0364-0001

N-(3-bicyclo[2.2.1]heptanylmethyl)-4-nitrobenzenesulfonamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_114
Screen concentration 490.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 4003655
SMILES C1CC2CC1CC2CNS(=O)(=O)C3=CC=C(C=C3)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(cc1)S(=O)(=O)NCC2CC3CCC2C3
Molecular weight 310.3687
ALogP 2.5
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.96
% growth inhibition (Hom. pool) 13.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4003655
Download HIP data (tab-delimited text)  (excel)
Gene:BRL1(YHR036W)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.01||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:HSH49(YOR319W)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.11||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:ILV3(YJR016C)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.03||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:MPP10(YJR002W)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.08||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NIC96(YFR002W)|FD-Score:3.22|P-value:6.47E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOC4(YPR144C)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.04||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PRP2(YNR011C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.04||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:QRI1(YDL103C)|FD-Score:-3.27|P-value:5.40E-4|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RBA50(YDR527W)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.4||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RPC37(YKR025W)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C37 Gene:RPS3(YNL178W)|FD-Score:4.65|P-value:1.67E-6|Clearance:0.47||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.04||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SGD1(YLR336C)|FD-Score:3.29|P-value:5.08E-4|Clearance:0.07||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SLD5(YDR489W)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.03||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SSY5(YJL156C)|FD-Score:3.21|P-value:6.58E-4|Clearance:0.07||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TAF12(YDR145W)|FD-Score:-3.13|P-value:8.67E-4|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF7(YMR227C)|FD-Score:3.29|P-value:4.93E-4|Clearance:0.01||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:THG1(YGR024C)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.03||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:UTP13(YLR222C)|FD-Score:4.18|P-value:1.46E-5|Clearance:0.07||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP15(YMR093W)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.01||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YAH1(YPL252C)|FD-Score:3.14|P-value:8.43E-4|Clearance:0.01||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YLR198C(YLR198C_d)|FD-Score:3.55|P-value:1.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:BRL1(YHR036W)|FD-Score:3.38|P-value:3.57E-4|Clearance:0.01||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:HSH49(YOR319W)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.11||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:ILV3(YJR016C)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.03||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:MPP10(YJR002W)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.08||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NIC96(YFR002W)|FD-Score:3.22|P-value:6.47E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOC4(YPR144C)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.04||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PRP2(YNR011C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.04||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:QRI1(YDL103C)|FD-Score:-3.27|P-value:5.40E-4|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RBA50(YDR527W)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.4||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RPC37(YKR025W)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C37 Gene:RPS3(YNL178W)|FD-Score:4.65|P-value:1.67E-6|Clearance:0.47||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP9(YPR137W)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.04||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SGD1(YLR336C)|FD-Score:3.29|P-value:5.08E-4|Clearance:0.07||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SLD5(YDR489W)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.03||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SSY5(YJL156C)|FD-Score:3.21|P-value:6.58E-4|Clearance:0.07||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TAF12(YDR145W)|FD-Score:-3.13|P-value:8.67E-4|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TAF7(YMR227C)|FD-Score:3.29|P-value:4.93E-4|Clearance:0.01||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:THG1(YGR024C)|FD-Score:3.43|P-value:2.96E-4|Clearance:0.03||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:UTP13(YLR222C)|FD-Score:4.18|P-value:1.46E-5|Clearance:0.07||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP15(YMR093W)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.01||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YAH1(YPL252C)|FD-Score:3.14|P-value:8.43E-4|Clearance:0.01||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YLR198C(YLR198C_d)|FD-Score:3.55|P-value:1.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4003655
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACF4(YJR083C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADE3(YGR204W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIM29(YKR074W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ASM4(YDL088C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ASN2(YGR124W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATS1(YAL020C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BUD19(YJL188C_d)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD30(YDL151C_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:-4.46|P-value:4.08E-6||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CHL1(YPL008W)|FD-Score:4.78|P-value:8.82E-7||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:CIN8(YEL061C)|FD-Score:4.57|P-value:2.45E-6||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CLD1(YGR110W)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CPR5(YDR304C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CTF3(YLR381W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:CYB5(YNL111C)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DGA1(YOR245C)|FD-Score:10.9|P-value:9.67E-28||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DIP5(YPL265W)|FD-Score:5.06|P-value:2.09E-7||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:DNM1(YLL001W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:EAF7(YNL136W