parthenolide

Parthenolide specifically inhibits HDAC1 (histone deacetylase 1) without affecting other class I/II HDACs.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1119
Screen concentration 318.5 nM
Source TimTec (Natural product library)
PubChem CID 5702252
SMILES CC1=CCCC2(C(O2)C3C(CC1)C(=C)C(=O)O3)C
Standardized SMILES CC1=CCCC2(C)OC2C3OC(=O)C(=C)C3CC1
Molecular weight 248.3175
ALogP 2.92
H-bond donor count 0
H-bond acceptor count 3
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.46
% growth inhibition (Hom. pool) 2.7


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5702252
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:-3.27|P-value:5.29E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CNS1(YBR155W)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.01||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:GCD14(YJL125C)|FD-Score:4.12|P-value:1.85E-5|Clearance:0.38||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:HYM1(YKL189W)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.03||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:MET30(YIL046W)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.2||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:PDC2(YDR081C)|FD-Score:-5.28|P-value:6.55E-8|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRE6(YOL038W)|FD-Score:4.68|P-value:1.41E-6|Clearance:0.19||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RHO1(YPR165W)|FD-Score:3.24|P-value:5.91E-4|Clearance:0.05||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPP1(YHR062C)|FD-Score:5.99|P-value:1.04E-9|Clearance:1.14||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS20(YHL015W)|FD-Score:7.18|P-value:3.58E-13|Clearance:1.14||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RTT105(YER104W)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.02||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC31(YDL195W)|FD-Score:6.2|P-value:2.79E-10|Clearance:1.14||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC4(YFL005W)|FD-Score:5.85|P-value:2.40E-9|Clearance:1.14||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SEC8(YPR055W)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.16||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SRM1(YGL097W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.15||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:TOM22(YNL131W)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.12||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRS20(YBR254C)|FD-Score:4.72|P-value:1.19E-6|Clearance:0.04||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:TUB2(YFL037W)|FD-Score:4.49|P-value:3.57E-6|Clearance:0.35||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YPP1(YGR198W)|FD-Score:-3.53|P-value:2.11E-4|Clearance:0||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:BMS1(YPL217C)|FD-Score:-3.27|P-value:5.29E-4|Clearance:0||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CNS1(YBR155W)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.01||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:GCD14(YJL125C)|FD-Score:4.12|P-value:1.85E-5|Clearance:0.38||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:HYM1(YKL189W)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.03||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:MET30(YIL046W)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.2||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:PDC2(YDR081C)|FD-Score:-5.28|P-value:6.55E-8|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRE6(YOL038W)|FD-Score:4.68|P-value:1.41E-6|Clearance:0.19||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RHO1(YPR165W)|FD-Score:3.24|P-value:5.91E-4|Clearance:0.05||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPP1(YHR062C)|FD-Score:5.99|P-value:1.04E-9|Clearance:1.14||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS20(YHL015W)|FD-Score:7.18|P-value:3.58E-13|Clearance:1.14||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RTT105(YER104W)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.02||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC31(YDL195W)|FD-Score:6.2|P-value:2.79E-10|Clearance:1.14||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC4(YFL005W)|FD-Score:5.85|P-value:2.40E-9|Clearance:1.14||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SEC8(YPR055W)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.16||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SRM1(YGL097W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.15||SGD DESC:Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p Gene:TOM22(YNL131W)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.12||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TRS20(YBR254C)|FD-Score:4.72|P-value:1.19E-6|Clearance:0.04||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:TUB2(YFL037W)|FD-Score:4.49|P-value:3.57E-6|Clearance:0.35||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YPP1(YGR198W)|FD-Score:-3.53|P-value:2.11E-4|Clearance:0||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5702252
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADE12(YNL220W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:ADE8(YDR408C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ALF1(YNL148C)|FD-Score:6.06|P-value:6.80E-10||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ATG11(YPR049C)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BAP3(YDR046C)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BIO5(YNR056C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CAP1(YKL007W)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CAP2(YIL034C)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCS1(YMR038C)|FD-Score:4.83|P-value:6.75E-7||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHS5(YLR330W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CIT1(YNR001C)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CSF1(YLR087C)|FD-Score:7.41|P-value:6.48E-14||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DCC1(YCL016C)|FD-Score:-4.43|P-value:4.80E-6||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DPB4(YDR121W)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:ELF1(YKL160W)|FD-Score:4.86|P-value:5.81E-7||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:ENV10(YLR065C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ENV9(YOR246C)|FD-Score:4.91|P-value:4.66E-7||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:GAL11(YOL051W)