0850-0284

1-(3-bromophenyl)-N-[2-(3-methylphenyl)-1,3-benzoxazol-5-yl]methanimine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_110
Screen concentration 111.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 3906265
SMILES CC1=CC=CC(=C1)C2=NC3=C(O2)C=CC(=C3)N=CC4=CC(=CC=C4)Br
Standardized SMILES Cc1cccc(c1)c2oc3ccc(cc3n2)N=Cc4cccc(Br)c4
Molecular weight 391.2606
ALogP 6.11
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 25.11
% growth inhibition (Hom. pool) 11.73


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3906265
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:-3.12|P-value:8.99E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CAB4(YGR277C)|FD-Score:-3.74|P-value:9.16E-5|Clearance:0||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:DBF4(YDR052C)|FD-Score:-3.74|P-value:9.06E-5|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DED1(YOR204W)|FD-Score:-3.19|P-value:7.16E-4|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:NMD3(YHR170W)|FD-Score:-4.61|P-value:1.98E-6|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE1(YLR007W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.07||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RLP24(YLR009W)|FD-Score:-3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:4.69|P-value:1.36E-6|Clearance:0.74||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.95|P-value:3.89E-5|Clearance:0.25||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RSE1(YML049C)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEC21(YNL287W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.1||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:TFB1(YDR311W)|FD-Score:-3.75|P-value:8.96E-5|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TFB2(YPL122C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.63||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:YKL153W(YKL153W_d)|FD-Score:-3.16|P-value:7.96E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YPT1(YFL038C)|FD-Score:6.45|P-value:5.62E-11|Clearance:1.76||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:ASA1(YPR085C)|FD-Score:-3.12|P-value:8.99E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CAB4(YGR277C)|FD-Score:-3.74|P-value:9.16E-5|Clearance:0||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:DBF4(YDR052C)|FD-Score:-3.74|P-value:9.06E-5|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DED1(YOR204W)|FD-Score:-3.19|P-value:7.16E-4|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:NMD3(YHR170W)|FD-Score:-4.61|P-value:1.98E-6|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE1(YLR007W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.07||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RLP24(YLR009W)|FD-Score:-3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:4.69|P-value:1.36E-6|Clearance:0.74||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:3.95|P-value:3.89E-5|Clearance:0.25||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RSE1(YML049C)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEC21(YNL287W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.1||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:TFB1(YDR311W)|FD-Score:-3.75|P-value:8.96E-5|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators Gene:TFB2(YPL122C)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.63||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:YKL153W(YKL153W_d)|FD-Score:-3.16|P-value:7.96E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YPT1(YFL038C)|FD-Score:6.45|P-value:5.62E-11|Clearance:1.76||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3906265
Download HOP data (tab-delimited text)  (excel)
Gene:ADH2(YMR303C)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:ADH5(YBR145W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:5.96|P-value:1.29E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATP22(YDR350C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BTS1(YPL069C)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:DAL81(YIR023W)|FD-Score:6.32|P-value:1.29E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSE4(YNR067C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:ERG3(YLR056W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:GPT2(YKR067W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GUP1(YGL084C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GYP1(YOR070C)|FD-Score:5.66|P-value:7.44E-9||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HTA1(YDR225W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IRC4(YDR540C)|FD-Score:-4.9|P-value:4.72E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:5.27|P-value:6.87E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LTV1(YKL143W)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MTC4(YBR255W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:OPY2(YPR075C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PTC1(YDL006W)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RAD17(YOR368W)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RPL20B(YOR312C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL21A(YBR191W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RVS161(YCR009C)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SDC25(YLL016W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SHP1(YBL058W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SLT2(YHR030C)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF4(YGL115W)|FD-Score:5.61|P-value:9.84E-9||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SSO2(YMR183C)|FD-Score:7.13|P-value:5.08E-13||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:SYS1(YJL004C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TPN1(YGL186C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:5.35|P-value:4.43E-8||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.68|P-value:6.59E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.93|P-value:2.12E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VMA5(YKL080W)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YDL109C(YDL109C_p)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:7.68|P-value:7.98E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER135C(YER135C_d)|FD-Score:-4.67|P-value:1.51E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YNL200C(YNL200C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR063C(YPR063C_p)|FD-Score:3.95|P-value:3.99E-5||SGD DESC:ER-localized protein of unknown function Gene:ADH2(YMR303C)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:ADH5(YBR145W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:5.96|P-value:1.29E-9||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATP22(YDR350C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Specific translational activator for the mitochondrial ATP6 mRNA, encoding a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane Gene:BTS1(YPL069C)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:DAL81(YIR023W)|FD-Score:6.32|P-value:1.29E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSE4(YNR067C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:ERG3(YLR056W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:GPT2(YKR067W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GUP1(YGL084C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:GYP1(YOR070C)|FD-Score:5.66|P-value:7.44E-9||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HTA1(YDR225W)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IRC4(YDR540C)|FD-Score:-4.9|P-value:4.72E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:5.27|P-value:6.87E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LTV1(YKL143W)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MTC4(YBR255W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:OPY2(YPR075C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PTC1(YDL006W)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RAD17(YOR368W)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RPL20B(YOR312C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL21A(YBR191W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:-3.1|P-value:9.80E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RVS161(YCR009C)|FD-Score:3.96|P-value:3.80E-5||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SDC25(YLL016W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SHP1(YBL058W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SLT2(YHR030C)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF4(YGL115W)|FD-Score:5.61|P-value:9.84E-9||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SSO2(YMR183C)|FD-Score:7.13|P-value:5.08E-13||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:SYS1(YJL004C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TPN1(YGL186C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:5.35|P-value:4.43E-8||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.68|P-value:6.59E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.93|P-value:2.12E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS85(YDR108W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VMA5(YKL080W)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YDL109C(YDL109C_p)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:7.68|P-value:7.98E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER119C-A(YER119C-A_d)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER135C(YER135C_d)|FD-Score:-4.67|P-value:1.51E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YNL200C(YNL200C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR063C(YPR063C_p)|FD-Score:3.95|P-value:3.99E-5||SGD DESC:ER-localized protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFL038C6.455.62E-111.76YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YHR062C4.691.36E-60.74RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR167W3.953.89E-50.25RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YNL287W3.701.06E-40.10SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YLR007W3.601.59E-40.07NSE1Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YPL122C3.532.09E-40.63TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YML031W2.900.001890.08NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YBR154C2.810.002450.10RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YLR033W2.720.003310.04RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YAL034W-A2.680.003680.18MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YDR228C2.500.006220.02PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YDL145C2.480.006510.12COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YOR372C2.370.008970.01NDD1Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
YHR069C2.360.009190.05RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YLR208W2.300.010600.04SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR008C_d7.687.98E-15YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR183C7.135.08E-13SSO2Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p
YKL211C6.932.12E-12TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YIR023W6.321.29E-10DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR127W5.961.29E-9ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YER090W5.686.59E-9TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YOR070C5.667.44E-9GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YGL115W5.619.84E-9SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YDR007W5.354.43E-8TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YKR019C5.276.87E-8IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YHR030C4.582.37E-6SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YJL004C4.161.58E-5SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YGL084C4.102.03E-5GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YGL026C4.032.76E-5TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YCR009C3.963.80E-5RVS161Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress

GO enrichment analysis for SGTC_110
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4292.11E-262SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0309278
0.3896.04E-213SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.108108
0.3663.71E-187SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0641026
0.3575.60E-177SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.102941TRP & mitochondrial translation
0.3531.08E-172SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.128571
0.3502.24E-169SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.04
0.3454.42E-165SGTC_18825615643 20.0 μMMiscellaneous22530750.0898876TRP & mitochondrial translation
0.3441.90E-163SGTC_1090132-0036 33.7 μMChemDiv (Drug-like library)672740.0779221
0.3403.57E-159SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0666667
0.3376.55E-157SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.121622
0.3321.98E-151SGTC_18815652484 16.0 μMMiscellaneous22551290.246753TRP & mitochondrial translation
0.3245.17E-144SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.084507
0.3191.04E-139SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA
0.3176.72E-138SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.128205
0.3163.07E-137SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0365854

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1380850-025940.92 μM0.6896556813004ChemDiv (Drug-like library)407.265.04614cell wall
SGTC_7771315-0167517 μM0.6379314221767ChemDiv (Drug-like library)381.254666.69203Golgi
SGTC_7381315-000372.4 μM0.5555566251474ChemDiv (Drug-like library)372.846886.38803
SGTC_7981287-0182476 μM0.551649755ChemDiv (Drug-like library)420.301825.78704
SGTC_5741315-039740 μM0.5373136794277ChemDiv (Drug-like library)437.285984.17615
SGTC_8071315-015148 μM0.5151524011080ChemDiv (Drug-like library)393.39425.6805
SGTC_6051315-0399119 μM0.49206324020461ChemDiv (Drug-like library)381.254666.69203Golgi
SGTC_12131315-0166115 μM0.466667768401ChemDiv (Drug-like library)266.294562.88713
SGTC_9711315-038426.7 μM0.4520556769120ChemDiv (Drug-like library)451.312564.52515cell wall signaling
SGTC_14524194-0072190 μM0.4142861343545ChemDiv (Drug-like library)408.248064.14914