oxiconazole FDA approved compound

1-(2,4-dichlorophenyl)-N-[(2,4-dichlorophenyl)methoxy]-2-imidazol-1-ylethanimine; nitric acid


Established Yeast Drug Target : ERG11

Oxiconazole is a topically used antifungal that acts by inhibiting lanosterol 14α-demethylase preventing the conversion of lanosterol to ergosterol, an essential component of the fungal cytoplasmic membrane.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1072
Screen concentration 14.9 nM
Source NIH Clinical Collection
PubChem CID 47316
SMILES C1=CC(=C(C=C1Cl)Cl)CON=C(CN2C=CN=C2)C3=C(C=C(C=C3)Cl)Cl.[N+](=O)(O)[O-]
Standardized SMILES Clc1ccc(CON=C(Cn2ccnc2)c3ccc(Cl)cc3Cl)c(Cl)c1
Molecular weight 492.1402
ALogP 4.27
H-bond donor count 1
H-bond acceptor count 6
Response signature ergosterol depletion effects on membrane

Pool Growth Kinetics
% growth inhibition (Het. pool) 39.08
% growth inhibition (Hom. pool) 22.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 47316
Download HIP data (tab-delimited text)  (excel)
Gene:CDC19(YAL038W)|FD-Score:11.4|P-value:1.39E-30|Clearance:0.17||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC20(YGL116W)|FD-Score:8.01|P-value:5.88E-16|Clearance:0.53||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC28(YBR160W)|FD-Score:3.35|P-value:4.03E-4|Clearance:0.09||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:DYS1(YHR068W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.03||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERG11(YHR007C)|FD-Score:6.1|P-value:5.37E-10|Clearance:0.39||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:-3.94|P-value:4.13E-5|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:EXO84(YBR102C)|FD-Score:5.7|P-value:5.87E-9|Clearance:0.06||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:FAP7(YDL166C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.02||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FCP1(YMR277W)|FD-Score:6.82|P-value:4.57E-12|Clearance:0.72||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLC7(YER133W)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI2(YPL076W)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.11||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:HEM4(YOR278W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.02||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HSH49(YOR319W)|FD-Score:-3.18|P-value:7.36E-4|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MED6(YHR058C)|FD-Score:3.76|P-value:8.55E-5|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:NFS1(YCL017C)|FD-Score:3.72|P-value:9.79E-5|Clearance:0.08||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:PUP1(YOR157C)|FD-Score:-3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RET3(YPL010W)|FD-Score:-3.4|P-value:3.31E-4|Clearance:0||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPA43(YOR340C)|FD-Score:-5.69|P-value:6.42E-9|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB8(YOR224C)|FD-Score:-3.75|P-value:8.81E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN11(YFR004W)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPS20(YHL015W)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPT1(YKL145W)|FD-Score:-3.47|P-value:2.56E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RRP7(YCL031C)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.28||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:RRP9(YPR137W)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC6(YCR052W)|FD-Score:-4.2|P-value:1.32E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC13(YLR208W)|FD-Score:9.44|P-value:1.92E-21|Clearance:0.84||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC15(YGL233W)|FD-Score:8.6|P-value:4.02E-18|Clearance:0.59||SGD DESC:Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Gene:SEC23(YPR181C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.05||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC3(YER008C)|FD-Score:7.48|P-value:3.71E-14|Clearance:0.66||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SEC31(YDL195W)|FD-Score:3.22|P-value:6.37E-4|Clearance:0.12||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC4(YFL005W)|FD-Score:3.73|P-value:9.53E-5|Clearance:0.01||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SEC5(YDR166C)|FD-Score:4.42|P-value:4.99E-6|Clearance:0.11||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SEC6(YIL068C)|FD-Score:5.5|P-value:1.94E-8|Clearance:0.16||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SEC8(YPR055W)|FD-Score:5.34|P-value:4.68E-8|Clearance:0.23||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SPC97(YHR172W)|FD-Score:3.25|P-value:5.86E-4|Clearance:0.02||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP382(YLR424W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.01||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SUB2(YDL084W)|FD-Score:4.31|P-value:8.30E-6|Clearance:0.05||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TFA2(YKR062W)|FD-Score:3.96|P-value:3.78E-5|Clearance:0.2||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TRS120(YDR407C)|FD-Score:4.26|P-value:1.04E-5|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS130(YMR218C)|FD-Score:5.65|P-value:8.20E-9|Clearance:0.15||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:UBC1(YDR177W)|FD-Score:3.1|P-value:9.70E-4|Clearance:0.02||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP22(YGR090W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.08||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:UTP5(YDR398W)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL196W(YDL196W_d)|FD-Score:17.9|P-value:7.71E-72|Clearance:6.45||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR413C(YDR413C_d)|FD-Score:5.11|P-value:1.59E-7|Clearance:0.69||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YGR114C(YGR114C_d)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL195C(YJL195C_d)|FD-Score:11.3|P-value:9.74E-30|Clearance:1.83||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTH1(YPR107C)|FD-Score:-4.24|P-value:1.11E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:CDC19(YAL038W)|FD-Score:11.4|P-value:1.39E-30|Clearance:0.17||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC20(YGL116W)|FD-Score:8.01|P-value:5.88E-16|Clearance:0.53||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC28(YBR160W)|FD-Score:3.35|P-value:4.03E-4|Clearance:0.09||SGD