duloxetine

(3S)-N-methyl-3-naphthalen-1-yloxy-3-thiophen-2-ylpropan-1-amine

A selective serotonin reuptake inhibitor.

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PubChem MeSH terms: Adrenergic Uptake Inhibitors;Antidepressive Agents;Dopamine Uptake Inhibitors;Serotonin Uptake Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1103
Screen concentration 36.1 μM
Source NIH Clinical Collection
PubChem CID 60835
SMILES CNCCC(C1=CC=CS1)OC2=CC=CC3=CC=CC=C32
Standardized SMILES CNCCC(Oc1cccc2ccccc12)c3cccs3
Molecular weight 297.4146
ALogP 3.85
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.65
% growth inhibition (Hom. pool) 4.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 60835
Download HIP data (tab-delimited text)  (excel)
Gene:ABD1(YBR236C)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.12||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:BRR6(YGL247W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.02||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CCT2(YIL142W)|FD-Score:5.11|P-value:1.62E-7|Clearance:0.68||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CSE1(YGL238W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.07||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:HSH49(YOR319W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.13||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HYP2(YEL034W)|FD-Score:9.85|P-value:3.44E-23|Clearance:4.74||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.19||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NMD3(YHR170W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.11||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP56(YLR197W)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.09||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RSC6(YCR052W)|FD-Score:4.43|P-value:4.77E-6|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC23(YPR181C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.07||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SLD5(YDR489W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.02||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SUI2(YJR007W)|FD-Score:3.68|P-value:1.19E-4|Clearance:0.04||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:UTP8(YGR128C)|FD-Score:4.37|P-value:6.27E-6|Clearance:0.18||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:4|P-value:3.20E-5|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ABD1(YBR236C)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.12||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:BRR6(YGL247W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.02||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CCT2(YIL142W)|FD-Score:5.11|P-value:1.62E-7|Clearance:0.68||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CSE1(YGL238W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.07||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:HSH49(YOR319W)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.13||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:HYP2(YEL034W)|FD-Score:9.85|P-value:3.44E-23|Clearance:4.74||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.19||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NMD3(YHR170W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.11||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP56(YLR197W)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.09||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RSC6(YCR052W)|FD-Score:4.43|P-value:4.77E-6|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC23(YPR181C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.07||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SLD5(YDR489W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.02||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SUI2(YJR007W)|FD-Score:3.68|P-value:1.19E-4|Clearance:0.04||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:UTP8(YGR128C)|FD-Score:4.37|P-value:6.27E-6|Clearance:0.18||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:4|P-value:3.20E-5|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 60835
Download HOP data (tab-delimited text)  (excel)
Gene:ADH3(YMR083W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:AFT1(YGL071W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AKR1(YDR264C)|FD-Score:4.44|P-value:4.56E-6||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ASG1(YIL130W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATG17(YLR423C)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BEM3(YPL115C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BNR1(YIL159W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BUD16(YEL029C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD27(YFL023W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CIT3(YPR001W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CKB2(YOR039W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLN3(YAL040C)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CMS1(YLR003C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DIA2(YOR080W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:ECM25(YJL201W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:ERV14(YGL054C)|FD-Score:6.49|P-value:4.39E-11||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FLR1(YBR008C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FMP16(YDR070C_p)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GDE1(YPL110C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GLR1(YPL091W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GPR1(YDL035C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HCM1(YCR065W)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HEK2(YBL032W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HHT2(YNL031C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HNT3(YOR258W)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HXT14(YNL318C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IBA57(YJR122W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IXR1(YKL032C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:JNM1(YMR294W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LSC1(YOR142W)|FD-Score:5.74|P-value:4.77E-9||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MAE1(YKL029C)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MCT1(YOR221C)|FD-Score:4.47|P-value:3.96E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM31(YHR194W)|FD-Score:4.78|P-value:8.91E-7||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MIG2(YGL209W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MLH3(YPL164C)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MRPS35(YGR165W)|FD-Score:4.3|P-value:8.57E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MTQ2(YDR140W)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NTH2(YBR001C)|FD-Score:4.38|P-value:6.00E-6||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:RAD61(YDR014W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RDS1(YCR106W)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REI1(YBR267W)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RFM1(YOR279C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGP1(YDR137W)|FD-Score:-3.98|P-value:3.50E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGT1(YKL038W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RIM13(YMR154C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:ROM2(YLR371W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:ROY1(YMR258C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RXT2(YBR095C)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC1(YKL212W)|FD-Score:5|P-value:2.90E-7||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SFP1(YLR403W)|FD-Score:5.28|P-value:6.47E-8||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLH1(YGR271W)|FD-Score:5.06|P-value:2.05E-7||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLT2(YHR030C)|FD-Score:5.75|P-value:4.40E-9||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOP4(YJL192C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO13(YHR014W)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II