3486-0183

3-methoxy-6-[5-(4-methoxyphenyl)-1,2-dihydropyrazol-3-ylidene]cyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1144
Screen concentration 12.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 6744069
SMILES COC1=CC=C(C=C1)C2=CC(=C3C=CC(=CC3=O)OC)NN2
Standardized SMILES COc1ccc(cc1)c2cc([nH]n2)c3ccc(OC)cc3O
Molecular weight 296.3205
ALogP 1.02
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.53
% growth inhibition (Hom. pool) 7.03


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6744069
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:3.73|P-value:9.40E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BRR6(YGL247W)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CIA2(YHR122W)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.16||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:CSL4(YNL232W)|FD-Score:3.91|P-value:4.54E-5|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:ERG1(YGR175C)|FD-Score:3.1|P-value:9.68E-4|Clearance:0.01||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG11(YHR007C)|FD-Score:-3.23|P-value:6.13E-4|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FRQ1(YDR373W)|FD-Score:3.14|P-value:8.35E-4|Clearance:0.04||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:RIB2(YOL066C)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.13||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RNA14(YMR061W)|FD-Score:3.16|P-value:7.79E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RNA15(YGL044C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.24||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPA43(YOR340C)|FD-Score:3.9|P-value:4.88E-5|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:SDH3(YKL141W)|FD-Score:4.51|P-value:3.20E-6|Clearance:0.6||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SNU23(YDL098C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.02||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:UTP7(YER082C)|FD-Score:3.73|P-value:9.41E-5|Clearance:0.09||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTR5(YEL035C_p)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.12||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:ARC19(YKL013C)|FD-Score:3.73|P-value:9.40E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BRR6(YGL247W)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CIA2(YHR122W)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.16||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:CSL4(YNL232W)|FD-Score:3.91|P-value:4.54E-5|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:ERG1(YGR175C)|FD-Score:3.1|P-value:9.68E-4|Clearance:0.01||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG11(YHR007C)|FD-Score:-3.23|P-value:6.13E-4|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FRQ1(YDR373W)|FD-Score:3.14|P-value:8.35E-4|Clearance:0.04||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:RIB2(YOL066C)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.13||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RNA14(YMR061W)|FD-Score:3.16|P-value:7.79E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RNA15(YGL044C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.24||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPA43(YOR340C)|FD-Score:3.9|P-value:4.88E-5|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:SDH3(YKL141W)|FD-Score:4.51|P-value:3.20E-6|Clearance:0.6||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SNU23(YDL098C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.02||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:UTP7(YER082C)|FD-Score:3.73|P-value:9.41E-5|Clearance:0.09||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTR5(YEL035C_p)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.12||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6744069
Download HOP data (tab-delimited text)  (excel)
Gene:AIM41(YOR215C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALG12(YNR030W)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ARD1(YHR013C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ATG10(YLL042C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BTS1(YPL069C)|FD-Score:7.26|P-value:1.98E-13||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCW12(YLR110C)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHK1(YBR274W)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase Gene:COG6(YNL041C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX7(YMR256C)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DBP7(YKR024C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DID4(YKL002W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:ECM14(YHR132C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ELG1(YOR144C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FBP1(YLR377C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FUI1(YBL042C)|FD-Score:5.11|P-value:1.61E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV10(YIL097W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GEP4(YHR100C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIP3(YPL137C)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:4.34|P-value:7.16E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HHF1(YBR009C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:INO2(YDR123C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:KIN2(YLR096W)|FD-Score:3.83|P-value:6.54E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LEE1(YPL054W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LSB6(YJL100W)|FD-Score:6.31|P-value:1.39E-10||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MET17(YLR303W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET31(YPL038W)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MIG3(YER028C)|FD-Score:6.64|P-value:1.52E-11||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRP21(YBL090W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL16(YBL038W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:5.32|P-value:5.10E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MSS18(YPR134W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NAS6(YGR232W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:PEP7(YDR323C