DESC:Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress Gene:DYS1(YHR068W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.03||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERG11(YHR007C)|FD-Score:6.1|P-value:5.37E-10|Clearance:0.39||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG25(YGR060W)|FD-Score:-3.94|P-value:4.13E-5|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:EXO84(YBR102C)|FD-Score:5.7|P-value:5.87E-9|Clearance:0.06||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:FAP7(YDL166C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.02||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FCP1(YMR277W)|FD-Score:6.82|P-value:4.57E-12|Clearance:0.72||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLC7(YER133W)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.01||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI2(YPL076W)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.11||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:HEM4(YOR278W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.02||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HSH49(YOR319W)|FD-Score:-3.18|P-value:7.36E-4|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MED6(YHR058C)|FD-Score:3.76|P-value:8.55E-5|Clearance:0.03||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:NFS1(YCL017C)|FD-Score:3.72|P-value:9.79E-5|Clearance:0.08||SGD DESC:Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria Gene:PUP1(YOR157C)|FD-Score:-3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RET3(YPL010W)|FD-Score:-3.4|P-value:3.31E-4|Clearance:0||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPA43(YOR340C)|FD-Score:-5.69|P-value:6.42E-9|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB8(YOR224C)|FD-Score:-3.75|P-value:8.81E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN11(YFR004W)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPS20(YHL015W)|FD-Score:-3.21|P-value:6.71E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPT1(YKL145W)|FD-Score:-3.47|P-value:2.56E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RRP7(YCL031C)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.28||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:RRP9(YPR137W)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC6(YCR052W)|FD-Score:-4.2|P-value:1.32E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC13(YLR208W)|FD-Score:9.44|P-value:1.92E-21|Clearance:0.84||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC15(YGL233W)|FD-Score:8.6|P-value:4.02E-18|Clearance:0.59||SGD DESC:Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Gene:SEC23(YPR181C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.05||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SEC3(YER008C)|FD-Score:7.48|P-value:3.71E-14|Clearance:0.66||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SEC31(YDL195W)|FD-Score:3.22|P-value:6.37E-4|Clearance:0.12||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC4(YFL005W)|FD-Score:3.73|P-value:9.53E-5|Clearance:0.01||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SEC5(YDR166C)|FD-Score:4.42|P-value:4.99E-6|Clearance:0.11||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SEC6(YIL068C)|FD-Score:5.5|P-value:1.94E-8|Clearance:0.16||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SEC8(YPR055W)|FD-Score:5.34|P-value:4.68E-8|Clearance:0.23||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SPC97(YHR172W)|FD-Score:3.25|P-value:5.86E-4|Clearance:0.02||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SPP382(YLR424W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.01||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SUB2(YDL084W)|FD-Score:4.31|P-value:8.30E-6|Clearance:0.05||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TFA2(YKR062W)|FD-Score:3.96|P-value:3.78E-5|Clearance:0.2||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TRS120(YDR407C)|FD-Score:4.26|P-value:1.04E-5|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS130(YMR218C)|FD-Score:5.65|P-value:8.20E-9|Clearance:0.15||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:UBC1(YDR177W)|FD-Score:3.1|P-value:9.70E-4|Clearance:0.02||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP22(YGR090W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.08||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:UTP5(YDR398W)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL196W(YDL196W_d)|FD-Score:17.9|P-value:7.71E-72|Clearance:6.45||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR413C(YDR413C_d)|FD-Score:5.11|P-value:1.59E-7|Clearance:0.69||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YGR114C(YGR114C_d)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YJL195C(YJL195C_d)|FD-Score:11.3|P-value:9.74E-30|Clearance:1.83||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTH1(YPR107C)|FD-Score:-4.24|P-value:1.11E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 47316
