retinoic acid FDA approved compound

(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenoic acid

A transcription inhibitor binds retinoic acid receptor (RAR), which is bound to DNA as a heterodimer with the retinoid X receptor (RXR).

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1117
Screen concentration 1.1 μM
Source TimTec (Natural product library)
PubChem CID 444795
SMILES CC1=C(C(CCC1)(C)C)C=CC(=CC=CC(=CC(=O)O)C)C
Standardized SMILES CC(=CC=CC(=CC(=O)O)C)C=CC1=C(C)CCCC1(C)C
Molecular weight 300.4351
ALogP 5.55
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.93
% growth inhibition (Hom. pool) -1.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 444795
Download HIP data (tab-delimited text)  (excel)
Gene:HYP2(YEL034W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.22||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:RPP0(YLR340W)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.22||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SRB4(YER022W)|FD-Score:3.31|P-value:4.73E-4|Clearance:0.2||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TOP2(YNL088W)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.07||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:HYP2(YEL034W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.22||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:RPP0(YLR340W)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.22||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:SRB4(YER022W)|FD-Score:3.31|P-value:4.73E-4|Clearance:0.2||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TOP2(YNL088W)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.07||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 444795
Download HOP data (tab-delimited text)  (excel)
Gene:ADE5,7(YGL234W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AIM26(YKL037W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:APP1(YNL094W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARG82(YDR173C)|FD-Score:5.29|P-value:6.01E-8||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG11(YPR049C)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:COG6(YNL041C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRG1(YHR209W)|FD-Score:6.01|P-value:9.06E-10||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:GCN4(YEL009C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GIS4(YML006C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:GLC8(YMR311C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:INO2(YDR123C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:INO4(YOL108C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LYS1(YIR034C)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MHF1(YOL086W-A)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MID2(YLR332W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MNN2(YBR015C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:PER33(YLR064W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PEX28(YHR150W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PFK2(YMR205C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHM7(YOL084W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress Gene:PHO89(YBR296C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIN3(YPR154W)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PKH2(YOL100W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PSH1(YOL054W)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PTC3(YBL056W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:RAD50(YNL250W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RBG2(YGR173W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RPB9(YGL070C)|FD-Score:-4.67|P-value:1.52E-6||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPN4(YDL020C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC3(YDR159W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAW1(YAL027W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SET5(YHR207C)|FD-Score:5.09|P-value:1.83E-7||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SOD1(YJR104C)|FD-Score:5.74|P-value:4.78E-9||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOP4(YJL192C)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO77(YLR341W)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:TGL3(YMR313C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TPS2(YDR074W)|FD-Score:5.04|P-value:2.39E-7||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:8.34|P-value:3.80E-17||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:7.44|P-value:5.14E-14||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUS1(YLR425W)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBC13(YDR092W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:VMA11(YPL234C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS45(YGL095C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YDL176W(YDL176W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR431W(YDR431W_d)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR202W(YHR202W_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YKE2(YLR200W)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YMR057C(YMR057C_d)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YNL195C(YNL195C_p)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR147C(YPR147C_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:ADE5,7(YGL234W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AIM26(YKL037W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:APP1(YNL094W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ARG82(YDR173C)|FD-Score:5.29|P-value:6.01E-8||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG11(YPR049C)|FD-Score:-3.23|P-value:6.16E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:COG6(YNL041C)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRG1(YHR209W)|FD-Score:6.01|P-value:9.06E-10||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:GCN4(YEL009C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GIS4(YML006C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway Gene:GLC8(YMR311C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:INO2(YDR123C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:INO4(YOL108C)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LYS1(YIR034C)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MHF1(YOL086W-A)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MID2(YLR332W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MNN2(YBR015C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:PER33(YLR064W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PEX28(YHR150W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PFK2(YMR205C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHM7(YOL084W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress Gene:PHO89(YBR296C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIN3(YPR154W)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PKH2(YOL100W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PSH1(YOL054W)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PTC3(YBL056W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:RAD50(YNL250W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress Gene:RBG2(YGR173W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RPB9(YGL070C)|FD-Score:-4.67|P-value:1.52E-6||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPN4(YDL020C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC3(YDR159W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAW1(YAL027W)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SET5(YHR207C)|FD-Score:5.09|P-value:1.83E-7||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SOD1(YJR104C)|FD-Score:5.74|P-value:4.78E-9||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOP4(YJL192C)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPO77(YLR341W)|FD-Score:-3.12|P-value:9.08E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:TGL3(YMR313C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TPS2(YDR074W)|FD-Score:5.04|P-value:2.39E-7||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:8.34|P-value:3.80E-17||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:7.44|P-value:5.14E-14||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUS1(YLR425W)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBC13(YDR092W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:VMA11(YPL234C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPS45(YGL095C)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YDL176W(YDL176W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR431W(YDR431W_d)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR202W(YHR202W_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YKE2(YLR200W)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YMR057C(YMR057C_d)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YNL195C(YNL195C_p)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR147C(YPR147C_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YEL034W3.671.21E-40.22HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YLR340W3.522.13E-40.22RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YER022W3.314.73E-40.20SRB4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
YNL088W3.119.47E-40.07TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YMR270C3.040.001190.09RRN9Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
YOR057W2.950.001580.02SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
YPR112C2.930.001700.14MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YJL109C2.790.002660.05UTP10Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
YJL111W2.730.003140.04CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YFR027W2.690.003570.06ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
YGL150C2.630.004330.03INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YLL035W2.600.004710.02GRC3Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
YJL002C2.580.005000.00OST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YDR327W_d2.570.005070.02YDR327W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
YPL238C_d2.550.005380.01YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C8.343.80E-17TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YBR058C-A7.445.14E-14TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YHR209W6.019.06E-10CRG1S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
YJR104C5.744.78E-9SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YDR173C5.296.01E-8ARG82Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
YHR207C5.091.83E-7SET5Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
YDR074W5.042.39E-7TPS2Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress
YMR313C4.731.12E-6TGL3Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation
YDR354W4.721.18E-6TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YGL095C4.241.12E-5VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YAL027W4.201.34E-5SAW15'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
YDR007W4.181.48E-5TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDL176W4.062.45E-5YDL176WProtein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
YMR311C3.885.16E-5GLC8Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
YIR034C3.797.64E-5LYS1Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity

