doxorubicin FDA approved compound

(7S,9S)-7-[(2R,4S,5S,6S)-4-amino-5-hydroxy-6-methyloxan-2-yl]oxy-6,9,11-trihydroxy-9-(2-hydroxyacetyl)-4-methoxy-8,10-dihydro-7H-tetracene-5,12-dione hydrochloride

Doxorubicin forms complexes with DNA by intercalation between base pairs, thereby preventing DNA replication. Additionally, doxorubicin inhibits topoisomerase II activity by stabilizing the DNA-topoisomerase II complex, preventing the religation portion of the topoisomerase II reaction.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1085
Screen concentration 7.7 μM
Source NIH Clinical Collection
PubChem CID 443939
SMILES CC1C(C(CC(O1)OC2CC(CC3=C(C4=C(C(=C23)O)C(=O)C5=C(C4=O)C=CC=C5OC)O)(C(=O)CO)O)N)O.Cl
Standardized SMILES COc1cccc2C(=O)c3c(O)c4CC(O)(CC(OC5CC(N)C(O)C(C)O5)c4c(O)c3C(=O)c12)C(=O)CO
Molecular weight 579.9802
ALogP 0.34
H-bond donor count 7
H-bond acceptor count 12
Response signature anthracycline transcription coupled DNA repair

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 443939
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:4.32|P-value:7.83E-6|Clearance:0.28||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.07||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRN1(YBL097W)|FD-Score:5.18|P-value:1.08E-7|Clearance:0.87||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDS1(YBR029C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.02||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CSE4(YKL049C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.15||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:CUS1(YMR240C)|FD-Score:3.15|P-value:8.26E-4|Clearance:0.04||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:DAD1(YDR016C)|FD-Score:3.2|P-value:6.79E-4|Clearance:0.02||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ESP1(YGR098C)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.07||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:MDN1(YLR106C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.03||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:MED4(YOR174W)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOG1(YPL093W)|FD-Score:-4.42|P-value:4.93E-6|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PAB1(YER165W)|FD-Score:6.24|P-value:2.12E-10|Clearance:1.06||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:RSC8(YFR037C)|FD-Score:4.04|P-value:2.73E-5|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.01|P-value:3.10E-5|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SMD2(YLR275W)|FD-Score:-3.33|P-value:4.29E-4|Clearance:0||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SSL2(YIL143C)|FD-Score:23.1|P-value:6.02E-118|Clearance:16.81||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:TFB2(YPL122C)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.14||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TRZ1(YKR079C)|FD-Score:3.98|P-value:3.38E-5|Clearance:0.23||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:YBL077W(YBL077W_d)|FD-Score:3.16|P-value:7.76E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YEF3(YLR249W)|FD-Score:3.51|P-value:2.28E-4|Clearance:0.03||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:ARP4(YJL081C)|FD-Score:4.32|P-value:7.83E-6|Clearance:0.28||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.07||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRN1(YBL097W)|FD-Score:5.18|P-value:1.08E-7|Clearance:0.87||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDS1(YBR029C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.02||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CSE4(YKL049C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.15||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:CUS1(YMR240C)|FD-Score:3.15|P-value:8.26E-4|Clearance:0.04||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:DAD1(YDR016C)|FD-Score:3.2|P-value:6.79E-4|Clearance:0.02||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ESP1(YGR098C)|FD-Score:3.1|P-value:9.53E-4|Clearance:0.07||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:MDN1(YLR106C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.03||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:MED4(YOR174W)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.13||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOG1(YPL093W)|FD-Score:-4.42|P-value:4.93E-6|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:PAB1(YER165W)|FD-Score:6.24|P-value:2.12E-10|Clearance:1.06||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:RSC8(YFR037C)|FD-Score:4.04|P-value:2.73E-5|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.01|P-value:3.10E-5|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SMD2(YLR275W)|FD-Score:-3.33|P-value:4.29E-4|Clearance:0||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SSL2(YIL143C)|FD-Score:23.1|P-value:6.02E-118|Clearance:16.81||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:TFB2(YPL122C)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.14||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TRZ1(YKR079C)|FD-Score:3.98|P-value:3.38E-5|Clearance:0.23||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:YBL077W(YBL077W_d)|FD-Score:3.16|P-value:7.76E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YEF3(YLR249W)|FD-Score:3.51|P-value:2.28E-4|Clearance:0.03||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 443939
Download HOP data (tab-delimited text)  (excel)
