3909-7961

N-cycloheptylquinazolin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1149
Screen concentration 23.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 735261
SMILES C1CCCC(CC1)NC2=NC=NC3=CC=CC=C32
Standardized SMILES C1CCCC(CC1)Nc2ncnc3ccccc23
Molecular weight 241.3315
ALogP 4.05
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.75
% growth inhibition (Hom. pool) 4.09


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 735261
Download HIP data (tab-delimited text)  (excel)
Gene:CDC19(YAL038W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.02||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:COP1(YDL145C)|FD-Score:6.99|P-value:1.37E-12|Clearance:2.29||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DPS1(YLL018C)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.12||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:DYS1(YHR068W)|FD-Score:-4.4|P-value:5.47E-6|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERG29(YMR134W_p)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:ERG8(YMR220W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.02||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MCM7(YBR202W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.2||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MED7(YOL135C)|FD-Score:-3.13|P-value:8.68E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOP56(YLR197W)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PRP19(YLL036C)|FD-Score:3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RIB3(YDR487C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.18||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RNR2(YJL026W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.11||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPN7(YPR108W)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.18||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RSC9(YML127W)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC13(YLR208W)|FD-Score:4.45|P-value:4.21E-6|Clearance:0.2||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TFC1(YBR123C)|FD-Score:-3.36|P-value:3.87E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:THG1(YGR024C)|FD-Score:4.06|P-value:2.48E-5|Clearance:0.37||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:UTP21(YLR409C)|FD-Score:4.7|P-value:1.27E-6|Clearance:0.25||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:CDC19(YAL038W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.02||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:COP1(YDL145C)|FD-Score:6.99|P-value:1.37E-12|Clearance:2.29||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DPS1(YLL018C)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.12||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:DYS1(YHR068W)|FD-Score:-4.4|P-value:5.47E-6|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:ERG29(YMR134W_p)|FD-Score:-3.26|P-value:5.66E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:ERG8(YMR220W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.02||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:MCM7(YBR202W)|FD-Score:4.25|P-value:1.06E-5|Clearance:0.2||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MED7(YOL135C)|FD-Score:-3.13|P-value:8.68E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOP56(YLR197W)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PRP19(YLL036C)|FD-Score:3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RIB3(YDR487C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.18||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RNR2(YJL026W)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.11||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPN7(YPR108W)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.18||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RSC9(YML127W)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC13(YLR208W)|FD-Score:4.45|P-value:4.21E-6|Clearance:0.2||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TFC1(YBR123C)|FD-Score:-3.36|P-value:3.87E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:THG1(YGR024C)|FD-Score:4.06|P-value:2.48E-5|Clearance:0.37||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:UTP21(YLR409C)|FD-Score:4.7|P-value:1.27E-6|Clearance:0.25||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 735261
Download HOP data (tab-delimited text)  (excel)
Gene:ADH4(YGL256W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AFT1(YGL071W)|FD-Score:5.91|P-value:1.71E-9||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHP1(YLR109W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:ALG9(YNL219C)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:APE2(YKL157W)|FD-Score:-3.91|P-value:4.59E-5||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:AQY2(YLL052C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARG7(YMR062C)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:BUD32(YGR262C)|FD-Score:-5.13|P-value:1.45E-7||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CBP4(YGR174C)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CIT1(YNR001C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX10(YPL172C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:DSE1(YER124C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FMP33(YJL161W_p)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPT2(YKR067W)|FD-Score:5.23|P-value:8.55E-8||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GRX7(YBR014C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:HSL7(YBR133C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:LIP5(YOR196C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MBP1(YDL056W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MLS1(YNL117W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MRPL40(YPL173W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTM1(YGR257C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:MUB1(YMR100W)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NEJ1(YLR265C)|FD-Score:3.85|P-value:5.79E-5||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:NTO1(YPR031W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OM45(YIL136W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:OPI3(YJR073C)|FD-Score:4.43|P-value:4.65E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORT1(YOR130C)|FD-Score:-4.33|P-value:7.58E-6||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PGM1(YKL127W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PHM8(YER037W)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PRE9(YGR135W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PRM10(YJL108C