GO enrichment analysis for SGTC_1117
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3659.88E-186SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.104478TSC3-RPN4
0.3158.50E-136SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.105263
0.2541.51E-87SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.0933333
0.2524.02E-86SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.0649351
0.2471.04E-82SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0615385
0.2465.22E-82SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.0769231TSC3-RPN4
0.2431.93E-80SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0722892TSC3-RPN4
0.2421.48E-79SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.104478TSC3-RPN4
0.2411.38E-78SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.0547945
0.2393.04E-77SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0153846
0.2357.44E-75SGTC_24885268135 45.7 μMMiscellaneous12703560.0735294
0.2344.96E-74SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0740741
0.2301.52E-71SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0963855
0.2263.73E-69SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.128571
0.2221.03E-66SGTC_18825615643 20.0 μMMiscellaneous22530750.0581395TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2670beta-carotene100 μM0.7619055280489Microsource (Natural product library)536.8726411.99800
SGTC_2646retinol96.75 μM0.6875445354Microsource (Natural product library)286.45165.31611
SGTC_2552juarezic acid87.02 μM0.2037041549512Microsource (Natural product library)174.19592.39512TSC3-RPN4
SGTC_2749bexarotene57.39 μM0.20312582146Miscellaneous348.477926.40512
SGTC_2961222-003974.9 μM0.25984176ChemDiv (Drug-like library)218.291524.22812
SGTC_9591222-0044107 μM0.1967216083612ChemDiv (Drug-like library)232.31814.68512
SGTC_9581222-000733 μM0.1935487312461ChemDiv (Drug-like library)246.344685.14112
SGTC_2587piperic acid92.2 μM0.1935485370536Microsource (Natural product library)218.20542.16314
SGTC_2571chrysanthemic acid100 μM0.1851852743Microsource (Natural product library)168.232842.17712
SGTC_2750bromebric acid70.15 μM0.1833335358572Miscellaneous285.090762.24914