3229-1848

3-(4-nitrophenyl)-2-sulfanylidene-1,3-thiazolidin-4-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1140
Screen concentration 3.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2849525
SMILES C1C(=O)N(C(=S)S1)C2=CC=C(C=C2)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(cc1)N2C(=O)CSC2=S
Molecular weight 254.2855
ALogP 2.62
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.25
% growth inhibition (Hom. pool) 2.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2849525
Download HIP data (tab-delimited text)  (excel)
Gene:EXO70(YJL085W)|FD-Score:4.62|P-value:1.90E-6|Clearance:0.41||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MCM7(YBR202W)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.15||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PWP2(YCR057C)|FD-Score:3.76|P-value:8.47E-5|Clearance:0.02||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RIB3(YDR487C)|FD-Score:3.95|P-value:3.97E-5|Clearance:0.01||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RNA14(YMR061W)|FD-Score:3.74|P-value:9.24E-5|Clearance:0.12||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:SEC1(YDR164C)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.09||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SPP381(YBR152W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.29||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SUP35(YDR172W)|FD-Score:3.94|P-value:4.06E-5|Clearance:0.16||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFC6(YDR362C)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.26||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:VHT1(YGR065C)|FD-Score:3.24|P-value:6.02E-4|Clearance:0.11||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YDR355C(YDR355C_d)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.02||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:EXO70(YJL085W)|FD-Score:4.62|P-value:1.90E-6|Clearance:0.41||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MCM7(YBR202W)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.15||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PWP2(YCR057C)|FD-Score:3.76|P-value:8.47E-5|Clearance:0.02||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RIB3(YDR487C)|FD-Score:3.95|P-value:3.97E-5|Clearance:0.01||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RNA14(YMR061W)|FD-Score:3.74|P-value:9.24E-5|Clearance:0.12||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:SEC1(YDR164C)|FD-Score:3.33|P-value:4.41E-4|Clearance:0.09||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SPP381(YBR152W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.29||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SUP35(YDR172W)|FD-Score:3.94|P-value:4.06E-5|Clearance:0.16||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFC6(YDR362C)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.26||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:VHT1(YGR065C)|FD-Score:3.24|P-value:6.02E-4|Clearance:0.11||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YDR355C(YDR355C_d)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.02||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2849525
Download HOP data (tab-delimited text)  (excel)
Gene:ACO2(YJL200C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:ARG7(YMR062C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATG1(YGL180W)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:BCH2(YKR027W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:CRS5(YOR031W)|FD-Score:6.01|P-value:9.18E-10||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CST26(YBR042C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:ECM3(YOR092W)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:ECM8(YBR076W)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Non-essential protein of unknown function Gene:EHT1(YBR177C)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:FRE8(YLR047C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GFD1(YMR255W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:HXT10(YFL011W)|FD-Score:4.9|P-value:4.79E-7||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IRC4(YDR540C)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:KGD1(YIL125W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:MEI4(YER044C-A)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MEP2(YNL142W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:ORM1(YGR038W)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PMR1(YGL167C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PRY3(YJL078C)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:RBD2(YPL246C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:REI1(YBR267W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RFS1(YBR052C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RXT3(YDL076C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SCM4(YGR049W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SIP3(YNL257C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SPE4(YLR146C)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SVL3(YPL032C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TGS1(YPL157W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRP2(YER090W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:VPS21(YOR089C)|FD-Score:-6.64|P-value:1.59E-11||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR013C(YBR013C_p)|FD-Score:6.98|P-value:1.52E-12||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCL001W-A(YCL001W-A_p)|FD-Score:4.86|P-value:5.77E-7||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR087C-A(YCR087C-A_p)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL026W(YDL026W_d)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR336W(YDR336W_p)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YDR415C(YDR415C_p)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Putative aminopeptidase Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YIL100W(YIL100W_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YLR312C(YLR312C_p)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function Gene:YMR194C-A(YMR194C-A_d)|FD-Score:3.9|P-value:4.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR283W(YOR283W)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPT11(YNL304W)|FD-Score:-3.78|P-value:7.86E-5||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:ACO2(YJL200C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol Gene:ARG7(YMR062C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATG1(YGL180W)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:BCH2(YKR027W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:CRS5(YOR031W)|FD-Score:6.01|P-value:9.18E-10||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:CST26(YBR042C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:ECM3(YOR092W)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:ECM8(YBR076W)|FD-Score:3.72|P-value:9.84E-5||SGD DESC:Non-essential protein of unknown function Gene:EHT1(YBR177C)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:FRE8(YLR047C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GFD1(YMR255W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:HXT10(YFL011W)|FD-Score:4.9|P-value:4.79E-7||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IRC4(YDR540C)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:KGD1(YIL125W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:MEI4(YER044C-A)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MEP2(YNL142W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:ORM1(YGR038W)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PMR1(YGL167C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PRY3(YJL078C)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:RBD2(YPL246C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:REI1(YBR267W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RFS1(YBR052C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication Gene:RPL14A(YKL006W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RXT3(YDL076C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SCM4(YGR049W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SIP3(YNL257C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SPE4(YLR146C)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SVL3(YPL032C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TGS1(YPL157W)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRP2(YER090W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:VPS21(YOR089C)|FD-Score:-6.64|P-value:1.59E-11||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR013C(YBR013C_p)|FD-Score:6.98|P-value:1.52E-12||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCL001W-A(YCL001W-A_p)|FD-Score:4.86|P-value:5.77E-7||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR087C-A(YCR087C-A_p)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL026W(YDL026W_d)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR336W(YDR336W_p)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YDR415C(YDR415C_p)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Putative aminopeptidase Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YIL100W(YIL100W_d)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YLR312C(YLR312C_p)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function Gene:YMR194C-A(YMR194C-A_d)|FD-Score:3.9|P-value:4.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR283W(YOR283W)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPT11(YNL304W)|FD-Score:-3.78|P-value:7.86E-5||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL085W4.621.90E-60.41EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YDR362C4.211.27E-50.26TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YDR487C3.953.97E-50.01RIB33,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
YDR172W3.944.06E-50.16SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YDR355C_d3.787.95E-50.02YDR355C_dDubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
YCR057C3.768.47E-50.02PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YMR061W3.749.24E-50.12RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YBR152W3.621.50E-40.29SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YDR164C3.334.41E-40.09SEC1Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
YGR065C3.246.02E-40.11VHT1High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
YBR202W3.138.71E-40.15MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YDR460W2.980.001420.00TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YDR141C2.980.001440.04DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
YHR172W2.940.001640.16SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YFL017W-A2.780.002740.05SMX2Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR013C_p6.981.52E-12YBR013C_pPutative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein
YOR031W6.019.18E-10CRS5Copper-binding metallothionein, required for wild-type copper resistance
YOR092W5.591.15E-8ECM3Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication
YDR540C5.071.97E-7IRC4Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YFL011W4.904.79E-7HXT10Putative hexose transporter, expressed at low levels and expression is repressed by glucose
YCL001W-A_p4.865.77E-7YCL001W-A_pPutative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III
YDL026W_d4.151.66E-5YDL026W_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
YKL006W4.072.34E-5RPL14ARibosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
YBR267W4.032.81E-5REI1Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
YMR194C-A_d3.904.71E-5YMR194C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR062C3.875.47E-5ARG7Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
YGR038W3.768.42E-5ORM1Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control
YBR076W3.729.84E-5ECM8Non-essential protein of unknown function
YLR047C3.671.20E-4FRE8Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p
YPL246C3.641.36E-4RBD2Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p

