voriconazole FDA approved compound

(2R,3S)-2-(2,4-difluorophenyl)-3-(5-fluoropyrimidin-4-yl)-1-(1,2,4-triazol-1-yl)butan-2-ol


Established Yeast Drug Target : ERG11

Voriconazole is an antifungal that acts by inhibiting lanosterol 14α-demethylase, thereby preventing the conversion of lanosterol to ergosterol, an essential component of the fungal cytoplasmic membrane.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1084
Screen concentration 435.0 nM
Source NIH Clinical Collection
PubChem CID 71616
SMILES CC(C1=NC=NC=C1F)C(CN2C=NC=N2)(C3=C(C=C(C=C3)F)F)O
Standardized SMILES CC(c1ncncc1F)C(O)(Cn2cncn2)c3ccc(F)cc3F
Molecular weight 349.3105
ALogP 2.07
H-bond donor count 1
H-bond acceptor count 8
Response signature azole & statin

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.35
% growth inhibition (Hom. pool) 3.71


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 71616
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:7.68|P-value:8.13E-15|Clearance:1.56||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP7(YPR034W)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC53(YDL132W)|FD-Score:-3.18|P-value:7.37E-4|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CIA2(YHR122W)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.08||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:DML1(YMR211W)|FD-Score:5.75|P-value:4.56E-9|Clearance:0.87||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:6.12|P-value:4.68E-10|Clearance:0.37||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.35||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LAS17(YOR181W)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.04||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:MCD4(YKL165C)|FD-Score:3.77|P-value:8.22E-5|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM6(YGL201C)|FD-Score:-3.15|P-value:8.13E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MED4(YOR174W)|FD-Score:3.9|P-value:4.73E-5|Clearance:0.14||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:PAN1(YIR006C)|FD-Score:4.87|P-value:5.54E-7|Clearance:0.41||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:PRP19(YLL036C)|FD-Score:3.77|P-value:8.27E-5|Clearance:0.03||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RPN11(YFR004W)|FD-Score:3.49|P-value:2.45E-4|Clearance:0.04||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SFT1(YKL006C-A)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.17||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:TIF35(YDR429C)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.2||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRS31(YDR472W)|FD-Score:-3.29|P-value:5.02E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TUB1(YML085C)|FD-Score:7.83|P-value:2.46E-15|Clearance:0.15||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YBL077W(YBL077W_d)|FD-Score:4.47|P-value:3.98E-6|Clearance:0.33||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YJL195C(YJL195C_d)|FD-Score:11.6|P-value:2.74E-31|Clearance:3.75||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTM1(YOR272W)|FD-Score:3.94|P-value:4.13E-5|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:ACT1(YFL039C)|FD-Score:7.68|P-value:8.13E-15|Clearance:1.56||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP7(YPR034W)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC53(YDL132W)|FD-Score:-3.18|P-value:7.37E-4|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CIA2(YHR122W)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.08||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:DML1(YMR211W)|FD-Score:5.75|P-value:4.56E-9|Clearance:0.87||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:6.12|P-value:4.68E-10|Clearance:0.37||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.35||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LAS17(YOR181W)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.04||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:MCD4(YKL165C)|FD-Score:3.77|P-value:8.22E-5|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM6(YGL201C)|FD-Score:-3.15|P-value:8.13E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:MED4(YOR174W)|FD-Score:3.9|P-value:4.73E-5|Clearance:0.14||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:PAN1(YIR006C)|FD-Score:4.87|P-value:5.54E-7|Clearance:0.41||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:PRP19(YLL036C)|FD-Score:3.77|P-value:8.27E-5|Clearance:0.03||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RPN11(YFR004W)|FD-Score:3.49|P-value:2.45E-4|Clearance:0.04||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SFT1(YKL006C-A)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.17||SGD DESC:Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment Gene:TIF35(YDR429C)|FD-Score:4.13|P-value:1.79E-5|Clearance:0.2||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRS31(YDR472W)|FD-Score:-3.29|P-value:5.02E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TUB1(YML085C)|FD-Score:7.83|P-value:2.46E-15|Clearance:0.15||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YBL077W(YBL077W_d)|FD-Score:4.47|P-value:3.98E-6|Clearance:0.33||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YJL195C(YJL195C_d)|FD-Score:11.6|P-value:2.74E-31|Clearance:3.75||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YTM1(YOR272W)|FD-Score:3.94|P-value:4.13E-5|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 71616
Download HOP data (tab-delimited text)  (excel)
Gene:AIM29(YKR074W)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARL3(YPL051W)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BMH1(YER177W)|FD-Score:5.75|P-value:4.52E-9||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BST1(YFL025C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CHS5(YLR330W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:COG7(YGL005C)|FD-Score:-7.1|P-value:6.40E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL5(YJR152W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DAP1(YPL170W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DBP7(YKR024C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DEP1(YAL013W)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DFG16(YOR030W)|FD-Score:4.28|P-value:9.15E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:EDS1(YBR033W_p)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMC5(YIL027C)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FIN1(YDR130C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FYV1(YDR024W_d)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GCN3(YKR026C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GPB1(YOR371C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GTR2(YGR163W)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HDA3(YPR179C)|FD-Score:5.35|P-value:4.42E-8||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HMG1(YML075C)|FD-Score:8.15|P-value:1.82E-16||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HXT12(YIL170W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:IFM1(YOL023W)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRC2(YDR112W_d)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRE1(YHR079C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LNP1(YHR192W_p)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MNT3(YIL014W)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:NOP12(YOL041C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OPI3(YJR073C)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH3(YHR073W)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDR1(YGL013C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PMP1(YCR024C-A)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RIM101(YHL027W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:8.33|P-value:3.91E-17||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM20(YOR275C)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM8(YGL045W)|FD-Score:5.06|P-value:2.15E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:7.38|P-value:8.15E-14||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RMT2(YDR465C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL4A(YBR031W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC7(YDR389W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAM3(YPL274W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SNF7(YLR025W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNG1(YGR197C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:SOP4(YJL192C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPT8(YLR055C)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SSO1(YPL232W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