Download HOP data (tab-delimited text)  (excel)
Gene:AEP2(YMR282C)|FD-Score:-4.95|P-value:3.80E-7||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AFT1(YGL071W)|FD-Score:-4.37|P-value:6.19E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM18(YHR198C)|FD-Score:6.63|P-value:1.70E-11||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM24(YJR080C)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:APL2(YKL135C)|FD-Score:6.46|P-value:5.30E-11||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:APM2(YHL019C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:APM3(YBR288C)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:APS1(YLR170C)|FD-Score:11.6|P-value:2.83E-31||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARG80(YMR042W)|FD-Score:4.27|P-value:9.84E-6||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:ARL3(YPL051W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:6.63|P-value:1.72E-11||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:5.18|P-value:1.08E-7||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATP5(YDR298C)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BCK1(YJL095W)|FD-Score:4.43|P-value:4.78E-6||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BPH1(YCR032W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BRE1(YDL074C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRE2(YLR015W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BTS1(YPL069C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCM1(YGR150C)|FD-Score:-4.46|P-value:4.05E-6||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CKB2(YOR039W)|FD-Score:4.49|P-value:3.53E-6||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNB1(YKL190W)|FD-Score:10.8|P-value:1.60E-27||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COA6(YMR244C-A_p)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:-10|P-value:5.44E-24||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-5.45|P-value:2.52E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX7(YMR256C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYS4(YGR155W)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DFG16(YOR030W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DGR2(YKL121W_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIG1(YPL049C)|FD-Score:4.84|P-value:6.43E-7||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DPP1(YDR284C)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DRS2(YAL026C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSS1(YMR287C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EAR1(YMR171C)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:FMC1(YIL098C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GCN2(YDR283C)|FD-Score:10.9|P-value:5.41E-28||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:6.11|P-value:5.13E-10||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.63|P-value:9.15E-9||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCS1(YDL226C)|FD-Score:9.65|P-value:2.48E-22||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GYP1(YOR070C)|FD-Score:7.74|P-value:4.84E-15||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HIM1(YDR317W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HMG1(YML075C)|FD-Score:5.56|P-value:1.32E-8||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOG1(YLR113W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HRD1(YOL013C)|FD-Score:4.54|P-value:2.87E-6||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:ICE2(YIL090W)|FD-Score:5.5|P-value:1.93E-8||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IMP2'(YIL154C)|FD-Score:11.3|P-value:5.15E-30||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISM1(YPL040C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:IST2(YBR086C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:LAT1(YNL071W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LIP2(YLR239C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAL11(YGR289C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MEF2(YJL102W)|FD-Score:-4.02|P-value:2.91E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MET18(YIL128W)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MID2(YLR332W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRE11(YMR224C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSH3(YCR092C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTG1(YMR097C)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NHP6B(YBR089C-A)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication Gene:PCS60(YBR222C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PFS1(YHR185C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHO84(YML123C)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:POA1(YBR022W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:POR1(YNL055C)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPL2A(YFR031C-A)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:5.36|P-value:4.21E-8||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:5.22|P-value:8.71E-8||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC3(YHR087W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTC6(YPL183W-A)|FD-Score:-3.95|P-value:3.87E-5||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC6(YDR129C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SGF73(YGL066W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIF2(YBR103W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SLG1(YOR008C)|FD-Score:-4.85|P-value:6.07E-7||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:7.85|P-value:2.12E-15||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT3(YDR392W)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:5.37|P-value:3.85E-8||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SSK1(YLR006C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:STE13(YOR219C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:SYS1(YJL004C)|FD-Score:-4.45|P-value:4.33E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TRP1(YDR007W)|FD-Score:6.66|P-value:1.35E-11||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:10.1|P-value:1.79E-24||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.1|P-value:6.15E-13||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.71|P-value:1.51E-18||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP18(YMR071C)|FD-Score:9.3|P-value:6.83E-21||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBR2(YLR024C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:UPC2(YDR213W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:URA4(YLR420W)|FD-Score:6.86|P-value:3.55E-12||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:URE2(YNL229C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VMA16(YHR026W)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS61(YDR136C_d)|FD-Score:-3.75|P-value:8.93E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:WSC2(YNL283C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBL010C(YBL010C_p)|FD-Score:9.38|P-value:3.23E-21||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YDL118W(YDL118W_p)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:5.82|P-value:3.02E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR154C(YDR154C_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YFL034W(YFL034W_p)|FD-Score:11.3|P-value:4.15E-30||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YGL108C(YGL108C_p)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGL176C(YGL176C_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YJR079W(YJR079W_p)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YKL069W(YKL069W)|FD-Score:4.51|P-value:3.22E-6||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL136W(YKL136W_d)|FD-Score:4.44|P-value:4.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR169W(YLR169W_d)|FD-Score:5.26|P-value:7.14E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR171W(YLR171W_d)|FD-Score:6.8|P-value:5.13E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNG1(YOR064C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL182C(YPL182C_d)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR027C(YPR027C_p)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Putative protein of unknown function Gene:YPT31(YER031C)|FD-Score:9.46|P-value:1.50E-21||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YSR3(YKR053C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:AEP2(YMR282C)|FD-Score:-4.95|P-value:3.80E-7||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AFT1(YGL071W)|FD-Score:-4.37|P-value:6.19E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM18(YHR198C)|FD-Score:6.63|P-value:1.70E-11||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM24(YJR080C)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:APL2(YKL135C)|FD-Score:6.46|P-value:5.30E-11||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:APM2(YHL019C)|FD-Score:4|P-value:3.13E-5||SGD DESC:Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport Gene:APM3(YBR288C)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:APS1(YLR170C)|FD-Score:11.6|P-value:2.83E-31||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARG80(YMR042W)|FD-Score:4.27|P-value:9.84E-6||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:ARL3(YPL051W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:6.63|P-value:1.72E-11||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:5.18|P-value:1.08E-7||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATP5(YDR298C)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BCK1(YJL095W)|FD-Score:4.43|P-value:4.78E-6||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BPH1(YCR032W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BRE1(YDL074C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRE2(YLR015W)|FD-Score:4.44|P-value:4.51E-6||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BTS1(YPL069C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCM1(YGR150C)|FD-Score:-4.46|P-value:4.05E-6||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CKB2(YOR039W)|FD-Score:4.49|P-value:3.53E-6||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNB1(YKL190W)|FD-Score:10.8|P-value:1.60E-27||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COA6(YMR244C-A_p)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:-10|P-value:5.44E-24||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-5.45|P-value:2.52E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX7(YMR256C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYS4(YGR155W)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DFG16(YOR030W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DGR2(YKL121W_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIG1(YPL049C)|FD-Score:4.84|P-value:6.43E-7||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DPP1(YDR284C)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DRS2(YAL026C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSS1(YMR287C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:EAR1(YMR171C)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:FMC1(YIL098C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GCN2(YDR283C)|FD-Score:10.9|P-value:5.41E-28||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:6.11|P-value:5.13E-10||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