nisoldipine FDA approved compound

3-O-methyl 5-O-(2-methylpropyl) 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate

Nisoldipine (INN) is an L-type calcium channel blocker of the dihydropyridine class.

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PubChem MeSH terms: Antihypertensive Agents;Calcium Channel Blockers;Vasodilator Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1080
Screen concentration 24.4 μM
Source Miscellaneous
PubChem CID 4499
SMILES CC1=C(C(C(=C(N1)C)C(=O)OCC(C)C)C2=CC=CC=C2[N+](=O)[O-])C(=O)OC
Standardized SMILES COC(=C1C(C(=C(C)N=C1C)C(=O)OCC(C)C)c2ccccc2[N+](=O)[O-])O
Molecular weight 388.4144
ALogP 2.96
H-bond donor count 1
H-bond acceptor count 7
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.6
% growth inhibition (Hom. pool) 3.71


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4499
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.03||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:COG2(YGR120C)|FD-Score:-3.15|P-value:8.08E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DED1(YOR204W)|FD-Score:4.57|P-value:2.46E-6|Clearance:0.41||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DIS3(YOL021C)|FD-Score:3.12|P-value:9.07E-4|Clearance:0.03||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:LCB1(YMR296C)|FD-Score:5.36|P-value:4.13E-8|Clearance:0.69||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MMS21(YEL019C)|FD-Score:4.67|P-value:1.48E-6|Clearance:0.11||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:MTR10(YOR160W)|FD-Score:3.16|P-value:7.78E-4|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MVD1(YNR043W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.08||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NOG1(YPL093W)|FD-Score:-4.4|P-value:5.44E-6|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP58(YOR310C)|FD-Score:3.46|P-value:2.65E-4|Clearance:0.11||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PSF2(YJL072C)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.04||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PSF3(YOL146W)|FD-Score:3.29|P-value:5.02E-4|Clearance:0.13||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RFC1(YOR217W)|FD-Score:4.03|P-value:2.80E-5|Clearance:0.39||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:TAF4(YMR005W)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.13||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TFC8(YPL007C)|FD-Score:-3.35|P-value:4.11E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:UTP6(YDR449C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.1||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:WRS1(YOL097C)|FD-Score:3.35|P-value:3.99E-4|Clearance:0.06||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:ARP2(YDL029W)|FD-Score:3.09|P-value:9.93E-4|Clearance:0.03||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:COG2(YGR120C)|FD-Score:-3.15|P-value:8.08E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DED1(YOR204W)|FD-Score:4.57|P-value:2.46E-6|Clearance:0.41||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DIS3(YOL021C)|FD-Score:3.12|P-value:9.07E-4|Clearance:0.03||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:LCB1(YMR296C)|FD-Score:5.36|P-value:4.13E-8|Clearance:0.69||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MMS21(YEL019C)|FD-Score:4.67|P-value:1.48E-6|Clearance:0.11||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:MTR10(YOR160W)|FD-Score:3.16|P-value:7.78E-4|Clearance:0||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MVD1(YNR043W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.08||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NOG1(YPL093W)|FD-Score:-4.4|P-value:5.44E-6|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP58(YOR310C)|FD-Score:3.46|P-value:2.65E-4|Clearance:0.11||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PSF2(YJL072C)|FD-Score:3.16|P-value:7.87E-4|Clearance:0.04||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PSF3(YOL146W)|FD-Score:3.29|P-value:5.02E-4|Clearance:0.13||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RFC1(YOR217W)|FD-Score:4.03|P-value:2.80E-5|Clearance:0.39||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:TAF4(YMR005W)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.13||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TFC8(YPL007C)|FD-Score:-3.35|P-value:4.11E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:UTP6(YDR449C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.1||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:WRS1(YOL097C)|FD-Score:3.35|P-value:3.99E-4|Clearance:0.06||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4499
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:4.87|P-value:5.64E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AKR2(YOR034C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:APS3(YJL024C)|FD-Score:4.79|P-value:8.43E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARG82(YDR173C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ART10(YLR392C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:BGL2(YGR282C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BNI1(YNL271C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRP1(YGL007W_d)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CNM67(YNL225C)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:6.02|P-value:8.65E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.22|P-value:9.00E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ELG1(YOR144C)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:ERP2(YAL007C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FMP23(YBR047W_p)|FD-Score:4.26|P-value:1.05E-5||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP27(YLR454W_p)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FTR1(YER145C)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GET2(YER083C)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HMS1(YOR032C)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:IDH2(YOR136W)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IMD3(YLR432W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IRC23(YOR044W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:8.07|P-value:3.57E-16||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KKQ8(YKL168C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:MCM16(YPR046W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:NCS6(YGL211W)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NTH1(YDR001C)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:PUT3(YKL015W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:RAD14(YMR201C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RGP1(YDR137W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL11B(YGR085C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SER3(YER081W)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:5.28|P-value:6.45E-8||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SRS2(YJL092W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SSU1(YPL092W)|FD-Score:4.34|P-value:6.99E-6||SGD DESC:Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein Gene:TDA8(YAL064C-A_p)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TIM11(YDR322C-A)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TRE1(YPL176C)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM10(YOL093W)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:12.7|P-value:2.80E-37||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA1(YDL185W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA7(YGR020C)|FD-Score:6.21|P-value:2.65E-10||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH2(YKL119C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:YCP4(YCR004C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR050C(YCR050C_p)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL057W(YDL057W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YDR535C(YDR535C_d)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER121W(YER121W_p)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YHR180W(YHR180W_d)|FD-Score:6.32|P-value:1.28E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL025C(YIL025C_d)|FD-Score:4.31|P-value:8.