5-fluorocytosine

6-amino-5-fluoro-1H-pyrimidin-2-one

The mechanism of 5-fluorocytosine (5-FC) is identical to 5-fluorouracil (5-FU) and acts by inhibiting thymidylate synthetase and through metabolism into cytotoxic ribonucleotides and deoxyribonucleotides that can be incorporated into DNA and RNA. In addition, the drug has been shown to inhibit the essential ribonuclease activity of the exosome complex. The specificity of 5-FC for fungi is due to the requirement for the fungal specific cytosine permease in addition to cytosine deaminase that acts to convert 5-FC to 5-FU.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1077
Screen concentration 377.0 nM
Source NIH Clinical Collection
PubChem CID 3366
SMILES C1=NC(=O)NC(=C1F)N
Standardized SMILES NC1=C(F)C=NC(=O)N1
Molecular weight 129.0925
ALogP -0.62
H-bond donor count 2
H-bond acceptor count 3
Response signature exosome

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.79
% growth inhibition (Hom. pool) 2.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3366
Download HIP data (tab-delimited text)  (excel)
Gene:BRX1(YOL077C)|FD-Score:4.39|P-value:5.66E-6|Clearance:0.18||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CFT1(YDR301W)|FD-Score:3.16|P-value:7.99E-4|Clearance:0.08||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:CSL4(YNL232W)|FD-Score:8.86|P-value:4.17E-19|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:DBF4(YDR052C)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.05||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DIS3(YOL021C)|FD-Score:12.3|P-value:7.64E-35|Clearance:1.07||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:EBP2(YKL172W)|FD-Score:5.55|P-value:1.47E-8|Clearance:0.02||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:ERB1(YMR049C)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:LSM2(YBL026W)|FD-Score:3.73|P-value:9.60E-5|Clearance:0.28||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAK11(YKL021C)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.17||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:MAK21(YDR060W)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.15||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:MTR3(YGR158C)|FD-Score:7.95|P-value:9.22E-16|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NAM9(YNL137C)|FD-Score:3.3|P-value:4.77E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NOP15(YNL110C)|FD-Score:5.7|P-value:5.91E-9|Clearance:0.16||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP4(YPL043W)|FD-Score:5|P-value:2.92E-7|Clearance:0.61||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NOP58(YOR310C)|FD-Score:5.52|P-value:1.66E-8|Clearance:0.53||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RLP24(YLR009W)|FD-Score:3.16|P-value:7.97E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RLP7(YNL002C)|FD-Score:6.88|P-value:2.93E-12|Clearance:0.22||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RRP15(YPR143W)|FD-Score:11.4|P-value:3.09E-30|Clearance:1.07||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP42(YDL111C)|FD-Score:13.1|P-value:1.53E-39|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:11.8|P-value:2.29E-32|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:6.66|P-value:1.39E-11|Clearance:0.96||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP9(YPR137W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.1||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SKI6(YGR195W)|FD-Score:10.8|P-value:1.13E-27|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:TIF35(YDR429C)|FD-Score:-3.81|P-value:6.84E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YPL044C(YPL044C_d)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:YPR142C(YPR142C_d)|FD-Score:9.81|P-value:5.30E-23|Clearance:1.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing Gene:BRX1(YOL077C)|FD-Score:4.39|P-value:5.66E-6|Clearance:0.18||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CFT1(YDR301W)|FD-Score:3.16|P-value:7.99E-4|Clearance:0.08||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:CSL4(YNL232W)|FD-Score:8.86|P-value:4.17E-19|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:DBF4(YDR052C)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.05||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DIS3(YOL021C)|FD-Score:12.3|P-value:7.64E-35|Clearance:1.07||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:EBP2(YKL172W)|FD-Score:5.55|P-value:1.47E-8|Clearance:0.02||SGD DESC:Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering Gene:ERB1(YMR049C)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:LSM2(YBL026W)|FD-Score:3.73|P-value:9.60E-5|Clearance:0.28||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAK11(YKL021C)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.17||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:MAK21(YDR060W)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.15||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:MTR3(YGR158C)|FD-Score:7.95|P-value:9.22E-16|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NAM9(YNL137C)|FD-Score:3.3|P-value:4.77E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NOP15(YNL110C)|FD-Score:5.7|P-value:5.91E-9|Clearance:0.16||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP4(YPL043W)|FD-Score:5|P-value:2.92E-7|Clearance:0.61||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:NOP58(YOR310C)|FD-Score:5.52|P-value:1.66E-8|Clearance:0.53||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RLP24(YLR009W)|FD-Score:3.16|P-value:7.97E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RLP7(YNL002C)|FD-Score:6.88|P-value:2.93E-12|Clearance:0.22||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RRP15(YPR143W)|FD-Score:11.4|P-value:3.09E-30|Clearance:1.07||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP42(YDL111C)|FD-Score:13.1|P-value:1.53E-39|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:RRP45(YDR280W)|FD-Score:11.8|P-value:2.29E-32|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RRP46(YGR095C)|FD-Score:6.66|P-value:1.39E-11|Clearance:0.96||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:RRP9(YPR137W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0.1||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SKI6(YGR195W)|FD-Score:10.8|P-value:1.13E-27|Clearance:1.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:TIF35(YDR429C)|FD-Score:-3.81|P-value:6.84E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YPL044C(YPL044C_d)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:YPR142C(YPR142C_d)|FD-Score:9.81|P-value:5.30E-23|Clearance:1.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3366