.63|P-value:9.15E-9||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCS1(YDL226C)|FD-Score:9.65|P-value:2.48E-22||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GYP1(YOR070C)|FD-Score:7.74|P-value:4.84E-15||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HIM1(YDR317W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:HMG1(YML075C)|FD-Score:5.56|P-value:1.32E-8||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOG1(YLR113W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HOM2(YDR158W)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HRD1(YOL013C)|FD-Score:4.54|P-value:2.87E-6||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:ICE2(YIL090W)|FD-Score:5.5|P-value:1.93E-8||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IMP2'(YIL154C)|FD-Score:11.3|P-value:5.15E-30||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISM1(YPL040C)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:IST2(YBR086C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process Gene:LAT1(YNL071W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LIP2(YLR239C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAL11(YGR289C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MEF2(YJL102W)|FD-Score:-4.02|P-value:2.91E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MET18(YIL128W)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MID2(YLR332W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRE11(YMR224C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPL32(YCR003W)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSH3(YCR092C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTG1(YMR097C)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NHP6B(YBR089C-A)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication Gene:PCS60(YBR222C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PFS1(YHR185C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHO84(YML123C)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:POA1(YBR022W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:POR1(YNL055C)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:REG1(YDR028C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPL2A(YFR031C-A)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:5.36|P-value:4.21E-8||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:5.22|P-value:8.71E-8||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC3(YHR087W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTC6(YPL183W-A)|FD-Score:-3.95|P-value:3.87E-5||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:4.98|P-value:3.14E-7||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC6(YDR129C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SGF73(YGL066W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIF2(YBR103W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SLG1(YOR008C)|FD-Score:-4.85|P-value:6.07E-7||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:7.85|P-value:2.12E-15||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT3(YDR392W)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:5.37|P-value:3.85E-8||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SSK1(YLR006C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:STE13(YOR219C)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:SYS1(YJL004C)|FD-Score:-4.45|P-value:4.33E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TRP1(YDR007W)|FD-Score:6.66|P-value:1.35E-11||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:10.1|P-value:1.79E-24||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.1|P-value:6.15E-13||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.71|P-value:1.51E-18||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TVP18(YMR071C)|FD-Score:9.3|P-value:6.83E-21||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBR2(YLR024C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:UPC2(YDR213W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:URA4(YLR420W)|FD-Score:6.86|P-value:3.55E-12||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:URE2(YNL229C)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VMA16(YHR026W)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS61(YDR136C_d)|FD-Score:-3.75|P-value:8.93E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:WSC2(YNL283C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBL010C(YBL010C_p)|FD-Score:9.38|P-value:3.23E-21||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YDL118W(YDL118W_p)|FD-Score:4.31|P-value:8.19E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:5.82|P-value:3.02E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR154C(YDR154C_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YFL034W(YFL034W_p)|FD-Score:11.3|P-value:4.15E-30||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YGL108C(YGL108C_p)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGL176C(YGL176C_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YJR079W(YJR079W_p)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YKL069W(YKL069W)|FD-Score:4.51|P-value:3.22E-6||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL136W(YKL136W_d)|FD-Score:4.44|P-value:4.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR169W(YLR169W_d)|FD-Score:5.26|P-value:7.14E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR171W(YLR171W_d)|FD-Score:6.8|P-value:5.13E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNG1(YOR064C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YOR008C-A(YOR008C-A_p)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres Gene:YOR034C-A(YOR034C-A_p)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL182C(YPL182C_d)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR027C(YPR027C_p)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Putative protein of unknown function Gene:YPT31(YER031C)|FD-Score:9.46|P-value:1.50E-21||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YSR3(YKR053C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL196W_d17.907.71E-726.45YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YAL038W11.401.39E-300.17CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YJL195C_d11.309.74E-301.83YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YLR208W9.441.92E-210.84SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YGL233W8.604.02E-180.59SEC15Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
