amiodarone FDA approved compound

(2-butyl-1-benzofuran-3-yl)-[4-[2-(diethylamino)ethoxy]-3,5-diiodophenyl]methanone hydrochloride

Amiodarone is a non-selective ion channel blocker with broad fungicidal activity. Amiodarone induces an immediate influx of Ca2+ inSaccharomyces cerevisiae, followed by mitochondrial fragmentation and cell death.

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PubChem MeSH terms: Anti-Arrhythmia Agents;Enzyme Inhibitors;Vasodilator Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1100
Screen concentration 5.1 μM
Source NIH Clinical Collection
PubChem CID 441325
SMILES CCCCC1=C(C2=CC=CC=C2O1)C(=O)C3=CC(=C(C(=C3)I)OCCN(CC)CC)I.Cl
Standardized SMILES CCCCc1oc2ccccc2c1C(=O)c3cc(I)c(OCCN(CC)CC)c(I)c3
Molecular weight 681.7725
ALogP 7.62
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.6
% growth inhibition (Hom. pool) 5.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 441325
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.23||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ATM1(YMR301C)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.23||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CFT1(YDR301W)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.23||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:ESP1(YGR098C)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:LSG1(YGL099W)|FD-Score:3.11|P-value:9.40E-4|Clearance:0.08||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NEO1(YIL048W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.23||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NMD3(YHR170W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.23||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PFY1(YOR122C)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.06||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:PRE1(YER012W)|FD-Score:-3.29|P-value:4.94E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RPF1(YHR088W)|FD-Score:-3.9|P-value:4.86E-5|Clearance:0||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPS20(YHL015W)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.46|P-value:2.65E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:TAF1(YGR274C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.23||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:UTP6(YDR449C)|FD-Score:-4.16|P-value:1.59E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPR136C(YPR136C_d)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ALG14(YBR070C)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.23||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ATM1(YMR301C)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.23||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CFT1(YDR301W)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.23||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:ESP1(YGR098C)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:LSG1(YGL099W)|FD-Score:3.11|P-value:9.40E-4|Clearance:0.08||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NEO1(YIL048W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.23||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NMD3(YHR170W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.23||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PFY1(YOR122C)|FD-Score:3.16|P-value:7.78E-4|Clearance:0.06||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:PRE1(YER012W)|FD-Score:-3.29|P-value:4.94E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RPF1(YHR088W)|FD-Score:-3.9|P-value:4.86E-5|Clearance:0||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RPS20(YHL015W)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.46|P-value:2.65E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:TAF1(YGR274C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.23||SGD DESC:TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression Gene:UTP6(YDR449C)|FD-Score:-4.16|P-value:1.59E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPR136C(YPR136C_d)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 441325
Download HOP data (tab-delimited text)  (excel)
Gene:ACH1(YBL015W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADA2(YDR448W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AIM44(YPL158C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALG5(YPL227C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ATP10(YLR393W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BDH1(YAL060W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:CAC2(YML102W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CKB2(YOR039W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:COS12(YGL263W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CTR1(YPR124W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL81(YIR023W)|FD-Score:6.19|P-value:3.00E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:FYV12(YOR183W_p)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:4.28|P-value:9.31E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GTB1(YDR221W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HMT1(YBR034C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HST1(YOL068C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUT1(YPL244C)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:ICY2(YPL250C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IMP2'(YIL154C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISM1(YPL040C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LCL2(YLR104W_p)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:LDB17(YDL146W)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LEA1(YPL213W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:MAL32(YBR299W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MAP1(YLR244C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MHO1(YJR008W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MIH1(YMR036C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MNN11(YJL183W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MON1(YGL124C)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MUS81(YDR386W)|FD-Score:3.76|P-value:8.63E-5||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:PKH3(YDR466W)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:POR1(YNL055C)|FD-Score:6.46|P-value:5.26E-11||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:RHB1(YCR027C)|FD-Score:-5.85|P-value:2.41E-9||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPS8A(YBL072C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SAP185(YJL098W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication Gene:SHR5(YOL110W)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SKI2(YLR398C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SUM1(YDR310C)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUR4(YLR372W)|FD-Score:5.48|P-value:2.10E-8||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWR1(YDR334W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TIF4632(YGL049C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP2(YER090W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VMA16(YHR026W)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS35(YJL154C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS38(YLR360W)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:WSS1(YHR134W)|FD-Score:3.79|P-value:7.66E-5||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YDR338C(YDR338C_p)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YDR521W(YDR521W_d)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YET3(YDL072C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YFR006W(YFR006W_p)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL072C(YGL072C_d)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL199C(YGL199C_d)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YLR257W(YLR257W_p)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YMR087W(YMR087W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL194C(YNL194C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YNR042W(YNR042W_d)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Gene:YOR082C(YOR082C_d)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YOR223W(YOR223W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:ACH1(YBL015W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADA2(YDR448W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AIM44(YPL158C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALG5(YPL227C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ATP10(YLR393W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BDH1(YAL060W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:CAC2(YML102W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CKB2(YOR039W)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:COS12(YGL263W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CTR1(YPR124W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL81(YIR023W)|FD-Score:6.19|P-value:3.00E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:FYV12(YOR183W_p)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL11(YOL051W)|FD-Score:4.28|P-value:9.31E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GTB1(YDR221W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HMT1(YBR034C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HST1(YOL068C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUT1(YPL244C)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1 Gene:ICY2(YPL250C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:IMP2'(YIL154C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISM1(YPL040C)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LCL2(YLR104W_p)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:LDB17(YDL146W)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LEA1(YPL213W)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:MAL32(YBR299W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MAP1(YLR244C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MHO1(YJR008W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MIH1(YMR036C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25 Gene:MNN11(YJL183W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MON1(YGL124C)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MUS81(YDR386W)|FD-Score:3.76|P-value:8.63E-5||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:PKH3(YDR466W)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:POR1(YNL055C)|FD-Score:6.46|P-value:5.26E-11||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:RHB1(YCR027C)|FD-Score:-5.85|P-value:2.41E-9||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPS8A(YBL072C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SAP185(YJL098W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication Gene:SHR5(YOL110W)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SKI2(YLR398C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SUM1(YDR310C)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUR4(YLR372W)|FD-Score:5.48|P-value:2.10E-8||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWR1(YDR334W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TIF4632(YGL049C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP2(YER090W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VMA16(YHR026W)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS35(YJL154C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS38(YLR360W)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:WSS1(YHR134W)|FD-Score:3.79|P-value:7.66E-5||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YDR338C(YDR338C_p)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YDR521W(YDR521W_d)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YET3(YDL072C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YFR006W(YFR006W_p)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL072C(YGL072C_d)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL199C(YGL199C_d)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YLR257W(YLR257W_p)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YMR087W(YMR087W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL194C(YNL194C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YNR042W(YNR042W_d)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2 Gene:YOR082C(YOR082C_d)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YOR223W(YOR223W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W3.711.03E-40.23NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YHR170W3.671.21E-40.23NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDR301W3.601.62E-40.23CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YBR070C3.432.96E-40.23ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YMR301C3.433.02E-40.23ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YGR274C3.393.45E-40.23TAF1TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression
YOR122C3.167.78E-40.06PFY1Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress
YGL099W3.119.40E-40.08LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YGR120C3.020.001240.07COG2Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YMR197C2.960.001540.04VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YNL267W2.920.001740.03PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YMR117C2.890.001920.02SPC24Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YOR074C2.870.002070.12CDC21Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
YML126C2.750.003009.37E-4ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
YKL004W2.750.003010.03AUR1Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL055C6.465.26E-11POR1Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication
YIR023W6.193.00E-10DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YLR372W5.482.10E-8SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YML102W5.151.31E-7CAC2Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription
YPL158C4.711.24E-6AIM44Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress
YIL154C4.691.38E-6IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YDR310C4.552.72E-6SUM1Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
YPL213W4.503.44E-6LEA1Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
YOR082C_d4.454.38E-6YOR082C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W
YOL051W4.289.31E-6GAL11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
YLR244C4.211.27E-5MAP1Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p
YMR087W4.211.27E-5YMR087WPutative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain
YBR034C4.111.95E-5HMT1Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region.
YOR039W4.022.96E-5CKB2Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
YOR223W4.013.03E-5YOR223WSubunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3

