ficusin

furo[3,2-g]chromen-7-one

A DNA cross-linker.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1120
Screen concentration 1.8 μM
Source TimTec (Natural product library)
PubChem CID 6199
SMILES C1=CC(=O)OC2=CC3=C(C=CO3)C=C21
Standardized SMILES O=C1Oc2cc3occc3cc2C=C1
Molecular weight 186.1635
ALogP 2.2
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.04
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6199
Download HIP data (tab-delimited text)  (excel)
Gene:CDC45(YLR103C)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:GCD1(YOR260W)|FD-Score:-3.13|P-value:8.71E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HEM2(YGL040C)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.1||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:MOB1(YIL106W)|FD-Score:4.43|P-value:4.72E-6|Clearance:0.51||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NAF1(YNL124W)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.06||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:ORC6(YHR118C)|FD-Score:3.76|P-value:8.33E-5|Clearance:0.32||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:POL30(YBR088C)|FD-Score:6.73|P-value:8.45E-12|Clearance:2.3||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:RPL17A(YKL180W)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SEC62(YPL094C)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.12||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPC19(YDR201W)|FD-Score:-3.4|P-value:3.34E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SPP41(YDR464W)|FD-Score:-3.44|P-value:2.94E-4|Clearance:0||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SUI2(YJR007W)|FD-Score:-3.52|P-value:2.15E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SWI1(YPL016W)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.03||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:YCG1(YDR325W)|FD-Score:3.92|P-value:4.38E-5|Clearance:0.16||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YGL069C(YGL069C_d)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.13||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YLR140W(YLR140W_d)|FD-Score:-3.17|P-value:7.75E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:CDC45(YLR103C)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:GCD1(YOR260W)|FD-Score:-3.13|P-value:8.71E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HEM2(YGL040C)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.1||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:MOB1(YIL106W)|FD-Score:4.43|P-value:4.72E-6|Clearance:0.51||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NAF1(YNL124W)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.06||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:ORC6(YHR118C)|FD-Score:3.76|P-value:8.33E-5|Clearance:0.32||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:POL30(YBR088C)|FD-Score:6.73|P-value:8.45E-12|Clearance:2.3||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:RPL17A(YKL180W)|FD-Score:-3.61|P-value:1.54E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SEC62(YPL094C)|FD-Score:3.12|P-value:9.04E-4|Clearance:0.12||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SPC19(YDR201W)|FD-Score:-3.4|P-value:3.34E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SPP41(YDR464W)|FD-Score:-3.44|P-value:2.94E-4|Clearance:0||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SUI2(YJR007W)|FD-Score:-3.52|P-value:2.15E-4|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SWI1(YPL016W)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.03||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:YCG1(YDR325W)|FD-Score:3.92|P-value:4.38E-5|Clearance:0.16||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YGL069C(YGL069C_d)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.13||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YLR140W(YLR140W_d)|FD-Score:-3.17|P-value:7.75E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6199
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AHT1(YHR093W_d)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ATE1(YGL017W)|FD-Score:-4.77|P-value:9.07E-7||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:BCH2(YKR027W)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BIT2(YBR270C_p)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BST1(YFL025C)|FD-Score:4.57|P-value:2.41E-6||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD21(YOR078W)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CIT1(YNR001C)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CSR2(YPR030W)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DDR2(YOL052C-A)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DFM1(YDR411C)|FD-Score:6.2|P-value:2.85E-10||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DSE2(YHR143W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ERG3(YLR056W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FRE2(YKL220C)|FD-Score:-3.78|P-value:7.74E-5||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:GEM1(YAL048C)|FD-Score:-3.78|P-value:7.91E-5||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:HSE1(YHL002W)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HTD2(YHR067W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HUR1(YGL168W)|FD-Score:-3.9|P-value:4.83E-5||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HXT10(YFL011W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IDS2(YJL146W)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IES1(YFL013C)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:KEL3(YPL263C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:KIP1(YBL063W)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:MDV1(YJL112W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MID2(YLR332W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRK1(YDL079C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL38(YKL170W)|FD-Score:4.45|P-value:4.30E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL40(YPL173W)|FD-Score:-3.96|P-value:3.81E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MYO4(YAL029C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NUP120(YKL057C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OXP1(YKL215C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PCL6(YER059W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PDR3(YBL005W)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PES4(YFR023W)|FD-Score:5.47|P-value:2.25E-8||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PEX15(YOL044W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PIN4(YBL051C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PIR3(YKL163W)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PYC1(YGL062W)|FD-Score:4.97|P-value:3.30E-7||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