galangin

3,5,7-trihydroxy-2-phenylchromen-4-one

Galangin is a flavonoid found in medicinal herbs. An inhibitor of cancer cell proliferation, galangin is used to study mechanisms of genotoxicity.

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PubChem MeSH terms: Mutagens



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1115
Screen concentration 695.7 nM
Source TimTec (Natural product library)
PubChem CID 5281616
SMILES C1=CC=C(C=C1)C2=C(C(=O)C3=C(C=C(C=C3O2)O)O)O
Standardized SMILES OC1=C(Oc2cc(O)cc(O)c2C1=O)c3ccccc3
Molecular weight 270.2369
ALogP 2.11
H-bond donor count 3
H-bond acceptor count 5
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.71
% growth inhibition (Hom. pool) 3.54


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5281616
Download HIP data (tab-delimited text)  (excel)
Gene:CDC60(YPL160W)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.05||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:GRC3(YLL035W)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.1||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:IDI1(YPL117C)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.12||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:PCM1(YEL058W)|FD-Score:4.23|P-value:1.17E-5|Clearance:0.75||SGD DESC:Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors Gene:RHO1(YPR165W)|FD-Score:5.87|P-value:2.18E-9|Clearance:1.64||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPN6(YDL097C)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.04||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:8.68|P-value:1.99E-18|Clearance:2.81||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC61(YLR378C)|FD-Score:3.16|P-value:7.81E-4|Clearance:0.19||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:TOM22(YNL131W)|FD-Score:-3.85|P-value:5.85E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:CDC60(YPL160W)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.05||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:GRC3(YLL035W)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.1||SGD DESC:Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated Gene:IDI1(YPL117C)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.12||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:PCM1(YEL058W)|FD-Score:4.23|P-value:1.17E-5|Clearance:0.75||SGD DESC:Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors Gene:RHO1(YPR165W)|FD-Score:5.87|P-value:2.18E-9|Clearance:1.64||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPN6(YDL097C)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.04||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPP1(YHR062C)|FD-Score:8.68|P-value:1.99E-18|Clearance:2.81||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC61(YLR378C)|FD-Score:3.16|P-value:7.81E-4|Clearance:0.19||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:TOM22(YNL131W)|FD-Score:-3.85|P-value:5.85E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5281616
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADH6(YMR318C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ARG81(YML099C)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ARN1(YHL040C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress Gene:CPR2(YHR057C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:DAP1(YPL170W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:ECM5(YMR176W)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:ERV15(YBR210W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FIN1(YDR130C)|FD-Score:4.34|P-value:7.02E-6||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FKH2(YNL068C)|FD-Score:3.74|P-value:9.34E-5||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:GEA2(YEL022W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GRX8(YLR364W)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic Gene:GSH2(YOL049W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:LSC2(YGR244C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAK10(YEL053C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAK3(YPR051W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MBF1(YOR298C-A)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MHF2(YDL160C-A)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MSS1(YMR023C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NAM8(YHR086W)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NRG2(YBR066C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OAC1(YKL120W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OCA2(YNL056W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:ODC2(YOR222W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PET127(YOR017W)|FD-Score:4.87|P-value:5.66E-7||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PHO89(YBR296C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PPS1(YBR276C)|FD-Score:3.8|P-value:7.24E-5||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:PPZ1(YML016C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PSY4(YBL046W)|FD-Score:4.41|P-value:5.22E-6||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:RAD23(YEL037C)|FD-Score:3.75|P-value:9.01E-5||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RAD33(YML011C)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:THI74(YDR438W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:VPS5(YOR069W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS61(YDR136C_d)|FD-Score:5.01|P-value:2.77E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL096C(YBL096C_d)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Non-essential protein of unknown function Gene:YBR225W(YBR225W_p)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YCR015C(YCR015C_p)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR023C(YCR023C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YCT1(YLL055W)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YDR209C(YDR209C_d)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YDR514C(YDR514C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YFL019C(YFL019C_d)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR151C(YGR151C_d)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHR210C(YHR210C_p)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YKL050C(YKL050C)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p Gene:YNL193W(YNL193W_p)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOR316C-A(YOR316C-A_p)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR378W(YOR378W_p)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene Gene:YPR108W-A(YPR108W-A_p)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:ZRG17(YNR039C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency Gene:AAP1(YHR047C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADH6(YMR318C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ARG81(YML099C)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ARN1(YHL040C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress Gene:CPR2(YHR057C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:DAP1(YPL170W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:ECM5(YMR176W)|FD-Score:4.4|P-value:5.53E-6||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:ERV15(YBR210W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FIN1(YDR130C)|FD-Score:4.34|P-value:7.02E-6||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FKH2(YNL068C)|FD-Score:3.74|P-value:9.34E-5||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:GEA2(YEL022W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GRX8(YLR364W)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic Gene:GSH2(YOL049W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:LSC2(YGR244C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAK10(YEL053C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAK3(YPR051W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MBF1(YOR298C-A)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress Gene:MHF2(YDL160C-A)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MSS1(YMR023C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NAM8(YHR086W)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NRG2(YBR066C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OAC1(YKL120W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OCA2(YNL056W)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:ODC2(YOR222W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PET127(YOR017W)|FD-Score:4.87|P-value:5.66E-7||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PHO89(YBR296C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PPS1(YBR276C)|FD-Score:3.8|P-value:7.24E-5||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:PPZ1(YML016C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PSY4(YBL046W)|FD-Score:4.41|P-value:5.22E-6||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:RAD23(YEL037C)|FD-Score:3.75|P-value:9.01E-5||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RAD33(YML011C)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:THI74(YDR438W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:VPS5(YOR069W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS61(YDR136C_d)|FD-Score:5.01|P-value:2.77E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL096C(YBL096C_d)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Non-essential protein of unknown function Gene:YBR225W(YBR225W_p)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YCR015C(YCR015C_p)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR023C(YCR023C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YCT1(YLL055W)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YDR209C(YDR209C_d)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YDR514C(YDR514C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YFL019C(YFL019C_d)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR151C(YGR151C_d)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHR210C(YHR210C_p)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YKL050C(YKL050C)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p Gene:YNL193W(YNL193W_p)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOR316C-A(YOR316C-A_p)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR378W(YOR378W_p)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene Gene:YPR108W-A(YPR108W-A_p)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:ZRG17(YNR039C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C8.681.99E-182.81RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPR165W5.872.18E-91.64RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YEL058W4.231.17E-50.75PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YPL117C3.482.54E-40.12IDI1Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability
YDL097C3.353.98E-40.04RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YPL160W3.314.64E-40.05CDC60Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
YLL035W3.265.61E-40.10GRC3Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
YLR378C3.167.81E-40.19SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YLR198C_d2.970.001470.07YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YER012W2.910.001830.11PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YKL152C2.800.002580.06GPM1Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
YER133W2.740.003050.05GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YNL251C2.690.003550.01NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YER168C2.690.003610.03CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YGR158C2.660.003890.16MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR136C_d5.012.77E-7VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YOR017W4.875.66E-7PET127Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane
YML011C4.602.12E-6RAD33Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
YBL046W4.415.22E-6PSY4Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2
YMR176W4.405.53E-6ECM5Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress
YDR130C4.347.02E-6FIN1Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress
YPR051W4.241.14E-5MAK3Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus
YHL040C4.171.55E-5ARN1ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
YPL170W4.121.92E-5DAP1Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
YLR364W4.111.94E-5GRX8Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic
YCR023C4.102.08E-5YCR023CVacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene
YPR108W-A_p4.013.06E-5YPR108W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YLL055W3.993.37E-5YCT1High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene
YKL050C3.953.97E-5YKL050CProtein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p
YBR225W_p3.924.38E-5YBR225W_pPutative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components

