asiatic acid

(1S,2R,4aS,6aS,6bR,8aR,9R,10R,11R,12aR,14bS)-10,11-dihydroxy-9-(hydroxymethyl)-1,2,6a,6b,9,12a-hexamethyl-2,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydro-1H-picene-4a-carboxylic acid

A kinase inhibitor which arrests cells at G2/M.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1127
Screen concentration 681.4 nM
Source TimTec (Natural product library)
PubChem CID 470603
SMILES CC1CCC2(CCC3(C(=CCC4C3(CCC5C4(CC(C(C5(C)CO)O)O)C)C)C2C1C)C)C(=O)O
Standardized SMILES CC1CCC2(CCC3(C)C(=CCC4C5(C)CC(O)C(O)C(C)(CO)C5CCC34C)C2C1C)C(=O)O
Molecular weight 488.6991
ALogP 4.44
H-bond donor count 4
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) -2.56
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 470603
Download HIP data (tab-delimited text)  (excel)
Gene:BET2(YPR176C)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:3.83|P-value:6.40E-5|Clearance:0.2||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD10(YNL062C)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.1||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:KAE1(YKR038C)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.09||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:LCD1(YDR499W)|FD-Score:-4.26|P-value:1.04E-5|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:LST8(YNL006W)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MCM10(YIL150C)|FD-Score:4.96|P-value:3.47E-7|Clearance:0.56||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:NET1(YJL076W)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.12||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP8(YOL144W)|FD-Score:5.26|P-value:7.12E-8|Clearance:0.3||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PIS1(YPR113W)|FD-Score:-4.01|P-value:3.01E-5|Clearance:0||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP1(YNL221C)|FD-Score:11.5|P-value:4.04E-31|Clearance:5.62||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRI1(YIR008C)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP11(YDL043C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.1||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:PRP31(YGR091W)|FD-Score:-3.92|P-value:4.43E-5|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RRP43(YCR035C)|FD-Score:5.92|P-value:1.62E-9|Clearance:0.66||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SEC16(YPL085W)|FD-Score:-3.44|P-value:2.86E-4|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEN54(YPL083C)|FD-Score:4.4|P-value:5.46E-6|Clearance:0.09||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SPC98(YNL126W)|FD-Score:-8.68|P-value:2.07E-18|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TRE2(YOR256C)|FD-Score:-4.9|P-value:4.69E-7|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:VTI1(YMR197C)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.24||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDR187C(YDR187C_d)|FD-Score:4.3|P-value:8.38E-6|Clearance:0.47||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR115C(YGR115C_d)|FD-Score:-4.22|P-value:1.25E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:BET2(YPR176C)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:3.83|P-value:6.40E-5|Clearance:0.2||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD10(YNL062C)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.1||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:KAE1(YKR038C)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.09||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:LCD1(YDR499W)|FD-Score:-4.26|P-value:1.04E-5|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:LST8(YNL006W)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MCM10(YIL150C)|FD-Score:4.96|P-value:3.47E-7|Clearance:0.56||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:NET1(YJL076W)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.12||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NOP8(YOL144W)|FD-Score:5.26|P-value:7.12E-8|Clearance:0.3||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PIS1(YPR113W)|FD-Score:-4.01|P-value:3.01E-5|Clearance:0||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP1(YNL221C)|FD-Score:11.5|P-value:4.04E-31|Clearance:5.62||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRI1(YIR008C)|FD-Score:-3.28|P-value:5.25E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP11(YDL043C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.1||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:PRP31(YGR091W)|FD-Score:-3.92|P-value:4.43E-5|Clearance:0||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RRP43(YCR035C)|FD-Score:5.92|P-value:1.62E-9|Clearance:0.66||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:SEC16(YPL085W)|FD-Score:-3.44|P-value:2.86E-4|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEN54(YPL083C)|FD-Score:4.4|P-value:5.46E-6|Clearance:0.09||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SPC98(YNL126W)|FD-Score:-8.68|P-value:2.07E-18|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TRE2(YOR256C)|FD-Score:-4.9|P-value:4.69E-7|Clearance:0||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:VTI1(YMR197C)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.24||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDR187C(YDR187C_d)|FD-Score:4.3|P-value:8.38E-6|Clearance:0.47||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR115C(YGR115C_d)|FD-Score:-4.22|P-value:1.25E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 470603
Download HOP data (tab-delimited text)  (excel)
