carmofur

5-fluoro-N-hexyl-2,4-dioxopyrimidine-1-carboxamide

Carmofur is a derivative of 5-fluorouracil (5-FU). 5-FU is a pyrimidine analog that inhibits thymidylate synthase and is metabolized into cytotoxic ribonucleotides and deoxyribonucleotides that can be incorporated into DNA and RNA. In addition to being incorporated in DNA and RNA, the drug has been shown to inhibit the essential ribonuclease activity of the exosome complex.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Antineoplastic Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1074
Screen concentration 3.8 μM
Source NIH Clinical Collection
PubChem CID 2577
SMILES CCCCCCNC(=O)N1C=C(C(=O)NC1=O)F
Standardized SMILES CCCCCCNC(=O)N1C=C(F)C(=O)NC1=O
Molecular weight 257.2614
ALogP 1.13
H-bond donor count 2
H-bond acceptor count 4
Response signature exosome

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.25
% growth inhibition (Hom. pool) 5.34


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2577
Download HIP data (tab-delimited text)  (excel)
Gene:BRX1(YOL077C)|FD-Score:7.29|P-value:1.53E-13|Clearance:0.12||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CNS1(YBR155W)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.11||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:COG4(YPR105C)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSL4(YNL232W)|FD-Score:4.77|P-value:9.00E-7|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:DIS3(YOL021C)|FD-Score:16|P-value:4.32E-58|Clearance:2.88||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:EBP2(YKL172W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.03||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:ERB1(YMR049C)|FD-Score:4.14|P-value:1.73E-5|Clearance:0.32||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:GLE1(YDL207W)|FD-Score:3.53|P-value:2.12E-4|Clearance:0.01||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:LSM2(YBL026W)|FD-Score:6.42|P-value:6.94E-11|Clearance:0.38||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAK11(YKL021C)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.04||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:MAK16(YAL025C)|FD-Score:3.12|P-value:9.02E-4|Clearance:0.04||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:MAK21(YDR060W)|FD-Score:7.17|P-value:3.64E-13|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:MTR3(YGR158C)|FD-Score:13.1|P-value:9.21E-40|Clearance:0.53||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:MTR4(YJL050W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.03||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NOC2(YOR206W)|FD-Score:4.49|P-value:3.63E-6|Clearance:0.08||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.01||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP15(YNL110C)|FD-Score:5.34|P-value:4.62E-8|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP2(YNL061W)|FD-Score:5.97|P-value:1.17E-9|Clearance:0.07||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP4(YPL043W)|FD-Score:5.36|P-value:4.09E-8|Clearance:0.02||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NOP58(YOR310C)|FD-Score:7.1|P-value:6.04E-13|Clearance:0.69||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RLP24(YLR009W)|FD-Score:5.9|P-value:1.84E-9|Clearance:0.53||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RLP7(YNL002C)|FD-Score:8.53|P-value:7.52E-18|Clearance:0.45||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPF2(YKR081C)|FD-Score:6.04|P-value:7.76E-10|Clearance:0.06||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL17A(YKL180W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL32(YBL092W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.07||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RRP15(YPR143W)|FD-Score:10.3|P-value:3.19E-25|Clearance:1.02||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP42(YDL111C)|FD-Score:9.29|P-value:7.67E-21|Clearance:0.76||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:12.6|P-value:9.13E-37|Clearance:2.3||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:4.4|P-value:5.29E-6|Clearance:0.09||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRS1(YOR294W)|FD-Score:3.59|P-value:1.63E-4|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RVB2(YPL235W)|FD-Score:3.82|P-value:6.64E-5|Clearance:0.08||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SKI6(YGR195W)|FD-Score:18.7|P-value:1.09E-78|Clearance:2.88||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SPB1(YCL054W)|FD-Score:4.32|P-value:7.85E-6|Clearance:0.11||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPC105(YGL093W)|FD-Score:5.98|P-value:1.15E-9|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SUP45(YBR143C)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.2||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TCP1(YDR212W)|FD-Score:-3.14|P-value:8.35E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFG1(YGR186W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.08||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TIF35(YDR429C)|FD-Score:-3.11|P-value:9.51E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YDL196W(YDL196W_d)|FD-Score:4.77|P-value:9.11E-7|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YPL044C(YPL044C_d)|FD-Score:5.27|P-value:6.66E-8|Clearance:0.5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:YPR142C(YPR142C_d)|FD-Score:8.07|P-value:3.39E-16|Clearance:0.78||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:YTH1(YPR107C)|FD-Score:3.74|P-value:9.25E-5|Clearance:0.07||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:BRX1(YOL077C)|FD-Score:7.29|P-value:1.53E-13|Clearance:0.12||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CNS1(YBR155W)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.11||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:COG4(YPR105C)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSL4(YNL232W)|FD-Score:4.77|P-value:9.00E-7|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:DIS3(YOL021C)|FD-Score:16|P-value:4.32E-58|Clearance:2.88||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