104348PDR1
0.1411.53E-27SGTC_2765myclobutanil 7.7 μMMiscellaneous63360.131313azole & statin
0.1361.06E-25SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.104651PDR1
0.1314.91E-24SGTC_1084voriconazole 435.0 nMNIH Clinical Collection716160.0917431azole & statin
0.1292.90E-23SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.0754717ERG2
0.1254.07E-22SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0348837
0.1241.12E-21SGTC_676falcarinol 140.0 μMMiscellaneous33220.0196078
0.1241.47E-21SGTC_1097itavastatin 7.2 μMNIH Clinical Collection52824510.0909091azole & statin
0.1224.58E-21SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0792079PDR1
0.1219.20E-21SGTC_1067clotrimazole 1.4 μMNIH Clinical Collection28120.159091azole & statin
0.1163.51E-19SGTC_10553448-0672 86.0 μMChemDiv (Drug-like library)7483780.11828

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_217ketoconazole989 nM1456201Miscellaneous531.430923.6106ergosterol depletion effects on membrane
SGTC_1076itraconazole331 nM0.5408163793Miscellaneous705.633426.43409
SGTC_1072oxiconazole14.9 nM0.27777847316NIH Clinical Collection492.140164.26816ergosterol depletion effects on membrane
SGTC_252miconazole1.31 nM0.2727274189Miscellaneous416.128565.64502ergosterol depletion effects on membrane
SGTC_57miconazole162.5 nM0.2727274189ChemDiv (Drug-like library)416.128565.64502ergosterol depletion effects on membrane
SGTC_3214534-389065.3 μM0.268817699746ChemDiv (Drug-like library)345.82333.24704RPP1 & pyrimidine depletion
SGTC_2861902459519.48 μM0.26041716482810Chembridge (Drug-like library)401.886562.9914
SGTC_3464513-00427.16 μM0.2553192827372ChemDiv (Drug-like library)352.38383.07105azole & statin
SGTC_2778tioconazole65.09 nM0.2553195482Miscellaneous387.711224.43703ergosterol depletion effects on membrane
SGTC_3146909560949.47 μM0.25531917173999Chembridge (Drug-like library)380.268363.9104
SGTC_2777climbazole1.54 μM0.25287437907Miscellaneous292.760683.76603iron homeostasis