Gene:AGX1(YFL030W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:AIM25(YJR100C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIM36(YMR157C)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:AKR1(YDR264C)|FD-Score:4.46|P-value:4.14E-6||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARA1(YBR149W)|FD-Score:-5.45|P-value:2.49E-8||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ATG18(YFR021W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATG27(YJL178C)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:ATS1(YAL020C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BUD30(YDL151C_d)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAT8(YMR280C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CBF1(YJR060W)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CGI121(YML036W)|FD-Score:7.73|P-value:5.21E-15||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COQ1(YBR003W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:COQ5(YML110C)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTK3(YML112W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCC1(YCL016C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DFM1(YDR411C)|FD-Score:5.6|P-value:1.08E-8||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:ECM5(YMR176W)|FD-Score:-5.13|P-value:1.45E-7||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EKI1(YDR147W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:FRE3(YOR381W)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FYV1(YDR024W_d)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GYP5(YPL249C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HCR1(YLR192C)|FD-Score:4.39|P-value:5.63E-6||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HIS3(YOR202W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:HOM6(YJR139C)|FD-Score:-3.99|P-value:3.26E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HPA2(YPR193C)|FD-Score:-4.72|P-value:1.15E-6||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:ICY1(YMR195W)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IFM1(YOL023W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRC21(YMR073C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IST3(YIR005W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:KTI12(YKL110C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LDB17(YDL146W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LEM3(YNL323W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LTV1(YKL143W)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MAK31(YCR020C-A)|FD-Score:5.31|P-value:5.36E-8||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family Gene:MID2(YLR332W)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MIG2(YGL209W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MOD5(YOR274W)|FD-Score:-5.68|P-value:6.81E-9||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRF1(YGL143C)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRPL37(YBR268W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NKP2(YLR315W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NOP16(YER002W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:OPI10(YOL032W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:PAU13(YHL046C)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PET18(YCR020C)|FD-Score:-4.27|P-value:9.96E-6||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PEX2(YJL210W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PIR3(YKL163W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:RKM4(YDR257C)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:ROM2(YLR371W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:ROX1(YPR065W)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL37B(YDR500C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTC5(YOR118W)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:SCP1(YOR367W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SCP160(YJL080C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCW11(YGL028C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SEC28(YIL076W)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SMF1(YOL122C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins Gene:SNX3(YOR357C)|FD-Score:6.62|P-value:1.82E-11||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SPC1(YJR010C-A)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:STM1(YLR150W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:THR4(YCR053W)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIP1(YBR067C)|FD-Score:4.81|P-value:7.58E-7||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TPS2(YDR074W)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRE1(YPL176C)|FD-Score:6.59|P-value:2.25E-11||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UBA4(YHR111W)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UGA2(YBR006W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:UMP1(YBR173C)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:YBR062C(YBR062C_p)|FD-Score:-3.84|P-value:6.14E-5||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YDL172C(YDL172C_d)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR018C(YDR018C_p)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR102C(YDR102C_d)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YER135C(YER135C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFR018C(YFR018C_p)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Putative protein of unknown function Gene:YGR001C(YGR001C_p)|FD-Score:-5.47|P-value:2.21E-8||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YGR066C(YGR066C_p)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Putative protein of unknown function Gene:YGR228W(YGR228W_d)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIL060W(YIL060W_p)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YLL020C(YLL020C_d)|FD-Score:-3.79|P-value:7.