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PTC7(YHR076W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:QCR10(YHR001W-A)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:RAD1(YPL022W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD27(YKL113C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RAD59(YDL059C)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RDL1(YOR285W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIM21(YNL294C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:-3.8|P-value:7.21E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS10B(YMR230W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RTR1(YER139C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAS3(YBL052C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SCP160(YJL080C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SEC22(YLR268W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SLS1(YLR139C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNX4(YJL036W)|FD-Score:3.78|P-value:7.93E-5||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPR3(YGR059W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:STB2(YMR053C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TLG2(YOL018C)|FD-Score:6.18|P-value:3.12E-10||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOF2(YKR010C)|FD-Score:-3.83|P-value:6.47E-5||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TRM12(YML005W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRP3(YKL211C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPH2(YKL119C)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:WSC2(YNL283C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YCR016W(YCR016W_p)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR064C(YCR064C_d)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL027C(YDL027C_p)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDR391C(YDR391C_p)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YFL019C(YFL019C_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR226C(YGR226C_d)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YJL016W(YJL016W_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YKR005C(YKR005C_p)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Putative protein of unknown function Gene:YLR122C(YLR122C_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR455W(YLR455W_p)|FD-Score:8.55|P-value:5.93E-18||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YLR460C(YLR460C_p)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML009W-B(YML009W-B_d)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR221C(YMR221C_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YNR063W(YNR063W_p)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YPL114W(YPL114W_d)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPL257W(YPL257W_p)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR050C(YPR050C_d)|FD-Score:3.1|P-value:9.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR084W(YPR084W_p)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Putative protein of unknown function Gene:YPT6(YLR262C)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ADH4(YGL256W)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AFT1(YGL071W)|FD-Score:5.91|P-value:1.71E-9||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHP1(YLR109W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:ALG9(YNL219C)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:APE2(YKL157W)|FD-Score:-3.91|P-value:4.59E-5||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:AQY2(YLL052C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARG7(YMR062C)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:BUD32(YGR262C)|FD-Score:-5.13|P-value:1.45E-7||SGD DESC:Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:CBP4(YGR174C)|FD-Score:4.51|P-value:3.28E-6||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CIT1(YNR001C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COG5(YNL051W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX10(YPL172C)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:DSE1(YER124C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FMP33(YJL161W_p)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPT2(YKR067W)|FD-Score:5.23|P-value:8.55E-8||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GRX7(YBR014C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:HSL7(YBR133C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:LIP5(YOR196C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MBP1(YDL056W)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MLS1(YNL117W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MRPL40(YPL173W)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTM1(YGR257C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:MUB1(YMR100W)|FD-Score:4.38|P-value:5.84E-6||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NEJ1(YLR265C)|FD-Score:3.85|P-value:5.79E-5||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:NTO1(YPR031W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OM45(YIL136W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:OPI3(YJR073C)|FD-Score:4.43|P-value:4.65E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORT1(YOR130C)|FD-Score:-4.33|P-value:7.58E-6||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PGM1(YKL127W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PHM8(YER037W)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PRE9(YGR135W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PRM10(YJL108C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PTC7(YHR076W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:QCR10(YHR001W-A)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:RAD1(YPL022W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD27(YKL113C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RAD59(YDL059C)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p Gene:RDL1(YOR285W)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RIM21(YNL294C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RPN4(YDL020C)|FD-Score:-3.8|P-value:7.21E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS10B(YMR230W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RTR1(YER139C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAS3(YBL052C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SCP160(YJL080C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SEC22(YLR268W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SLS1(YLR139C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNX4(YJL036W)|FD-Score:3.78|P-value:7.93E-5||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPR3(YGR059W)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:STB2(YMR053C)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TLG2(YOL018C)|FD-Score:6.18|P-value:3.12E-10||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOF2(YKR010C)|FD-Score:-3.83|P-value:6.47E-5||SGD