fisetin

2-(3,4-dihydroxyphenyl)-3,7-dihydroxychromen-4-one

Fisetin is a natural flavonol thought to be a sirtuin-activating compound; demonstrated to alleviate aging effects in the model organisms yeast S. cerevisiae, the nematode C. elegans and the fruit fly D. melanogaster.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1124
Screen concentration 1.2 μM
Source TimTec (Natural product library)
PubChem CID 5281614
SMILES C1=CC(=C(C=C1C2=C(C(=O)C3=C(O2)C=C(C=C3)O)O)O)O
Standardized SMILES OC1=C(Oc2cc(O)ccc2C1=O)c3ccc(O)c(O)c3
Molecular weight 286.2363
ALogP 1.87
H-bond donor count 4
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 24.96
% growth inhibition (Hom. pool) 2.7


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5281614
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:4.59|P-value:2.20E-6|Clearance:0.11||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP7(YPR034W)|FD-Score:-5.74|P-value:4.68E-9|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DML1(YMR211W)|FD-Score:-3.96|P-value:3.81E-5|Clearance:0||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FCP1(YMR277W)|FD-Score:4.76|P-value:9.68E-7|Clearance:0.17||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GCD11(YER025W)|FD-Score:-3.16|P-value:7.93E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:LSM2(YBL026W)|FD-Score:9.24|P-value:1.21E-20|Clearance:1.67||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM3(YLR438C-A)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAK5(YBR142W)|FD-Score:-3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:NMD3(YHR170W)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP15(YNL110C)|FD-Score:5.75|P-value:4.50E-9|Clearance:0.88||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PDC2(YDR081C)|FD-Score:-7.14|P-value:4.71E-13|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRI1(YIR008C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.03||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RHO1(YPR165W)|FD-Score:5.76|P-value:4.29E-9|Clearance:0.01||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPC17(YJL011C)|FD-Score:4.16|P-value:1.56E-5|Clearance:0.27||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPS15(YOL040C)|FD-Score:3.89|P-value:5.02E-5|Clearance:0.29||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPT6(YGL048C)|FD-Score:4.87|P-value:5.62E-7|Clearance:0.11||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:SEC14(YMR079W)|FD-Score:-6.17|P-value:3.31E-10|Clearance:0||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SEN54(YPL083C)|FD-Score:4.48|P-value:3.75E-6|Clearance:0.31||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SPC105(YGL093W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSS1(YDR086C)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.09||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:SUI2(YJR007W)|FD-Score:6.22|P-value:2.45E-10|Clearance:0.47||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TOM40(YMR203W)|FD-Score:7.57|P-value:1.86E-14|Clearance:1.35||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UFD1(YGR048W)|FD-Score:3.12|P-value:9.16E-4|Clearance:0||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:YDL196W(YDL196W_d)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YLR230W(YLR230W_d)|FD-Score:3.39|P-value:3.50E-4|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YPP1(YGR198W)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:YTH1(YPR107C)|FD-Score:-3.15|P-value:8.24E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACT1(YFL039C)|FD-Score:4.59|P-value:2.20E-6|Clearance:0.11||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP7(YPR034W)|FD-Score:-5.74|P-value:4.68E-9|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DML1(YMR211W)|FD-Score:-3.96|P-value:3.81E-5|Clearance:0||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FCP1(YMR277W)|FD-Score:4.76|P-value:9.68E-7|Clearance:0.17||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GCD11(YER025W)|FD-Score:-3.16|P-value:7.93E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:LSM2(YBL026W)|FD-Score:9.24|P-value:1.21E-20|Clearance:1.67||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM3(YLR438C-A)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAK5(YBR142W)|FD-Score:-3.09|P-value:9.99E-4|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:NMD3(YHR170W)|FD-Score:-3.36|P-value:3.94E-4|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP15(YNL110C)|FD-Score:5.75|P-value:4.50E-9|Clearance:0.88||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PDC2(YDR081C)|FD-Score:-7.14|P-value:4.71E-13|Clearance:0||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PRI1(YIR008C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.03||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RHO1(YPR165W)|FD-Score:5.76|P-value:4.29E-9|Clearance:0.01||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPC17(YJL011C)|FD-Score:4.16|P-value:1.56E-5|Clearance:0.27||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPS15(YOL040C)|FD-Score:3.89|P-value:5.02E-5|Clearance:0.29||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPT6(YGL048C)|FD-Score:4.87|P-value:5.62E-7|Clearance:0.11||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:SEC14(YMR079W)|FD-Score:-6.17|P-value:3.31E-10|Clearance:0||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SEN54(YPL083C)|FD-Score:4.48|P-value:3.75E-6|Clearance:0.31||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SPC105(YGL093W)|FD-Score:-3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSS1(YDR086C)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.09||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:SUI2(YJR007W)|FD-Score:6.22|P-value:2.45E-10|Clearance:0.47||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TOM40(YMR203W)|FD-Score:7.57|P-value:1.86E-14|Clearance:1.35||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UFD1(YGR048W)|FD-Score:3.12|P-value:9.16E-4|Clearance:0||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:YDL196W(YDL196W_d)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YLR230W(YLR230W_d)|FD-Score:3.39|P-value:3.50E-4|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:YPP1(YGR198W)|FD-Score:-3.48|P-value:2.52E-4|Clearance:0||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:YTH1(YPR107C)|FD-Score:-3.15|P-value:8.24E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5281614