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EMC5(YIL027C)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:EMC6(YLL014W)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ENB1(YOL158C)|FD-Score:4.44|P-value:4.57E-6||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:FCJ1(YKR016W)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GAD1(YMR250W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GAS1(YMR307W)|FD-Score:6.18|P-value:3.16E-10||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCS1(YDL226C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GET3(YDL100C)|FD-Score:8.13|P-value:2.06E-16||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GET4(YOR164C)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GLN3(YER040W)|FD-Score:4.9|P-value:4.77E-7||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GLY1(YEL046C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GPD2(YOL059W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:GPM3(YOL056W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GRX7(YBR014C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GSH2(YOL049W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:GSP2(YOR185C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GUD1(YDL238C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HIM1(YDR317W)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Protein of unknown function involved in DNA repair Gene:HNT3(YOR258W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IES3(YLR052W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC23(YOR044W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:LCL3(YGL085W_p)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LYS9(YNR050C)|FD-Score:4.33|P-value:7.50E-6||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MSB2(YGR014W)|FD-Score:4.27|P-value:9.57E-6||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSH5(YDL154W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MUS81(YDR386W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NEM1(YHR004C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NOP13(YNL175C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NPR3(YHL023C)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:ORM2(YLR350W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PEX29(YDR479C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:6.99|P-value:1.42E-12||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFA5(YDR459C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PFS1(YHR185C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PIN4(YBL051C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PKH3(YDR466W)|FD-Score:6.19|P-value:3.00E-10||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PRS5(YOL061W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:PYC1(YGL062W)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:QRI5(YLR204W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD61(YDR014W)|FD-Score:4.82|P-value:7.05E-7||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:REF2(YDR195W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:REV3(YPL167C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIF2(YLR453C)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIM15(YFL033C)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RPA14(YDR156W)|FD-Score:4.28|P-value:9.42E-6||SGD DESC:RNA polymerase I subunit A14 Gene:RPL40A(YIL148W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS17B(YDR447C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS24A(YER074W)|FD-Score:4.79|P-value:8.24E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRT13(YER066W_p)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSB1(YOR049C)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:SCS3(YGL126W)|FD-Score:7.62|P-value:1.22E-14||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SEC22(YLR268W)|FD-Score:4.93|P-value:4.05E-7||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SGF73(YGL066W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIT4(YDL047W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SNF4(YGL115W)|FD-Score:7.95|P-value:9.18E-16||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SPO7(YAL009W)|FD-Score:6.02|P-value:8.57E-10||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPS18(YNL204C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRC1(YML034W)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SRF1(YDL133W)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SVF1(YDR346C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SYC1(YOR179C)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:TDA3(YHR009C_p)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TGL5(YOR081C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:TRE1(YPL176C)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM10(YOL093W)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:UBP1(YDL122W)|FD-Score:6.48|P-value:4.51E-11||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VIK1(YPL253C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:YBL083C(YBL083C_d)|FD-Score:6.23|P-value:2.30E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR200W-A(YBR200W-A_p)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCL001W-A(YCL001W-A_p)|FD-Score:5.96|P-value:1.26E-9||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR087W(YCR087W_d)|FD-Score:3.79|P-value:7.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL183C(YDL183C)|FD-Score:4.29|P-value:8.83E-6||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR149C(YDR149C_d)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR199W(YDR199W_d)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR306C(YDR306C_p)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YEL028W(YEL028W_d)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YET1(YKL065C)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YET3(YDL072C)|FD-Score:16.9|P-value:3.76E-64||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YFH7(YFR007W)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGR125W(YGR125W_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YGR130C(YGR130C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YGR169C-A(YGR169C-A_p)|FD-Score:-4.62|P-value:1.92E-6||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YIH1(YCR059C)|FD-Score:4.93|P-value:4.18E-7||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJR111C(YJR111C_p)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YLL044W(YLL044W_d)|FD-Score:4.31|P-value:8.