4088-0362

2-cyclopentylidene-2-[4-(4-hydroxyphenyl)-1,3-thiazol-2-yl]acetonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1154
Screen concentration 150.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6618304
SMILES C1CCC(=C(C#N)C2=NC(=CS2)C3=CC=C(C=C3)O)C1
Standardized SMILES Oc1ccc(cc1)c2csc(n2)C(=C3CCCC3)C#N
Molecular weight 282.3602
ALogP 4.18
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 41.21
% growth inhibition (Hom. pool) 3.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6618304
Download HIP data (tab-delimited text)  (excel)
Gene:CDC53(YDL132W)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.06||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DML1(YMR211W)|FD-Score:3.96|P-value:3.81E-5|Clearance:0.02||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.03||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GCD2(YGR083C)|FD-Score:3.14|P-value:8.35E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-3.25|P-value:5.83E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HSP10(YOR020C)|FD-Score:3.94|P-value:4.10E-5|Clearance:0.12||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:MAS1(YLR163C)|FD-Score:3.14|P-value:8.42E-4|Clearance:0.09||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MEC1(YBR136W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.05||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MED4(YOR174W)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOP15(YNL110C)|FD-Score:10.4|P-value:1.15E-25|Clearance:4.83||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:POP5(YAL033W)|FD-Score:4.52|P-value:3.16E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE8(YML092C)|FD-Score:-3.11|P-value:9.28E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRP22(YER013W)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RBA50(YDR527W)|FD-Score:-3.1|P-value:9.56E-4|Clearance:0||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RPC82(YPR190C)|FD-Score:4.36|P-value:6.55E-6|Clearance:0.17||SGD DESC:RNA polymerase III subunit C82 Gene:RPL25(YOL127W)|FD-Score:5.57|P-value:1.25E-8|Clearance:1.06||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPO21(YDL140C)|FD-Score:-4.18|P-value:1.46E-5|Clearance:0||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:RPP1(YHR062C)|FD-Score:4.51|P-value:3.20E-6|Clearance:0.15||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS5(YJR123W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.15||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SPN1(YPR133C)|FD-Score:3.82|P-value:6.77E-5|Clearance:0.23||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPP382(YLR424W)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.03||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:TFA1(YKL028W)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFA2(YKR062W)|FD-Score:-3.32|P-value:4.52E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TFC1(YBR123C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.04||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UTP25(YIL091C)|FD-Score:4.01|P-value:3.07E-5|Clearance:0.01||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:UTR5(YEL035C_p)|FD-Score:4|P-value:3.22E-5|Clearance:0.04||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YGR114C(YGR114C_d)|FD-Score:3.23|P-value:6.13E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL086C(YJL086C_d)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:CDC53(YDL132W)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.06||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DML1(YMR211W)|FD-Score:3.96|P-value:3.81E-5|Clearance:0.02||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.03||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GCD2(YGR083C)|FD-Score:3.14|P-value:8.35E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GLC7(YER133W)|FD-Score:-3.25|P-value:5.83E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HSP10(YOR020C)|FD-Score:3.94|P-value:4.10E-5|Clearance:0.12||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:MAS1(YLR163C)|FD-Score:3.14|P-value:8.42E-4|Clearance:0.09||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MEC1(YBR136W)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.05||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MED4(YOR174W)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NOP15(YNL110C)|FD-Score:10.4|P-value:1.15E-25|Clearance:4.83||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:POP5(YAL033W)|FD-Score:4.52|P-value:3.16E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE8(YML092C)|FD-Score:-3.11|P-value:9.28E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PRP22(YER013W)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RBA50(YDR527W)|FD-Score:-3.1|P-value:9.56E-4|Clearance:0||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RPC82(YPR190C)|FD-Score:4.36|P-value:6.55E-6|Clearance:0.17||SGD DESC:RNA polymerase III subunit C82 Gene:RPL25(YOL127W)|FD-Score:5.57|P-value:1.25E-8|Clearance:1.06||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPO21(YDL140C)|FD-Score:-4.18|P-value:1.46E-5|Clearance:0||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:RPP1(YHR062C)|FD-Score:4.51|P-value:3.20E-6|Clearance:0.15||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS5(YJR123W)|FD-Score:3.45|P-value:2.77E-4|Clearance:0.15||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SPN1(YPR133C)|FD-Score:3.82|P-value:6.77E-5|Clearance:0.23||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPP382(YLR424W)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.03||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:TFA1(YKL028W)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFA2(YKR062W)|FD-Score:-3.32|P-value:4.52E-4|Clearance:0||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TFC1(YBR123C)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.04||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UTP25(YIL091C)|FD-Score:4.01|P-value:3.07E-5|Clearance:0.01||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:UTR5(YEL035C_p)|FD-Score:4|P-value:3.22E-5|Clearance:0.04||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YGR114C(YGR114C_d)|FD-Score:3.23|P-value:6.13E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL086C(YJL086C_d)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6618304
Download HOP data (tab-delimited text)  (excel)