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR042C(YLR042C_p)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YNL013C(YNL013C_d)|FD-Score:-5.12|P-value:1.57E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function Gene:YNL200C(YNL200C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOP1(YPR028W)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YPK1(YKL126W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR117W(YPR117W_p)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function Gene:YPT35(YHR105W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ZRG8(YER033C)|FD-Score:-4.82|P-value:7.09E-7||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ZTA1(YBR046C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:AGX1(YFL030W)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Alanine:glyoxylate aminotransferase (AGT), catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; has similarity to mammalian and plant alanine:glyoxylate aminotransferases Gene:AIM25(YJR100C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIM36(YMR157C)|FD-Score:3.81|P-value:6.91E-5||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:AKR1(YDR264C)|FD-Score:4.46|P-value:4.14E-6||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARA1(YBR149W)|FD-Score:-5.45|P-value:2.49E-8||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ATG18(YFR021W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATG27(YJL178C)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:ATS1(YAL020C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BUD30(YDL151C_d)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAT8(YMR280C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CBF1(YJR060W)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CGI121(YML036W)|FD-Score:7.73|P-value:5.21E-15||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COQ1(YBR003W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:COQ5(YML110C)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTK3(YML112W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCC1(YCL016C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DFM1(YDR411C)|FD-Score:5.6|P-value:1.08E-8||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:ECM5(YMR176W)|FD-Score:-5.13|P-value:1.45E-7||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EKI1(YDR147W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:FRE3(YOR381W)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FYV1(YDR024W_d)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GYP5(YPL249C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HCR1(YLR192C)|FD-Score:4.39|P-value:5.63E-6||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HIS3(YOR202W)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p Gene:HOM6(YJR139C)|FD-Score:-3.99|P-value:3.26E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HPA2(YPR193C)|FD-Score:-4.72|P-value:1.15E-6||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:ICY1(YMR195W)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IFM1(YOL023W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRC21(YMR073C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IST3(YIR005W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:KTI12(YKL110C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LDB17(YDL146W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LEM3(YNL323W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LTV1(YKL143W)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MAK31(YCR020C-A)|FD-Score:5.31|P-value:5.36E-8||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family Gene:MID2(YLR332W)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MIG2(YGL209W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MOD5(YOR274W)|FD-Score:-5.68|P-value:6.81E-9||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRF1(YGL143C)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRPL37(YBR268W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NKP2(YLR315W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:NOP16(YER002W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:OPI10(YOL032W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:PAU13(YHL046C)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PET18(YCR020C)|FD-Score:-4.27|P-value:9.96E-6||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PEX2(YJL210W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PIR3(YKL163W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:RKM4(YDR257C)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:ROM2(YLR371W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:ROX1(YPR065W)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL37B(YDR500C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTC5(YOR118W)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:SCP1(YOR367W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SCP160(YJL080C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCW11(YGL028C)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SEC28(YIL076W)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SMF1(YOL122C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins Gene:SNX3(YOR357C)|FD-Score:6.62|P-value:1.82E-11||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SPC1(YJR010C-A)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:STM1(YLR150W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:THR4(YCR053W)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIP1(YBR067C)|FD-Score:4.81|P-value:7.58E-7||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TPS2(YDR074W)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRE1(YPL176C)|FD-Score:6.59|P-value:2.25E-11||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UBA4(YHR111W)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UGA2(YBR006W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:UMP1(YBR173C)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:YBR062C(YBR062C_p)|FD-Score:-3.84|P-value:6.14E-5||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YDL172C(YDL172C_d)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR018C(YDR018C_p)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR102C(YDR102C_d)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index Gene:YER135C(YER135C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFR018C(YFR018C_p)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Putative protein of unknown function Gene:YGR001C(YGR001C_p)|FD-Score:-5.47|P-value:2.21E-8||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YGR066C(YGR066C_p)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Putative protein of unknown function Gene:YGR228W(YGR228W_d)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIL060W(YIL060W_p)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YLL020C(YLL020C_d)|FD-Score:-3.79|P-value:7.