chelidonine

Benzophenanthridine derived from scoulerine from Chelidonium majus; putative GST and MDR inhibitor.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1118
Screen concentration 707.5 nM
Source TimTec (Natural product library)
PubChem CID 10147
SMILES CN1CC2=C(C=CC3=C2OCO3)C4C1C5=CC6=C(C=C5CC4O)OCO6
Standardized SMILES CN1Cc2c3OCOc3ccc2C4C(O)Cc5cc6OCOc6cc5C14
Molecular weight 353.3686
ALogP 2.33
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -4.14


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 10147
Download HIP data (tab-delimited text)  (excel)
Gene:CCA1(YER168C)|FD-Score:3.82|P-value:6.60E-5|Clearance:0.25||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC7(YDL017W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.03||SGD DESC:DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p Gene:HSH49(YOR319W)|FD-Score:4.36|P-value:6.56E-6|Clearance:0.54||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:ILV5(YLR355C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.07||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:ORC4(YPR162C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.23||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PFY1(YOR122C)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.14||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:SEC8(YPR055W)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.05||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SWP1(YMR149W)|FD-Score:3.27|P-value:5.43E-4|Clearance:0.11||SGD DESC:Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YJR023C(YJR023C_d)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.02||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing Gene:CCA1(YER168C)|FD-Score:3.82|P-value:6.60E-5|Clearance:0.25||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDC7(YDL017W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.03||SGD DESC:DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p Gene:HSH49(YOR319W)|FD-Score:4.36|P-value:6.56E-6|Clearance:0.54||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:ILV5(YLR355C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.07||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:ORC4(YPR162C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.23||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PFY1(YOR122C)|FD-Score:3.52|P-value:2.13E-4|Clearance:0.14||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:SEC8(YPR055W)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.05||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SWP1(YMR149W)|FD-Score:3.27|P-value:5.43E-4|Clearance:0.11||SGD DESC:Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YJR023C(YJR023C_d)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.02||SGD DESC:Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 10147
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:AAD4(YDL243C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ANS1(YHR126C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARL1(YBR164C)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASI1(YMR119W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:AYR1(YIL124W)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BIO4(YNR057C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BSC5(YNR069C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CIN8(YEL061C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CPR6(YLR216C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:EAF7(YNL136W)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ECL1(YGR146C)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ECM22(YLR228C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ERG3(YLR056W)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FRD1(YEL047C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GCN5(YGR252W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GSF2(YML048W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HMS1(YOR032C)|FD-Score:5.5|P-value:1.90E-8||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HOP1(YIL072W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HPA3(YEL066W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:KEL3(YPL263C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:KIN1(YDR122W)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:LIP2(YLR239C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MSH2(YOL090W)|FD-Score:4.43|P-value:4.60E-6||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSH5(YDL154W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MSM1(YGR171C)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MTC4(YBR255W)|FD-Score:-4.59|P-value:2.21E-6||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NTO1(YPR031W)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:NUP120(YKL057C)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OAC1(YKL120W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OSH6(YKR003W)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PIH1(YHR034C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PIM1(YBL022C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PTH1(YHR189W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RGL1(YPL066W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RHB1(YCR027C)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPL14A(YKL006W)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:SAC7(YDR389W)|FD-Score:-4.58|P-value:2.34E-6||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAM37(YMR060C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAT4(YCR008W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SLZ1(YNL196C_p)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNZ2(YNL333W)|FD-Score:5.24|P-value:7.96E-8||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SRO9(YCL037C)|FD-Score:-4.11|P-value:1.97E-5||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRS2(YJL092W)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:TDA3(YHR009C_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:THI6(YPL214C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:THP2(YHR167W)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TRK2(YKR050W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TYW1(YPL207W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:VAC14(YLR386W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS38(YLR360W)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:YAP1(YML007W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR277C(YBR277C_d)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCP4(YCR004C)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR102W-A(YCR102W-A_d)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR242W(YGR242W_d)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR095W(YHR095W_d)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL030W(YKL030W_d)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKR005C(YKR005C_p)|FD-Score:5.64|P-value:8.67E-9||SGD DESC:Putative protein of unknown function Gene:YLL053C(YLL053C_p)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR050C(YLR050C_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YLR236C(YLR236C_d)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR402W(YLR402W_d)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML079W(YML079W_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YMR310C(YMR310C_p)|FD-Score:5.91|P-value:1.69E-9||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNR062C(YNR062C_p)|FD-Score:5.07|P-value:2.04E-7||SGD DESC:Putative membrane protein of unknown function Gene:YOL013W-A(YOL013W-A_p)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOR318C(YOR318C_d)|FD-Score:4.8|P-value:7.80E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-5.56|P-value:1.38E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YSC83(YHR017W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:AAC1(YMR056C)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:AAD4(YDL243C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ANS1(YHR126C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ARL1(YBR164C)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASI1(YMR119W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:AYR1(YIL124W)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BIO4(YNR057C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BSC5(YNR069C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CIN8(YEL061C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CPR6(YLR216C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:EAF7(YNL136W)|FD-Score:-3.19|P-value:7.17E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ECL1(YGR146C)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ECM22(YLR228C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ERG3(YLR056W)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FRD1(YEL047C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:GCN5(YGR252W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GSF2(YML048W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HMS1(YOR032C)|FD-Score:5.5|P-value:1.90E-8||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HOP1(YIL072W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HPA3(YEL066W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:KEL3(YPL263C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:KIN1(YDR122W)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:LIP2(YLR239C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MSH2(YOL090W)|FD-Score:4.43|P-value:4.60E-6||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSH5(YDL154W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MSM1(YGR171C)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MTC4(YBR255W)|FD-Score:-4.59|P-value:2.21E-6||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NTO1(YPR031W)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:NUP120(YKL057C)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OAC1(YKL120W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Mitochondrial inner membrane transporter, transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family Gene:OSH6(YKR003W)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PIH1(YHR034C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PIM1(YBL022C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PTH1(YHR189W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RGL1(YPL066W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RHB1(YCR027C)|FD-Score:-4.03|P-value:2.79E-5||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RPL14A(YKL006W)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:SAC7(YDR389W)|FD-Score:-4.58|P-value:2.34E-6||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAM37(YMR060C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAT4(YCR008W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SLZ1(YNL196C_p)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNZ2(YNL333W)|FD-Score:5.24|P-value:7.96E-8||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SRO9(YCL037C)|FD-Score:-4.11|P-value:1.97E-5||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRS2(YJL092W)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:TDA3(YHR009C_p)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:THI6(YPL214C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:THP2(YHR167W)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TRK2(YKR050W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; TRK2 has a paralog, TRK1, that arose from the whole genome duplication Gene:TYW1(YPL207W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:VAC14(YLR386W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS38(YLR360W)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:YAP1(YML007W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR277C(YBR277C_d)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCP4(YCR004C)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR102W-A(YCR102W-A_d)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR242W(YGR242W_d)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR095W(YHR095W_d)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL030W(YKL030W_d)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKR005C(YKR005C_p)|FD-Score:5.64|P-value:8.67E-9||SGD