YGL116W8.015.88E-160.53CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YER008C7.483.71E-140.66SEC3Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
YMR277W6.824.57E-120.72FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YHR007C6.105.37E-100.39ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YBR102C5.705.87E-90.06EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YMR218C5.658.20E-90.15TRS130One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
YIL068C5.501.94E-80.16SEC6Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
YPR055W5.344.68E-80.23SEC8Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
YDR413C_d5.111.59E-70.69YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YDR166C4.424.99E-60.11SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YLR170C11.602.83E-31APS1Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
YFL034W_p11.304.15E-30YFL034W_pPutative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
YIL154C11.305.15E-30IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YDR283C10.905.41E-28GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YKL190W10.801.60E-27CNB1Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress
YER090W10.101.79E-24TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDL226C9.652.48E-22GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YER031C9.461.50E-21YPT31Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
YBL010C_p9.383.23E-21YBL010C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
YMR071C9.306.83E-21TVP18Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
YDR354W8.711.51E-18TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YHR030C7.852.12E-15SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YOR070C7.744.84E-15GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YKL211C7.106.15E-13TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YLR420W6.863.55E-12URA4Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate

GO enrichment analysis for SGTC_1072
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5940SGTC_2778tioconazole 65.1 nMMiscellaneous54820.38806ergosterol depletion effects on membrane
0.5040SGTC_252miconazole 1.3 nMMiscellaneous41890.5ergosterol depletion effects on membrane
0.4383.52E-275SGTC_3204092-0846 5.3 μMChemDiv (Drug-like library)13593560.097561ergosterol depletion effects on membrane
0.4256.10E-258SGTC_6890833-0039 98.0 μMChemDiv (Drug-like library)53747260.101695ergosterol depletion effects on membrane
0.4083.34E-235SGTC_2014262-0159 12.8 μMChemDiv (Drug-like library)13410290.0547945ergosterol depletion effects on membrane
0.4077.13E-234SGTC_30189082602 49.5 μMChembridge (Drug-like library)173320890.15942
0.3932.17E-217SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.205479plasma membrane duress
0.3903.28E-214SGTC_3124092-0653 6.8 μMChemDiv (Drug-like library)11482780.0632911ergosterol depletion effects on membrane
0.3843.87E-206SGTC_10194262-0212 3.4 μMChemDiv (Drug-like library)46772130.0512821ergosterol depletion effects on membrane
0.3828.46E-204SGTC_57miconazole 162.5 nMChemDiv (Drug-like library)41890.5ergosterol depletion effects on membrane
0.3781.03E-199SGTC_2733amorolfine 100.0 μMMiscellaneous542600.05plasma membrane duress
0.3711.94E-192SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.0422535plasma membrane duress
0.3666.95E-187SGTC_262fenpropimorph 62.5 μMMiscellaneous916950.0519481ergosterol depletion effects on membrane
0.3644.36E-184SGTC_3084092-1169 104.0 μMChemDiv (Drug-like library)22685900.152778plasma membrane duress
0.3611.88E-181SGTC_29489070189 9.4 μMChembridge (Drug-like library)64736360.139241plasma membrane duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_252miconazole1.31 nM0.54189Miscellaneous416.128565.64502ergosterol depletion effects on membrane
SGTC_57miconazole162.5 nM0.54189ChemDiv (Drug-like library)416.128565.64502ergosterol depletion effects on membrane
SGTC_2778tioconazole65.09 nM0.388065482Miscellaneous387.711224.43703ergosterol depletion effects on membrane
SGTC_2735sertaconazole140 nM0.35616465863Miscellaneous437.76995.84203plasma membrane duress
SGTC_292890114849.79 μM0.2835822994751Chembridge (Drug-like library)366.0813.89812heme biosynthesis & mitochondrial translocase
SGTC_21065357045167.29 μM0.28125668842Chembridge (Fragment library)281.13733.01412
SGTC_1066ketoconazole628 nM0.277778456201NIH Clinical Collection531.430923.6106
SGTC_217ketoconazole989 nM0.277778456201Miscellaneous531.430923.6106ergosterol depletion effects on membrane
SGTC_2930901323014.89 μM0.2769232995857Chembridge (Drug-like library)315.23814.21612heme biosynthesis & mitochondrial translocase
SGTC_2777climbazole1.54 μM0.25373137907Miscellaneous292.760683.76603iron homeostasis
SGTC_7051124-0212310 μM0.259554485ChemDiv (Drug-like library)293.1484.01212
SGTC_2925901053632.5 μM0.252994177Chembridge (Drug-like library)314.809263.91112azole & statin