Gene:SSP2(YOR242C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:TIF1(YKR059W)|FD-Score:5.25|P-value:7.45E-8||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TIP41(YPR040W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TPM1(YNL079C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-6.34|P-value:1.17E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.73|P-value:9.45E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-4.04|P-value:2.67E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP14(YBR058C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:VAM6(YDL077C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA13(YPR036W)|FD-Score:-3.91|P-value:4.69E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS1(YKR001C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:4.53|P-value:2.96E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YCR075W-A(YCR075W-A_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDR455C(YDR455C_d)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YHR210C(YHR210C_p)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YLR413W(YLR413W_p)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YME2(YMR302C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML009W-B(YML009W-B_d)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR320W(YMR320W_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL217W(YNL217W_p)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YPL205C(YPL205C_d)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPL272C(YPL272C_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene Gene:ADH3(YMR083W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:AFT1(YGL071W)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AKR1(YDR264C)|FD-Score:4.44|P-value:4.56E-6||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ASG1(YIL130W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATG17(YLR423C)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BEM3(YPL115C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BNR1(YIL159W)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BUD16(YEL029C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD27(YFL023W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CIT3(YPR001W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CKB2(YOR039W)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLN3(YAL040C)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:CMS1(YLR003C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DIA2(YOR080W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:ECM25(YJL201W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:ERV14(YGL054C)|FD-Score:6.49|P-value:4.39E-11||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FLR1(YBR008C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FMP16(YDR070C_p)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GDE1(YPL110C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GLR1(YPL091W)|FD-Score:4.09|P-value:2.15E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GPR1(YDL035C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HCM1(YCR065W)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HEK2(YBL032W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HHT2(YNL031C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HNT3(YOR258W)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HXT14(YNL318C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IBA57(YJR122W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IXR1(YKL032C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:JNM1(YMR294W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LSC1(YOR142W)|FD-Score:5.74|P-value:4.77E-9||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MAE1(YKL029C)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MCT1(YOR221C)|FD-Score:4.47|P-value:3.96E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM31(YHR194W)|FD-Score:4.78|P-value:8.91E-7||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MIG2(YGL209W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MLH3(YPL164C)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MRPS35(YGR165W)|FD-Score:4.3|P-value:8.57E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MTQ2(YDR140W)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NTH2(YBR001C)|FD-Score:4.38|P-value:6.00E-6||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:RAD61(YDR014W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RDS1(YCR106W)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REI1(YBR267W)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RFM1(YOR279C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGP1(YDR137W)|FD-Score:-3.98|P-value:3.50E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGT1(YKL038W)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RIM13(YMR154C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:ROM2(YLR371W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:ROY1(YMR258C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RXT2(YBR095C)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC1(YKL212W)|FD-Score:5|P-value:2.90E-7||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SFP1(YLR403W)|FD-Score:5.28|P-value:6.47E-8||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLH1(YGR271W)|FD-Score:5.06|P-value:2.05E-7||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLT2(YHR030C)|FD-Score:5.75|P-value:4.40E-9||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOP4(YJL192C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO13(YHR014W)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II Gene:SSP2(YOR242C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:TIF1(YKR059W)|FD-Score:5.25|P-value:7.45E-8||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TIP41(YPR040W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TPM1(YNL079C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:-6.34|P-value:1.17E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.73|P-value:9.45E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-4.04|P-value:2.67E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP14(YBR058C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:VAM6(YDL077C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA13(YPR036W)|FD-Score:-3.91|P-value:4.69E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS1(YKR001C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:4.53|P-value:2.96E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YCR075W-A(YCR075W-A_p)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDR455C(YDR455C_d)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YHR210C(YHR210C_p)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YLR413W(YLR413W_p)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YME2(YMR302C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML009W-B(YML009W-B_d)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR320W(YMR320W_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL217W(YNL217W_p)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YPL205C(YPL205C_d)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPL272C(YPL272C_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YEL034W9.853.44E-234.74HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YIL142W5.111.62E-70.68CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YCR052W4.434.77E-60.06RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YGR128C4.376.27E-60.18UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YER112W4.191.39E-50.19LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YDL196W_d4.003.20E-50.28YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YGL247W3.711.02E-40.02BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YDR489W3.701.09E-40.02SLD5Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YJR007W3.681.19E-40.04SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YGL238W3.641.37E-40.07CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YOR319W3.571.81E-40.13HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YLR197W3.432.97E-40.09NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YHR170W3.344.17E-40.11NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YPR181C3.236.20E-40.07SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YBR236C3.167.86E-40.12ABD1Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL054C6.494.39E-11ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YHR030C5.754.40E-9SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YOR142W5.744.77E-9LSC1Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated
YLR403W5.286.47E-8SFP1Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
YKR059W5.257.45E-8TIF1Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication
YCR106W5.181.13E-7RDS1Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
YDR070C_p5.161.24E-7FMP16_pProtein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
YDR140W5.131.47E-7MTQ2S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
YGR271W5.062.05E-7SLH1Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses
YKL212W5.002.90E-7SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YHR194W4.788.91E-7MDM31Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34
YOR258W4.701.30E-6HNT3DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
YDR129C4.592.23E-6SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YAL040C4.562.59E-6CLN3G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis
YGL095C4.532.96E-6VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment

GO enrichment analysis for SGTC_1103
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1424.99E-28SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.097561heme biosynthesis & mitochondrial translocase
0.1346.91E-25SGTC_32189131285 49.5 μMChembridge (Drug-like library)171564170.1endomembrane recycling
0.1211.23E-20SGTC_22577960413 200.0 μMChembridge (Fragment library)29746890.196429NEO1-PIK1
0.1202.84E-20SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.123288endomembrane recycling
0.1195.29E-20SGTC_2626ethyl orsellinate 100.0 μMMicrosource (Natural product library)756530.078125
0.1186.30E-20SGTC_2734clemastine 41.6 μMMiscellaneous269870.108108ERG2
0.1164.09E-19SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.0684932mitochondrial processes
0.1155.68E-19SGTC_1081sertraline 6.0 μMNIH Clinical Collection630090.12121260S ribosome export
0.1157.63E-19SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.12307760S ribosome export
0.1101.90E-17SGTC_9541189-0853 2.9 μMChemDiv (Drug-like library)28281940.0746269endomembrane recycling
0.1094.54E-17SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.0634921cell wall signaling
0.1088.59E-17SGTC_9453381-0768 11.3 μMChemDiv (Drug-like library)11262360.193548endomembrane recycling
0.1062.67E-16SGTC_1993st073995 70.8 μMTimTec (Natural product derivative library)30983940.116883
0.0984.65E-14SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0806452heme biosynthesis & mitochondrial translocase
0.0961.08E-13SGTC_8530419-0306 21.0 μMChemDiv (Drug-like library)7627130.106061

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1094duloxetine25.4 μM160834NIH Clinical Collection333.87554.22823RPP1 & pyrimidine depletion
SGTC_2771fluoxetine41.05 μM0.3333333386Miscellaneous309.326134.03315
SGTC_2722naftopidil47.16 μM0.2686574418Miscellaneous392.490723.96215
SGTC_20535265679138 μM0.258621764595Chembridge (Fragment library)233.286242.55113
SGTC_22037227154166.4 μM0.254545896304Chembridge (Fragment library)202.249062.46612
SGTC_1280906-298113.37 μM0.2424246740226ChemDiv (Drug-like library)296.343682.0124
SGTC_12340326-049415.5 μM0.2419351547813ChemDiv (Drug-like library)268.333582.80213
SGTC_1982st07246771.9 μM0.2419353163533TimTec (Natural product derivative library)278.32513.99803
SGTC_3187910939449.47 μM0.2187517098090Chembridge (Drug-like library)247.312822.913Golgi
SGTC_2736butenafine14.65 μM0.2131152484Miscellaneous317.467185.79501