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR374C(YLR374C_d)|FD-Score:4.34|P-value:7.17E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YLR415C(YLR415C_p)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YMR155W(YMR155W_p)|FD-Score:-3.74|P-value:9.25E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR158C-A(YMR158C-A_p)|FD-Score:-3.14|P-value:8.46E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YMR310C(YMR310C_p)|FD-Score:4.3|P-value:8.39E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL205C(YNL205C_d)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL019W(YOL019W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOL163W(YOL163W_p)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YTP1(YNL237W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ACB1(YGR037C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACF4(YJR083C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADE3(YGR204W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIM29(YKR074W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ASM4(YDL088C)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ASN2(YGR124W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATS1(YAL020C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BUD19(YJL188C_d)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD30(YDL151C_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:-4.46|P-value:4.08E-6||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CHL1(YPL008W)|FD-Score:4.78|P-value:8.82E-7||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:CIN8(YEL061C)|FD-Score:4.57|P-value:2.45E-6||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CLD1(YGR110W)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CPR5(YDR304C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:CTF3(YLR381W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6 Gene:CYB5(YNL111C)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DGA1(YOR245C)|FD-Score:10.9|P-value:9.67E-28||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DIP5(YPL265W)|FD-Score:5.06|P-value:2.09E-7||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:DNM1(YLL001W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:EAF7(YNL136W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EMC5(YIL027C)|FD-Score:4.57|P-value:2.46E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:EMC6(YLL014W)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ENB1(YOL158C)|FD-Score:4.44|P-value:4.57E-6||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:FCJ1(YKR016W)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GAD1(YMR250W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GAS1(YMR307W)|FD-Score:6.18|P-value:3.16E-10||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCS1(YDL226C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GET3(YDL100C)|FD-Score:8.13|P-value:2.06E-16||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GET4(YOR164C)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20 Gene:GLN3(YER040W)|FD-Score:4.9|P-value:4.77E-7||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GLY1(YEL046C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GPD2(YOL059W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:GPM3(YOL056W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GRX7(YBR014C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GSH2(YOL049W)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:GSP2(YOR185C)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GUD1(YDL238C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HIM1(YDR317W)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Protein of unknown function involved in DNA repair Gene:HNT3(YOR258W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IES3(YLR052W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC23(YOR044W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication Gene:LCL3(YGL085W_p)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LYS9(YNR050C)|FD-Score:4.33|P-value:7.50E-6||SGD DESC:Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112 Gene:MSB2(YGR014W)|FD-Score:4.27|P-value:9.57E-6||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSH5(YDL154W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MUS81(YDR386W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NEM1(YHR004C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NOP13(YNL175C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NPR3(YHL023C)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:ORM2(YLR350W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PEX29(YDR479C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:6.99|P-value:1.42E-12||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PFA5(YDR459C)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PFS1(YHR185C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PIN4(YBL051C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PKH3(YDR466W)|FD-Score:6.19|P-value:3.00E-10||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PRS5(YOL061W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:PYC1(YGL062W)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:QRI5(YLR204W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD61(YDR014W)|FD-Score:4.82|P-value:7.05E-7||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:REF2(YDR195W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:REV3(YPL167C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RIF2(YLR453C)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIM15(YFL033C)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RPA14(YDR156W)|FD-Score:4.28|P-value:9.42E-6||SGD DESC:RNA polymerase I subunit A14 Gene:RPL40A(YIL148W)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS17B(YDR447C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS24A(YER074W)|FD-Score:4.79|P-value:8.24E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRT13(YER066W_p)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSB1(YOR049C)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:SCS3(YGL126W)|FD-Score:7.62|P-value:1.22E-14||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SEC22(YLR268W)|FD-Score:4.93|P-value:4.05E-7||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SGF73(YGL066W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIT4(YDL047W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SNF4(YGL115W)|FD-Score:7.95|P-value:9.18E-16||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SPO7(YAL009W)|FD-Score:6.02|P-value:8.57E-10||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPS18(YNL204C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRC1(YML034W)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SRF1(YDL133W)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SVF1(YDR346C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SYC1(YOR179C)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:TDA3(YHR009C_p)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TGL5(YOR081C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:TRE1(YPL176C)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRM10(YOL093W)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:UBP1(YDL122W)|FD-Score:6.48|P-value:4.51E-11||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VIK1(YPL253C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:YBL083C(YBL083C_d)|FD-Score:6.23|P-value:2.30E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR200W-A(YBR200W-A_p)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCL001W-A(YCL001W-A_p)|FD-Score:5.96|P-value:1.26E-9||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR087W(YCR087W_d)|FD-Score:3.79|P-value:7.