3546-0597

1,2-dimethyl-N-[(4-propan-2-ylphenyl)methyl]benzimidazol-5-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1145
Screen concentration 10.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 875334
SMILES CC1=NC2=C(N1C)C=CC(=C2)NCC3=CC=C(C=C3)C(C)C
Standardized SMILES CC(C)c1ccc(CNc2ccc3c(c2)nc(C)n3C)cc1
Molecular weight 293.406
ALogP 4.4
H-bond donor count 1
H-bond acceptor count 2
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.57
% growth inhibition (Hom. pool) 7.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 875334
Download HIP data (tab-delimited text)  (excel)
Gene:BRL1(YHR036W)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.11||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCT4(YDL143W)|FD-Score:4.79|P-value:8.43E-7|Clearance:0.67||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:EXO70(YJL085W)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.15||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GCD6(YDR211W)|FD-Score:4.34|P-value:7.08E-6|Clearance:0.67||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:RPN5(YDL147W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.01||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:TAF5(YBR198C)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.67||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TPI1(YDR050C)|FD-Score:3.34|P-value:4.26E-4|Clearance:0.1||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:UBC1(YDR177W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.02||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:BRL1(YHR036W)|FD-Score:3.44|P-value:2.86E-4|Clearance:0.11||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCT4(YDL143W)|FD-Score:4.79|P-value:8.43E-7|Clearance:0.67||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:EXO70(YJL085W)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.15||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GCD6(YDR211W)|FD-Score:4.34|P-value:7.08E-6|Clearance:0.67||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:RPN5(YDL147W)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.01||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:TAF5(YBR198C)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.67||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TPI1(YDR050C)|FD-Score:3.34|P-value:4.26E-4|Clearance:0.1||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:UBC1(YDR177W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.02||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 875334
Download HOP data (tab-delimited text)  (excel)
Gene:AIM37(YNL100W)|FD-Score:4.33|P-value:7.50E-6||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIP1(YMR092C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ARG7(YMR062C)|FD-Score:4.54|P-value:2.77E-6||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATP11(YNL315C)|FD-Score:9.14|P-value:3.15E-20||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP12(YJL180C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:ATP2(YJR121W)|FD-Score:6.22|P-value:2.53E-10||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:AZR1(YGR224W)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BUD32(YGR262C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CHS7(YHR142W)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG7(YGL005C)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSH1(YBR161W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:ECM18(YDR125C)|FD-Score:4.82|P-value:7.36E-7||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:EGD1(YPL037C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:FET4(YMR319C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GLY1(YEL046C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HHO1(YPL127C)|FD-Score:6.01|P-value:9.33E-10||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:HHT1(YBR010W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:IRS4(YKR019C)|FD-Score:-3.92|P-value:4.51E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KHA1(YJL094C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:MBA1(YBR185C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MCM16(YPR046W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDJ1(YFL016C)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDL2(YPL270W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MON1(YGL124C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MPH1(YIR002C)|FD-Score:-4.8|P-value:7.95E-7||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRPS16(YPL013C)|FD-Score:-3.37|P-value:3.83E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS17(YMR188C)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB4(YOL112W)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSC2(YDR205W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:NCE101(YJL205C)|FD-Score:4.91|P-value:4.45E-7||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NGL2(YMR285C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NTC20(YBR188C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:OGG1(YML060W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PFK27(YOL136C)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:PMP1(YCR024C-A)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PPH3(YDR075W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PRO2(YOR323C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RTR1(YER139C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SHO1(YER118C)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SKN1(YGR143W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SMA1(YPL027W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNF6(YHL025W)|FD-Score:-5.78|P-value:3.78E-9||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNT309(YPR101W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPE3(YPR069C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPS4(YOR313C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SSN8(YNL025C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STI1(YOR027W)|FD-Score:6.17|P-value:3.48E-10||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SUR4(YLR372W)|FD-Score:4.31|P-value:8.04E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TOM70(YNL121C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRX2(YGR209C)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBP7(YIL156W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:VAB2(YEL005C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:YBL053W(YBL053W_d)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBP2(YGL060W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR064W(YBR064W_d)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR137W(YBR137W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); also interacts with Sgt2p; YBR137W is not an essential gene Gene:YBR141C(YBR141C_p)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR226C(YBR226C_d)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YDR186C(YDR186C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR391C(YDR391C_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGL088W(YGL088W_d)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YIL058W(YIL058W_d)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR073C(YKR073C_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML082W(YML082W_p)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YNL010W(YNL010W_p)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YOR309C(YOR309C_d)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:AIM37(YNL100W)|FD-Score:4.33|P-value:7.50E-6||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AIP1(YMR092C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ARG7(YMR062C)|FD-Score:4.54|P-value:2.77E-6||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ATP11(YNL315C)|FD-Score:9.14|P-value:3.15E-20||SGD DESC:Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase Gene:ATP12(YJL180C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:ATP2(YJR121W)|FD-Score:6.22|P-value:2.53E-10||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:AZR1(YGR224W)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily, involved in resistance to azole drugs such as ketoconazole and fluconazole Gene:BUD32(YGR262C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CHS7(YHR142W)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG7(YGL005C)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSH1(YBR161W)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:ECM18(YDR125C)|FD-Score:4.82|P-value:7.36E-7||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:EGD1(YPL037C)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:FET4(YMR319C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GLY1(YEL046C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HHO1(YPL127C)|FD-Score:6.01|P-value:9.33E-10||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:HHT1(YBR010W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation Gene:IRS4(YKR019C)|FD-Score:-3.92|P-value:4.51E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KHA1(YJL094C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:MBA1(YBR185C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MCM16(YPR046W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDJ1(YFL016C)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDL2(YPL270W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MON1(YGL124C)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MPH1(YIR002C)|FD-Score:-4.8|P-value:7.95E-7||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:MRPS16(YPL013C)|FD-Score:-3.37|P-value:3.83E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS17(YMR188C)|FD-Score:5.1|P-value:1.71E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB4(YOL112W)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MSC2(YDR205W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:NCE101(YJL205C)|FD-Score:4.91|P-value:4.45E-7||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NGL2(YMR285C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NTC20(YBR188C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:OGG1(YML060W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:PFK27(YOL136C)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:PMP1(YCR024C-A)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PPH3(YDR075W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PRO2(YOR323C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RTR1(YER139C)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SHO1(YER118C)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SKN1(YGR143W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SMA1(YPL027W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNF6(YHL025W)|FD-Score:-5.78|P-value:3.78E-9||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SNT309(YPR101W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPE3(YPR069C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Spermidine synthase, involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells Gene:SPS4(YOR313C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SSN8(YNL025C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STI1(YOR027W)|FD-Score:6.17|P-value:3.48E-10||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SUR4(YLR372W)|FD-Score:4.31|P-value:8.04E-6||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TOM70(YNL121C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRX2(YGR209C)|FD-Score:-3.09|P-value:9.96E-4||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:UBP7(YIL156W)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:VAB2(YEL005C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:YBL053W(YBL053W_d)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBP2(YGL060W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR064W(YBR064W_d)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR137W(YBR137W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Protein