Gene:ADE5,7(YGL234W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AIM25(YJR100C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ASN1(YPR145W)|FD-Score:5.07|P-value:2.04E-7||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BCS1(YDR375C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BDF1(YLR399C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BEM4(YPL161C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BPT1(YLL015W)|FD-Score:3.54|P-value:1.96E-4||SGD DESC:ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p Gene:BRP1(YGL007W_d)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CCM1(YGR150C)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDC10(YCR002C)|FD-Score:3.82|P-value:6.78E-5||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CLD1(YGR110W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CNM67(YNL225C)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CRD1(YDL142C)|FD-Score:5.24|P-value:7.90E-8||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:CSR2(YPR030W)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:DAL1(YIR027C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DOA4(YDR069C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DSF2(YBR007C_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EMC4(YGL231C)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERS1(YCR075C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:EXO1(YOR033C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FAR11(YNL127W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GIT1(YCR098C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:HUL4(YJR036C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:ICE2(YIL090W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IRC23(YOR044W)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IRC5(YFR038W)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:KRE6(YPR159W)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:MNN2(YBR015C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:NCR1(YPL006W)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:PTC1(YDL006W)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:QDR2(YIL121W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RAD14(YMR201C)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD51(YER095W)|FD-Score:5.09|P-value:1.79E-7||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD55(YDR076W)|FD-Score:5.02|P-value:2.55E-7||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RPP1B(YDL130W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS28B(YLR264W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTT101(YJL047C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SEA4(YBL104C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SIF2(YBR103W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SSE1(YPL106C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSU1(YPL092W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein Gene:TAT1(YBR069C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCM62(YBR044C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TCO89(YPL180W)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:THI21(YPL258C)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TIM11(YDR322C-A)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:VAC7(YNL054W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:XRS2(YDR369C)|FD-Score:4.33|P-value:7.50E-6||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBL012C(YBL012C_d)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL071C(YBL071C_d)|FD-Score:5.36|P-value:4.20E-8||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YDL172C(YDL172C_d)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL014C-A(YIL014C-A_p)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function Gene:YIR042C(YIR042C_p)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YKL031W(YKL031W_d)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKR051W(YKR051W_p)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Putative protein of unknown function Gene:YLR428C(YLR428C_d)|FD-Score:4.27|P-value:9.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADE5,7(YGL234W)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AIM25(YJR100C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ASN1(YPR145W)|FD-Score:5.07|P-value:2.04E-7||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BCS1(YDR375C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BDF1(YLR399C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BEM4(YPL161C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BPT1(YLL015W)|FD-Score:3.54|P-value:1.96E-4||SGD DESC:ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p Gene:BRP1(YGL007W_d)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CCM1(YGR150C)|FD-Score:4.65|P-value:1.70E-6||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDC10(YCR002C)|FD-Score:3.82|P-value:6.78E-5||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CLD1(YGR110W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CNM67(YNL225C)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CRD1(YDL142C)|FD-Score:5.24|P-value:7.90E-8||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:CSR2(YPR030W)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:DAL1(YIR027C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DOA4(YDR069C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DSF2(YBR007C_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EMC4(YGL231C)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERS1(YCR075C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:EXO1(YOR033C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FAR11(YNL127W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GIT1(YCR098C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:HUL4(YJR036C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:ICE2(YIL090W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IRC23(YOR044W)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:IRC5(YFR038W)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:KRE6(YPR159W)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:MNN2(YBR015C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:NCR1(YPL006W)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:PTC1(YDL006W)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:QDR2(YIL121W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RAD14(YMR201C)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD51(YER095W)|FD-Score:5.09|P-value:1.79E-7||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD55(YDR076W)|FD-Score:5.02|P-value:2.55E-7||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RPP1B(YDL130W)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RPS28B(YLR264W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTT101(YJL047C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SEA4(YBL104C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SIF2(YBR103W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SSE1(YPL106C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SSU1(YPL092W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein Gene:TAT1(YBR069C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCM62(YBR044C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TCO89(YPL180W)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:THI21(YPL258C)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TIM11(YDR322C-A)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:VAC7(YNL054W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:XRS2(YDR369C)|FD-Score:4.33|P-value:7.50E-6||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBL012C(YBL012C_d)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL071C(YBL071C_d)|FD-Score:5.36|P-value:4.20E-8||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YDL172C(YDL172C_d)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL014C-A(YIL014C-A_p)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function Gene:YIR042C(YIR042C_p)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YKL031W(YKL031W_d)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKR051W(YKR051W_p)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Putative protein of unknown function Gene:YLR428C(YLR428C_d)|FD-Score:4.27|P-value:9.