Download HOP data (tab-delimited text)  (excel)
Gene:AAD4(YDL243C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADE16(YLR028C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AIR2(YDL175C)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALG5(YPL227C)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARL3(YPL051W)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATO3(YDR384C)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BEM3(YPL115C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BUD3(YCL014W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CAF16(YFL028C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CAT2(YML042W)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:COG6(YNL041C)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRG1(YHR209W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DBP7(YKR024C)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:END3(YNL084C)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERF2(YLR246W)|FD-Score:-3.79|P-value:7.44E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:ETR1(YBR026C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FLC1(YPL221W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:GEA2(YEL022W)|FD-Score:-3.87|P-value:5.46E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:-3.72|P-value:9.91E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:IRC23(YOR044W)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:KRE6(YPR159W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LDB7(YBL006C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LST4(YKL176C)|FD-Score:5.68|P-value:6.61E-9||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LTE1(YAL024C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MCR1(YKL150W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MLS1(YNL117W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MNN10(YDR245W)|FD-Score:4.28|P-value:9.33E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MRPL23(YOR150W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:PDR11(YIL013C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PGM3(YMR278W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PMP1(YCR024C-A)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SCP1(YOR367W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLG1(YOR008C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNT309(YPR101W)|FD-Score:5.48|P-value:2.10E-8||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SSA3(YBL075C)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA11(YHR159W_p)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TVP18(YMR071C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMA11(YPL234C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:YCR006C(YCR006C_d)|FD-Score:7.79|P-value:3.31E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR043C(YCR043C_p)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDL144C(YDL144C_p)|FD-Score:4.93|P-value:4.18E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YER076C(YER076C_p)|FD-Score:5.42|P-value:2.98E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER188W(YER188W_d)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGL149W(YGL149W_d)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR146C-A(YGR146C-A_p)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YIR035C(YIR035C_p)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YKR045C(YKR045C_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YNL022C(YNL022C_p)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YOL107W(YOL107W_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR200W(YOR200W_d)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPR027C(YPR027C_p)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Putative protein of unknown function Gene:AAD4(YDL243C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADE16(YLR028C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AIR2(YDL175C)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALG5(YPL227C)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARL3(YPL051W)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATO3(YDR384C)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BEM3(YPL115C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BUD3(YCL014W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CAF16(YFL028C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CAT2(YML042W)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:COG6(YNL041C)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRG1(YHR209W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DBP7(YKR024C)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:END3(YNL084C)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERF2(YLR246W)|FD-Score:-3.79|P-value:7.44E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:ETR1(YBR026C)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FLC1(YPL221W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:GEA2(YEL022W)|FD-Score:-3.87|P-value:5.46E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:-3.72|P-value:9.91E-5||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:IRC23(YOR044W)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:KRE6(YPR159W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LDB7(YBL006C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LST4(YKL176C)|FD-Score:5.68|P-value:6.61E-9||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LTE1(YAL024C)|FD-Score:3.17|P-value:7.67E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MCR1(YKL150W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MLS1(YNL117W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MNN10(YDR245W)|FD-Score:4.28|P-value:9.33E-6||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MRPL23(YOR150W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:PDR11(YIL013C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PGM3(YMR278W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PMP1(YCR024C-A)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SCP1(YOR367W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLG1(YOR008C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNT309(YPR101W)|FD-Score:5.48|P-value:2.10E-8||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SSA3(YBL075C)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA11(YHR159W_p)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TVP18(YMR071C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VMA11(YPL234C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:YCR006C(YCR006C_d)|FD-Score:7.79|P-value:3.31E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR043C(YCR043C_p)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDL144C(YDL144C_p)|FD-Score:4.93|P-value:4.18E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YER076C(YER076C_p)|FD-Score:5.42|P-value:2.98E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER188W(YER188W_d)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGL149W(YGL149W_d)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGR146C-A(YGR146C-A_p)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YIR035C(YIR035C_p)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Putative cytoplasmic short-chain dehydrogenase/reductase Gene:YKR045C(YKR045C_p)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YNL022C(YNL022C_p)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Putative cytosine 5-methyltransferase, contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis Gene:YOL107W(YOL107W_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR200W(YOR200W_d)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPR027C(YPR027C_p)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL111C13.101.53E-391.07RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YOL021C12.307.64E-351.07DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YDR280W11.802.29E-321.07RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YPR143W11.403.09E-301.07RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YGR195W10.801.13E-271.07SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YPR142C_d9.815.30E-231.07YPR142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
YNL232W8.864.17E-191.07CSL4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
YGR158C7.959.22E-161.07MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YNL002C6.882.93E-120.23RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YGR095C6.661.39E-110.96RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YNL110C5.705.91E-90.16NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YKL172W5.551.47E-80.02EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
YOR310C5.521.66E-80.53NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YPL043W5.002.92E-70.61NOP4Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
YOL077C4.395.66E-60.18BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR006C_d7.793.31E-15YCR006C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKL176C5.686.61E-9LST4Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
YPR101W5.482.10E-8SNT309Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
YER076C_p5.422.98E-8YER076C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
YDL144C_p4.934.18E-7YDL144C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress
YNL041C4.751.01E-6COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YER188W_d4.701.31E-6YER188W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p
YOR367W4.691.34E-6SCP1Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin
YHR209W4.651.69E-6CRG1S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
YOR200W_d4.405.37E-6YOR200W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c
YDR245W4.289.33E-6MNN10Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
YIR035C_p4.141.70E-5YIR035C_pPutative cytoplasmic short-chain dehydrogenase/reductase
YMR278W4.141.70E-5PGM3Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
YPR027C_p4.121.89E-5YPR027C_pPutative protein of unknown function
YBL075C4.052.51E-5SSA3ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1077
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5320SGTC_1074carmofur 3.8 μMNIH Clinical Collection25770.153846exosome
0.5280SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33661exosome
0.4850SGTC_6104215-0184 155.0 μMChemDiv (Drug-like library)56050150.0895522exosome
0.4213.72E-252SGTC_4235-fluorouracil 457.0 μMMiscellaneous33850.366667exosome
0.3654.71E-185SGTC_2425hr heat shock (37°C) + 5-fluorouracil 4.6 μMMiscellaneous33850.366667heat shock/prefoldin
0.3551.88E-175SGTC_2615-fluorouridine 15.4 μMMiscellaneous18210.156863exosome
0.2802.04E-106SGTC_6173770-0098 35.0 μMChemDiv (Drug-like library)59626390.0625exosome
0.1852.03E-46SGTC_33379144342 33.5 μMChembridge (Drug-like library)176829490.1
0.1454.32E-29SGTC_1180083-0097 135.3 μMChemDiv (Drug-like library)52073560.0540541translation
0.1231.86E-21SGTC_2151287-0124 461.5 μMChemDiv (Drug-like library)55608630.0434783
0.1193.83E-20SGTC_8790831-1030 162.0 μMChemDiv (Drug-like library)15492520.0930233
0.1032.34E-15SGTC_3051682-2595 20.8 μMChemDiv (Drug-like library)2260330.0588235RNA processing & uracil transport
0.0952.01E-13SGTC_32219128209 49.5 μMChembridge (Drug-like library)164607230.0645161
0.0952.27E-13SGTC_31789107506 49.5 μMChembridge (Drug-like library)88971940.047619
0.0912.23E-12SGTC_750159-0059 199.0 μMChemDiv (Drug-like library)53772700.0535714fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_16985-fluorocytosine1.06 μM13366TimTec (Natural product derivative library)129.092463-0.61823exosome
SGTC_2425hr heat shock (37°C) + 5-fluorouracil4.57 μM0.3666673385Miscellaneous130.077223-1.10223heat shock/prefoldin
SGTC_4235-fluorouracil457 μM0.3666673385Miscellaneous130.077223-1.10223exosome
SGTC_22927671323168.05 μM0.229167937504Chembridge (Fragment library)251.2800032.02324RNA processing & uracil transport
SGTC_22297354935190 μM0.204545318112Chembridge (Fragment library)237.256641.74722RSC complex & mRNA processing
SGTC_20114012611177 μM0.1752049887Chembridge (Fragment library)168.1914031.91414mitochondrial processes
SGTC_23939031399185.94 μM0.1739131526623Chembridge (Fragment library)218.212-0.59324RNA processing & uracil transport
SGTC_20474141938200 μM0.1707323807862Chembridge (Fragment library)169.1530231.14414
SGTC_2237666774747.22 μM0.170213729940Chembridge (Fragment library)236.2653632.24314
SGTC_1879gemcitabine100 μM0.16666760750Miscellaneous263.198146-1.39436