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:4.88|P-value:5.32E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARG3(YJL088W)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARG81(YML099C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ARO1(YDR127W)|FD-Score:4.57|P-value:2.50E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATP17(YDR377W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AXL1(YPR122W)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BCK1(YJL095W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BNA7(YDR428C)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BUD3(YCL014W)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CAF40(YNL288W)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CDC55(YGL190C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CLN2(YPL256C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CTK3(YML112W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DAL4(YIR028W)|FD-Score:-4.78|P-value:8.60E-7||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DMC1(YER179W)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:ERG3(YLR056W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FIT2(YOR382W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:GAT4(YIR013C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDT1(YBR187W_p)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:HNT3(YOR258W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:LAG1(YHL003C)|FD-Score:-5.83|P-value:2.74E-9||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAK3(YPR051W)|FD-Score:7.19|P-value:3.29E-13||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MAK32(YCR019W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MIG2(YGL209W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MRPL40(YPL173W)|FD-Score:-4.79|P-value:8.44E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS51(YLR203C)|FD-Score:-3.96|P-value:3.73E-5||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MST27(YGL051W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles Gene:NEM1(YHR004C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NTE1(YML059C)|FD-Score:6.3|P-value:1.49E-10||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:ORM1(YGR038W)|FD-Score:10.5|P-value:6.27E-26||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAR32(YDL173W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PAU11(YGL261C_p)|FD-Score:3.81|P-value:7.09E-5||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PEP3(YLR148W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET10(YKR046C)|FD-Score:5.82|P-value:2.86E-9||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX11(YOL147C)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PRO2(YOR323C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTP1(YDL230W)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:PUG1(YER185W)|FD-Score:-3.95|P-value:3.90E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:QCR2(YPR191W)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:RAD17(YOR368W)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RHO4(YKR055W)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RHR2(YIL053W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:4.3|P-value:8.48E-6||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM20(YOR275C)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RNH202(YDR279W)|FD-Score:5.75|P-value:4.43E-9||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPN4(YDL020C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RRG9(YNL213C)|FD-Score:-4.78|P-value:8.79E-7||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTR2(YDR066C_p)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SCS3(YGL126W)|FD-Score:4.36|P-value:6.50E-6||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SHB17(YKR043C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHO1(YER118C)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SKM1(YOL113W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLG1(YOR008C)|FD-Score:7.38|P-value:8.06E-14||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SPO14(YKR031C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SSB1(YDL229W)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:STP3(YLR375W)|FD-Score:4.54|P-value:2.81E-6||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SUV3(YPL029W)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TCA17(YEL048C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TEP1(YNL128W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:TRM13(YOL125W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:UPS3(YDR185C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VAM6(YDL077C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA13(YPR036W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS24(YKL041W)|FD-Score:5.29|P-value:6.12E-8||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS64(YDR200C)|FD-Score:5.04|P-value:2.37E-7||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS9(YML097C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:XPT1(YJR133W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YBL070C(YBL070C_d)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR074W(YBR074W_p)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Putative metalloprotease Gene:YBR241C(YBR241C_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene Gene:YCL002C(YCL002C_p)|FD-Score:6.48|P-value:4.55E-11||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDL172C(YDL172C_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER097W(YER097W_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:-4.31|P-value:8.