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL143C23.106.02E-11816.80SSL2Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
YER165W6.242.12E-101.06PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YBL097W5.181.08E-70.86BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YJL081C4.327.83E-60.28ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YFR037C4.042.73E-50.03RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YML127W4.013.10E-50.02RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YKR079C3.983.38E-50.23TRZ1tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2
YMR033W3.758.80E-50.07ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YLR106C3.681.15E-40.03MDN1Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
YKL049C3.651.29E-40.15CSE4Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions
YLR249W3.512.28E-40.03YEF3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
YOR174W3.482.55E-40.13MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YPL122C3.344.18E-40.14TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YDR016C3.206.79E-40.02DAD1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YBR029C3.197.16E-40.02CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL071C_d5.364.20E-8YBL071C_dDubious open reading frame, predicted protein contains a peroxisomal targeting signal
YDL142C5.247.90E-8CRD1Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis
YER095W5.091.79E-7RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YPR145W5.072.04E-7ASN1Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
YDR076W5.022.55E-7RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
YNL127W4.721.19E-6FAR11Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2
YPL161C4.691.38E-6BEM4Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
YGR150C4.651.70E-6CCM1Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport
YJR100C4.424.90E-6AIM25Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss
YFR038W4.415.16E-6IRC5Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci
YDR369C4.337.50E-6XRS2Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
YLR428C_d4.279.74E-6YLR428C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1
YKL031W_d4.261.01E-5YKL031W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species
YBR015C4.211.26E-5MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YDL172C_d4.171.54E-5YDL172C_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1085
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5260SGTC_272doxorubicin 8.1 μMMiscellaneous317031anthracycline transcription coupled DNA repair
0.4601.51E-307SGTC_257daunorubicin 18.5 μMMiscellaneous303230.855263anthracycline transcription coupled DNA repair
0.4362.38E-268SGTC_2662daunorubicin 22.4 μMMiscellaneous303230.855263anthracycline transcription coupled DNA repair
0.3582.95E-178SGTC_2747doxorubicin 7.6 μMMiscellaneous317031anthracycline transcription coupled DNA repair
0.2911.85E-115SGTC_2746daunorubicin 9.9 μMMiscellaneous303230.855263anthracycline transcription coupled DNA repair
0.2264.49E-69SGTC_1105epirubicin 18.1 μMNIH Clinical Collection653481anthracycline transcription coupled DNA repair
0.1373.15E-26SGTC_1079idarubicin 2.4 μMNIH Clinical Collection6363620.705128DNA damage response
0.1301.52E-23SGTC_12290139-0193 12.2 μMChemDiv (Drug-like library)30907520.021978RSC complex & mRNA processing
0.1292.37E-23SGTC_8291635-0120 117.0 μMChemDiv (Drug-like library)2850540.0421053DNA damage response
0.1287.75E-23SGTC_23619034121 116.0 μMChembridge (Fragment library)64969170.0612245RSC complex & mRNA processing
0.1201.74E-20SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.0888889RSC complex & mRNA processing
0.1132.64E-18SGTC_22095-(5-methyl-2-thenylidene)-Barbituric acid 122.8 μMChembridge (Fragment library)6167640.0638298
0.1101.97E-17SGTC_10503232-1156 69.3 μMChemDiv (Drug-like library)7005290.09375RSC complex & mRNA processing
0.1095.57E-17SGTC_1707st032323 6.3 μMTimTec (Natural product derivative library)31320570.11215DNA damage response
0.1071.80E-16SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.107843RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_272doxorubicin8.13 μM131703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_2747doxorubicin7.63 μM131703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_1105epirubicin18.1 μM165348NIH Clinical Collection579.98020.338712anthracycline transcription coupled DNA repair
SGTC_257daunorubicin18.5 μM0.85526330323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2662daunorubicin22.37 μM0.85526330323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2746daunorubicin9.88 μM0.85526330323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_1079idarubicin2.36 μM0.705128636362NIH Clinical Collection533.954821.026610DNA damage response
SGTC_2611pyromycin23.93 μM0.398058196990Microsource (Natural product library)585.5991.992512
SGTC_2738aclarubicin5.55 μM0.347107451415Miscellaneous811.867922.682416RNA pol III & RNase P/MRP
SGTC_2410aloe-emodin-8-o-glycoside46.3 μM0.26804114077415TimTec (Pure natural product library)432.3775-0.21610mitochondrial processes
SGTC_1569carminic acid40.6 μM0.25252514749TimTec (Pure natural product library)492.3864-0.436913
SGTC_2490carminic acid95.39 μM0.25252514749TimTec (Pure natural product library)492.3864-0.436913
SGTC_15892',5'-dimethoxyflavone47.2 μM0.218391688667TimTec (Pure natural product library)282.290663.10304
SGTC_11700310-012764.8 μM0.213483774975ChemDiv (Drug-like library)251.236862.98804