sertraline

(1S,4S)-4-(3,4-dichlorophenyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine hydrochloride

A selective serotonin uptake inhibitor that is used in the treatment of depression. Also called zoloft.

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PubChem MeSH terms: Antidepressive Agents;Serotonin Uptake Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1081
Screen concentration 6.0 μM
Source NIH Clinical Collection
PubChem CID 63009
SMILES CNC1CCC(C2=CC=CC=C12)C3=CC(=C(C=C3)Cl)Cl.Cl
Standardized SMILES CNC1CCC(c2ccc(Cl)c(Cl)c2)c3ccccc13
Molecular weight 342.6905
ALogP 5.38
H-bond donor count 2
H-bond acceptor count 1
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.97
% growth inhibition (Hom. pool) 9.88


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 63009
Download HIP data (tab-delimited text)  (excel)
Gene:APC2(YLR127C)|FD-Score:4.02|P-value:2.90E-5|Clearance:0.13||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:CCT6(YDR188W)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC31(YOR257W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.04||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:IRA1(YBR140C)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:16.5|P-value:1.10E-61|Clearance:11.21||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM3(YLR438C-A)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NMD3(YHR170W)|FD-Score:5.32|P-value:5.08E-8|Clearance:0.44||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP9(YJL010C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.2||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PSF3(YOL146W)|FD-Score:3.89|P-value:5.02E-5|Clearance:0.08||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPB11(YOL005C)|FD-Score:-3.39|P-value:3.47E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPS3(YNL178W)|FD-Score:-3.11|P-value:9.29E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP36(YOR287C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.04||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:RRP9(YPR137W)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSP5(YER125W)|FD-Score:4.88|P-value:5.21E-7|Clearance:0.64||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SEC53(YFL045C)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.02||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SEN1(YLR430W)|FD-Score:-3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SNU114(YKL173W)|FD-Score:3.81|P-value:7.02E-5|Clearance:0.36||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:YJL009W(YJL009W_d)|FD-Score:-3.61|P-value:1.53E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YTM1(YOR272W)|FD-Score:3.3|P-value:4.77E-4|Clearance:0.19||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:APC2(YLR127C)|FD-Score:4.02|P-value:2.90E-5|Clearance:0.13||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:CCT6(YDR188W)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC31(YOR257W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.04||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:IRA1(YBR140C)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:16.5|P-value:1.10E-61|Clearance:11.21||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM3(YLR438C-A)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.1||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NMD3(YHR170W)|FD-Score:5.32|P-value:5.08E-8|Clearance:0.44||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP9(YJL010C)|FD-Score:4.23|P-value:1.19E-5|Clearance:0.2||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PSF3(YOL146W)|FD-Score:3.89|P-value:5.02E-5|Clearance:0.08||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPB11(YOL005C)|FD-Score:-3.39|P-value:3.47E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPS3(YNL178W)|FD-Score:-3.11|P-value:9.29E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP36(YOR287C)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.04||SGD DESC:Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit Gene:RRP9(YPR137W)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSP5(YER125W)|FD-Score:4.88|P-value:5.21E-7|Clearance:0.64||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SEC53(YFL045C)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.02||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SEN1(YLR430W)|FD-Score:-3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SNU114(YKL173W)|FD-Score:3.81|P-value:7.02E-5|Clearance:0.36||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:YJL009W(YJL009W_d)|FD-Score:-3.61|P-value:1.53E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YTM1(YOR272W)|FD-Score:3.3|P-value:4.77E-4|Clearance:0.19||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 63009
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALG6(YOR002W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARG3(YJL088W)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:BCK1(YJL095W)|FD-Score:8.7|P-value:1.60E-18||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CDC50(YCR094W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:DEP1(YAL013W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:END3(YNL084C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERI1(YPL096C-A)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:ERV14(YGL054C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAR11(YNL127W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GDB1(YPR184W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GTR2(YGR163W)|FD-Score:5.51|P-value:1.79E-8||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HEM14(YER014W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HOG1(YLR113W)|FD-Score:-4.32|P-value:7.74E-6||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:IES3(YLR052W)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:JHD2(YJR119C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:MEI4(YER044C-A)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MLS1(YNL117W)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MOD5(YOR274W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRP4(YHL004W)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAB6(YML117W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NHX1(YDR456W)|FD-Score:8.97|P-value:1.42E-19||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NMD2(YHR077C)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:OCA2(YNL056W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OMA1(YKR087C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PAN3(YKL025C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDA1(YER178W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PHA2(YNL316C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PIN4(YBL051C)|FD-Score:6.07|P-value:6.57E-10||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POP2(YNR052C)|FD-Score:5.07|P-value:1.94E-7||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM5(YIL117C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PSK2(YOL045W)|FD-Score:7.91|P-value:1.26E-15||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:10.3|P-value:3.12E-25||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RDS1(YCR106W)|FD-Score:4.89|P-value:5.16E-7||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REG1(YDR028C)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REI1(YBR267W)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RGP1(YDR137W)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPP1B(YDL130W)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RRP8(YDR083W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RXT2(YBR095C)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:RXT3(YDL076C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAM37(YMR060C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SEA4(YBL104C)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SHR5(YOL110W)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SIS2(YKR072C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKI8(YGL213C)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SLG1(YOR008C)|FD-Score:5.21|P-value:9.69E-8||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:16.8|P-value:1.70E-63||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNQ2(YDR011W)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SOD1(YJR104C)|FD-Score:4.84|P-value:6.61E-7||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE3(YPR069C)|FD-Score:4.29|P-value:8.84E-6||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPF1(YEL031W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO74(YGL170C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SRB8(YCR081W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSO1(YPL232W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SUM1(YDR310C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SVS1(YPL163C)|FD-Score:4.4|P-value:5.38E-6||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TRE1(YPL176C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBC12(YLR306W)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UGA2(YBR006W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VAC7(YNL054W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS51(YKR020W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAP1802(YGR241C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YAP3(YHL009C)|FD-Score:6.15|P-value:3.96E-10||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL071C(YBL071C_d)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR027C(YBR027C_d)|FD-Score:5.29|P-value:6.16E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:7.72|P-value:5.75E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER130C(YER130C_p)|FD-Score:4.28|P-value:9.18E-6||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL159W(YGL159W_p)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHR125W(YHR125W_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR460C(YLR460C_p)|FD-Score:6.54|P-value:3.05E-11||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YNL170W(YNL170W_d)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function