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFR018C(YFR018C_p)|FD-Score:5.23|P-value:8.63E-8||SGD DESC:Putative protein of unknown function Gene:YGL109W(YGL109W_d)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YHR080C(YHR080C)|FD-Score:4.77|P-value:9.40E-7||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIL060W(YIL060W_p)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJL160C(YJL160C_p)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJL218W(YJL218W_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YKL033W-A(YKL033W-A_p)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKR005C(YKR005C_p)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Putative protein of unknown function Gene:YKR073C(YKR073C_d)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL059C(YLL059C_d)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR177W(YLR177W_p)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR225C(YLR225C_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YMR315W(YMR315W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOR379C(YOR379C_d)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPK1(YKL126W)|FD-Score:4.35|P-value:6.96E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL102C(YPL102C_d)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPL108W(YPL108W_p)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPR174C(YPR174C_p)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication Gene:YRM1(YOR172W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes Gene:AFT1(YGL071W)|FD-Score:4.88|P-value:5.32E-7||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARG3(YJL088W)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARG81(YML099C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ARO1(YDR127W)|FD-Score:4.57|P-value:2.50E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATP17(YDR377W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AXL1(YPR122W)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BCK1(YJL095W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BNA7(YDR428C)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BUD3(YCL014W)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CAF40(YNL288W)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CDC55(YGL190C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CLN2(YPL256C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CTK3(YML112W)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DAL4(YIR028W)|FD-Score:-4.78|P-value:8.60E-7||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DMC1(YER179W)|FD-Score:-3.12|P-value:8.96E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:ERG3(YLR056W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FIT2(YOR382W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:GAT4(YIR013C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDT1(YBR187W_p)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:HNT3(YOR258W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:LAG1(YHL003C)|FD-Score:-5.83|P-value:2.74E-9||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAK3(YPR051W)|FD-Score:7.19|P-value:3.29E-13||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MAK32(YCR019W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MIG2(YGL209W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MRPL40(YPL173W)|FD-Score:-4.79|P-value:8.44E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS51(YLR203C)|FD-Score:-3.96|P-value:3.73E-5||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:MST27(YGL051W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles Gene:NEM1(YHR004C)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NTE1(YML059C)|FD-Score:6.3|P-value:1.49E-10||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:ORM1(YGR038W)|FD-Score:10.5|P-value:6.27E-26||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PAR32(YDL173W)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PAU11(YGL261C_p)|FD-Score:3.81|P-value:7.09E-5||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PEP3(YLR148W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET10(YKR046C)|FD-Score:5.82|P-value:2.86E-9||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX11(YOL147C)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PRO2(YOR323C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTP1(YDL230W)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:PUG1(YER185W)|FD-Score:-3.95|P-value:3.90E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:QCR2(YPR191W)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:RAD17(YOR368W)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RHO4(YKR055W)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RHR2(YIL053W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:4.3|P-value:8.48E-6||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM20(YOR275C)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RNH202(YDR279W)|FD-Score:5.75|P-value:4.43E-9||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPN4(YDL020C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP1B(YDL130W)|FD-Score:4.48|P-value:3.79E-6||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RRG9(YNL213C)|FD-Score:-4.78|P-value:8.79E-7||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTR2(YDR066C_p)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SCS3(YGL126W)|FD-Score:4.36|P-value:6.50E-6||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SHB17(YKR043C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHO1(YER118C)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SKM1(YOL113W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLG1(YOR008C)|FD-Score:7.38|P-value:8.06E-14||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SPO14(YKR031C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SSB1(YDL229W)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:STP3(YLR375W)|FD-Score:4.54|P-value:2.81E-6||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SUV3(YPL029W)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TCA17(YEL048C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TEP1(YNL128W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:TRM13(YOL125W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:UPS3(YDR185C)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VAM6(YDL077C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA13(YPR036W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS24(YKL041W)|FD-Score:5.29|P-value:6.12E-8||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS64(YDR200C)|FD-Score:5.04|P-value:2.37E-7||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS9(YML097C)|FD-Score:4.11|P-value:2.01E-5||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:XPT1(YJR133W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YBL070C(YBL070C_d)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR074W(YBR074W_p)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Putative metalloprotease Gene:YBR241C(YBR241C_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene Gene:YCL002C(YCL002C_p)|FD-Score:6.48|P-value:4.55E-11||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDL172C(YDL172C_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER097W(YER097W_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL032W(YFL032W_d)|FD-Score:-4.31|P-value:8.