:QDR2(YIL121W)|FD-Score:6.21|P-value:2.72E-10||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RPL11B(YGR085C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSF1(YMR030W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:SGM1(YJR134C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SMF3(YLR034C)|FD-Score:5.27|P-value:6.67E-8||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SNX4(YJL036W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPS18(YNL204C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRO7(YPR032W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSM4(YIL030C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SSZ1(YHR064C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SUS1(YBR111W-A)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TOM5(YPR133W-A)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TRP4(YDR354W)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:URA1(YKL216W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VPS38(YLR360W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:YAT1(YAR035W)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBL029W(YBL029W_p)|FD-Score:-3.9|P-value:4.72E-5||SGD DESC:Non-essential protein of unknown function Gene:YBL096C(YBL096C_d)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Non-essential protein of unknown function Gene:YDL012C(YDL012C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication Gene:YDR246W-A(YDR246W-A_p)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR514C(YDR514C)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YER175W-A(YER175W-A_p)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR039W(YGR039W_d)|FD-Score:-3.9|P-value:4.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Gene:YGR149W(YGR149W_p)|FD-Score:-4.37|P-value:6.20E-6||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YGR210C(YGR210C_p)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function Gene:YJL119C(YJL119C_d)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL202W(YKL202W_d)|FD-Score:-4.41|P-value:5.14E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR184W(YLR184W_d)|FD-Score:5.2|P-value:9.89E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL106W(YOL106W_d)|FD-Score:-4.34|P-value:7.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR289W(YOR289W_p)|FD-Score:4.82|P-value:7.20E-7||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YOR366W(YOR366W_d)|FD-Score:4.76|P-value:9.58E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YOR385W(YOR385W_p)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL191C(YPL191C_p)|FD-Score:4.96|P-value:3.57E-7||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPL216W(YPL216W_p)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:ZTA1(YBR046C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:ADK1(YDR226W)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AHT1(YHR093W_d)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ATE1(YGL017W)|FD-Score:-4.77|P-value:9.07E-7||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:BCH2(YKR027W)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BIT2(YBR270C_p)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BST1(YFL025C)|FD-Score:4.57|P-value:2.41E-6||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD21(YOR078W)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CIT1(YNR001C)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:CSR2(YPR030W)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DDR2(YOL052C-A)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DFM1(YDR411C)|FD-Score:6.2|P-value:2.85E-10||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DSE2(YHR143W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ERG3(YLR056W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FRE2(YKL220C)|FD-Score:-3.78|P-value:7.74E-5||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:GEM1(YAL048C)|FD-Score:-3.78|P-value:7.91E-5||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:HSE1(YHL002W)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes Gene:HTD2(YHR067W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HUR1(YGL168W)|FD-Score:-3.9|P-value:4.83E-5||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HXT10(YFL011W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IDS2(YJL146W)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IES1(YFL013C)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:KEL3(YPL263C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:KIP1(YBL063W)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions Gene:MDV1(YJL112W)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MID2(YLR332W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRK1(YDL079C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL38(YKL170W)|FD-Score:4.45|P-value:4.30E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL40(YPL173W)|FD-Score:-3.96|P-value:3.81E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MYO4(YAL029C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NUP120(YKL057C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OXP1(YKL215C)|FD-Score:-3.48|P-value:2.53E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PCL6(YER059W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PDR3(YBL005W)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PES4(YFR023W)|FD-Score:5.47|P-value:2.25E-8||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PEX15(YOL044W)|FD-Score:3.97|P-value:3.64E-5||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PIN4(YBL051C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PIR3(YKL163W)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PYC1(YGL062W)|FD-Score:4.97|P-value:3.30E-7||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:QDR2(YIL121W)|FD-Score:6.21|P-value:2.72E-10||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RPL11B(YGR085C)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSF1(YMR030W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:SGM1(YJR134C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SMF3(YLR034C)|FD-Score:5.27|P-value:6.67E-8||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SNX4(YJL036W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPS18(YNL204C)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRO7(YPR032W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:SSM4(YIL030C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SSZ1(YHR064C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SUS1(YBR111W-A)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TOM5(YPR133W-A)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TRP4(YDR354W)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:URA1(YKL216W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VPS38(YLR360W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:YAT1(YAR035W)|FD-Score:4.42|P-value:4.91E-6||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBL029W(YBL029W_p)|FD-Score:-3.9|P-value:4.72E-5||SGD DESC:Non-essential protein of unknown function Gene:YBL096C(YBL096C_d)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Non-essential protein of unknown function Gene:YDL012C(YDL012C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication Gene:YDR246W-A(YDR246W-A_p)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR514C(YDR514C)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication Gene:YER175W-A(YER175W-A_p)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR039W(YGR039W_d)|FD-Score:-3.9|P-value:4.