Gene:ADE4(YMR300C)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AVT5(YBL089W)|FD-Score:5.82|P-value:2.89E-9||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BRE1(YDL074C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRE5(YNR051C)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CCS1(YMR038C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL82(YNL314W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DSS1(YMR287C)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DUF1(YOL087C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:END3(YNL084C)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FMP48(YGR052W_p)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FZO1(YBR179C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GLN3(YER040W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GLY1(YEL046C)|FD-Score:3.76|P-value:8.44E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GOR1(YNL274C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HUA1(YGR268C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IFM1(YOL023W)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRC6(YFR043C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LGE1(YPL055C)|FD-Score:4.84|P-value:6.59E-7||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:MAK32(YCR019W)|FD-Score:4.44|P-value:4.55E-6||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MEH1(YKR007W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MET8(YBR213W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MNT2(YGL257C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MRE11(YMR224C)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPL27(YBR282W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPS5(YBR251W)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSD1(YPL104W)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSF1(YPR047W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:OPI9(YLR338W_d)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PHO4(YFR034C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:POP2(YNR052C)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM10(YJL108C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRS3(YHL011C)|FD-Score:7.86|P-value:1.92E-15||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PSK2(YOL045W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:RER1(YCL001W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:RGT1(YKL038W)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RIM9(YMR063W)|FD-Score:-4.15|P-value:1.66E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROM2(YLR371W)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPE1(YJL121C)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPO41(YFL036W)|FD-Score:5.87|P-value:2.17E-9||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTF1(YGL244W)|FD-Score:4.85|P-value:6.22E-7||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAS3(YBL052C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SET5(YHR207C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SIP4(YJL089W)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SPT7(YBR081C)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSD1(YDR293C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP1(YDR463W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TSR3(YOR006C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:TUF1(YOR187W)|FD-Score:4.91|P-value:4.67E-7||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TUM1(YOR251C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:VPS74(YDR372C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:WHI3(YNL197C)|FD-Score:5.13|P-value:1.49E-7||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBP2(YGL060W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR242W(YBR242W_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCR064C(YCR064C_d)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR094W(YDR094W_d)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR222W(YDR222W_p)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR239C(YDR239C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGR093W(YGR093W_p)|FD-Score:-3.23|P-value:6.11E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR290W(YGR290W_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YKL115C(YKL115C_d)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLL044W(YLL044W_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL114W(YPL114W_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR027C(YPR027C_p)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Putative protein of unknown function Gene:YPR077C(YPR077C_d)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR150W(YPR150W_d)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C Gene:ADE4(YMR300C)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:AVT5(YBL089W)|FD-Score:5.82|P-value:2.89E-9||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BRE1(YDL074C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BRE5(YNR051C)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CCS1(YMR038C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL82(YNL314W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DSS1(YMR287C)|FD-Score:4.36|P-value:6.55E-6||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DUF1(YOL087C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:END3(YNL084C)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FMP48(YGR052W_p)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FZO1(YBR179C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GLN3(YER040W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GLY1(YEL046C)|FD-Score:3.76|P-value:8.44E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GOR1(YNL274C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HUA1(YGR268C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IFM1(YOL023W)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRC6(YFR043C)|FD-Score:-3.24|P-value:6.03E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LGE1(YPL055C)|FD-Score:4.84|P-value:6.59E-7||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:MAK32(YCR019W)|FD-Score:4.44|P-value:4.55E-6||SGD DESC:Protein necessary for structural stability of L-A double-stranded RNA-containing particles Gene:MEH1(YKR007W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MET8(YBR213W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MNT2(YGL257C)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MRE11(YMR224C)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRPL27(YBR282W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPS5(YBR251W)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSD1(YPL104W)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSF1(YPR047W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:OPI9(YLR338W_d)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PHO4(YFR034C)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:POP2(YNR052C)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM10(YJL108C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRS3(YHL011C)|FD-Score:7.86|P-value:1.92E-15||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PSK2(YOL045W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:RER1(YCL001W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:RGT1(YKL038W)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RIM9(YMR063W)|FD-Score:-4.15|P-value:1.66E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROM2(YLR371W)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPE1(YJL121C)|FD-Score:3.1|P-value:9.54E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPO41(YFL036W)|FD-Score:5