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFR018C(YFR018C_p)|FD-Score:5.23|P-value:8.63E-8||SGD DESC:Putative protein of unknown function Gene:YGL109W(YGL109W_d)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C Gene:YHR080C(YHR080C)|FD-Score:4.77|P-value:9.40E-7||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIL060W(YIL060W_p)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJL160C(YJL160C_p)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJL218W(YJL218W_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YKL033W-A(YKL033W-A_p)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YKR005C(YKR005C_p)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Putative protein of unknown function Gene:YKR073C(YKR073C_d)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL059C(YLL059C_d)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR177W(YLR177W_p)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication Gene:YLR225C(YLR225C_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR225C is not an essential gene Gene:YMR315W(YMR315W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOR379C(YOR379C_d)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPK1(YKL126W)|FD-Score:4.35|P-value:6.96E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL102C(YPL102C_d)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPL108W(YPL108W_p)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPR174C(YPR174C_p)|FD-Score:5.82|P-value:2.99E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication Gene:YRM1(YOR172W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL026W9.241.21E-201.67LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YMR203W7.571.86E-141.35TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YJR007W6.222.45E-100.47SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YPR165W5.764.29E-90.01RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YNL110C5.754.50E-90.88NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YGL048C4.875.62E-70.11RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YMR277W4.769.68E-70.17FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YFL039C4.592.20E-60.11ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YPL083C4.483.75E-60.32SEN54Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YJL011C4.161.56E-50.28RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YOL040C3.895.02E-50.29RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YDR086C3.601.57E-40.09SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YDL196W_d3.512.25E-40.12YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YLR230W_d3.393.50E-40.23YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YGR114C_d3.168.02E-40.04YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR038W10.506.27E-26ORM1Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control
YOR008C7.388.06E-14SLG1Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
YPR051W7.193.29E-13MAK3Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus
YCL002C_p6.484.55E-11YCL002C_pPutative protein of unknown function; YCL002C is not an essential gene
YML059C6.301.49E-10NTE1Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes
YKR046C5.822.86E-9PET10Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange
YPR174C_p5.822.99E-9YPR174C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication
YDR279W5.754.43E-9RNH202Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome
YDR066C_p5.561.35E-8RTR2_pProtein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication
YKL041W5.296.12E-8VPS24One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
YFR018C_p5.238.63E-8YFR018C_pPutative protein of unknown function
YDR200C5.042.37E-7VPS64Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance
YKR005C_p4.963.60E-7YKR005C_pPutative protein of unknown function
YGL071W4.885.32E-7AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YHR080C4.779.40E-7YHR080CProtein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1124
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1316.27E-24SGTC_31619100388 49.5 μMChembridge (Drug-like library)252363680.0547945iron homeostasis
0.1071.78E-16SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.0746269iron homeostasis
0.1031.75E-15SGTC_31759106517 49.5 μMChembridge (Drug-like library)414680730.057971iron homeostasis
0.1001.73E-14SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.0461538cell wall signaling
0.0961.23E-13SGTC_2666tannic acid 100.0 μMMicrosource (Natural product library)161342670.111111iron homeostasis
0.0889.69E-12SGTC_33449148862 3.9 μMChembridge (Drug-like library)49053520.0641026
0.0857.57E-11SGTC_1658st012944 20.0 μMTimTec (Natural product derivative library)242072600.0606061iron homeostasis
0.0831.65E-10SGTC_1656st012921 11.0 μMTimTec (Natural product derivative library)67328210.0606061
0.0832.04E-10SGTC_25787,8-dihydroxyflavone 64.4 μMMicrosource (Natural product library)18800.269231
0.0823.08E-10SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.155172
0.0773.23E-9SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0606061cell wall signaling
0.0764.65E-9SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.0701754cell wall signaling
0.0766.29E-9SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.117647cell wall signaling
0.0758.21E-9SGTC_33319141060 2.0 μMChembridge (Drug-like library)49065670.0641026cell wall
0.0741.13E-8SGTC_2727riluzole 51.8 μMMiscellaneous50700.0655738mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1112tnp000691.17 μM0.717949145826TimTec (Natural product library)270.23692.11435
SGTC_1109tnp00067928.26 nM0.55814676295TimTec (Natural product library)254.23752.35624
SGTC_15823',3',6'-trihydroxyflavone74 μM0.55814688803TimTec (Pure natural product library)270.23692.11435
SGTC_1562morin hydrate59.1 μM0.52272718542136TimTec (Pure natural product library)338.266261.21679
SGTC_1115galangin695.68 nM0.4565225281616TimTec (Natural product library)270.23692.11435RPP1 & pyrimidine depletion
SGTC_2505galangin7.89 μM0.4565225281616Microsource (Natural product library)270.23692.11435RPP1 & pyrimidine depletion
SGTC_1830st05598250.3 μM0.44186455313TimTec (Natural product derivative library)254.23752.35624
SGTC_11103-hydroxy-3'-methoxyflavone1.24 μM0.44676296TimTec (Natural product library)268.264082.58214iron homeostasis
SGTC_15494'-methoxyflavonol74.6 μM0.437597141TimTec (Pure natural product library)268.264082.58214
SGTC_1895st05843418.3 μM0.4130435393175TimTec (Natural product derivative library)314.334064.61413
SGTC_24977-hydroxyflavone100 μM0.4042555281894Microsource (Natural product library)238.23812.89413