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR042C(YLR042C_p)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YNL013C(YNL013C_d)|FD-Score:-5.12|P-value:1.57E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function Gene:YNL200C(YNL200C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOP1(YPR028W)|FD-Score:3.85|P-value:5.89E-5||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YPK1(YKL126W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR117W(YPR117W_p)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function Gene:YPT35(YHR105W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ZRG8(YER033C)|FD-Score:-4.82|P-value:7.09E-7||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ZTA1(YBR046C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL221C11.504.04E-315.62POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YCR035C5.921.62E-90.66RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YOL144W5.267.12E-80.30NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YIL150C4.963.47E-70.56MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YPL083C4.405.46E-60.09SEN54Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YDR187C_d4.308.38E-60.47YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YGL116W3.836.40E-50.20CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YDL043C3.631.42E-40.10PRP11Subunit of the SF3a splicing factor complex, required for spliceosome assembly
YNL062C3.532.05E-40.10GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YMR197C3.432.97E-40.24VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YKR038C3.197.00E-40.09KAE1Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription
YJL076W3.119.38E-40.12NET1Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
YGL201C2.990.001410.03MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
YEL032W2.960.001550.02MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YLR339C_d2.940.001630.01YLR339C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML036W7.735.21E-15CGI121Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification
YOR357C6.621.82E-11SNX3Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
YPL176C6.592.25E-11TRE1Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication
YDR411C5.601.08E-8DFM1Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
YOR118W5.591.15E-8RTC5Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity
YCR020C-A5.315.36E-8MAK31Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family
YOR381W5.191.06E-7FRE3Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
YER074W5.002.81E-7RPS24AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
YBR067C4.817.58E-7TIP1Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
YHL046C4.681.45E-6PAU13Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock
YDR074W4.661.61E-6TPS2Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress
YIL060W_p4.611.98E-6YIL060W_pPutative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene
YDR264C4.464.14E-6AKR1Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication
YGL143C4.444.41E-6MRF1Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability
YLR192C4.395.63E-6HCR1Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA

GO enrichment analysis for SGTC_1127
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1279.55E-23SGTC_1872amphotericin b 148.0 nMMicrosource (Natural product library)166676790.139344RNA pol III & RNase P/MRP
0.1159.73E-19SGTC_2411st077133 39.9 μMTimTec (Natural product derivative library)14031420.0625RNA pol III & RNase P/MRP
0.0904.69E-12SGTC_5394859-0462 2.5 μMChemDiv (Drug-like library)464994740.03RNA pol III & RNase P/MRP
0.0823.29E-10SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.0918367RNA pol III & RNase P/MRP
0.0691.01E-7SGTC_20965468907 200.0 μMChembridge (Fragment library)7597850.0681818
0.0673.22E-7SGTC_919tnp00227 403.6 nMTimTec (Natural product library)12692460.06RNA pol III & RNase P/MRP
0.0631.12E-6SGTC_14023909-7673 35.9 μMChemDiv (Drug-like library)53280140.0337079
0.0621.78E-6SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.0348837RNA pol III & RNase P/MRP
0.0612.98E-6SGTC_1545lovastatin 49.4 μMTimTec (Pure natural product library)129236120.11215
0.0604.24E-6SGTC_10164226-1401 38.0 μMChemDiv (Drug-like library)29016130.1RNA pol III & RNase P/MRP
0.0571.31E-5SGTC_1954st077032 68.9 μMTimTec (Natural product derivative library)6989070.0752688
0.0571.38E-5SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.049505
0.0543.12E-5SGTC_11981612-1005 86.7 μMChemDiv (Drug-like library)69985090.0535714
0.0543.85E-5SGTC_1934st074720 32.6 μMTimTec (Natural product derivative library)172509920.0526316
0.0527.47E-5SGTC_30199081971 49.5 μMChembridge (Drug-like library)164518030.04

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2583asiatic acid100 μM15320556Microsource (Natural product library)488.699124.43545
SGTC_2664lyso-paf C-1673.48 μM0.803036710742Microsource (Natural product library)504.698523.26556
SGTC_1108betulinic acid729.14 nM0.67164264945TimTec (Natural product library)456.700326.49223
SGTC_1557sumaresinolic acid42.3 μM0.4177229982268TimTec (Pure natural product library)472.699725.27734
SGTC_2616abietic acid100 μM0.2380956708678Microsource (Natural product library)302.4515.09312
SGTC_2564betulinic acid100 μM0.2298856708517Microsource (Natural product library)442.673746.3423
SGTC_2508celastrol40.54 μM0.215054122724Microsource (Natural product library)450.609625.47524PDR1
SGTC_1630st00520931.3 μM0.2072073367314TimTec (Natural product derivative library)639.904746.46507
SGTC_1757st04162031.84 μM0.25289815TimTec (Natural product derivative library)329.476342.42223
SGTC_1715st03216010.23 μM0.1956525289825TimTec (Natural product derivative library)371.513022.21813