SWD3(YBR175W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TIS11(YLR136C)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TPN1(YGL186C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM7(YBR061C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP3(YKL211C)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UTR1(YJR049C)|FD-Score:8.21|P-value:1.14E-16||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VMA3(YEL027W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS25(YJR102C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS5(YOR069W)|FD-Score:6.05|P-value:7.23E-10||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS9(YML097C)|FD-Score:5.35|P-value:4.43E-8||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YDL118W(YDL118W_p)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YGR122W(YGR122W)|FD-Score:8.78|P-value:7.86E-19||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHP1(YDR451C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR045W(YHR045W_p)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YKR033C(YKR033C_d)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLR281C(YLR281C_p)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YNL235C(YNL235C_d)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL153C(YOL153C)|FD-Score:3.73|P-value:9.44E-5||SGD DESC:Hypothetical protein Gene:YPK1(YKL126W)|FD-Score:8.88|P-value:3.35E-19||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:AIM29(YKR074W)|FD-Score:4.75|P-value:1.00E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARL3(YPL051W)|FD-Score:-3.9|P-value:4.81E-5||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:BMH1(YER177W)|FD-Score:5.75|P-value:4.52E-9||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BST1(YFL025C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CHS5(YLR330W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:COG7(YGL005C)|FD-Score:-7.1|P-value:6.40E-13||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL5(YJR152W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DAP1(YPL170W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DBP7(YKR024C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DEP1(YAL013W)|FD-Score:3.75|P-value:8.95E-5||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DFG16(YOR030W)|FD-Score:4.28|P-value:9.15E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:EDS1(YBR033W_p)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMC5(YIL027C)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FIN1(YDR130C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FYV1(YDR024W_d)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GCN3(YKR026C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GPB1(YOR371C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GTR2(YGR163W)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HDA3(YPR179C)|FD-Score:5.35|P-value:4.42E-8||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HMG1(YML075C)|FD-Score:8.15|P-value:1.82E-16||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HXT12(YIL170W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family Gene:IFM1(YOL023W)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRC2(YDR112W_d)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRE1(YHR079C)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LNP1(YHR192W_p)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MNT3(YIL014W)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:NOP12(YOL041C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OPI3(YJR073C)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OSH3(YHR073W)|FD-Score:4.27|P-value:9.81E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDR1(YGL013C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PMP1(YCR024C-A)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:RIM101(YHL027W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:8.33|P-value:3.91E-17||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM20(YOR275C)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM8(YGL045W)|FD-Score:5.06|P-value:2.15E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:7.38|P-value:8.15E-14||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RMT2(YDR465C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL4A(YBR031W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC7(YDR389W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAM3(YPL274W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SNF7(YLR025W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNG1(YGR197C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:SOP4(YJL192C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPT8(YLR055C)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SSO1(YPL232W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SWD3(YBR175W)|FD-Score:3.17|P-value:7.74E-4||SGD DESC:Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 Gene:TIS11(YLR136C)|FD-Score:3.81|P-value:6.84E-5||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TPN1(YGL186C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM7(YBR061C)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TRP3(YKL211C)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UTR1(YJR049C)|FD-Score:8.21|P-value:1.14E-16||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VMA3(YEL027W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS25(YJR102C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS5(YOR069W)|FD-Score:6.05|P-value:7.23E-10||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS9(YML097C)|FD-Score:5.35|P-value:4.43E-8||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YDL118W(YDL118W_p)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YGR122W(YGR122W)|FD-Score:8.78|P-value:7.86E-19||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHP1(YDR451C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR045W(YHR045W_p)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YKR033C(YKR033C_d)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLR281C(YLR281C_p)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YNL235C(YNL235C_d)|FD-Score:3.73|P-value:9.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL153C(YOL153C)|FD-Score:3.73|P-value:9.44E-5||SGD DESC:Hypothetical protein Gene:YPK1(YKL126W)|FD-Score:8.88|P-value:3.35E-19||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL195C_d11.602.74E-313.75YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YML085C7.832.46E-150.15TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YFL039C7.688.13E-151.56ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YHR007C6.124.68E-100.37ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YMR211W5.754.56E-90.88DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YIR006C4.875.54E-70.41PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YBL077W_d4.473.98E-60.33YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YDR429C4.131.79E-50.20TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YOR272W3.944.13E-50.03YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YOR174W3.904.73E-50.14MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YKL165C3.778.22E-50.00MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YLL036C3.778.27E-50.03PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YOR181W3.749.17E-50.04LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
YPR034W3.711.05E-40.14ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YHR122W3.571.78E-40.08CIA2Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W8.883.35E-19YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YGR122W8.787.86E-19YGR122WProtein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
YMR154C8.333.91E-17RIM13Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
YJR049C8.211.14E-16UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YML075C8.151.82E-16HMG1HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
YMR063W7.388.15E-14RIM9Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
YOR069W6.057.23E-10VPS5Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes
YER177W5.754.52E-9BMH114-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YPR179C5.354.42E-8HDA3Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p
YML097C5.354.43E-8VPS9A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
YDL118W_p5.111.62E-7YDL118W_pDubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
YGL045W5.062.15E-7RIM8Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
YLR055C4.894.93E-7SPT8Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
YKR074W4.751.00E-6AIM29Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss
YJR073C4.671.53E-6OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis

GO enrichment analysis for SGTC_1084
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5140SGTC_227fluconazole 33.5 μMMiscellaneous33650.584906azole & statin
0.5130SGTC_1067clotrimazole 1.4 μMNIH Clinical Collection28120.125azole & statin
0.4571.85E-302SGTC_1788fluconazole 20.0 μMMiscellaneous33650.584906azole & statin
0.4112.77E-239SGTC_2765myclobutanil 7.7 μMMiscellaneous63360.263889azole & statin
0.3253.72E-145SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.135417azole & statin
0.3212.27E-141SGTC_2647atorvastatin 60.2 μMMiscellaneous608230.135417azole & statin
0.2881.45E-113SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.0821918azole & statin
0.2631.11E-93SGTC_5264469-0568 170.0 μMChemDiv (Drug-like library)29019640.0740741azole & statin
0.2416.12E-79SGTC_256clotrimazole 625.0 nMMiscellaneous28120.125ergosterol depletion effects on membrane
0.2411.07E-78SGTC_11911487-1166 10.6 μMChemDiv (Drug-like library)39557710.12azole & statin
0.2406.49E-78SGTC_8620452-0056 10.2 μMChemDiv (Drug-like library)15753960.0909091azole & statin
0.2361.75E-75SGTC_13111190-0148 100.0 μMChemDiv (Drug-like library)19613330.047619azole & statin
0.2145.35E-62SGTC_28167982706 58.4 μMChembridge (Drug-like library)29818530.0909091azole & statin
0.2061.16E-57SGTC_3464513-0042 7.2 μMChemDiv (Drug-like library)28273720.114943azole & statin
0.1951.55E-51SGTC_2778tioconazole 65.1 nMMiscellaneous54820.114943ergosterol depletion effects on membrane

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1787fluconazole80 μM0.5849063365Miscellaneous306.2707860.7517iron homeostasis
SGTC_1788fluconazole20 μM0.5849063365Miscellaneous306.2707860.7517azole & statin
SGTC_227fluconazole33.52 μM0.5849063365Miscellaneous306.2707860.7517azole & statin
SGTC_2765myclobutanil7.74 μM0.2638896336Miscellaneous288.775283.71903azole & statin
SGTC_21485546263113.57 μM0.19403792097Chembridge (Fragment library)216.2541232.76313
SGTC_1734st03716869.4 μM0.177215708907TimTec (Natural product derivative library)288.2770231.68904
SGTC_6641222-005414.9 μM0.1733336813991ChemDiv (Drug-like library)287.3717833.72413sphingolipid biosynthesis & PDR1
SGTC_20605238662140 μM0.171875790130Chembridge (Fragment library)195.1472430.5114
SGTC_1076itraconazole331 nM0.1711713793Miscellaneous705.633426.43409
SGTC_14324049-029868.4 μM0.169014741646ChemDiv (Drug-like library)299.2931462.71216
SGTC_662k832-1615104 μM0.1688315878478ChemDiv (Drug-like library)306.3369032.75712
SGTC_6961560-0013611 μM0.1643841650269ChemDiv (Drug-like library)327.4356436.1913