DESC:Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication Gene:TRM12(YML005W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRP3(YKL211C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPH2(YKL119C)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:WSC2(YNL283C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YCR016W(YCR016W_p)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR064C(YCR064C_d)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL027C(YDL027C_p)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene Gene:YDR391C(YDR391C_p)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YFL019C(YFL019C_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR226C(YGR226C_d)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YJL016W(YJL016W_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YKR005C(YKR005C_p)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Putative protein of unknown function Gene:YLR122C(YLR122C_d)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR455W(YLR455W_p)|FD-Score:8.55|P-value:5.93E-18||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YLR460C(YLR460C_p)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML009W-B(YML009W-B_d)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR221C(YMR221C_p)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YNR063W(YNR063W_p)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YPL114W(YPL114W_d)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPL257W(YPL257W_p)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR050C(YPR050C_d)|FD-Score:3.1|P-value:9.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR084W(YPR084W_p)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Putative protein of unknown function Gene:YPT6(YLR262C)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL145C6.991.37E-122.29COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YLR409C4.701.27E-60.25UTP21Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma
YLR208W4.454.21E-60.20SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YBR202W4.251.06E-50.20MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YGR024C4.062.48E-50.37THG1tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
YMR220W3.691.13E-40.02ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YJL026W3.661.24E-40.11RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
YLL036C3.551.92E-40.00PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YDR487C3.551.96E-40.18RIB33,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
YAL038W3.363.85E-40.02CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YPR108W3.354.08E-40.18RPN7Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
YLL018C3.167.79E-40.12DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YGR186W3.050.001160.03TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YOR148C3.020.001270.02SPP2Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction
YPR137W2.990.001380.02RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR455W_p8.555.93E-18YLR455W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress
YOL018C6.183.12E-10TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YGL071W5.911.71E-9AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YKR067W5.238.55E-8GPT2Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
YKL113C4.631.85E-6RAD275' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family
YDL059C4.542.76E-6RAD59Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
YGR174C4.513.28E-6CBP4Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex
YJR073C4.434.65E-6OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YMR062C4.424.85E-6ARG7Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
YGL005C4.415.15E-6COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YMR100W4.385.84E-6MUB1MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene
YPL172C4.251.06E-5COX10Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
YLL052C4.201.34E-5AQY2Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains
YNR063W_p4.161.61E-5YNR063W_pPutative zinc-cluster protein of unknown function
YGL256W4.121.86E-5ADH4Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency

GO enrichment analysis for SGTC_1149
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1292.71E-23SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.0847458
0.1186.77E-20SGTC_21685727979 200.0 μMChembridge (Fragment library)6952100.111111
0.1127.75E-18SGTC_29005328528 85.3 μMChembridge (Drug-like library)7953590.0819672Golgi
0.1093.53E-17SGTC_389tpck 16.7 μMMiscellaneous4396470.09375
0.1096.06E-17SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.0847458
0.1087.08E-17SGTC_3061672-3436 11.6 μMChemDiv (Drug-like library)67973780.0632911Golgi
0.0921.83E-12SGTC_30989119039 49.5 μMChembridge (Drug-like library)171615280.0746269Golgi
0.0905.32E-12SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.0833333
0.0897.55E-12SGTC_5720960-0087 17.7 μMChemDiv (Drug-like library)67963650.0722892
0.0881.55E-11SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0892857
0.0871.87E-11SGTC_13201313-0481 95.3 μMChemDiv (Drug-like library)68133650.075
0.0863.37E-11SGTC_9933909-8757 62.7 μMChemDiv (Drug-like library)42883240.236364
0.0823.15E-10SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.0833333Golgi
0.0815.34E-10SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.112676Golgi
0.0815.59E-10SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.188679Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_14023909-767335.9 μM0.4222225328014ChemDiv (Drug-like library)269.728883.98313
SGTC_5304358-171428 μM0.3653851564641ChemDiv (Drug-like library)293.362964.78914
SGTC_10410206-0046107 μM0.31372650494ChemDiv (Drug-like library)258.315621.79624
SGTC_12780874-0585172 μM0.311111766306ChemDiv (Drug-like library)234.360424.3721
SGTC_11483909-766327.7 μM0.3076924084526ChemDiv (Drug-like library)320.38833.70205
SGTC_3273913785949.47 μM0.3061222377234Chembridge (Drug-like library)266.360764.53803
SGTC_14073909-790912.3 μM0.2833334411780ChemDiv (Drug-like library)319.400244.2011460S ribosome export
SGTC_22396685329187.64 μM0.282609805680Chembridge (Fragment library)203.280182.37212
SGTC_7961234-002070.7 μM0.26785746495034ChemDiv (Drug-like library)345.32084.1902SWF1 & branched chain AA biosynthesis
SGTC_11293453-1384223 μM0.2678571084452ChemDiv (Drug-like library)358.189384.01115