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAS4(YOL132W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GCN4(YEL009C)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDT1(YBR187W_p)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GLE2(YER107C)|FD-Score:5.65|P-value:8.12E-9||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GMC2(YLR445W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GND2(YGR256W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HFI1(YPL254W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HOM6(YJR139C)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IMP2(YMR035W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IRS4(YKR019C)|FD-Score:6.34|P-value:1.13E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST3(YIR005W)|FD-Score:6.65|P-value:1.42E-11||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:JJJ3(YJR097W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:LPP1(YDR503C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:LST4(YKL176C)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAK32(YCR019W)|FD-Score:5.29|P-value:6.27E-8||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MDG1(YNL173C)|FD-Score:4.87|P-value:5.58E-7||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MDM35(YKL053C-A)|FD-Score:-3.73|P-value:9.66E-5||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MEP2(YNL142W)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET6(YER091C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:MIR1(YJR077C)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MRPL22(YNL177C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MUM3(YOR298W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:PAU11(YGL261C_p)|FD-Score:-6.32|P-value:1.35E-10||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PAU13(YHL046C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PCK1(YKR097W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PER1(YCR044C)|FD-Score:6.14|P-value:4.21E-10||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PGD1(YGL025C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHA2(YNL316C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PHO84(YML123C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PKR1(YMR123W)|FD-Score:-5.09|P-value:1.78E-7||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PUS2(YGL063W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RAD54(YGL163C)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RBL2(YOR265W)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:REV3(YPL167C)|FD-Score:-4.6|P-value:2.08E-6||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGP1(YDR137W)|FD-Score:5.71|P-value:5.74E-9||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM4(YDR257C)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RNH202(YDR279W)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPN4(YDL020C)|FD-Score:6.14|P-value:4.11E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS24A(YER074W)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RSC2(YLR357W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RSF1(YMR030W)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:RTF1(YGL244W)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTG1(YOL067C)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTR1(YER139C)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:RVS161(YCR009C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAM2(YDR502C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SAY1(YGR263C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SEA4(YBL104C)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SNF7(YLR025W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOH1(YGL127C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SOK2(YMR016C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication Gene:SSA2(YLL024C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STP22(YCL008C)|FD-Score:-7.54|P-value:2.35E-14||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUS1(YBR111W-A)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TEL1(YBL088C)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TOM7(YNL070W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRM732(YMR259C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRP2(YER090W)|FD-Score:5.22|P-value:9.06E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:9.47|P-value:1.45E-21||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:14.4|P-value:5.19E-47||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UPS1(YLR193C)|FD-Score:4.48|P-value:3.76E-6||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:VBA2(YBR293W)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS24(YKL041W)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YBL083C(YBL083C_d)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR116C(YBR116C_d)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR144C(YBR144C_d)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Gene:YCR007C(YCR007C_p)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL012C(YDL012C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:5.68|P-value:6.73E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER085C(YER085C_p)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Putative protein of unknown function Gene:YHR202W(YHR202W_p)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJL067W(YJL067W_d)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL077W-B(YJL077W-B_p)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL136W-A(YJL136W-A_p)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YJR056C(YJR056C_p)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YKU70(YMR284W)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR042C(YLR042C_p)|FD-Score:3.78|P-value:7.69E-5||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR358C(YLR358C_p)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YLR444C(YLR444C_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML009W-B(YML009W-B_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YML057C-A(YML057C-A_d)|FD-Score:7.9|P-value:1.40E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YML122C(YML122C_d)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR153C-A(YMR153C-A_d)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YMR310C(YMR310C_p)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOR034C-A(YOR034C-A_p)|FD-Score:5.52|P-value:1.66E-8||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPK1(YKL126W)|FD-Score:6.96|P-value:1.72E-12||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:AAP1(YHR047C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ADE12(YNL220W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:ADE8(YDR408C)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ALF1(YNL148C)|FD-Score:6.06|P-value:6.80E-10||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ATG11(YPR049C)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BAP3(YDR046C)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication Gene:BIO5(YNR056C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CAP1(YKL007W)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CAP2(YIL034C)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCS1(YMR038C)|FD-Score:4.83|P-value:6.75E-7||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHS5(YLR330W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CIT1(YNR001C)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CSF1(YLR087C)|FD-Score:7.41|P-value:6.48E-14||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DCC1(YCL016C)|FD-Score:-4.43|P-value:4.80E-6||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DPB4(YDR121W)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:ELF1(YKL160W)|FD-Score:4.86|P-value:5.81E-7||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:ENV10(YLR065C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ENV9(YOR246C)|FD-Score:4.91|P-value:4.66E-7||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:GAL11(YOL051W)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAS4(YOL132W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GCN4(YEL009C)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDT1(YBR187W_p)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GLE2(YER107C)|FD-Score:5.65|P-value:8.12E-9||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GMC2(YLR445W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GND2(YGR256W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HFI1(YPL254W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HOM6(YJR139C)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:IMP2(YMR035W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IRS4(YKR019C)|FD-Score:6.34|P-value:1.13E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST3(YIR005W)|FD-Score:6.65|P-value:1.42E-11||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:JJJ3(YJR097W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:LPP1(YDR503C)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:LST4(YKL176C)|FD-Score:5.92|P-value:1.58E-9||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAK32(YCR019W)|FD-Score:5.29|P-value:6.27E-8||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MDG1(YNL173C)|FD-Score:4.87|P-value:5.58E-7||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MDM35(YKL053C-A)|FD-Score:-3.73|P-value:9.66E-5||SGD DESC:Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MEP2(YNL142W)|FD-Score:-4.17|P-value:1.51E-5||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET6(YER091C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:MIR1(YJR077C)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MRPL22(YNL177C)|FD-Score:4.6|P-value:2.13E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MUM3(YOR298W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:PAU11(YGL261C_p)|FD-Score:-6.32|P-value:1.35E-10||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PAU13(YHL046C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PCK1(YKR097W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PER1(YCR044C)|FD-Score:6.14|P-value:4.21E-10||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PGD1(YGL025C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHA2(YNL316C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PHO84(YML123C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PKR1(YMR123W)|FD-Score:-5.09|P-value:1.78E-7||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PUS2(YGL063W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RAD54(YGL163C)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RBL2(YOR265W)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:REV3(YPL167C)|FD-Score:-4.6|P-value:2.08E-6||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGP1(YDR137W)|FD-Score:5.71|P-value:5.74E-9||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM4(YDR257C)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RNH202(YDR279W)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPN4(YDL020C)|FD-Score:6.14|P-value:4.11E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS24A(YER074W)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RSC2(YLR357W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RSF1(YMR030W)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:RTF1(YGL244W)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTG1(YOL067C)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTR1(YER139C)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:RVS161(YCR009C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAM2(YDR502C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SAY1(YGR263C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SEA4(YBL104C)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SNF7(YLR025W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SOH1(YGL127C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SOK2(YMR016C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication Gene:SSA2(YLL024C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:STP22(YCL008C)|FD-Score:-7.54|P-value:2.35E-14||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUS1(YBR111W-A)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TEL1(YBL088C)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TOM7(YNL070W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRM732(YMR259C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRP2(YER090W)|FD-Score:5.22|P-value:9.06E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:9.47|P-value:1.45E-21||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:14.4|P-value:5.19E-47||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UPS1(YLR193C)|FD-Score:4.48|P-value:3.76E-6||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:VBA2(YBR293W)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS24(YKL041W)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YBL083C(YBL083C_d)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR116C(YBR116C_d)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR144C(YBR144C_d)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene Gene:YCR007C(YCR007C_p)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDL012C(YDL012C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:5.68|P-value:6.73E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER085C(YER085C_p)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Putative protein of unknown function Gene:YHR202W(YHR202W_p)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJL067W(YJL067W_d)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL077W-B(YJL077W-B_p)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YJL136W-A(YJL136W-A_p)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YJR056C(YJR056C_p)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YKU70(YMR284W)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR042C(YLR042C_p)|FD-Score:3.78|P-value:7.69E-5||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR358C(YLR358C_p)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YLR444C(YLR444C_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML009W-B(YML009W-B_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YML057C-A(YML057C-A_d)|FD-Score:7.9|P-value:1.40E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YML122C(YML122C_d)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR153C-A(YMR153C-A_d)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NUP53 Gene:YMR310C(YMR310C_p)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL162W-A(YNL162W-A_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YOR034C-A(YOR034C-A_p)|FD-Score:5.52|P-value:1.66E-8||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPK1(YKL126W)|FD-Score:6.96|P-value:1.72E-12||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHL015W7.183.58E-131.14RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YDL195W6.202.79E-101.14SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YHR062C5.991.04E-91.14RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YFL005W5.852.40E-91.14SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YBR254C4.721.19E-60.04TRS20One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
YOL038W4.681.41E-60.19PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YFL037W4.493.57E-60.35TUB2Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
YBR155W4.141.75E-50.01CNS1TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
YJL125C4.121.85E-50.38GCD14Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YIL046W3.758.89E-50.20MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YKL189W3.551.96E-40.03HYM1Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response
YNL131W3.512.22E-40.12TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YGL097W3.403.43E-40.15SRM1Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
YPR165W3.245.91E-40.05RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YER104W3.197.10E-40.02RTT105Protein with a role in regulation of Ty1 transposition

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A14.405.19E-47TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDR354W9.471.45E-21TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YML057C-A_d7.901.40E-15YML057C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W
YLR087C7.416.48E-14CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YKL126W6.961.72E-12YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YIR005W6.651.42E-11IST3Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing
YKR019C6.341.13E-10IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YDL020C6.144.11E-10RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YCR044C6.144.21E-10PER1Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1
YNL148C6.066.80E-10ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YKL176C5.921.58E-9LST4Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
YDR137W5.715.74E-9RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YDR008C_d5.686.73E-9YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER107C5.658.12E-9GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YOR034C-A_p5.521.66E-8YOR034C-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry

GO enrichment analysis for SGTC_1119
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3196.33E-140SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0422535TSC3-RPN4
0.2942.63E-118SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0422535TSC3-RPN4
0.2807.42E-107SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0853659TSC3-RPN4
0.2773.32E-104SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.037037TSC3-RPN4
0.2711.27E-99SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0909091TSC3-RPN4
0.2691.20E-98SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.101266
0.2691.50E-98SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0735294TSC3-RPN4
0.2681.48E-97SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.0555556
0.2541.13E-87SGTC_2627acetyl isogambogic acid 100.0 μMMicrosource (Natural product library)68577890.0884956TSC3-RPN4
0.2478.74E-83SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0447761
0.2452.52E-81SGTC_10420350-0096 82.0 μMChemDiv (Drug-like library)31225150.0434783TSC3-RPN4
0.2376.91E-76SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.0649351
0.2362.83E-75SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0746269TSC3-RPN4
0.2331.99E-73SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0617284TSC3-RPN4
0.2325.52E-73SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0952381TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2560caryophyllene100 μM0.31481516667688Microsource (Natural product library)190.324484.29700
SGTC_2534helenine20.12 μM0.29032372724Microsource (Natural product library)232.31813.31102RSC complex & mRNA processing
SGTC_2673kobusone100 μM0.2586216710676Microsource (Natural product library)222.323282.25102
SGTC_2558carylophyllene oxide100 μM0.2413796708694Microsource (Natural product library)206.323883.06301
SGTC_2623humulene100 μM0.2363646187145Microsource (Natural product library)204.351065.03500
SGTC_25383',7'-epoxycaryophyllan-6-one100 μM0.2203394330530Microsource (Natural product library)236.349862.59902
SGTC_255413-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one66.67 μM0.2031256708561Microsource (Natural product library)228.32943.65701
SGTC_1113artemisinin1.18 μM0.196972240TimTec (Natural product library)282.332181.99805heme biosynthesis & mitochondrial translocase
SGTC_2650prasterone100 μM0.1969716759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.196975881Miscellaneous288.424423.33812