idebenone FDA approved compound

2-(10-hydroxydecyl)-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione

A short-chain Coenzyme Q analog that enhances superoxide formation, presumably by mediating electron transfer from N2 to oxygen.

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PubChem MeSH terms: Antioxidants



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1078
Screen concentration 36.1 μM
Source Miscellaneous
PubChem CID 3686
SMILES CC1=C(C(=O)C(=C(C1=O)OC)OC)CCCCCCCCCCO
Standardized SMILES COC1=C(OC)C(=O)C(=C(C)C1=O)CCCCCCCCCCO
Molecular weight 338.4385
ALogP 3.84
H-bond donor count 1
H-bond acceptor count 5
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 19.1
% growth inhibition (Hom. pool) 7.96


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3686
Download HIP data (tab-delimited text)  (excel)
Gene:CCT8(YJL008C)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ERG20(YJL167W)|FD-Score:-5.25|P-value:7.64E-8|Clearance:0||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:GLN4(YOR168W)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.16||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:ILV5(YLR355C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.06||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IPP1(YBR011C)|FD-Score:4.03|P-value:2.81E-5|Clearance:0.03||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:KAE1(YKR038C)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.32||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:NIP1(YMR309C)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.01||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP15(YNL110C)|FD-Score:3.29|P-value:5.04E-4|Clearance:0.16||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP58(YOR310C)|FD-Score:5.48|P-value:2.16E-8|Clearance:1.38||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RPL5(YPL131W)|FD-Score:3.99|P-value:3.26E-5|Clearance:0.16||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEC14(YMR079W)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SNU56(YDR240C)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.07||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:YBR190W(YBR190W_d)|FD-Score:5.6|P-value:1.05E-8|Clearance:1.38||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:-3.17|P-value:7.52E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:CCT8(YJL008C)|FD-Score:3.13|P-value:8.86E-4|Clearance:0.11||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ERG20(YJL167W)|FD-Score:-5.25|P-value:7.64E-8|Clearance:0||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:GLN4(YOR168W)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.16||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:ILV5(YLR355C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.06||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IPP1(YBR011C)|FD-Score:4.03|P-value:2.81E-5|Clearance:0.03||SGD DESC:Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase Gene:KAE1(YKR038C)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.32||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:NIP1(YMR309C)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.01||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP15(YNL110C)|FD-Score:3.29|P-value:5.04E-4|Clearance:0.16||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP58(YOR310C)|FD-Score:5.48|P-value:2.16E-8|Clearance:1.38||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RPL5(YPL131W)|FD-Score:3.99|P-value:3.26E-5|Clearance:0.16||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:SEC14(YMR079W)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SNU56(YDR240C)|FD-Score:4.1|P-value:2.08E-5|Clearance:0.07||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:YBR190W(YBR190W_d)|FD-Score:5.6|P-value:1.05E-8|Clearance:1.38||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YDL196W(YDL196W_d)|FD-Score:-3.17|P-value:7.52E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3686
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:4.97|P-value:3.26E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGC1(YPR021C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ALG6(YOR002W)|FD-Score:5.78|P-value:3.63E-9||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APE3(YBR286W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APS1(YLR170C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:AVT6(YER119C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication Gene:BEM3(YPL115C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:CHL4(YDR254W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:COG6(YNL041C)|FD-Score:6.18|P-value:3.19E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:13.4|P-value:5.78E-41||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:13|P-value:5.88E-39||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX14(YML129C)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CPR3(YML078W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:CSR2(YPR030W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CTR1(YPR124W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CWP2(YKL096W-A)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DAL82(YNL314W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DOG1(YHR044C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DSK2(YMR276W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:ERG4(YGL012W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERJ5(YFR041C)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FUI1(YBL042C)|FD-Score:4.9|P-value:4.83E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GIS3(YLR094C)|FD-Score:7.92|P-value:1.22E-15||SGD DESC:Protein of unknown function Gene:GLY1(YEL046C)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GMH1(YKR030W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GOS1(YHL031C)|FD-Score:4.28|P-value:9.30E-6||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPB2(YAL056W)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GPX2(YBR244W)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress Gene:GSY2(YLR258W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GUP1(YGL084C)|FD-Score:4.43|P-value:4.79E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:IRS4(YKR019C)|FD-Score:8.03|P-value:4.83E-16||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KNS1(YLL019C)|FD-Score:-3.77|P-value:8.10E-5||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LST7(YGR057C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAC1(YMR021C)|FD-Score:6.27|P-value:1.77E-10||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET18(YIL128W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MOT3(YMR070W)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRS2(YOR334W)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MSA1(YOR066W)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:NBP2(YDR162C)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NUM1(YDR150W)|FD-Score:5.81|P-value:3.12E-9||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:ORM1(YGR038W)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OST6(YML019W)|FD-Score:4.31|P-value:8.35E-6||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PAD1(YDR538W)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PER33(YLR064W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PKR1(YMR123W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:4.85|P-value:6.31E-7||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PSY2(YNL201C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PUS4(YNL292W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:REV7(YIL139C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGP1(YDR137W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM21(YNL294C)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPE1(YJL121C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL24A(YGL031C)|FD-Score:5|P-value:2.91E-7||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS21B(YJL136C)|FD-Score:5.05|P-value:2.21E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:8.19|P-value:1.27E-16||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RTT107(YHR154W)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SER3(YER081W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SFT2(YBL102W)|FD-Score:5.43|P-value:2.80E-8||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SIN3(YOL004W)|FD-Score:4.91|P-value:4.62E-7||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SIT4(YDL047W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKN7(YHR206W)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLG1(YOR008C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNT309(YPR101W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOD1(YJR104C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SQS1(YNL224C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SRB5(YGR104C)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SUR4(YLR372W)|FD-Score:8.55|P-value:6.14E-18||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYS1(YJL004C)|FD-Score:9.05|P-value:7.15E-20||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TLG2(YOL018C)|FD-Score:6.62|P-value:1.79E-11||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TSA1(YML028W)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:10.4|P-value:1.00E-25||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBR2(YLR024C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:UPS2(YLR168C)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:URC2(YDR520C_p)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VPS1(YKR001C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XRS2(YDR369C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YAP1(YML007W)|FD-Score:8.69|P-value:1.88E-18||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YAT2(YER024W)|FD-Score:-4.49|P-value:3.57E-6||SGD DESC:Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane Gene:YDR003W-A(YDR003W-A_p)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR095C(YDR095C_d)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR476C(YDR476C_p)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YDR535C(YDR535C_d)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEL1(YBL060W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER077C(YER077C_p)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGR079W(YGR079W_p)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YIA6(YIL006W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL002W-A(YIL002W-A_p)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL147C(YJL147C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKL202W(YKL202W_d)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR283W(YLR283W_p)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YNL203C(YNL203C_d)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL099C(YOL099C_d)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR077C(YPR077C_d)|FD-Score:5.76|P-value:4.31E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR123C(YPR123C_d)|FD-Score:4.37|P-value:6.