toremifene FDA approved compound

2-[4-[(Z)-4-chloro-1,2-diphenylbut-1-enyl]phenoxy]-N,N-dimethylethanamine

A first generation selective estrogen receptor modulator (SERM). Like tamoxifen, it is an estrogen agonist for bone tissue and cholesterol metabolism but is antagonistic on mammary and uterine tissue.

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PubChem MeSH terms: Antineoplastic Agents, Hormonal;Bone Density Conservation Agents;Selective Estrogen Receptor Modulators



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1092
Screen concentration 6.3 μM
Source NIH Clinical Collection
PubChem CID 3005573
SMILES CN(C)CCOC1=CC=C(C=C1)C(=C(CCCl)C2=CC=CC=C2)C3=CC=CC=C3
Standardized SMILES CN(C)CCOc1ccc(cc1)C(=C(CCCl)c2ccccc2)c3ccccc3
Molecular weight 405.9596
ALogP 6.3
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.27
% growth inhibition (Hom. pool) 2.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3005573
Download HIP data (tab-delimited text)  (excel)
Gene:DBP6(YNR038W)|FD-Score:-3.87|P-value:5.35E-5|Clearance:0||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:DED1(YOR204W)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.4||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:LSG1(YGL099W)|FD-Score:5.48|P-value:2.17E-8|Clearance:1.15||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MOT1(YPL082C)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.09||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NEO1(YIL048W)|FD-Score:9.12|P-value:3.73E-20|Clearance:3.33||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NIP1(YMR309C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.04||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NMD3(YHR170W)|FD-Score:4.33|P-value:7.60E-6|Clearance:0.51||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRI2(YKL045W)|FD-Score:3.09|P-value:9.89E-4|Clearance:0.01||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPL32(YBL092W)|FD-Score:-3.12|P-value:9.17E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RSC6(YCR052W)|FD-Score:3.21|P-value:6.60E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEN34(YAR008W)|FD-Score:5.79|P-value:3.50E-9|Clearance:0.31||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:TRS120(YDR407C)|FD-Score:-3.27|P-value:5.30E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YIF1(YNL263C)|FD-Score:-3.44|P-value:2.90E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:YLR230W(YLR230W_d)|FD-Score:3.82|P-value:6.67E-5|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:DBP6(YNR038W)|FD-Score:-3.87|P-value:5.35E-5|Clearance:0||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:DED1(YOR204W)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.4||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:LSG1(YGL099W)|FD-Score:5.48|P-value:2.17E-8|Clearance:1.15||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MOT1(YPL082C)|FD-Score:3.18|P-value:7.31E-4|Clearance:0.09||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NEO1(YIL048W)|FD-Score:9.12|P-value:3.73E-20|Clearance:3.33||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NIP1(YMR309C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.04||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NMD3(YHR170W)|FD-Score:4.33|P-value:7.60E-6|Clearance:0.51||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRI2(YKL045W)|FD-Score:3.09|P-value:9.89E-4|Clearance:0.01||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPL32(YBL092W)|FD-Score:-3.12|P-value:9.17E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RSC6(YCR052W)|FD-Score:3.21|P-value:6.60E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEN34(YAR008W)|FD-Score:5.79|P-value:3.50E-9|Clearance:0.31||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:TRS120(YDR407C)|FD-Score:-3.27|P-value:5.30E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YIF1(YNL263C)|FD-Score:-3.44|P-value:2.90E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:YLR230W(YLR230W_d)|FD-Score:3.82|P-value:6.67E-5|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3005573
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APL3(YBL037W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ARK1(YNL020C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ART10(YLR392C)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:ASC1(YMR116C)|FD-Score:4.8|P-value:7.75E-7||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BUD28(YLR062C_d)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAF20(YOR276W)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CAN1(YEL063C)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CBP1(YJL209W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCS1(YMR038C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CKB2(YOR039W)|FD-Score:5.61|P-value:1.03E-8||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLG1(YGL215W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:CNN1(YFR046C)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:DNF1(YER166W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ERP5(YHR110W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAR10(YLR238W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FPR2(YDR519W)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:FUN14(YAL008W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Mitochondrial protein of unknown function Gene:HAP2(YGL237C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HFA1(YMR207C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HIS2(YFR025C)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HSP82(YPL240C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:ITC1(YGL133W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:LDB16(YCL005W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LOA1(YPR139C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MAG2(YLR427W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MAK3(YPR051W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MGA1(YGR249W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MTC3(YGL226W)|FD-Score:3.09|P-value:0.00100||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MTO1(YGL236C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:OCA1(YNL099C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OST6(YML019W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PBS2(YJL128C)|FD-Score:7.16|P-value:4.05E-13||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PDA1(YER178W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:POL4(YCR014C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:POR1(YNL055C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:5.15|P-value:1.32E-7||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PXA2(YKL188C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:QRI5(YLR204W)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RGL1(YPL066W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL11B(YGR085C)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:SFM1(YOR021C)|FD-Score:-3.79|P-value:7.45E-5||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SKY1(YMR216C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SMI1(YGR229C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPO14(YKR031C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SSK22(YCR073C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:STV1(YMR054W)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SYH1(YPL105C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX5(YDR330W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:VFA1(YER128W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMA7(YGR020C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YBR174C(YBR174C_d)|FD-Score:5.03|P-value:2.42E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YGR242W(YGR242W_d)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR138C(YHR138C_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YLR171W(YLR171W_d)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR264C-A(YLR264C-A_p)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Putative