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIP4(YGL198W)|FD-Score:-4.31|P-value:8.09E-6||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKR005C(YKR005C_p)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function Gene:YLR294C(YLR294C_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YLR416C(YLR416C_d)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR253C(YMR253C_p)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL211C(YNL211C_p)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YNR048W(YNR048W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOP1(YPR028W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR200W(YOR200W_d)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YOR263C(YOR263C_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YOR292C(YOR292C_p)|FD-Score:4.32|P-value:7.73E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:YPL034W(YPL034W_p)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL182C(YPL182C_d)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR015C(YPR015C_p)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YTP1(YNL237W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:AFT1(YGL071W)|FD-Score:4.87|P-value:5.64E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AKR2(YOR034C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication Gene:APS3(YJL024C)|FD-Score:4.79|P-value:8.43E-7||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARG82(YDR173C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ART10(YLR392C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:BGL2(YGR282C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:BNI1(YNL271C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRP1(YGL007W_d)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CNM67(YNL225C)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:6.02|P-value:8.65E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.22|P-value:9.00E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ELG1(YOR144C)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:ERP2(YAL007C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:FMP23(YBR047W_p)|FD-Score:4.26|P-value:1.05E-5||SGD DESC:Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP27(YLR454W_p)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FTR1(YER145C)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GET2(YER083C)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HMS1(YOR032C)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:IDH2(YOR136W)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IMD3(YLR432W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IRC23(YOR044W)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:8.07|P-value:3.57E-16||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KKQ8(YKL168C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:MCM16(YPR046W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:NCS6(YGL211W)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NTH1(YDR001C)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:PUT3(YKL015W)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:RAD14(YMR201C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RGP1(YDR137W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPL11B(YGR085C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RPS27A(YKL156W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SEC66(YBR171W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SER3(YER081W)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:5.28|P-value:6.45E-8||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SRS2(YJL092W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SSU1(YPL092W)|FD-Score:4.34|P-value:6.99E-6||SGD DESC:Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein Gene:TDA8(YAL064C-A_p)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TIM11(YDR322C-A)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TRE1(YPL176C)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM10(YOL093W)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:12.7|P-value:2.80E-37||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA1(YDL185W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA7(YGR020C)|FD-Score:6.21|P-value:2.65E-10||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH2(YKL119C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:YCP4(YCR004C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR050C(YCR050C_p)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL057W(YDL057W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YDR535C(YDR535C_d)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER121W(YER121W_p)|FD-Score:3.82|P-value:6.65E-5||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YHR180W(YHR180W_d)|FD-Score:6.32|P-value:1.28E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL025C(YIL025C_d)|FD-Score:4.31|P-value:8.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIP4(YGL198W)|FD-Score:-4.31|P-value:8.09E-6||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKR005C(YKR005C_p)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function Gene:YLR294C(YLR294C_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YLR416C(YLR416C_d)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR253C(YMR253C_p)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL211C(YNL211C_p)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YNR048W(YNR048W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOP1(YPR028W)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR200W(YOR200W_d)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YOR263C(YOR263C_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YOR292C(YOR292C_p)|FD-Score:4.32|P-value:7.73E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:YPL034W(YPL034W_p)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL182C(YPL182C_d)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR015C(YPR015C_p)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YTP1(YNL237W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR296C5.364.13E-80.69LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YEL019C4.671.48E-60.10MMS21SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
YOR204W4.572.46E-60.41DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YMR005W4.161.60E-50.13TAF4TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
YOR217W4.032.80E-50.39RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YNR043W3.641.37E-40.08MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YDR449C3.561.84E-40.10UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YOR310C3.462.65E-40.11NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YOL097C3.353.99E-40.06WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YOL146W3.295.02E-40.12PSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YOR160W3.167.78E-40.00MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YJL072C3.167.87E-40.04PSF2Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YOL021C3.129.07E-40.03DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YDL029W3.099.93E-40.03ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YGR251W3.060.001110.01NOP19Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A12.702.80E-37TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YKR019C8.073.57E-16IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YHR180W_d6.321.28E-10YHR180W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGR020C6.212.65E-10VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YGL005C6.028.65E-10COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBL007C5.286.45E-8SLA1Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
YML071C5.229.00E-8COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YOR136W5.181.09E-7IDH2Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated
YER145C5.062.14E-7FTR1High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
YGL071W4.875.64E-7AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YJL024C4.798.43E-7APS3Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
YPR015C_p4.721.17E-6YPR015C_pPutative protein of unknown function; overexpression causes a cell cycle delay or arrest
YMR201C4.621.93E-6RAD14Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
YDR137W4.582.33E-6RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YNL041C4.533.01E-6COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments

GO enrichment analysis for SGTC_1080
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2691.96E-98SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.116279TSC3-RPN4
0.2682.10E-97SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0394737TSC3-RPN4
0.2525.73E-86SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.08TSC3-RPN4
0.2493.23E-84SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.109589TSC3-RPN4
0.2497.49E-84SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0722892TSC3-RPN4
0.2417.54E-79SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.2TSC3-RPN4
0.2391.15E-77SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.115385TSC3-RPN4
0.2341.77E-74SGTC_2740felodipine 17.1 μMMiscellaneous33330.491803
0.2326.19E-73SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0972222TSC3-RPN4
0.2301.79E-71SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.19697TSC3-RPN4
0.2296.99E-71SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.084507TSC3-RPN4
0.2221.95E-66SGTC_2552juarezic acid 87.0 μMMicrosource (Natural product library)15495120.125TSC3-RPN4
0.2214.32E-66SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0821918TSC3-RPN4
0.2155.52E-63SGTC_1815st051655 58.2 μMTimTec (Natural product derivative library)59841560.103448TSC3-RPN4
0.2142.87E-62SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.15493TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_481bay k-864450 μM0.5161292303ICCB bioactive library356.296512.67318
SGTC_488nitrendipine139 μM0.5079374507Miscellaneous360.36122.11517
SGTC_2740felodipine17.08 μM0.4918033333Miscellaneous384.253763.54915
SGTC_487nimodipine119 μM0.4428574497Miscellaneous418.440342.36118
SGTC_1988st07187253.4 μM0.42465816414116TimTec (Natural product derivative library)374.301660.19729
SGTC_486niguldipine82 μM0.4074071236Miscellaneous609.711365.40718amide catabolism
SGTC_2712st07912647.7 μM0.3378383152970TimTec (Natural product derivative library)419.426642.51617
SGTC_12390337-058435.4 μM0.3914301ChemDiv (Drug-like library)318.077622.18306unfolded protein response
SGTC_1107amlodipine47 μM0.2771082162NIH Clinical Collection408.87591.57627
SGTC_448monastrol171 μM0.2739732987927ICCB bioactive library292.353442.45934