:EBP2(YKL172W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.03||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:ERB1(YMR049C)|FD-Score:4.14|P-value:1.73E-5|Clearance:0.32||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:GLE1(YDL207W)|FD-Score:3.53|P-value:2.12E-4|Clearance:0.01||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:LSM2(YBL026W)|FD-Score:6.42|P-value:6.94E-11|Clearance:0.38||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAK11(YKL021C)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.04||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:MAK16(YAL025C)|FD-Score:3.12|P-value:9.02E-4|Clearance:0.04||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:MAK21(YDR060W)|FD-Score:7.17|P-value:3.64E-13|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:MTR3(YGR158C)|FD-Score:13.1|P-value:9.21E-40|Clearance:0.53||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:MTR4(YJL050W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.03||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NOC2(YOR206W)|FD-Score:4.49|P-value:3.63E-6|Clearance:0.08||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.01||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP15(YNL110C)|FD-Score:5.34|P-value:4.62E-8|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP2(YNL061W)|FD-Score:5.97|P-value:1.17E-9|Clearance:0.07||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP4(YPL043W)|FD-Score:5.36|P-value:4.09E-8|Clearance:0.02||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NOP58(YOR310C)|FD-Score:7.1|P-value:6.04E-13|Clearance:0.69||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RLP24(YLR009W)|FD-Score:5.9|P-value:1.84E-9|Clearance:0.53||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RLP7(YNL002C)|FD-Score:8.53|P-value:7.52E-18|Clearance:0.45||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPF2(YKR081C)|FD-Score:6.04|P-value:7.76E-10|Clearance:0.06||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL17A(YKL180W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL32(YBL092W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.07||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RRP15(YPR143W)|FD-Score:10.3|P-value:3.19E-25|Clearance:1.02||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP42(YDL111C)|FD-Score:9.29|P-value:7.67E-21|Clearance:0.76||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:12.6|P-value:9.13E-37|Clearance:2.3||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:4.4|P-value:5.29E-6|Clearance:0.09||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRS1(YOR294W)|FD-Score:3.59|P-value:1.63E-4|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RVB2(YPL235W)|FD-Score:3.82|P-value:6.64E-5|Clearance:0.08||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SKI6(YGR195W)|FD-Score:18.7|P-value:1.09E-78|Clearance:2.88||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SPB1(YCL054W)|FD-Score:4.32|P-value:7.85E-6|Clearance:0.11||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPC105(YGL093W)|FD-Score:5.98|P-value:1.15E-9|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SUP45(YBR143C)|FD-Score:3.32|P-value:4.44E-4|Clearance:0.2||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TCP1(YDR212W)|FD-Score:-3.14|P-value:8.35E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFG1(YGR186W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.08||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TIF35(YDR429C)|FD-Score:-3.11|P-value:9.51E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YDL196W(YDL196W_d)|FD-Score:4.77|P-value:9.11E-7|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YPL044C(YPL044C_d)|FD-Score:5.27|P-value:6.66E-8|Clearance:0.5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:YPR142C(YPR142C_d)|FD-Score:8.07|P-value:3.39E-16|Clearance:0.78||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:YTH1(YPR107C)|FD-Score:3.74|P-value:9.25E-5|Clearance:0.07||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2577
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ATG27(YJL178C)|FD-Score:-3.89|P-value:5.00E-5||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:ATS1(YAL020C)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:AZR1(YGR224W)|FD-Score:4.3|P-value:8.73E-6||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BMH1(YER177W)|FD-Score:3.8|P-value:7.09E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BUD3(YCL014W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CEM1(YER061C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CNB1(YKL190W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DFG5(YMR238W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DHH1(YDL160C)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DLD2(YDL178W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DMA1(YHR115C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DOC1(YGL240W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DYN3(YMR299C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM8(YBR076W)|FD-Score:5.01|P-value:2.67E-7||SGD DESC:Non-essential protein of unknown function Gene:EDS1(YBR033W_p)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:FMP27(YLR454W_p)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP37(YGL080W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FTR1(YER145C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FYV12(YOR183W_p)|FD-Score:4.28|P-value:9.45E-6||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAT3(YLR013W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GPR1(YDL035C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HVG1(YER039C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IDH2(YOR136W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IRC6(YFR043C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC10(YER180C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KAP123(YER110C)|FD-Score:6.55|P-value:2.87E-11||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LHP1(YDL051W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen Gene:MAK3(YPR051W)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MLH2(YLR035C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MNN2(YBR015C)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPL32(YCR003W)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSK1(YNL073W)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:OCA6(YDR067C_p)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PAP2(YOL115W)|FD-Score:4.36|P-value:6.58E-6||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PER1(YCR044C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET309(YLR067C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PHO81(YGR233C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PIF1(YML061C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RPL22B(YFL034C-A)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:RXT3(YDL076C)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:5.77|P-value:3.85E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SSO1(YPL232W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STB5(YHR178W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SVS1(YPL163C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:THI2(YBR240C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TIS11(YLR136C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:UGA2(YBR006W)|FD-Score:8.21|P-value:1.15E-16||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URA4(YLR420W)|FD-Score:4.79|P-value:8.33E-7||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VPS55(YJR044C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS65(YLR322W_d)|FD-Score:6.17|P-value:3.45E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:VPS71(YML041C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS75(YNL246W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YBR284W(YBR284W_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR149C(YDR149C_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YKL075C(YKL075C_p)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKR033C(YKR033C_d)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLR252W(YLR252W_d)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR285C-A(YLR285C-A_p)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Putative protein of unknown function Gene:YMR027W(YMR027W_p)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR265C(YMR265C_p)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Putative protein of unknown function Gene:YOR263C(YOR263C_d)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YVH1(YIR026C)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ACB1(YGR037C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ATG27(YJL178C)|FD-Score:-3.89|P-value:5.00E-5||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:ATS1(YAL020C)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:AZR1(YGR224W)|FD-Score:4.3|P-value:8.73E-6||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BMH1(YER177W)|FD-Score:3.8|P-value:7.09E-5||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BUD3(YCL014W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CEM1(YER061C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CNB1(YKL190W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DFG5(YMR238W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DHH1(YDL160C)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DLD2(YDL178W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DMA1(YHR115C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DOC1(YGL240W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DYN3(YMR299C)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM8(YBR076W)|FD-Score:5.01|P-value:2.67E-7||SGD DESC:Non-essential protein of unknown function Gene:EDS1(YBR033W_p)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:FMP27(YLR454W_p)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP37(YGL080W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FTR1(YER145C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:FYV12(YOR183W_p)|FD-Score:4.28|P-value:9.45E-6||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAT3(YLR013W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GPR1(YDL035C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HVG1(YER039C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IDH2(YOR136W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IRC6(YFR043C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISC10(YER180C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KAP123(YER110C)|FD-Score:6.55|P-value:2.87E-11||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:LHP1(YDL051W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:RNA binding protein required for maturation of tRNA and U6 snRNA precursors; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen Gene:MAK3(YPR051W)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MLH2(YLR035C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MNN2(YBR015C)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRPL32(YCR003W)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSK1(YNL073W)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:OCA6(YDR067C_p)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:PAP2(YOL115W)|FD-Score:4.36|P-value:6.58E-6||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PER1(YCR044C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET309(YLR067C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PHO81(YGR233C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PIF1(YML061C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:RPL22B(YFL034C-A)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RTT106(YNL206C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:RXT3(YDL076C)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:5.77|P-value:3.85E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SSO1(YPL232W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STB5(YHR178W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SVS1(YPL163C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:THI2(YBR240C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TIS11(YLR136C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:UGA2(YBR006W)|FD-Score:8.21|P-value:1.15E-16||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URA4(YLR420W)|FD-Score:4.79|P-value:8.33E-7||SGD