triclabendazole

6-chloro-5-(2,3-dichlorophenoxy)-2-methylsulfanyl-1H-benzimidazole

An antihelmetic and likely tubulin inhibitor.

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PubChem MeSH terms: Anthelmintics



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1073
Screen concentration 3.1 μM
Source NIH Clinical Collection
PubChem CID 50248
SMILES CSC1=NC2=CC(=C(C=C2N1)Cl)OC3=C(C(=CC=C3)Cl)Cl
Standardized SMILES CSc1nc2cc(Cl)c(Oc3cccc(Cl)c3Cl)cc2[nH]1
Molecular weight 359.6581
ALogP 5.98
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.91
% growth inhibition (Hom. pool) 5.34


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 50248
Download HIP data (tab-delimited text)  (excel)
Gene:DBP9(YLR276C)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.23||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:GPN2(YOR262W)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:KAR2(YJL034W)|FD-Score:3.12|P-value:9.15E-4|Clearance:0.02||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:MIA40(YKL195W)|FD-Score:3.12|P-value:8.91E-4|Clearance:0.01||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NUS1(YDL193W)|FD-Score:-3.91|P-value:4.64E-5|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:RET1(YOR207C)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.23||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPT6(YGL048C)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.01||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:TOM40(YMR203W)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL152W(YDL152W_d)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YGR114C(YGR114C_d)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:DBP9(YLR276C)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.23||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:GPN2(YOR262W)|FD-Score:-3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:KAR2(YJL034W)|FD-Score:3.12|P-value:9.15E-4|Clearance:0.02||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:MIA40(YKL195W)|FD-Score:3.12|P-value:8.91E-4|Clearance:0.01||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NUS1(YDL193W)|FD-Score:-3.91|P-value:4.64E-5|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:RET1(YOR207C)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.23||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPT6(YGL048C)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.01||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:TOM40(YMR203W)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.03||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL152W(YDL152W_d)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YGR114C(YGR114C_d)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 50248
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:6.66|P-value:1.32E-11||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADH3(YMR083W)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:ALD6(YPL061W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALG6(YOR002W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARC18(YLR370C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG82(YDR173C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARL3(YPL051W)|FD-Score:14.7|P-value:4.52E-49||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG1(YGL180W)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATP11(YNL315C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP12(YJL180C)|FD-Score:4.37|P-value:6.17E-6||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:BOR1(YNL275W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:CAP2(YIL034C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CLN3(YAL040C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CSN9(YDR179C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:CYB2(YML054C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DAL7(YIR031C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOA4(YDR069C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DSE2(YHR143W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DSF2(YBR007C_p)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EMI2(YDR516C)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:FAR11(YNL127W)|FD-Score:6.29|P-value:1.62E-10||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FMC1(YIL098C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GCR2(YNL199C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GEA2(YEL022W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLY1(YEL046C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GRR1(YJR090C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTR2(YGR163W)|FD-Score:5.63|P-value:9.11E-9||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HAL9(YOL089C)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HCR1(YLR192C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HNT2(YDR305C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HSL7(YBR133C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IWR1(YDL115C)|FD-Score:9.9|P-value:2.13E-23||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:JNM1(YMR294W)|FD-Score:5.3|P-value:5.72E-8||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LYP1(YNL268W)|FD-Score:-3.84|P-value:6.19E-5||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MET17(YLR303W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET18(YIL128W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MET6(YER091C)|FD-Score:5.93|P-value:1.49E-9||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:MFT1(YML062C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MNE1(YOR350C)|FD-Score:5.69|P-value:6.17E-9||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MPC54(YOR177C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPS5(YBR251W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC3(YLR219W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:MSP1(YGR028W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase Gene:NMD2(YHR077C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:PAF1(YBR279W)|FD-Score:5.01|P-value:2.71E-7||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PDR10(YOR328W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PEX10(YDR265W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PHO4(YFR034C)|FD-Score:6.41|P-value:7.11E-11||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:POL32(YJR043C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:POP2(YNR052C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PXL1(YKR090W)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD17(YOR368W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RCN1(YKL159C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REC102(YLR329W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:REI1(YBR267W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RHR2(YIL053W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL37B(YDR500C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RTS3(YGR161C_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SHC1(YER096W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLA1(YBL007C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLH1(YGR271W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SNL1(YIL016W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SSA3(YBL075C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSO1(YPL232W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:TAT1(YBR069C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI4(YGR144W)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:UBC8(YEL012W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UFO1(YML088W)|FD-Score:4.6|P-value:2.07E-6||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VAC7(YNL054W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VAN1(YML115C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VHC1(YBR235W_p)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:YAP3(YHL009C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL010C(YBL010C_p)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBR178W(YBR178W_d)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR050C(YCR050C_p)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YCR061W(YCR061W_p)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YCR085W(YCR085W_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL088W(YGL088W_d)|FD-Score:6.44|P-value:6.05E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YIL067C(YIL067C_p)|FD-Score:5.9|P-value:1.79E-9||SGD DESC:Uncharacterized protein of unknown function Gene:YJR107W(YJR107W_p)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Putative lipase Gene:YLR283W(YLR283W_p)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YLR285C-A(YLR285C-A_p)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR294C(YLR294C_d)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YML007C-A(YML007C-A_p)|FD-Score:4.92|P-value:4.23E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YPL136W(YPL136W_d)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR014C(YPR014C_d)|FD-Score:4.89|P-value:5.