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX30(YLR324W)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO88(YBR106W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PRM1(YNL279W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PSR1(YLL010C)|FD-Score:6.4|P-value:7.85E-11||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:REF2(YDR195W)|FD-Score:-18.1|P-value:2.53E-73||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:REI1(YBR267W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:REV3(YPL167C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGS2(YOR107W)|FD-Score:5.31|P-value:5.43E-8||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RRI2(YOL117W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SCP160(YJL080C)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SDP1(YIL113W)|FD-Score:4.38|P-value:5.97E-6||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SEC72(YLR292C)|FD-Score:5.01|P-value:2.65E-7||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEE1(YIL064W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SPG1(YGR236C)|FD-Score:4.76|P-value:9.60E-7||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SVS1(YPL163C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:4.91|P-value:4.54E-7||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAL1(YLR354C)|FD-Score:6.25|P-value:2.08E-10||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TDA3(YHR009C_p)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:UBP13(YBL067C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UME6(YDR207C)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VPS21(YOR089C)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YDR239C(YDR239C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGL117W(YGL117W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function Gene:YHP1(YDR451C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKL133C(YKL133C_p)|FD-Score:4.41|P-value:5.07E-6||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLR040C(YLR040C_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR358C(YLR358C_p)|FD-Score:6.43|P-value:6.24E-11||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YML089C(YML089C_d)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YNL050C(YNL050C_p)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL190W(YNL190W_p)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YOL073C(YOL073C_p)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YTA6(YPL074W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:AIM41(YOR215C)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALG12(YNR030W)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ARD1(YHR013C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ATG10(YLL042C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BTS1(YPL069C)|FD-Score:7.26|P-value:1.98E-13||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCW12(YLR110C)|FD-Score:4.53|P-value:2.97E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHK1(YBR274W)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase Gene:COG6(YNL041C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX7(YMR256C)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DBP7(YKR024C)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DID4(YKL002W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:ECM14(YHR132C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Putative metalloprotease with similarity to the zinc carboxypeptidase family, required for normal cell wall assembly Gene:ELG1(YOR144C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:FBP1(YLR377C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p Gene:FUI1(YBL042C)|FD-Score:5.11|P-value:1.61E-7||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV10(YIL097W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GEP4(YHR100C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIP3(YPL137C)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:4.34|P-value:7.16E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HHF1(YBR009C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:INO2(YDR123C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:KIN2(YLR096W)|FD-Score:3.83|P-value:6.54E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LEE1(YPL054W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LSB6(YJL100W)|FD-Score:6.31|P-value:1.39E-10||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MET17(YLR303W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET31(YPL038W)|FD-Score:-3.47|P-value:2.63E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MIG3(YER028C)|FD-Score:6.64|P-value:1.52E-11||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRP21(YBL090W)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL16(YBL038W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:5.32|P-value:5.10E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MSS18(YPR134W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:NAS6(YGR232W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 Gene:PEP7(YDR323C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEX30(YLR324W)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO88(YBR106W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PRM1(YNL279W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PSR1(YLL010C)|FD-Score:6.4|P-value:7.85E-11||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:REF2(YDR195W)|FD-Score:-18.1|P-value:2.53E-73||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:REI1(YBR267W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:REV3(YPL167C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RGS2(YOR107W)|FD-Score:5.31|P-value:5.43E-8||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RRI2(YOL117W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SCP160(YJL080C)|FD-Score:4.28|P-value:9.51E-6||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SDP1(YIL113W)|FD-Score:4.38|P-value:5.97E-6||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SEC72(YLR292C)|FD-Score:5.01|P-value:2.65E-7||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEE1(YIL064W)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SPG1(YGR236C)|FD-Score:4.76|P-value:9.60E-7||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SVS1(YPL163C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:4.91|P-value:4.54E-7||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAL1(YLR354C)|FD-Score:6.25|P-value:2.08E-10||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TDA3(YHR009C_p)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:UBP13(YBL067C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UME6(YDR207C)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VPS21(YOR089C)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YDR239C(YDR239C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGL117W(YGL117W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function