DESC:Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate Gene:VPS55(YJR044C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS65(YLR322W_d)|FD-Score:6.17|P-value:3.45E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:VPS71(YML041C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS75(YNL246W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YBR284W(YBR284W_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDR149C(YDR149C_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YKL075C(YKL075C_p)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKR033C(YKR033C_d)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLR252W(YLR252W_d)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR285C-A(YLR285C-A_p)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Putative protein of unknown function Gene:YMR027W(YMR027W_p)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR265C(YMR265C_p)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Putative protein of unknown function Gene:YOR263C(YOR263C_d)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YVH1(YIR026C)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR195W18.701.09E-782.88SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YOL021C16.004.32E-582.88DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YGR158C13.109.21E-400.53MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YDR280W12.609.13E-372.30RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YPR143W10.303.19E-251.02RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YDL111C9.297.67E-210.76RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YNL002C8.537.52E-180.45RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YPR142C_d8.073.39E-160.78YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YOL077C7.291.53E-130.12BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
YDR060W7.173.64E-130.07MAK21Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
YOR310C7.106.04E-130.69NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YBL026W6.426.94E-110.38LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YKR081C6.047.76E-100.06RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YGL093W5.981.15E-90.00SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YNL061W5.971.17E-90.07NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR006W8.211.15E-16UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YER110C6.552.87E-11KAP123Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
YLR322W_d6.173.45E-10VPS65_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth
YKL212W5.773.85E-9SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YAL020C5.581.22E-8ATS1Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle
YNL073W5.511.81E-8MSK1Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys)
YBR076W5.012.67E-7ECM8Non-essential protein of unknown function
YLR420W4.798.33E-7URA4Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
YFL034C-A4.671.49E-6RPL22BRibosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
YBR015C4.651.65E-6MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YDL160C4.602.10E-6DHH1Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress
YOL115W4.366.58E-6PAP2Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p
YGR224W4.308.73E-6AZR1Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole
YOR183W_p4.289.45E-6FYV12_pProtein of unknown function, required for survival upon exposure to K1 killer toxin
YKL075C_p4.221.21E-5YKL075C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin

GO enrichment analysis for SGTC_1074
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5320SGTC_10775-fluorocytosine 377.0 nMNIH Clinical Collection33660.153846exosome
0.5190SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33660.153846exosome
0.5180SGTC_6104215-0184 155.0 μMChemDiv (Drug-like library)56050150.0853659exosome
0.4121.72E-240SGTC_4235-fluorouracil 457.0 μMMiscellaneous33850.23913exosome
0.3971.52E-220SGTC_2425hr heat shock (37°C) + 5-fluorouracil 4.6 μMMiscellaneous33850.23913heat shock/prefoldin
0.3462.54E-165SGTC_2615-fluorouridine 15.4 μMMiscellaneous18210.293103exosome
0.2806.37E-107SGTC_6173770-0098 35.0 μMChemDiv (Drug-like library)59626390.105263exosome
0.1761.69E-42SGTC_33379144342 33.5 μMChembridge (Drug-like library)176829490.123288
0.1625.29E-36SGTC_1180083-0097 135.3 μMChemDiv (Drug-like library)52073560.0681818translation
0.1288.36E-23SGTC_21925915882 198.5 μMChembridge (Fragment library)28761490.111111RNA processing & uracil transport
0.1031.92E-15SGTC_2151287-0124 461.5 μMChemDiv (Drug-like library)55608630.0352941
0.0961.34E-13SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.0813954translation
0.0952.37E-13SGTC_1745st081495 74.0 μMTimTec (Natural product derivative library)138424020.0694444RNA processing & uracil transport
0.0953.28E-13SGTC_8790831-1030 162.0 μMChemDiv (Drug-like library)15492520.0677966
0.0897.01E-12SGTC_12941000-1494 117.0 μMChemDiv (Drug-like library)7127970.0857143

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3252913587149.47 μM0.32758617682838Chembridge (Drug-like library)245.27711.51122
SGTC_2615-fluorouridine15.38 μM0.2931031821Miscellaneous262.191843-2.54147exosome
SGTC_7313966-0296387 μM0.265625660956ChemDiv (Drug-like library)318.367582.44314
SGTC_13341486-0610236 μM0.2615383742339ChemDiv (Drug-like library)338.468244.2011460S ribosome export
SGTC_6731082-0474110 μM0.2586213098046ChemDiv (Drug-like library)247.246621.8414RSC complex & mRNA processing
SGTC_1899st06325459.6 μM0.2580653093813TimTec (Natural product derivative library)335.444463.96714
SGTC_12760871-006894.2 μM0.253414906ChemDiv (Drug-like library)261.319562.57104
SGTC_477mead ethanolamide10 μM0.24137916061185ICCB bioactive library349.550565.92222
SGTC_810411-00469.97 μM0.24074112925265ChemDiv (Drug-like library)297.230645.4701
SGTC_2425hr heat shock (37°C) + 5-fluorouracil4.57 μM0.239133385Miscellaneous130.077223-1.10223heat shock/prefoldin