GO enrichment analysis for SGTC_1100
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1783.33E-43SGTC_486niguldipine 82.0 μMMiscellaneous12360.132075amide catabolism
0.1461.37E-29SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.0941176
0.1454.21E-29SGTC_11303454-2858 15.9 μMChemDiv (Drug-like library)7156280.0952381NEO1
0.1447.94E-29SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.0823529amide catabolism
0.1441.01E-28SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.115385
0.1381.64E-26SGTC_270tamoxifen 5.5 μMMiscellaneous53760.219178NEO1
0.1353.16E-25SGTC_20365160441 105.0 μMChembridge (Fragment library)1345340.108108NEO1
0.1339.64E-25SGTC_24605552655 174.2 μMMiscellaneous54019890.097561amide catabolism
0.1288.02E-23SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.135802NEO1
0.1271.68E-22SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.123596NEO1
0.1263.67E-22SGTC_1797st048695 56.8 μMTimTec (Natural product derivative library)5922620.126437NEO1
0.1254.68E-22SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.125
0.1217.54E-21SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.0705882NEO1
0.1202.49E-20SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.0886076
0.1194.08E-20SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.164557

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_439amiodarone34.4 μM12157Miscellaneous645.31167.23804NEO1-PIK1
SGTC_10953-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran15.5 μM0.4242422333NIH Clinical Collection424.083385.60413
SGTC_2757clomiphene4.22 μM0.3043481548953Miscellaneous405.959626.48302fatty acid desaturase (OLE1)
SGTC_150266-008627.1 μM0.28751712902ChemDiv (Drug-like library)378.50725.23603endomembrane recycling
SGTC_492tmb-8126 μM0.2777785494ICCB bioactive library395.532884.93906
SGTC_3347918872144.72 μM0.2544118411Chembridge (Drug-like library)263.719462.86923
SGTC_1614st00238348.6 μM0.2470591947235TimTec (Natural product derivative library)379.495264.51223cell wall signaling
SGTC_269exalamide29 μM0.2463773316Miscellaneous221.295463.05612ergosterol biosynthesis
SGTC_2248tofa1.1 μM0.242857115175Miscellaneous324.454986.85314sphingolipid biosynthesis & PDR1
SGTC_2416tofa880 nM0.242857115175Miscellaneous324.454986.85314sphingolipid biosynthesis & PDR1
SGTC_218alverine citrate93.75 μM0.24193521718Miscellaneous473.55861.03748fatty acid desaturase (OLE1)