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR148C(YPR148C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:AFT1(YGL071W)|FD-Score:4.97|P-value:3.26E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGC1(YPR021C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ALG6(YOR002W)|FD-Score:5.78|P-value:3.63E-9||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APE3(YBR286W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APS1(YLR170C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:AVT6(YER119C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication Gene:BEM3(YPL115C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:CHL4(YDR254W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:COG6(YNL041C)|FD-Score:6.18|P-value:3.19E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:13.4|P-value:5.78E-41||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:13|P-value:5.88E-39||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX14(YML129C)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:CPR3(YML078W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:CSR2(YPR030W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CTR1(YPR124W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CWP2(YKL096W-A)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DAL82(YNL314W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DOG1(YHR044C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DSK2(YMR276W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:ERG4(YGL012W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERJ5(YFR041C)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FUI1(YBL042C)|FD-Score:4.9|P-value:4.83E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GIS3(YLR094C)|FD-Score:7.92|P-value:1.22E-15||SGD DESC:Protein of unknown function Gene:GLY1(YEL046C)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GMH1(YKR030W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Golgi membrane protein of unknown function, interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting Gene:GOS1(YHL031C)|FD-Score:4.28|P-value:9.30E-6||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPB2(YAL056W)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GPX2(YBR244W)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress Gene:GSY2(YLR258W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GUP1(YGL084C)|FD-Score:4.43|P-value:4.79E-6||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:IRS4(YKR019C)|FD-Score:8.03|P-value:4.83E-16||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KNS1(YLL019C)|FD-Score:-3.77|P-value:8.10E-5||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LST7(YGR057C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAC1(YMR021C)|FD-Score:6.27|P-value:1.77E-10||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET18(YIL128W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MOT3(YMR070W)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRS2(YOR334W)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MSA1(YOR066W)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:NBP2(YDR162C)|FD-Score:4.38|P-value:5.81E-6||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NUM1(YDR150W)|FD-Score:5.81|P-value:3.12E-9||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:ORM1(YGR038W)|FD-Score:-3.19|P-value:7.09E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:OST6(YML019W)|FD-Score:4.31|P-value:8.35E-6||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PAD1(YDR538W)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PER33(YLR064W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PKR1(YMR123W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:4.85|P-value:6.31E-7||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PSY2(YNL201C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PUS4(YNL292W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:REV7(YIL139C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGP1(YDR137W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM21(YNL294C)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPE1(YJL121C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL24A(YGL031C)|FD-Score:5|P-value:2.91E-7||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS21B(YJL136C)|FD-Score:5.05|P-value:2.21E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:8.19|P-value:1.27E-16||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RTT107(YHR154W)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SER3(YER081W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SFT2(YBL102W)|FD-Score:5.43|P-value:2.80E-8||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SIN3(YOL004W)|FD-Score:4.91|P-value:4.62E-7||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SIT4(YDL047W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKN7(YHR206W)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLG1(YOR008C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNT309(YPR101W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOD1(YJR104C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SQS1(YNL224C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SRB5(YGR104C)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SUR4(YLR372W)|FD-Score:8.55|P-value:6.14E-18||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYS1(YJL004C)|FD-Score:9.05|P-value:7.15E-20||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TLG2(YOL018C)|FD-Score:6.62|P-value:1.79E-11||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TSA1(YML028W)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:10.4|P-value:1.00E-25||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBR2(YLR024C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:UPS2(YLR168C)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:URC2(YDR520C_p)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VPS1(YKR001C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:XRS2(YDR369C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YAP1(YML007W)|FD-Score:8.69|P-value:1.88E-18||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YAT2(YER024W)|FD-Score:-4.49|P-value:3.57E-6||SGD DESC:Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane Gene:YDR003W-A(YDR003W-A_p)|FD-Score:4.4|P-value:5.33E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR095C(YDR095C_d)|FD-Score:3.9|P-value:4.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR476C(YDR476C_p)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YDR535C(YDR535C_d)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEL1(YBL060W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER077C(YER077C_p)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGR079W(YGR079W_p)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YIA6(YIL006W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL002W-A(YIL002W-A_p)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL147C(YJL147C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKL202W(YKL202W_d)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR283W(YLR283W_p)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YNL203C(YNL203C_d)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL099C(YOL099C_d)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR077C(YPR077C_d)|FD-Score:5.76|P-value:4.31E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR123C(YPR123C_d)|FD-Score:4.37|P-value:6.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR148C(YPR148C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR190W_d5.601.05E-81.38YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YOR310C5.482.16E-81.38NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YDR240C4.102.08E-50.07SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YBR011C4.032.81E-50.03IPP1Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
YPL131W3.993.26E-50.16RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YKR038C3.846.24E-50.32KAE1Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription
YLR355C3.512.22E-40.06ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YOR168W3.462.75E-40.16GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YMR309C3.304.89E-40.01NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YNL110C3.295.04E-40.16NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YJL008C3.138.86E-40.11CCT8Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YKL095W3.020.001280.08YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing
YLR147C2.940.001660.01SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
YIL142W2.930.001700.05CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YKL004W2.880.001960.00AUR1Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL005C13.405.78E-41COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YML071C13.005.88E-39COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR058C-A10.401.00E-25TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YJL004C9.057.15E-20SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YML007W8.691.88E-18YAP1Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YLR372W8.556.14E-18SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YJL047C8.191.27E-16RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
YKR019C8.034.83E-16IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YLR094C7.921.22E-15GIS3Protein of unknown function
YOL018C6.621.79E-11TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YMR021C6.271.77E-10MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YNL041C6.183.19E-10COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR150W5.813.12E-9NUM1Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
YOR002W5.783.63E-9ALG6Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
YPR077C_d5.764.31E-9YPR077C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1