protein of unknown function Gene:YMR317W(YMR317W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YOL098C(YOL098C_p)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Putative metalloprotease Gene:YOL114C(YOL114C_p)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOR072W(YOR072W_d)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR170W(YOR170W_d)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-5.34|P-value:4.76E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR170C(YPR170C_d)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:AFT1(YGL071W)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APL3(YBL037W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport Gene:ARK1(YNL020C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ART10(YLR392C)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene Gene:ASC1(YMR116C)|FD-Score:4.8|P-value:7.75E-7||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BUD28(YLR062C_d)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAF20(YOR276W)|FD-Score:-3.11|P-value:9.47E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:CAN1(YEL063C)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CBP1(YJL209W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCS1(YMR038C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CKB2(YOR039W)|FD-Score:5.61|P-value:1.03E-8||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLG1(YGL215W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:CNN1(YFR046C)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:DNF1(YER166W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ERP5(YHR110W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAR10(YLR238W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FPR2(YDR519W)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:FUN14(YAL008W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Mitochondrial protein of unknown function Gene:HAP2(YGL237C)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HFA1(YMR207C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HIS2(YFR025C)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HSP82(YPL240C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:ITC1(YGL133W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:LDB16(YCL005W)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LOA1(YPR139C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA Gene:MAG2(YLR427W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MAK3(YPR051W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MGA1(YGR249W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:MTC3(YGL226W)|FD-Score:3.09|P-value:0.00100||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MTO1(YGL236C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:OCA1(YNL099C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OST6(YML019W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PBS2(YJL128C)|FD-Score:7.16|P-value:4.05E-13||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PDA1(YER178W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:POL4(YCR014C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:POR1(YNL055C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:5.15|P-value:1.32E-7||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PXA2(YKL188C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:QRI5(YLR204W)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RGL1(YPL066W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL11B(YGR085C)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:SFM1(YOR021C)|FD-Score:-3.79|P-value:7.45E-5||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SKY1(YMR216C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SMI1(YGR229C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SPO14(YKR031C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SSK22(YCR073C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:STV1(YMR054W)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SYH1(YPL105C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX5(YDR330W)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p Gene:VFA1(YER128W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMA7(YGR020C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YBR174C(YBR174C_d)|FD-Score:5.03|P-value:2.42E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YGR242W(YGR242W_d)|FD-Score:3.79|P-value:7.46E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR138C(YHR138C_p)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YLR171W(YLR171W_d)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR264C-A(YLR264C-A_p)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Putative protein of unknown function Gene:YMR317W(YMR317W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function with some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene Gene:YOL098C(YOL098C_p)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Putative metalloprotease Gene:YOL114C(YOL114C_p)|FD-Score:4.43|P-value:4.66E-6||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOR072W(YOR072W_d)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR170W(YOR170W_d)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-5.34|P-value:4.76E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR170C(YPR170C_d)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W9.123.73E-203.33NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YAR008W5.793.50E-90.32SEN34Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
YGL099W5.482.17E-81.15LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W4.337.60E-60.51NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YLR230W_d3.826.67E-50.17YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YOR204W3.651.30E-40.40DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YMR309C3.255.68E-40.04NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YCR052W3.216.60E-40.03RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YPL082C3.187.31E-40.09MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YKL045W3.099.89E-40.01PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YNL189W3.080.001040.02SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YLR026C3.060.001100.00SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YLR310C3.060.001110.00CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YMR227C3.060.001120.01TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YGL018C3.050.001150.29JAC1Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL128C7.164.05E-13PBS2MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
YOR039W5.611.03E-8CKB2Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
YDL006W5.151.32E-7PTC1Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation
YGL071W5.131.45E-7AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YLR392C5.071.99E-7ART10Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene
YBR174C_d5.032.42E-7YBR174C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective
YDR519W4.934.02E-7FPR2Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress
YMR116C4.807.75E-7ASC1G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation
YGL236C4.731.12E-6MTO1Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants
YEL063C4.651.64E-6CAN1Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
YOR072W_d4.651.66E-6YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YPL066W4.651.69E-6RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YKR031C4.612.00E-6SPO14Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
YOL114C_p4.434.66E-6YOL114C_pPutative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene
YMR054W4.405.50E-6STV1Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole

GO enrichment analysis for SGTC_1092
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2734.37E-101SGTC_270tamoxifen 5.5 μMMiscellaneous53760.738095NEO1
0.2314.83E-72SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.0714286NEO1
0.2198.25E-65SGTC_1665st014639 11.4 μMTimTec (Natural product derivative library)9811330.127907NEO1
0.2125.28E-61SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.0714286NEO1
0.2081.01E-58SGTC_1680diphenhydramine 100.0 μMNIH Clinical Collection89800.340426NEO1
0.2031.04E-55SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.235294NEO1
0.2011.02E-54SGTC_9403232-1287 54.8 μMChemDiv (Drug-like library)15854930.222222NEO1
0.1956.69E-52SGTC_2697mebhydrolin 72.4 μMTimTec (Natural product derivative library)225300.119403NEO1
0.1931.67E-50SGTC_2742clothiapine 36.3 μMMiscellaneous163510.0972222NEO1
0.1904.16E-49SGTC_9001112-0045 141.0 μMChemDiv (Drug-like library)562110.0923077NEO1
0.1891.36E-48SGTC_11303454-2858 15.9 μMChemDiv (Drug-like library)7156280.101449NEO1
0.1891.84E-48SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.103448NEO1
0.1879.98E-48SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.206349NEO1
0.1871.11E-47SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0847458NEO1
0.1843.12E-46SGTC_1797st048695 56.8 μMTimTec (Natural product derivative library)5922620.123288NEO1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2769toremifene3.73 μM13005573Miscellaneous405.959626.29802
SGTC_270tamoxifen5.46 μM0.7380955376Miscellaneous371.514566.31902NEO1
SGTC_2774tamoxifen10.92 μM0.7380952733526Miscellaneous371.514566.31902
SGTC_2757clomiphene4.22 μM0.5102041548953Miscellaneous405.959626.48302fatty acid desaturase (OLE1)
SGTC_1680diphenhydramine100 μM0.3404268980NIH Clinical Collection291.815683.76612NEO1
SGTC_3260913710949.47 μM0.33962317478346Chembridge (Drug-like library)255.311683.1561260S ribosome export
SGTC_2929901334824.6 μM0.2982462995952Chembridge (Drug-like library)310.175223.99912
SGTC_12420402-010360.1 μM0.285714432868ChemDiv (Drug-like library)191.269482.3102
SGTC_2418pentamidine31.67 μM0.2857144735Miscellaneous340.419462.65744mitochondrial processes
SGTC_2640pentamidine10.38 μM0.2857144735Miscellaneous340.419462.65744mitochondrial processes
SGTC_3310913022944.14 μM0.2833337761258Chembridge (Drug-like library)289.756743.8212Golgi
SGTC_1290088-0017176.64 μM0.280702182446ChemDiv (Drug-like library)278.343483.63914