Gene:YHP1(YDR451C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKL133C(YKL133C_p)|FD-Score:4.41|P-value:5.07E-6||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLR040C(YLR040C_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR358C(YLR358C_p)|FD-Score:6.43|P-value:6.24E-11||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YML089C(YML089C_d)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YNL050C(YNL050C_p)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL190W(YNL190W_p)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YOL073C(YOL073C_p)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YTA6(YPL074W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL141W4.513.20E-60.60SDH3Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
YNL232W3.914.54E-50.02CSL4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
YOR340C3.904.88E-50.00RPA43RNA polymerase I subunit A43
YHR122W3.894.98E-50.16CIA2Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B
YKL013C3.739.40E-55.03E-4ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YER082C3.739.41E-50.09UTP7Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YGL044C3.651.32E-40.24RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YOL066C3.413.26E-40.13RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YEL035C_p3.285.13E-40.12UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YMR061W3.167.79E-40.00RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YDL098C3.167.85E-40.02SNU23Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome
YDR373W3.148.35E-40.04FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YGR175C3.109.68E-40.01ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YLR314C3.090.001000.01CDC3Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YLR145W3.080.001030.04RMP1Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL069C7.261.98E-13BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YER028C6.641.52E-11MIG3Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication
YLR358C_p6.436.24E-11YLR358C_pProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W
YLL010C6.407.85E-11PSR1Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p
YJL100W6.311.39E-10LSB6Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
YLR354C6.252.08E-10TAL1Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
YBR282W5.325.10E-8MRPL27Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP
YOR107W5.315.43E-8RGS2Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
YBL042C5.111.61E-7FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YOR089C5.101.72E-7VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YLR292C5.012.65E-7SEC72Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YBR231C4.914.54E-7SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YGR236C4.769.60E-7SPG1Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YHR009C_p4.731.15E-6TDA3_pPutative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1
YLR110C4.532.97E-6CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1144
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0813.75E-10SGTC_31259127534 49.5 μMChembridge (Drug-like library)16392350.126984
0.0781.68E-9SGTC_32219128209 49.5 μMChembridge (Drug-like library)164607230.128571
0.0772.51E-9SGTC_1764st044502 93.5 μMTimTec (Natural product derivative library)84070.0526316
0.0764.57E-9SGTC_1853st056710 91.2 μMTimTec (Natural product derivative library)2691670.125amide catabolism
0.0741.31E-8SGTC_13231348-1351 11.2 μMChemDiv (Drug-like library)69896540.0506329
0.0706.67E-8SGTC_748act1.228 77.9 μMChemDiv (Drug-like library)44215050.0943396redox potentiating
0.0682.00E-7SGTC_21085365811 200.0 μMChembridge (Fragment library)7802830.0701754
0.0654.89E-7SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 54.9 μMTimTec (Pure natural product library)4428820.144928redox potentiating
0.0649.73E-7SGTC_15956-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 67.5 μMTimTec (Pure natural product library)6887880.206349
0.0631.24E-6SGTC_1166k206-0078 225.0 μMChemDiv (Drug-like library)54045950.0675676
0.0604.45E-6SGTC_11463579-1023 7.0 μMChemDiv (Drug-like library)X11460.1
0.0594.77E-6SGTC_1752st044819 83.0 μMTimTec (Natural product derivative library)4412090.0666667
0.0595.01E-6SGTC_14003771-8998 51.5 μMChemDiv (Drug-like library)6707670.0821918
0.0595.36E-6SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0983607Golgi
0.0587.73E-6SGTC_24875918373 44.0 μMMiscellaneous28762790.106667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13953486-01904.35 μM0.5714296748079ChemDiv (Drug-like library)316.353242.38224
SGTC_3333486-013140.1 μM0.5531916744690ChemDiv (Drug-like library)296.320541.31525amide catabolism
SGTC_1474466-053829.23 μM0.523816742687ChemDiv (Drug-like library)236.268581.34823ergosterol biosynthesis
SGTC_8950964-000613.7 μM0.486738897ChemDiv (Drug-like library)345.190621.51524
SGTC_2491paeonol100 μM0.35555611092Microsource (Natural product library)166.17391.31113
SGTC_2636apigenin dimethyl ether100 μM0.3333335281601Microsource (Natural product library)298.290062.86115
SGTC_1111tnp00062510.7 nM0.309091676306TimTec (Natural product library)268.264082.87814
SGTC_1860st05622874.6 μM0.309091688679TimTec (Natural product derivative library)268.264082.87814
SGTC_2493resveratrol26.09 μM0.3043486255462Microsource (Natural product library)242.269863.31523
SGTC_2522biochanin a4.94 μM0.3035715280373Microsource (Natural product library)284.263482.36625RNA pol III & RNase P/MRP