Gene:YNL319W(YNL319W_d)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YPR039W(YPR039W_d)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR197C(YPR197C_d)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:9.65|P-value:2.38E-22||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALG6(YOR002W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARG3(YJL088W)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:BCK1(YJL095W)|FD-Score:8.7|P-value:1.60E-18||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CDC50(YCR094W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:DEP1(YAL013W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:END3(YNL084C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERI1(YPL096C-A)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:ERV14(YGL054C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAR11(YNL127W)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GDB1(YPR184W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GTR2(YGR163W)|FD-Score:5.51|P-value:1.79E-8||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HEM14(YER014W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HOG1(YLR113W)|FD-Score:-4.32|P-value:7.74E-6||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:IES3(YLR052W)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:JHD2(YJR119C)|FD-Score:3.97|P-value:3.59E-5||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:MEI4(YER044C-A)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MLS1(YNL117W)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MOD5(YOR274W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRP4(YHL004W)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAB6(YML117W)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NHX1(YDR456W)|FD-Score:8.97|P-value:1.42E-19||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NMD2(YHR077C)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance Gene:OCA2(YNL056W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OMA1(YKR087C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:PAN3(YKL025C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDA1(YER178W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PHA2(YNL316C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PIN4(YBL051C)|FD-Score:6.07|P-value:6.57E-10||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POP2(YNR052C)|FD-Score:5.07|P-value:1.94E-7||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM5(YIL117C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PSK2(YOL045W)|FD-Score:7.91|P-value:1.26E-15||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:10.3|P-value:3.12E-25||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RDS1(YCR106W)|FD-Score:4.89|P-value:5.16E-7||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REG1(YDR028C)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REI1(YBR267W)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RGP1(YDR137W)|FD-Score:-4.26|P-value:1.04E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPP1B(YDL130W)|FD-Score:-3.57|P-value:1.75E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RRP8(YDR083W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RXT2(YBR095C)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:RXT3(YDL076C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAM37(YMR060C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SEA4(YBL104C)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SHR5(YOL110W)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SIS2(YKR072C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKI8(YGL213C)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SLG1(YOR008C)|FD-Score:5.21|P-value:9.69E-8||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:16.8|P-value:1.70E-63||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNQ2(YDR011W)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SOD1(YJR104C)|FD-Score:4.84|P-value:6.61E-7||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE3(YPR069C)|FD-Score:4.29|P-value:8.84E-6||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPF1(YEL031W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO74(YGL170C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SRB8(YCR081W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSO1(YPL232W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SUM1(YDR310C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SVS1(YPL163C)|FD-Score:4.4|P-value:5.38E-6||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TRE1(YPL176C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBC12(YLR306W)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Enzyme that mediates the conjugation of Rub1p, a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes Gene:UGA2(YBR006W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VAC7(YNL054W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS51(YKR020W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAP1802(YGR241C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YAP3(YHL009C)|FD-Score:6.15|P-value:3.96E-10||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL071C(YBL071C_d)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR027C(YBR027C_d)|FD-Score:5.29|P-value:6.16E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:7.72|P-value:5.75E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER130C(YER130C_p)|FD-Score:4.28|P-value:9.18E-6||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL159W(YGL159W_p)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YHR125W(YHR125W_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR460C(YLR460C_p)|FD-Score:6.54|P-value:3.05E-11||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YNL170W(YNL170W_d)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL277W-A(YNL277W-A_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function Gene:YNL319W(YNL319W_d)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YPR039W(YPR039W_d)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR197C(YPR197C_d)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT31(YER031C)|FD-Score:9.65|P-value:2.38E-22||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W16.501.10E-6111.20LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W5.325.08E-80.44NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YER125W4.885.21E-70.64RSP5E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p
YFL045C4.251.08E-50.02SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YJL010C4.231.19E-50.20NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YLR127C4.022.90E-50.13APC2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
YOL146W3.895.02E-50.08PSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YKL173W3.817.02E-50.36SNU114GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
YLR438C-A3.442.87E-40.10LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YOR257W3.344.17E-40.04CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YOR272W3.304.77E-40.19YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YOR287C3.129.16E-40.04RRP36Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
YIR006C3.080.001040.15PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YOR361C2.930.001700.17PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YOR217W2.760.002870.08RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR030C16.801.70E-63SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YJL204C10.303.12E-25RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YER031C9.652.38E-22YPT31Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
YDR456W8.971.42E-19NHX1Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
YJL095W8.701.60E-18BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YOL045W7.911.26E-15PSK2PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication
YDR455C_d7.725.75E-15YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YLR460C_p6.543.05E-11YLR460C_pMember of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine
YHL009C6.153.96E-10YAP3Basic leucine zipper (bZIP) transcription factor
YBL051C6.076.57E-10PIN4Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
YHR077C5.822.91E-9NMD2Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance
YGR163W5.511.79E-8GTR2Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
YBR027C_d5.296.16E-8YBR027C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOR008C5.219.69E-8SLG1Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
YBL104C5.091.76E-7SEA4Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites

GO enrichment analysis for SGTC_1081
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3303.35E-150SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.76315860S ribosome export
0.2994.98E-122SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.196078cell wall signaling
0.2653.37E-95SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.0925926cell wall signaling
0.2483.66E-83SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.14cell wall signaling
0.2429.40E-80SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.090909160S ribosome export
0.2372.27E-76SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.11764760S ribosome export
0.2362.73E-75SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0769231cell wall signaling
0.2295.48E-71SGTC_500h-89 112.0 μMICCB bioactive library4492410.0632911cell wall signaling
0.2271.48E-69SGTC_1811447-1644 8.1 μMChemDiv (Drug-like library)5346000.121622endomembrane recycling
0.2271.30E-69SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.069767460S ribosome export
0.2221.18E-66SGTC_31009120536 49.5 μMChembridge (Drug-like library)164211960.0833333
0.2214.86E-66SGTC_29249010168 62.8 μMChembridge (Drug-like library)29939290.0895522cell wall signaling
0.2192.79E-65SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.0857143endomembrane recycling
0.2189.07E-65SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.106667cell wall signaling
0.2076.48E-58SGTC_3690957-0393 4.5 μMChemDiv (Drug-like library)69979920.0574713cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1070indatraline8.81 μM0.76315810314472NIH Clinical Collection328.663944.9222160S ribosome export
SGTC_1823448-4371550.52 μM0.2950823491403ChemDiv (Drug-like library)363.28095.42211
SGTC_2724maprotiline69.64 μM0.2592594011Miscellaneous277.403324.11611ERG2
SGTC_344k064-00356.43 μM0.2549025088750ChemDiv (Drug-like library)339.217986.2321260S ribosome export
SGTC_7211187-1332279 μM0.25490225730ChemDiv (Drug-like library)281.13733.96522cell wall signaling
SGTC_3265913726649.47 μM0.25423717224265Chembridge (Drug-like library)357.274783.71112
SGTC_1270180-033359.13 μM0.245614771601ChemDiv (Drug-like library)324.158743.71113mitochondrial response to ROS
SGTC_12971068-011114.1 μM0.236364767057ChemDiv (Drug-like library)312.214244.33712
SGTC_12961068-010211.9 μM0.22807767053ChemDiv (Drug-like library)310.175224.02712
SGTC_2929901334824.6 μM0.228072995952Chembridge (Drug-like library)310.175223.99912