GO enrichment analysis for SGTC_1078
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2804.16E-107SGTC_2740felodipine 17.1 μMMiscellaneous33330.107692
0.2492.19E-84SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0617284Golgi
0.2398.66E-78SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0166667Golgi
0.2329.84E-73SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.027027Golgi
0.2265.43E-69SGTC_7171532-0246 73.2 μMChemDiv (Drug-like library)95619920.0588235Golgi
0.2247.90E-68SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.0454545Golgi
0.2233.23E-67SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.0350877Golgi
0.2182.13E-64SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0266667Golgi
0.2183.45E-64SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.0909091Golgi
0.2162.84E-63SGTC_980074-0023 107.7 μMChemDiv (Drug-like library)35638210.150943Golgi
0.2145.31E-62SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0547945TSC3-RPN4
0.2123.84E-61SGTC_33259138760 55.2 μMChembridge (Drug-like library)438449590.0675676Golgi
0.2107.06E-60SGTC_1080nisoldipine 24.4 μMMiscellaneous44990.1TSC3-RPN4
0.2033.75E-56SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.056338Golgi
0.2022.55E-55SGTC_2552juarezic acid 87.0 μMMicrosource (Natural product library)15495120.06TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_495decylubiquinone155 μM0.7272732971ICCB bioactive library322.43915.06204copper-dependent oxidative stress
SGTC_470aa-861153 μM0.4047621967ICCB bioactive library326.429345.75713sphingolipid biosynthesis & PDR1
SGTC_1901st06021658.6 μM0.229167688792TimTec (Natural product derivative library)204.221882.5203
SGTC_26133',4'-dimethoxydalbergione24.65 μM0.207547115019Microsource (Natural product library)284.306542.31504
SGTC_1864st05764530.4 μM0.188679688666TimTec (Natural product derivative library)282.290662.80804amide catabolism
SGTC_2828799886171.43 μM0.1864412988188Chembridge (Drug-like library)325.358463.0404
SGTC_1816st05195791.6 μM0.183673674121TimTec (Natural product derivative library)218.248463.26203
SGTC_820054-0107293.76 μM0.1818183104697ChemDiv (Drug-like library)340.412866.11514
SGTC_18693',7'-dimethoxyflavone40.3 μM0.181818688664TimTec (Natural product derivative library)282.290662.80804
SGTC_25623',7'-dimethoxyflavone64.92 μM0.181818688664Microsource (Natural product library)282.290662.80804
SGTC_1865st05763952.2 μM0.178571688818TimTec (Natural product derivative library)312.316642.79105