.87|P-value:2.17E-9||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RTF1(YGL244W)|FD-Score:4.85|P-value:6.22E-7||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAS3(YBL052C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SET5(YHR207C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SIP4(YJL089W)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SPT7(YBR081C)|FD-Score:4.3|P-value:8.41E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSD1(YDR293C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP1(YDR463W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TSR3(YOR006C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:TUF1(YOR187W)|FD-Score:4.91|P-value:4.67E-7||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TUM1(YOR251C)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:VPS74(YDR372C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:WHI3(YNL197C)|FD-Score:5.13|P-value:1.49E-7||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBP2(YGL060W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR242W(YBR242W_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCR064C(YCR064C_d)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR094W(YDR094W_d)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR222W(YDR222W_p)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR239C(YDR239C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGR093W(YGR093W_p)|FD-Score:-3.23|P-value:6.11E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR290W(YGR290W_d)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YKL115C(YKL115C_d)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLL044W(YLL044W_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YMR086C-A(YMR086C-A_d)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL114W(YPL114W_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index Gene:YPR027C(YPR027C_p)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Putative protein of unknown function Gene:YPR077C(YPR077C_d)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR150W(YPR150W_d)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL110C10.401.15E-254.83NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YOL127W5.571.25E-81.06RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YAL033W4.523.16E-60.00POP5Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR062C4.513.20E-60.15RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPR190C4.366.55E-60.17RPC82RNA polymerase III subunit C82
YJL086C_d4.191.40E-50.18YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YIL091C4.013.07E-50.01UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YEL035C_p4.003.22E-50.04UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YMR211W3.963.81E-50.02DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YOR020C3.944.10E-50.12HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YPR133C3.826.77E-50.23SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YBR136W3.591.64E-40.05MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YHR007C3.541.97E-40.03ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YDL132W3.512.22E-40.06CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YJR123W3.452.77E-40.15RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHL011C7.861.92E-15PRS35-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
YFL036W5.872.17E-9RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YBL089W5.822.89E-9AVT5Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication
YNL197C5.131.49E-7WHI3RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication
YOR187W4.914.67E-7TUF1Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans
YGL244W4.856.22E-7RTF1Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
YPL055C4.846.59E-7LGE1Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
YOR006C4.681.43E-6TSR3Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
YJR090C4.572.42E-6GRR1F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification
YBR251W4.464.18E-6MRPS5Mitochondrial ribosomal protein of the small subunit
YCR019W4.444.55E-6MAK32Protein necessary for structural stability of L-A double-stranded RNA-containing particles
YMR287C4.366.55E-6DSS13'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
YPR077C_d4.327.88E-6YPR077C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1
YBR081C4.308.41E-6SPT7Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
YPL104W4.289.29E-6MSD1Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene

GO enrichment analysis for SGTC_1154
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1172.25E-19SGTC_1080509-0257 621.1 μMChemDiv (Drug-like library)5366240.0793651
0.1089.13E-17SGTC_1040074-0074 591.0 μMChemDiv (Drug-like library)39431240.117647
0.0986.01E-14SGTC_12400356-0016 42.1 μMChemDiv (Drug-like library)43056130.0533333mitochondrial response to ROS
0.0849.07E-11SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.0666667Golgi
0.0791.09E-9SGTC_11534055-0057 267.0 μMChemDiv (Drug-like library)8827060.0547945
0.0791.22E-9SGTC_7820083-0095 30.5 μMChemDiv (Drug-like library)35917290.0731707
0.0759.37E-9SGTC_506n9-isopropylolomoucine 153.0 μMICCB bioactive library66103550.0769231
0.0722.87E-8SGTC_24984'-Methoxychalcone 54.6 μMMicrosource (Natural product library)6418180.0625RPP1 & pyrimidine depletion
0.0723.73E-8SGTC_15427-hydroxy-3'-methoxyflavone 49.7 μMTimTec (Pure natural product library)53931530.114286
0.0691.02E-7SGTC_30749116018 49.5 μMChembridge (Drug-like library)170273030.0632911RNA processing & uracil transport
0.0691.19E-7SGTC_1037k284-1091 78.7 μMChemDiv (Drug-like library)33347110.056338
0.0663.62E-7SGTC_11463579-1023 7.0 μMChemDiv (Drug-like library)X11460.0810811
0.0654.78E-7SGTC_15324',5'-dihydroxyflavone 78.7 μMTimTec (Pure natural product library)1655210.166667Golgi
0.0655.14E-7SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.0571429
0.0656.31E-7SGTC_9463434-1334 87.4 μMChemDiv (Drug-like library)40479760.084507

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1524469-0003551.23 μM0.346154697280ChemDiv (Drug-like library)268.333584.08224
SGTC_14484255-0083152 μM0.3255815399660ChemDiv (Drug-like library)263.290622.13723ERG2
SGTC_2131246-0773967 nM0.3230776251622ChemDiv (Drug-like library)323.325963.87806
SGTC_8800833-048787.2 μM0.28571476075ChemDiv (Drug-like library)252.334183.54513mitochondrial processes
SGTC_503tyrphostin 8294 μM0.27659692970ICCB bioactive library170.167441.77313TSC3-RPN4
SGTC_23829035899200 μM0.256462679Chembridge (Fragment library)288.368041.9431460S ribosome export
SGTC_2129girgensonine121.05 μM0.245283442638Chembridge (Fragment library)216.278942.44413heme biosynthesis & mitochondrial translocase
SGTC_2176580977433.97 μM0.2452832870539Chembridge (Fragment library)240.752323.38123mitochondrial processes
SGTC_6281636-025316 μM0.228074456143ChemDiv (Drug-like library)331.230245.07213sphingolipid biosynthesis & PDR1
SGTC_12440416-001552.5 μM0.21276696201ChemDiv (Drug-like library)254.323624.31922ERG2