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL183C(YDL183C)|FD-Score:4.29|P-value:8.83E-6||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR149C(YDR149C_d)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR199W(YDR199W_d)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR306C(YDR306C_p)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YEL028W(YEL028W_d)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YET1(YKL065C)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YET3(YDL072C)|FD-Score:16.9|P-value:3.76E-64||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YFH7(YFR007W)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGR125W(YGR125W_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YGR130C(YGR130C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YGR169C-A(YGR169C-A_p)|FD-Score:-4.62|P-value:1.92E-6||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YIH1(YCR059C)|FD-Score:4.93|P-value:4.18E-7||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YJR111C(YJR111C_p)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YLL044W(YLL044W_d)|FD-Score:4.31|P-value:8.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLR374C(YLR374C_d)|FD-Score:4.34|P-value:7.17E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YLR415C(YLR415C_p)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YMR155W(YMR155W_p)|FD-Score:-3.74|P-value:9.25E-5||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR158C-A(YMR158C-A_p)|FD-Score:-3.14|P-value:8.46E-4||SGD DESC:Putative protein of unknown function, may contain a lipid attachment site; YMR158C-A is not an essential gene Gene:YMR310C(YMR310C_p)|FD-Score:4.3|P-value:8.39E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL205C(YNL205C_d)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL019W(YOL019W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication Gene:YOL163W(YOL163W_p)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YTP1(YNL237W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YNL178W4.651.67E-60.47RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YLR222C4.181.46E-50.07UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDR527W4.111.96E-50.40RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YMR093W3.711.04E-40.01UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YNR011C3.701.08E-40.04PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YOR319W3.661.26E-40.11HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YLR198C_d3.551.95E-40.00YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YDR489W3.541.99E-40.03SLD5Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YPR144C3.512.25E-40.04NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YJR016C3.462.66E-40.03ILV3Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
YGR024C3.432.96E-40.03THG1tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
YKR025W3.413.25E-40.03RPC37RNA polymerase III subunit C37
YHR036W3.383.57E-40.01BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YJR002W3.383.68E-40.08MPP10Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
YMR227C3.294.93E-40.01TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL072C16.903.76E-64YET3Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
YOR245C10.909.67E-28DGA1Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles
YDL100C8.132.06E-16GET3Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
YGL115W7.959.18E-16SNF4Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
YGL126W7.621.22E-14SCS3Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol
YLR324W6.991.42E-12PEX30Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
YDL122W6.484.51E-11UBP1Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
YBL083C_d6.232.30E-10YBL083C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
YDR466W6.193.00E-10PKH3Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
YMR307W6.183.16E-10GAS1Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery
YAL009W6.028.57E-10SPO7Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
YCL001W-A_p5.961.26E-9YCL001W-A_pPutative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III
YPL176C5.161.21E-7TRE1Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication
YPL265W5.062.09E-7DIP5Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress
YML034W5.032.45E-7SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance

GO enrichment analysis for SGTC_114
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1931.12E-50SGTC_1150527-0192 112.0 μMChemDiv (Drug-like library)42339310.1875
0.1291.88E-23SGTC_7431275-0778 158.0 μMChemDiv (Drug-like library)28492570.180328excess fatty acid
0.1125.31E-18SGTC_9221866-0035 480.0 μMChemDiv (Drug-like library)31561690.0519481excess fatty acid
0.1062.55E-16SGTC_12550566-0045 221.0 μMChemDiv (Drug-like library)27884690.0714286excess fatty acid
0.1041.55E-15SGTC_1883229-0966 278.5 μMChemDiv (Drug-like library)57223700.0533333
0.0979.79E-14SGTC_1080509-0257 621.1 μMChemDiv (Drug-like library)5366240.147541
0.0912.17E-12SGTC_29759084847 53.7 μMChembridge (Drug-like library)272117240.128571
0.0849.26E-11SGTC_1161k007-0610 110.0 μMChemDiv (Drug-like library)58438500.0547945ERAD & cell cycle
0.0808.05E-10SGTC_28589025781 9.0 μMChembridge (Drug-like library)48949800.149254sphingolipid biosynthesis & PDR1
0.0791.58E-9SGTC_1563conessine 56.1 μMTimTec (Pure natural product library)4410820.0609756
0.0741.13E-8SGTC_2656pseudo-anisatin 100.0 μMMicrosource (Natural product library)67088090.0684932
0.0663.45E-7SGTC_960929-0093 37.3 μMChemDiv (Drug-like library)69915790.0519481calcium & mitochondrial duress
0.0631.25E-6SGTC_7950685-0265 78.0 μMChemDiv (Drug-like library)3027540.0895522excess fatty acid
0.0621.62E-6SGTC_5283492-0019 172.0 μMChemDiv (Drug-like library)28692620.0657895calcium & mitochondrial duress
0.0621.70E-6SGTC_29719092186 9.9 μMChembridge (Drug-like library)252364000.106667ergosterol biosynthesis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12410364-002250.8 μM0.6888894563895ChemDiv (Drug-like library)279.397743.09113ERG2
SGTC_8400245-0196390 μM0.4642863827528ChemDiv (Drug-like library)342.367522.217
SGTC_340469-068381.75 μM0.3571433690923ChemDiv (Drug-like library)337.307942.59217SWF1 & branched chain AA biosynthesis
SGTC_10024049-0206172 μM0.3508774662749ChemDiv (Drug-like library)310.3008432.90316
SGTC_571k831-010644.2 μM0.2950824783639ChemDiv (Drug-like library)339.793962.86815
SGTC_12920kpi-0043232 μM0.2857143333905ChemDiv (Drug-like library)315.363643.51814
SGTC_2837900847645.46 μM0.2835822992805Chembridge (Drug-like library)328.3841.21226
SGTC_9491165-0505146 μM0.2758626244267ChemDiv (Drug-like library)304.321082.15415
SGTC_5941487-158410.8 μM0.2727275013332ChemDiv (Drug-like library)346.445562.65416
SGTC_7150868-0130184 μM0.2647062876258ChemDiv (Drug-like library)439.441182.73627