3253-1408

2-(4-methoxyphenyl)-2,3-dihydro-1H-perimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1142
Screen concentration 9.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 256655
SMILES COC1=CC=C(C=C1)C2NC3=CC=CC4=C3C(=CC=C4)N2
Standardized SMILES COc1ccc(cc1)C2Nc3cccc4cccc(N2)c34
Molecular weight 276.3324
ALogP 3.94
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.11
% growth inhibition (Hom. pool) 5.96


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 256655
Download HIP data (tab-delimited text)  (excel)
Gene:FAP7(YDL166C)|FD-Score:-3.91|P-value:4.58E-5|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:RPO31(YOR116C)|FD-Score:3.86|P-value:5.72E-5|Clearance:0.52||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPS5(YJR123W)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.52||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:YBL077W(YBL077W_d)|FD-Score:3.76|P-value:8.49E-5|Clearance:0.52||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YGR073C(YGR073C_d)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.52||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:FAP7(YDL166C)|FD-Score:-3.91|P-value:4.58E-5|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:RPO31(YOR116C)|FD-Score:3.86|P-value:5.72E-5|Clearance:0.52||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPS5(YJR123W)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.52||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:YBL077W(YBL077W_d)|FD-Score:3.76|P-value:8.49E-5|Clearance:0.52||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YGR073C(YGR073C_d)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.52||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 256655
Download HOP data (tab-delimited text)  (excel)
Gene:AIM25(YJR100C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ASF1(YJL115W)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATR1(YML116W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:BNS1(YGR230W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis Gene:BTS1(YPL069C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAP2(YIL034C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHL1(YPL008W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:CLB6(YGR109C)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CTI6(YPL181W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:ECM18(YDR125C)|FD-Score:6.39|P-value:8.25E-11||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FMP30(YPL103C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:GLY1(YEL046C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HCR1(YLR192C)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:IRC6(YFR043C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LEU3(YLR451W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MCM16(YPR046W)|FD-Score:-4.17|P-value:1.52E-5||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDJ1(YFL016C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDL1(YLR188W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MDY2(YOL111C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MPH1(YIR002C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRPL16(YBL038W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTM1(YGR257C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NPC2(YDL046W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OPY2(YPR075C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:OSH3(YHR073W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:RPN4(YDL020C)|FD-Score:4.51|P-value:3.32E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT16(YNL105W_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSC2(YLR357W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RTC4(YNL254C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SAS3(YBL052C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SHO1(YER118C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SNF8(YPL002C)|FD-Score:4.91|P-value:4.46E-7||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SWF1(YDR126W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TRP2(YER090W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:YBR013C(YBR013C_p)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCK3(YER123W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDL118W(YDL118W_p)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL199C(YDL199C_p)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Putative transporter, member of the sugar porter family Gene:YDR521W(YDR521W_d)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YER034W(YER034W_p)|FD-Score:-4.31|P-value:8.19E-6||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER158C(YER158C_p)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YHR127W(YHR127W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YIL067C(YIL067C_p)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Uncharacterized protein of unknown function Gene:YKL123W(YKL123W_d)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YMR196W(YMR196W_p)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNK1(YKL067W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YOL047C(YOL047C_p)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR314W(YOR314W_d)|FD-Score:-4.23|P-value:1.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR014C(YPR014C_d)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:AIM25(YJR100C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ASF1(YJL115W)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATR1(YML116W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:BNS1(YGR230W)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Protein with some similarity to Spo12p; overexpression bypasses need for Spo12p, but not required for meiosis Gene:BTS1(YPL069C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAP2(YIL034C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHL1(YPL008W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:CLB6(YGR109C)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CTI6(YPL181W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:ECM18(YDR125C)|FD-Score:6.39|P-value:8.25E-11||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:FMP30(YPL103C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:GLY1(YEL046C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HCR1(YLR192C)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:IRC6(YFR043C)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LEU3(YLR451W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MCM16(YPR046W)|FD-Score:-4.17|P-value:1.52E-5||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDJ1(YFL016C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDL1(YLR188W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MDY2(YOL111C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MPH1(YIR002C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRPL16(YBL038W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTM1(YGR257C)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NPC2(YDL046W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:OPY2(YPR075C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:OSH3(YHR073W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:RPN4(YDL020C)|FD-Score:4.51|P-value:3.32E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT16(YNL105W_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSC2(YLR357W)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RTC4(YNL254C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SAS3(YBL052C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SHO1(YER118C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SNF8(YPL002C)|FD-Score:4.91|P-value:4.46E-7||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SWF1(YDR126W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TRP2(YER090W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:YBR013C(YBR013C_p)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCK3(YER123W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDL118W(YDL118W_p)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL199C(YDL199C_p)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Putative transporter, member of the sugar porter family Gene:YDR521W(YDR521W_d)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol Gene:YER034W(YER034W_p)|FD-Score:-4.31|P-value:8.19E-6||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YER158C(YER158C_p)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YHR127W(YHR127W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YIL067C(YIL067C_p)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Uncharacterized protein of unknown function Gene:YKL123W(YKL123W_d)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YMR196W(YMR196W_p)|FD-Score:-3.35|P-value:4.09E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNK1(YKL067W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YOL047C(YOL047C_p)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR314W(YOR314W_d)|FD-Score:-4.23|P-value:1.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR014C(YPR014C_d)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR116C3.865.72E-50.52RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YBL077W_d3.768.49E-50.52YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YGR073C_d3.571.76E-40.52YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YJR123W3.492.39E-40.52RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YNL216W2.970.001470.13RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YJL002C2.850.002220.09OST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YNL137C2.750.002940.03NAM9Mitochondrial ribosomal component of the small subunit
YMR220W2.720.003240.01ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YPL124W2.710.003320.07SPC29Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
YJL173C2.650.004050.01RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YDL004W2.640.004177.08E-4ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YJR022W2.640.004180.06LSM8Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
YLR243W_p2.580.004920.01GPN3_pPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
YGL044C2.570.005040.08RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YBL035C2.490.006420.02POL12B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR125C6.398.25E-11ECM18Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
YPL002C4.914.46E-7SNF8Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
YDL020C4.513.32E-6RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YLR357W4.171.54E-5RSC2Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance
YDL118W_p4.141.73E-5YDL118W_pDubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
YNL254C4.131.81E-5RTC4Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YKL123W_d4.102.04E-5YKL123W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4
YPR075C4.092.20E-5OPY2Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
YGR257C4.082.27E-5MTM1Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor
YPL008W4.072.31E-5CHL1Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome
YGR109C4.042.72E-5CLB6B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1
YOL047C_p3.924.49E-5YOL047C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YER158C_p3.846.12E-5YER158C_pProtein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication
YIL067C_p3.758.93E-5YIL067C_pUncharacterized protein of unknown function
YLR188W3.701.06E-4MDL1Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress

GO enrichment analysis for SGTC_1142
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1063.43E-16SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.1
0.0985.36E-14SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.0606061SWF1 & branched chain AA biosynthesis
0.0943.65E-13SGTC_712r015-0009 80.6 μMChemDiv (Drug-like library)68138720.173077
0.0912.88E-12SGTC_340469-0683 81.8 μMChemDiv (Drug-like library)36909230.0645161SWF1 & branched chain AA biosynthesis
0.0904.78E-12SGTC_1788fluconazole 20.0 μMMiscellaneous33650.0483871azole & statin
0.0881.45E-11SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.12069RNA pol III & RNase P/MRP
0.0871.87E-11SGTC_395anisomycin 6.2 μMICCB bioactive library66103090.135593
0.0864.85E-11SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0727273
0.0815.82E-10SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.155556
0.0791.02E-9SGTC_813k007-0158 173.0 μMChemDiv (Drug-like library)65239160.0833333fatty acid desaturase (OLE1)
0.0781.62E-9SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.125RNA pol III & RNase P/MRP
0.0782.09E-9SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0545455
0.0723.25E-8SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0754717heme biosynthesis & mitochondrial translocase
0.0715.40E-8SGTC_1574spiramycin 23.7 μMTimTec (Pure natural product library)64198980.0517241
0.0706.71E-8SGTC_2647atorvastatin 60.2 μMMiscellaneous608230.0588235azole & statin

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_9433253-1379105 μM0.625668464ChemDiv (Drug-like library)306.358423.92424
SGTC_9373122-000546.7 μM0.354167759535ChemDiv (Drug-like library)274.316563.51312
SGTC_20214019055173 μM0.31818244827708Chembridge (Fragment library)163.216321.26112endomembrane recycling
SGTC_2780521529871.43 μM0.3018872834131Chembridge (Drug-like library)320.341942.32915
SGTC_1970st07012235.8 μM0.3102928TimTec (Natural product derivative library)254.280563.08303TSC3-RPN4
SGTC_12720839-002127 μM0.2826095027250ChemDiv (Drug-like library)210.2282.46802
SGTC_2643anethole100 μM0.282051637563Microsource (Natural product library)148.201682.76701
SGTC_2547epiafzelechin trimethyl ether100 μM0.2592596708630Microsource (Natural product library)316.34842.9415
SGTC_1745st08149574 μM0.25454513842402TimTec (Natural product derivative library)270.279962.94814RNA processing & uracil transport
SGTC_15014491-027538.4 μM0.255104718ChemDiv (Drug-like library)315.388365.01622