GO enrichment analysis for SGTC_1140
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0765.84E-9SGTC_13451488-0192 31.4 μMChemDiv (Drug-like library)6583480.0461538
0.0715.59E-8SGTC_32839109332 35.8 μMChembridge (Drug-like library)170272810.0810811DNA intercalators
0.0681.93E-7SGTC_20975376186 200.0 μMChembridge (Fragment library)7427550.0517241
0.0663.50E-7SGTC_11383761-0006 1.0 μMChemDiv (Drug-like library)120610870.046875
0.0596.81E-6SGTC_2767sibutramine 71.5 μMNIH Clinical Collection647650.0655738
0.0561.53E-5SGTC_23909097792 176.6 μMChembridge (Fragment library)44971490.0606061
0.0552.30E-5SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0655738DNA intercalators
0.0543.05E-5SGTC_30269090633 49.5 μMChembridge (Drug-like library)252368420.0869565
0.0543.12E-5SGTC_1759st043059 70.0 μMTimTec (Natural product derivative library)6694260.0615385amide catabolism
0.0543.29E-5SGTC_13511496-0003 46.5 μMChemDiv (Drug-like library)38919780.196429
0.0534.60E-5SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.047619Golgi
0.0535.30E-5SGTC_2700st077718 96.8 μMTimTec (Natural product derivative library)9049700.0666667
0.0525.77E-5SGTC_24625162487 171.8 μMMiscellaneous22599860.169492
0.0525.96E-5SGTC_12103013-0140 750.0 nMChemDiv (Drug-like library)9059730.09375
0.0526.57E-5SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.0740741DNA intercalators

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1903970-07903.72 μM0.4222222832352ChemDiv (Drug-like library)286.23960.73805
SGTC_13571503-130339.5 μM0.413043292895ChemDiv (Drug-like library)239.313962.7104
SGTC_10234482-388816 μM0.383303168ChemDiv (Drug-like library)267.324062.58205TSC3-RPN4
SGTC_246457174157.67 μM0.3090915285744Miscellaneous318.37083.67115
SGTC_7791360-0071233 μM0.3023267566ChemDiv (Drug-like library)246.2853.09614fatty acid desaturase (OLE1)
SGTC_7930488-0545106 μM0.297872292548ChemDiv (Drug-like library)237.212042.24105
SGTC_3710195-00067.36 μM0.2954553928593ChemDiv (Drug-like library)253.209682.70204
SGTC_12600700-16177.75 μM0.2916672853078ChemDiv (Drug-like library)235.235922.80914calcium & mitochondrial duress
SGTC_8750709-021025.7 μM0.2830196251364ChemDiv (Drug-like library)281.266143.07815fatty acid desaturase (OLE1)
SGTC_12480469-068758.6 μM0.2777784017482ChemDiv (Drug-like library)284.223722.88805