DESC:Putative protein of unknown function Gene:YLL053C(YLL053C_p)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR050C(YLR050C_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene Gene:YLR236C(YLR236C_d)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR402W(YLR402W_d)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML079W(YML079W_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YMR310C(YMR310C_p)|FD-Score:5.91|P-value:1.69E-9||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNR062C(YNR062C_p)|FD-Score:5.07|P-value:2.04E-7||SGD DESC:Putative membrane protein of unknown function Gene:YOL013W-A(YOL013W-A_p)|FD-Score:-3.1|P-value:9.78E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOR318C(YOR318C_d)|FD-Score:4.8|P-value:7.80E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-5.56|P-value:1.38E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YSC83(YHR017W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR319W4.366.56E-60.54HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YER168C3.826.60E-50.25CCA1ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
YPR055W3.581.74E-40.05SEC8Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
YOR122C3.522.13E-40.14PFY1Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress
YJR023C_d3.383.57E-40.02YJR023C_dPutative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
YDL017W3.363.85E-40.03CDC7DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p
YLR355C3.334.27E-40.07ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YMR149W3.275.43E-40.11SWP1Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum
YPR162C3.167.85E-40.23ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YPR103W2.930.001680.06PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YJL015C_d2.880.002010.09YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
YKL144C2.790.002620.07RPC25RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
YGR264C2.730.003210.01MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YLR088W2.720.003300.03GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YPL218W2.690.003560.08SAR1GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR310C_p5.911.69E-9YMR310C_pPutative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene
YKR005C_p5.648.67E-9YKR005C_pPutative protein of unknown function
YLL053C_p5.611.02E-8YLL053C_pPutative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin
YKL006W5.611.02E-8RPL14ARibosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
YOR032C5.501.90E-8HMS1Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant
YNL333W5.247.96E-8SNZ2Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p
YNR062C_p5.072.04E-7YNR062C_pPutative membrane protein of unknown function
YKR003W4.856.12E-7OSH6Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion
YOR318C_d4.807.80E-7YOR318C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo
YCR102W-A_d4.503.39E-6YCR102W-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YCR004C4.454.38E-6YCP4Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YOL090W4.434.60E-6MSH2Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
YIL124W4.415.10E-6AYR1NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones
YHR167W4.415.24E-6THP2Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
YLR216C4.201.34E-5CPR6Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1118
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0681.43E-7SGTC_2593nobiletin 86.2 μMMicrosource (Natural product library)723440.0933333
0.0672.55E-7SGTC_32589138477 49.5 μMChembridge (Drug-like library)49703550.0869565
0.0534.37E-5SGTC_23186029776 164.0 μMChembridge (Fragment library)8183250.0555556
0.0511.00E-4SGTC_1106tegaserod maleate 11.7 μMNIH Clinical Collection69183690.0555556
0.0511.03E-4SGTC_21225258151 111.7 μMChembridge (Fragment library)21733560.0526316
0.0501.31E-4SGTC_1690st023502 41.2 μMTimTec (Natural product derivative library)242074820.102273calcium & mitochondrial duress
0.0473.30E-4SGTC_1949st076024 69.1 μMTimTec (Natural product derivative library)29219300.0843373
0.0463.62E-4SGTC_2718lomofungin 53.0 μMMiscellaneous53512220.0779221mitochondrial processes
0.0463.64E-4SGTC_20445102272 200.0 μMChembridge (Fragment library)5926660.109375
0.0464.08E-4SGTC_21475545932 89.7 μMChembridge (Fragment library)7974040.0895522ergosterol biosynthesis
0.0454.77E-4SGTC_15004488-1866 171.0 μMChemDiv (Drug-like library)29000030.119565RPP1 & pyrimidine depletion
0.0454.90E-4SGTC_282k297-0036 65.7 μMChemDiv (Drug-like library)50613780.0493827
0.0454.93E-4SGTC_21145491910 81.2 μMChembridge (Fragment library)28492280.142857
0.0456.10E-4SGTC_31549100113 49.5 μMChembridge (Drug-like library)252375250.0833333
0.0446.35E-4SGTC_31599100096 49.5 μMChembridge (Drug-like library)252368750.0789474

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1718roemerine11.93 μM0.313433235224TimTec (Natural product derivative library)279.333083.36803redox potentiating
SGTC_2413inermin26.06 μM0.26562591510TimTec (Natural product derivative library)284.263482.46715
SGTC_2659sanguinarine24.21 μM0.26153813394149Microsource (Natural product library)429.41.13518
SGTC_1648st01173669.8 μM0.2318842855514TimTec (Natural product derivative library)286.365483.57603PDR1
SGTC_13661683-011321.9 μM0.222222708623ChemDiv (Drug-like library)224.233121.75925
SGTC_1845st0567533.01 μM0.218755375839TimTec (Natural product derivative library)207.182762.09104TSC3-RPN4
SGTC_2587piperic acid92.2 μM0.218755370536Microsource (Natural product library)218.20542.16314
SGTC_1781st03872566.3 μM0.2089554270275TimTec (Natural product derivative library)301.767443.50413NEO1
SGTC_10472043-304482 μM0.208333669592ChemDiv (Drug-like library)323.385643.66903
SGTC_7473448-808738.7 μM0.2051285168703ChemDiv (Drug-like library)441.907362.99606