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Gene:YGR149W(YGR149W_p)|FD-Score:-4.37|P-value:6.20E-6||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YGR210C(YGR210C_p)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YIL014C-A(YIL014C-A_p)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Putative protein of unknown function Gene:YJL119C(YJL119C_d)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL202W(YKL202W_d)|FD-Score:-4.41|P-value:5.14E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR184W(YLR184W_d)|FD-Score:5.2|P-value:9.89E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL106W(YOL106W_d)|FD-Score:-4.34|P-value:7.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR289W(YOR289W_p)|FD-Score:4.82|P-value:7.20E-7||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YOR366W(YOR366W_d)|FD-Score:4.76|P-value:9.58E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YOR385W(YOR385W_p)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL191C(YPL191C_p)|FD-Score:4.96|P-value:3.57E-7||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPL216W(YPL216W_p)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:ZTA1(YBR046C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR088C6.738.45E-122.30POL30Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
YIL106W4.434.72E-60.51MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YDR325W3.924.38E-50.16YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YHR118C3.768.33E-50.33ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YGL040C3.442.90E-40.10HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YPL016W3.344.22E-40.03SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YNL124W3.314.65E-40.06NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YGL069C_d3.255.68E-40.14YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YPL094C3.129.04E-40.12SEC62Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YBR142W3.000.001330.02MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YJR067C2.990.001410.12YAE1Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YLR005W2.870.002060.01SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YJL069C2.860.002104.66E-4UTP18Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
YOL040C2.860.002100.03RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YHR196W2.830.002290.01UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR078W6.351.09E-10BUD21Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
YIL121W6.212.72E-10QDR2Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper
YDR411C6.202.85E-10DFM1Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
YFR023W5.472.25E-8PES4Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication
YLR034C5.276.67E-8SMF3Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress
YLR184W_d5.209.89E-8YLR184W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL062W4.973.30E-7PYC1Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
YPL191C_p4.963.57E-7YPL191C_pPutative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YJL146W4.904.88E-7IDS2Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
YOR289W_p4.827.20E-7YOR289W_pPutative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YOR366W_d4.769.58E-7YOR366W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
YMR030W4.701.29E-6RSF1Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
YFL025C4.572.41E-6BST1GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
YKL170W4.454.30E-6MRPL38Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
YAR035W4.424.91E-6YAT1Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated

GO enrichment analysis for SGTC_1120
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0823.46E-10SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0655738cell wall signaling
0.0731.87E-8SGTC_1125tnp00248 1.3 μMTimTec (Natural product library)242077360.0701754
0.0664.25E-7SGTC_408cantharidin 100.0 μMICCB bioactive library25450.111111cell wall signaling
0.0631.20E-6SGTC_11584092-0494 91.8 μMChemDiv (Drug-like library)11481990.0735294
0.0613.27E-6SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.0892857cell wall signaling
0.0603.94E-6SGTC_33439148379 19.0 μMChembridge (Drug-like library)19550360.0555556
0.0595.81E-6SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.0666667fatty acid desaturase (OLE1)
0.0596.73E-6SGTC_422cantharidin 155.0 μMICCB bioactive library25450.111111cell wall signaling
0.0571.07E-5SGTC_6660272-0154 136.0 μMChemDiv (Drug-like library)68703120.0757576cell wall
0.0543.31E-5SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0705882cell wall signaling
0.0534.87E-5SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.0892857
0.0535.28E-5SGTC_24885268135 45.7 μMMiscellaneous12703560.0892857
0.0525.77E-5SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.0833333cell wall signaling
0.0518.00E-5SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0178571
0.0501.17E-4SGTC_7334069-0007 56.1 μMChemDiv (Drug-like library)57174250.0634921ERAD & cell cycle

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_15258-methoxypsoralen92.5 μM0.4545454114TimTec (Pure natural product library)216.189522.18704DNA damage response
SGTC_1527coumarin137 μM0.428571323TimTec (Pure natural product library)146.142741.89902
SGTC_1114scopoletin1.73 μM0.4047625280460TimTec (Natural product library)192.168121.64114
SGTC_2528xanthyletin44.79 μM0.40476265188Microsource (Natural product library)228.243282.68703
SGTC_2544sphondin68.51 μM0.361702108104Microsource (Natural product library)216.189522.18704RNA pol III & RNase P/MRP
SGTC_2619obliquin87.91 μM0.346708593Microsource (Natural product library)244.242682.77704cell wall
SGTC_2518prenyletin46.28 μM0.3333333873459Microsource (Natural product library)246.258563.10614
SGTC_1946st07605765.1 μM0.320755953009TimTec (Natural product derivative library)307.343183.87813
SGTC_1168k815-000480.5 μM0.297872642433ChemDiv (Drug-like library)201.178181.83604DNA damage response
SGTC_2556citropten84.59 μM0.2888892775Microsource (Natural product library)206.19471.86604