GO enrichment analysis for SGTC_1115
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1432.41E-28SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.0945946RPP1 & pyrimidine depletion
0.1432.73E-28SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0684932RPP1 & pyrimidine depletion
0.1285.70E-23SGTC_1101ebselen 5.3 μMNIH Clinical Collection31940.16
0.1262.07E-22SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.0945946RNA pol III & RNase P/MRP
0.1133.98E-18SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.0933333cell wall
0.1041.22E-15SGTC_3620310-0100 9.0 μMChemDiv (Drug-like library)7902350.142857RPP1 & pyrimidine depletion
0.1024.49E-15SGTC_24984'-Methoxychalcone 54.6 μMMicrosource (Natural product library)6418180.125RPP1 & pyrimidine depletion
0.1019.72E-15SGTC_1642st009582 77.7 μMTimTec (Natural product derivative library)67393110.175439RPP1 & pyrimidine depletion
0.1001.31E-14SGTC_13611608-0041 5.6 μMChemDiv (Drug-like library)X13610.0634921RPP1 & pyrimidine depletion
0.1001.67E-14SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.112676RPP1 & pyrimidine depletion
0.0976.11E-14SGTC_15346-azauridine 81.6 μMTimTec (Pure natural product library)2335020.0952381RNA pol III & RNase P/MRP
0.0961.35E-13SGTC_14853909-9022 254.0 μMChemDiv (Drug-like library)7145160.0428571RPP1 & pyrimidine depletion
0.0938.55E-13SGTC_1320417-1715 25.8 μMChemDiv (Drug-like library)67690980.130435RPP1 & pyrimidine depletion
0.0921.26E-12SGTC_33229139058 2.1 μMChembridge (Drug-like library)49089260.121622cell wall
0.0921.36E-12SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.107692

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2505galangin7.89 μM15281616Microsource (Natural product library)270.23692.11435RPP1 & pyrimidine depletion
SGTC_1562morin hydrate59.1 μM0.6518542136TimTec (Pure natural product library)338.266261.21679
SGTC_2515chrysin20.77 μM0.5714295281607Microsource (Natural product library)254.23752.65224RPP1 & pyrimidine depletion
SGTC_26335,7-dihydroxyisoflavone100 μM0.5714295377381Microsource (Natural product library)254.23752.38224
SGTC_1109tnp00067928.26 nM0.5676295TimTec (Natural product library)254.23752.35624
SGTC_1112tnp000691.17 μM0.466667145826TimTec (Natural product library)270.23692.11435
SGTC_15823',3',6'-trihydroxyflavone74 μM0.466667688803TimTec (Pure natural product library)270.23692.11435
SGTC_1124fisetin1.16 μM0.4565225281614TimTec (Natural product library)286.23631.87246
SGTC_1529apigenin74 μM0.4444445280443TimTec (Pure natural product library)270.23692.4135
SGTC_11103-hydroxy-3'-methoxyflavone1.24 μM0.42676296TimTec (Natural product library)268.264082.58214iron homeostasis