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YTA6(YPL074W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:ACE2(YLR131C)|FD-Score:6.66|P-value:1.32E-11||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADH3(YMR083W)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:ALD6(YPL061W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ALG6(YOR002W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARC18(YLR370C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG82(YDR173C)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARL3(YPL051W)|FD-Score:14.7|P-value:4.52E-49||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATG1(YGL180W)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATP11(YNL315C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP12(YJL180C)|FD-Score:4.37|P-value:6.17E-6||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:BOR1(YNL275W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:CAP2(YIL034C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CLN3(YAL040C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CSN9(YDR179C)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling Gene:CYB2(YML054C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DAL7(YIR031C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOA4(YDR069C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DSE2(YHR143W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DSF2(YBR007C_p)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EMI2(YDR516C)|FD-Score:4.62|P-value:1.90E-6||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:FAR11(YNL127W)|FD-Score:6.29|P-value:1.62E-10||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FMC1(YIL098C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GCR2(YNL199C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GEA2(YEL022W)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLY1(YEL046C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GRR1(YJR090C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTR2(YGR163W)|FD-Score:5.63|P-value:9.11E-9||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HAL9(YOL089C)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HCR1(YLR192C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HNT2(YDR305C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:HSL7(YBR133C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IWR1(YDL115C)|FD-Score:9.9|P-value:2.13E-23||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:JNM1(YMR294W)|FD-Score:5.3|P-value:5.72E-8||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LYP1(YNL268W)|FD-Score:-3.84|P-value:6.19E-5||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MET17(YLR303W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET18(YIL128W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MET6(YER091C)|FD-Score:5.93|P-value:1.49E-9||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:MFT1(YML062C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MNE1(YOR350C)|FD-Score:5.69|P-value:6.17E-9||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MPC54(YOR177C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPS5(YBR251W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC3(YLR219W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:MSP1(YGR028W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase Gene:NMD2(YHR077C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:PAF1(YBR279W)|FD-Score:5.01|P-value:2.71E-7||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PDR10(YOR328W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PEX10(YDR265W)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PHO4(YFR034C)|FD-Score:6.41|P-value:7.11E-11||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:POL32(YJR043C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:POP2(YNR052C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PXL1(YKR090W)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD17(YOR368W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RCN1(YKL159C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:REC102(YLR329W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:REI1(YBR267W)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RHR2(YIL053W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL37B(YDR500C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RTS3(YGR161C_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SHC1(YER096W)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SKY1(YMR216C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLA1(YBL007C)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLH1(YGR271W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SNL1(YIL016W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SSA3(YBL075C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSO1(YPL232W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:TAT1(YBR069C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI4(YGR144W)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:UBC8(YEL012W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UFO1(YML088W)|FD-Score:4.6|P-value:2.07E-6||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VAC7(YNL054W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VAN1(YML115C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VHC1(YBR235W_p)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:YAP3(YHL009C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL010C(YBL010C_p)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBR178W(YBR178W_d)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR050C(YCR050C_p)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YCR061W(YCR061W_p)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YCR085W(YCR085W_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR467C(YDR467C_d)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL088W(YGL088W_d)|FD-Score:6.44|P-value:6.05E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YIL067C(YIL067C_p)|FD-Score:5.9|P-value:1.79E-9||SGD DESC:Uncharacterized protein of unknown function Gene:YJR107W(YJR107W_p)|FD-Score:3.77|P-value:8.13E-5||SGD DESC:Putative lipase Gene:YLR283W(YLR283W_p)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YLR285C-A(YLR285C-A_p)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR294C(YLR294C_d)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YML007C-A(YML007C-A_p)|FD-Score:4.92|P-value:4.23E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YPL136W(YPL136W_d)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR014C(YPR014C_d)|FD-Score:4.89|P-value:5.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YTA6(YPL074W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR276C3.571.76E-40.23DBP9DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
YOR207C3.542.00E-40.23RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YGR114C_d3.452.82E-40.23YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YDL152W_d3.393.52E-40.23YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YMR203W3.167.95E-40.03TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YKL195W3.128.91E-40.01MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YJL034W3.129.15E-40.02KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YGL048C3.099.91E-40.01RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YOR319W3.080.001030.04HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YDL140C3.040.001180.02RPO21RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
YEL058W3.020.001250.06PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YDR407C2.960.001540.22TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YLR009W2.740.003100.06RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YGR280C2.680.003690.01PXR1Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
YNL290W2.660.003850.06RFC3Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL051W14.704.52E-49ARL3GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
YDL115C9.902.13E-23IWR1RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
YLR131C6.661.32E-11ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YGL088W_d6.446.05E-11YGL088W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing
YFR034C6.417.11E-11PHO4Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability
YNL127W6.291.62E-10FAR11Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2
YER091C5.931.49E-9MET6Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
YIL067C_p5.901.79E-9YIL067C_pUncharacterized protein of unknown function
YOR350C5.696.17E-9MNE1Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA
YGR163W5.639.11E-9GTR2Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
YMR294W5.305.72E-8JNM1Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
YBR279W5.012.71E-7PAF1Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1
YML007C-A_p4.924.23E-7YML007C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
YPR014C_d4.895.00E-7YPR014C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
YEL046C4.711.26E-6GLY1Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis

GO enrichment analysis for SGTC_1073
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1111.53E-17SGTC_31739105693 49.5 μMChembridge (Drug-like library)170966580.176471
0.1063.28E-16SGTC_14294049-0205 9.5 μMChemDiv (Drug-like library)227744780.0735294
0.0961.33E-13SGTC_7313966-0296 387.0 μMChemDiv (Drug-like library)6609560.0779221
0.0904.30E-12SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0757576Golgi
0.0881.27E-11SGTC_28909046729 58.4 μMChembridge (Drug-like library)9033290.166667Golgi
0.0872.39E-11SGTC_2630antimycin a 100.0 μMMicrosource (Natural product library)57021990.0693069mitochondrial processes
0.0862.97E-11SGTC_1087pterostilbene 9.1 μMNIH Clinical Collection52817270.101695
0.0856.75E-11SGTC_28849058502 52.0 μMChembridge (Drug-like library)64715540.0985916
0.0849.01E-11SGTC_1603453-0671 289.7 μMChemDiv (Drug-like library)53222680.0909091
0.0808.57E-10SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.0757576Golgi
0.0781.86E-9SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0746269Golgi
0.0764.46E-9SGTC_389tpck 16.7 μMMiscellaneous4396470.0821918
0.0741.40E-8SGTC_6541124-0271 80.9 μMChemDiv (Drug-like library)54037480.0657895
0.0741.53E-8SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.115385Golgi
0.0723.56E-8SGTC_9113456-4043 236.0 μMChemDiv (Drug-like library)9142810.0945946mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_15120259-054412.2 μM0.28333318476ChemDiv (Drug-like library)293.1484.43412
SGTC_3174910720449.47 μM0.260873938802Chembridge (Drug-like library)337.86753.91413ERG2
SGTC_1704197-0004226 μM0.246154218692ChemDiv (Drug-like library)317.793283.72923
SGTC_558k064-002721.3 μM0.2459025088526ChemDiv (Drug-like library)334.79895.55113DNA damage response
SGTC_5382922-083853.3 μM0.237288777039ChemDiv (Drug-like library)259.305223.43222Golgi
SGTC_8380109-020371.4 μM0.232143759422ChemDiv (Drug-like library)208.258483.75611
SGTC_3020908914049.47 μM0.23188417217859Chembridge (Drug-like library)366.241783.42604
SGTC_6051315-0399119 μM0.22535224020461ChemDiv (Drug-like library)381.254666.69203Golgi
SGTC_22446635614200 μM0.224138836499Chembridge (Fragment library)234.079262.01512
SGTC_6941315-043843.2 μM0.2236846793154ChemDiv (Drug-like library)378.80843.90215