3253-0389

N-[(Z)-(2,4-dimethoxyphenyl)methylideneamino]thiophene-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1141
Screen concentration 52.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 5402031
SMILES COC1=CC(=C(C=C1)C=NNC(=O)C2=CC=CS2)OC
Standardized SMILES COc1ccc(C=NNC(=O)c2cccs2)c(OC)c1
Molecular weight 290.3376
ALogP 2.6
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.44
% growth inhibition (Hom. pool) 4.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5402031
Download HIP data (tab-delimited text)  (excel)
Gene:ERG27(YLR100W)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:GPI18(YBR004C)|FD-Score:4.04|P-value:2.73E-5|Clearance:0.43||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:MET4(YNL103W)|FD-Score:3.12|P-value:8.93E-4|Clearance:0.03||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MEX67(YPL169C)|FD-Score:5.79|P-value:3.56E-9|Clearance:1.43||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MTR3(YGR158C)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOP1(YDL014W)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.2||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:PRI2(YKL045W)|FD-Score:-3.36|P-value:3.96E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP38(YGR075C)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.1||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RET3(YPL010W)|FD-Score:3.09|P-value:9.92E-4|Clearance:0.14||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RIO1(YOR119C)|FD-Score:-3.38|P-value:3.58E-4|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RLP7(YNL002C)|FD-Score:3.21|P-value:6.55E-4|Clearance:0.03||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPC11(YDR045C)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:SEC24(YIL109C)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.05||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:TFG2(YGR005C)|FD-Score:5.46|P-value:2.33E-8|Clearance:1.43||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TID3(YIL144W)|FD-Score:-4.31|P-value:8.30E-6|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UTP21(YLR409C)|FD-Score:-3.51|P-value:2.21E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YRA1(YDR381W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.1||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:ERG27(YLR100W)|FD-Score:-3.29|P-value:5.09E-4|Clearance:0||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:GPI18(YBR004C)|FD-Score:4.04|P-value:2.73E-5|Clearance:0.43||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:MET4(YNL103W)|FD-Score:3.12|P-value:8.93E-4|Clearance:0.03||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MEX67(YPL169C)|FD-Score:5.79|P-value:3.56E-9|Clearance:1.43||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MTR3(YGR158C)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.01||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOP1(YDL014W)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.2||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:PRI2(YKL045W)|FD-Score:-3.36|P-value:3.96E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP38(YGR075C)|FD-Score:3.31|P-value:4.63E-4|Clearance:0.1||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RET3(YPL010W)|FD-Score:3.09|P-value:9.92E-4|Clearance:0.14||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RIO1(YOR119C)|FD-Score:-3.38|P-value:3.58E-4|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RLP7(YNL002C)|FD-Score:3.21|P-value:6.55E-4|Clearance:0.03||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPC11(YDR045C)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:SEC24(YIL109C)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.05||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:TFG2(YGR005C)|FD-Score:5.46|P-value:2.33E-8|Clearance:1.43||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TID3(YIL144W)|FD-Score:-4.31|P-value:8.30E-6|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UTP21(YLR409C)|FD-Score:-3.51|P-value:2.21E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YRA1(YDR381W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.1||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5402031
Download HOP data (tab-delimited text)  (excel)
Gene:ADA2(YDR448W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AEP3(YPL005W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM25(YJR100C)|FD-Score:4.55|P-value:2.62E-6||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALG6(YOR002W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APJ1(YNL077W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARC18(YLR370C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG23(YLR431C)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:AXL2(YIL140W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:BUD32(YGR262C)|FD-Score:-4.98|P-value:3.10E-7||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CRF1(YDR223W)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:CYS3(YAL012W)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DSF2(YBR007C_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FTR1(YER145C)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GSH1(YJL101C)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HSP31(YDR533C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:LAP3(YNL239W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LSM12(YHR121W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LST7(YGR057C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MGT1(YDL200C)|FD-Score:-3.72|P-value:9.77E-5||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MNT2(YGL257C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MRPL22(YNL177C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC2(YDR205W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSM1(YGR171C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:PCI8(YIL071C)|FD-Score:3.93|P-value:4.17E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:RUP1(YOR138C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SPT3(YDR392W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT7(YBR081C)|FD-Score:5.91|P-value:1.74E-9||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SRS2(YJL092W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:STI1(YOR027W)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SYH1(YPL105C)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRK1(YJL129C)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:YBR013C(YBR013C_p)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR141C(YBR141C_p)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR016W(YCR016W_p)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR003W-A(YDR003W-A_p)|FD-Score:-5.54|P-value:1.48E-8||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR015C(YDR015C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR034W-B(YDR034W-B_p)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR131C(YDR131C_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex Gene:YDR239C(YDR239C)|FD-Score:-3.77|P-value:8.23E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YFL012W(YFL012W_p)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL088W(YGL088W_d)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR042W(YGR042W_p)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YOL019W-A(YOL019W-A_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR084W(YPR084W_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function Gene:ADA2(YDR448W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AEP3(YPL005W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM25(YJR100C)|FD-Score:4.55|P-value:2.62E-6||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ALG6(YOR002W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APJ1(YNL077W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ARC18(YLR370C)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG23(YLR431C)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:AXL2(YIL140W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:BUD32(YGR262C)|FD-Score:-4.98|P-value:3.10E-7||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CRF1(YDR223W)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:CYS3(YAL012W)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DSF2(YBR007C_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FTR1(YER145C)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GSH1(YJL101C)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HSP31(YDR533C)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:LAP3(YNL239W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LSM12(YHR121W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LST7(YGR057C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MGT1(YDL200C)|FD-Score:-3.72|P-value:9.77E-5||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MNT2(YGL257C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MRPL22(YNL177C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC2(YDR205W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSM1(YGR171C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:PCI8(YIL071C)|FD-Score:3.93|P-value:4.17E-5||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:RUP1(YOR138C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SPT3(YDR392W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT7(YBR081C)|FD-Score:5.91|P-value:1.74E-9||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SRS2(YJL092W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:STI1(YOR027W)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SYH1(YPL105C)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRK1(YJL129C)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:YBR013C(YBR013C_p)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR141C(YBR141C_p)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR016W(YCR016W_p)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDR003W-A(YDR003W-A_p)|FD-Score:-5.54|P-value:1.48E-8||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR015C(YDR015C_d)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YDR034W-B(YDR034W-B_p)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR131C(YDR131C_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex Gene:YDR239C(YDR239C)|FD-Score:-3.77|P-value:8.23E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YFL012W(YFL012W_p)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL088W(YGL088W_d)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGR042W(YGR042W_p)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YOL019W-A(YOL019W-A_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR084W(YPR084W_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL169C5.793.56E-91.43MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YGR005C5.462.33E-81.43TFG2TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
YBR004C4.042.73E-50.43GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YDR381W3.611.54E-40.10YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YDL014W3.512.22E-40.20NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YGR075C3.314.63E-40.10PRP38Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly
YNL002C3.216.55E-40.03RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YIL109C3.197.23E-40.05SEC24Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
YGR158C3.138.63E-40.01MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YNL103W3.128.93E-40.03MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YPL010W3.099.92E-40.14RET3Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YGR198W2.950.001590.01YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YOL127W2.940.001660.04RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YLR197W2.900.001880.00NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YNL313C2.890.001900.03EMW1Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL101C5.941.42E-9GSH1Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
YBR081C5.911.74E-9SPT7Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
YER145C5.601.07E-8FTR1High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
YJR100C4.552.62E-6AIM25Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss
YDR034W-B_p4.092.13E-5YDR034W-B_pPredicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
YLR431C4.092.19E-5ATG23Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p
YIL071C3.934.17E-5PCI8Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
YGL088W_d3.846.24E-5YGL088W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing
YKL068W-A_p3.836.38E-5YKL068W-A_pPutative protein of unknown function; identified by homology to Ashbya gossypii
YCR016W_p3.797.55E-5YCR016W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis
YOR027W3.778.30E-5STI1Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
YGR171C3.631.41E-4MSM1Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p
YPR084W_p3.542.02E-4YPR084W_pPutative protein of unknown function
YBR007C_p3.522.18E-4DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YOL019W-A_p3.502.31E-4YOL019W-A_pIdentified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching

GO enrichment analysis for SGTC_1141
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0723.37E-8SGTC_23479049783 174.8 μMChembridge (Fragment library)64679880.112676
0.0656.28E-7SGTC_12890976-0073 69.1 μMChemDiv (Drug-like library)33911360.16129
0.0631.12E-6SGTC_32929120825 59.8 μMChembridge (Drug-like library)248249000.0789474
0.0612.40E-6SGTC_29329016711 44.5 μMChembridge (Drug-like library)71173280.197183
0.0588.94E-6SGTC_8920929-0076 29.0 μMChemDiv (Drug-like library)57510380.0923077TRP & mitochondrial translation
0.0561.94E-5SGTC_20955432005 200.0 μMChembridge (Fragment library)7837340.0625
0.0552.78E-5SGTC_6911548-0686 185.0 μMChemDiv (Drug-like library)464951330.275
0.0543.20E-5SGTC_1896st058441 89.0 μMTimTec (Natural product derivative library)6887590.1
0.0535.04E-5SGTC_29819013952 39.0 μMChembridge (Drug-like library)29963440.202899
0.0518.02E-5SGTC_1074carmofur 3.8 μMNIH Clinical Collection25770.0945946exosome
0.0491.77E-4SGTC_33379144342 33.5 μMChembridge (Drug-like library)176829490.144737
0.0482.04E-4SGTC_2570licareol 100.0 μMMicrosource (Natural product library)4431580.047619
0.0482.40E-4SGTC_23489022543 33.4 μMChembridge (Fragment library)31626780.04mitochondrial processes
0.0472.96E-4SGTC_6281636-0253 16.0 μMChemDiv (Drug-like library)44561430.102941sphingolipid biosynthesis & PDR1
0.0473.26E-4SGTC_2751anetholtrithion 17.2 μMMiscellaneous21940.169492

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_11841269-1718139 μM0.68755403821ChemDiv (Drug-like library)260.311582.6214
SGTC_9781416-051287.4 μM0.4821436814003ChemDiv (Drug-like library)270.283260.69424redox potentiating
SGTC_20535265679138 μM0.461538764595Chembridge (Fragment library)233.286242.55113
SGTC_1280906-298113.37 μM0.4406786740226ChemDiv (Drug-like library)296.343682.0124
SGTC_10523253-3998198 μM0.3823535450317ChemDiv (Drug-like library)370.359322.71127
SGTC_2811796782232.47 μM0.3787882232244Chembridge (Drug-like library)454.3947734.14129
SGTC_3187910939449.47 μM0.37288117098090Chembridge (Drug-like library)247.312822.913Golgi
SGTC_670335-0847266 μM0.3492064552207ChemDiv (Drug-like library)375.503265.80303ubiquinone biosynthesis & proteasome
SGTC_11780988-0033191 μM0.3492065392207ChemDiv (Drug-like library)349.179323.17324
SGTC_3189911068249.47 μM0.34920617122418Chembridge (Drug-like library)273.35013.61413