of unknown function; localized to the cytoplasm; binds to Replication Protein A (RPA); also interacts with Sgt2p; YBR137W is not an essential gene Gene:YBR141C(YBR141C_p)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YBR226C(YBR226C_d)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YDR186C(YDR186C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR391C(YDR391C_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGL088W(YGL088W_d)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YIL058W(YIL058W_d)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR073C(YKR073C_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML082W(YML082W_p)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YNL010W(YNL010W_p)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YOR309C(YOR309C_d)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL143W4.798.43E-70.67CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR211W4.347.08E-60.67GCD6Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
YBR198C4.141.75E-50.67TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YDR177W3.472.59E-40.02UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YDL147W3.452.78E-40.01RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YHR036W3.442.86E-40.11BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YDR050C3.344.26E-40.10TPI1Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease
YJL085W3.246.08E-40.15EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YDR212W3.080.001030.01TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YPR104C3.080.001050.13FHL1Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
YMR290C2.940.001630.19HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
YEL035C_p2.750.002950.03UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YPL010W2.720.003240.03RET3Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YDR373W2.700.003508.38E-4FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YOR361C2.700.003510.01PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL315C9.143.15E-20ATP11Molecular chaperone, required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase
YJR121W6.222.53E-10ATP2Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YOR027W6.173.48E-10STI1Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
YPL127C6.019.33E-10HHO1Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination
YFL016C5.482.15E-8MDJ1Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
YJL094C5.161.22E-7KHA1Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
YMR188C5.101.71E-7MRPS17Mitochondrial ribosomal protein of the small subunit
YJL205C4.914.45E-7NCE101Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences
YDR125C4.827.36E-7ECM18Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
YER139C4.711.24E-6RTR1CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication
YMR062C4.542.77E-6ARG7Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
YOL136C4.425.04E-6PFK276-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
YNL100W4.337.50E-6AIM37Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
YLR372W4.318.04E-6SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YGL060W4.231.16E-5YBP2Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1145
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1831.82E-45SGTC_8800833-0487 87.2 μMChemDiv (Drug-like library)760750.0833333mitochondrial processes
0.1548.65E-33SGTC_2630antimycin a 100.0 μMMicrosource (Natural product library)57021990.08mitochondrial processes
0.1248.00E-22SGTC_20114012611 177.0 μMChembridge (Fragment library)20498870.0833333mitochondrial processes
0.1071.78E-16SGTC_21765809774 34.0 μMChembridge (Fragment library)28705390.133333mitochondrial processes
0.0939.49E-13SGTC_7441594-0068 283.0 μMChemDiv (Drug-like library)6683920.0666667amide catabolism
0.0904.36E-12SGTC_1641st009299 47.1 μMTimTec (Natural product derivative library)40460540.0972222mitochondrial processes
0.0897.56E-12SGTC_23709071495 200.0 μMChembridge (Fragment library)217911030.0847458mitochondrial processes
0.0897.94E-12SGTC_22847945075 39.2 μMChembridge (Fragment library)12454020.0675676mitochondrial processes
0.0881.60E-11SGTC_8770771-0151 33.1 μMChemDiv (Drug-like library)61665230.112676
0.0857.19E-11SGTC_21775811945 192.0 μMChembridge (Fragment library)53434750.057971
0.0822.24E-10SGTC_6074491-0986 66.8 μMChemDiv (Drug-like library)6989810.149254heme requiring
0.0759.29E-9SGTC_2748diazoxide 86.7 μMMiscellaneous30190.111111
0.0715.06E-8SGTC_1093fluphenazine 8.0 μMNIH Clinical Collection66026110.0625
0.0691.32E-7SGTC_11463579-1023 7.0 μMChemDiv (Drug-like library)X11460.0649351
0.0691.32E-7SGTC_1163k060-0048 49.9 μMChemDiv (Drug-like library)68099820.101449

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2138557559377.67 μM0.36762213Chembridge (Fragment library)212.290243.04412
SGTC_21195528445120.64 μM0.309091791935Chembridge (Fragment library)212.290243.04412
SGTC_20975376186200 μM0.277778742755Chembridge (Fragment library)198.263662.55712
SGTC_2804795424019.48 μM0.275362828705Chembridge (Drug-like library)319.357183.68514
SGTC_3105912104049.47 μM0.27419441448907Chembridge (Drug-like library)268.35353.14912
SGTC_21485546263113.57 μM0.245614792097Chembridge (Fragment library)216.2541232.76313
SGTC_2644guaiazulene100 μM0.2448983515Microsource (Natural product library)198.303424.90500
SGTC_2903592346463.88 μM0.2424242876576Chembridge (Drug-like library)310.390183.86713
SGTC_6990375-0592111 μM0.233333762578ChemDiv (Drug-like library)251.326283.71822iron homeostasis
SGTC_506n9-isopropylolomoucine153 μM0.2285716610355ICCB bioactive library326.396182.41936