4088-0394

2-cyano-2-(4-phenylcyclohexylidene)ethanethioamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1155
Screen concentration 48.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 2326248
SMILES C1CC(=C(C#N)C(=S)N)CCC1C2=CC=CC=C2
Standardized SMILES NC(=S)C(=C1CCC(CC1)c2ccccc2)C#N
Molecular weight 256.3659
ALogP 3.7
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.79
% growth inhibition (Hom. pool) 2.67


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2326248
Download HIP data (tab-delimited text)  (excel)
Gene:CCT5(YJR064W)|FD-Score:3.72|P-value:9.91E-5|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.14||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC14(YFR028C)|FD-Score:4.32|P-value:7.69E-6|Clearance:0.25||SGD DESC:Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis Gene:CDC24(YAL041W)|FD-Score:4.17|P-value:1.49E-5|Clearance:0.25||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG20(YJL167W)|FD-Score:4.32|P-value:7.67E-6|Clearance:0.25||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:ERG27(YLR100W)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.06||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:FAP7(YDL166C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.17||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FHL1(YPR104C)|FD-Score:-5.08|P-value:1.91E-7|Clearance:0||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:FIP1(YJR093C)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.08||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:MTR2(YKL186C)|FD-Score:3.21|P-value:6.66E-4|Clearance:0.02||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:RIO1(YOR119C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.09||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPC53(YDL150W)|FD-Score:3.46|P-value:2.71E-4|Clearance:0.17||SGD DESC:RNA polymerase III subunit C53 Gene:RPO21(YDL140C)|FD-Score:-3.96|P-value:3.80E-5|Clearance:0||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:SPC19(YDR201W)|FD-Score:3.19|P-value:7.19E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SPP381(YBR152W)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SRB7(YDR308C)|FD-Score:-3.09|P-value:9.96E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:SUP35(YDR172W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.01||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TRM112(YNR046W)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.25||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress Gene:CCT5(YJR064W)|FD-Score:3.72|P-value:9.91E-5|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.14||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC14(YFR028C)|FD-Score:4.32|P-value:7.69E-6|Clearance:0.25||SGD DESC:Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis Gene:CDC24(YAL041W)|FD-Score:4.17|P-value:1.49E-5|Clearance:0.25||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG20(YJL167W)|FD-Score:4.32|P-value:7.67E-6|Clearance:0.25||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:ERG27(YLR100W)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.06||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:FAP7(YDL166C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.17||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FHL1(YPR104C)|FD-Score:-5.08|P-value:1.91E-7|Clearance:0||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:FIP1(YJR093C)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.08||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:MTR2(YKL186C)|FD-Score:3.21|P-value:6.66E-4|Clearance:0.02||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:RIO1(YOR119C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.09||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPC53(YDL150W)|FD-Score:3.46|P-value:2.71E-4|Clearance:0.17||SGD DESC:RNA polymerase III subunit C53 Gene:RPO21(YDL140C)|FD-Score:-3.96|P-value:3.80E-5|Clearance:0||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:SPC19(YDR201W)|FD-Score:3.19|P-value:7.19E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:SPP381(YBR152W)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:SRB7(YDR308C)|FD-Score:-3.09|P-value:9.96E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:SUP35(YDR172W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.01||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TRM112(YNR046W)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.25||SGD DESC:Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2326248
Download HOP data (tab-delimited text)  (excel)
Gene:ADA2(YDR448W)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADE3(YGR204W)|FD-Score:4.98|P-value:3.11E-7||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AGE2(YIL044C)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:ATP3(YBR039W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT5(YBL089W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BCK2(YER167W)|FD-Score:7.23|P-value:2.43E-13||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CBP4(YGR174C)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CNA1(YLR433C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:COX19(YLL018C-A)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DAL7(YIR031C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DUN1(YDL101C)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:DYN3(YMR299C)|FD-Score:5.14|P-value:1.34E-7||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ELP6(YMR312W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG4(YGL012W)|FD-Score:4.81|P-value:7.53E-7||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP1(YAR002C-A)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:ESL2(YKR096W)|FD-Score:4.41|P-value:5.18E-6||SGD DESC:Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain Gene:GLN3(YER040W)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GOT1(YMR292W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:HRD1(YOL013C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:HUA1(YGR268C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IES3(YLR052W)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IMP2'(YIL154C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LAP3(YNL239W)|FD-Score:-3.87|P-value:5.44E-5||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LOT5(YKL183W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:LRS4(YDR439W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAC1(YMR021C)|FD-Score:3.75|P-value:8.69E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET1(YKR069W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MFT1(YML062C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MNL2(YLR057W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:NAS2(YIL007C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:OAR1(YKL055C)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OCA1(YNL099C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OPI1(YHL020C)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PAU17(YLL025W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PCL8(YPL219W)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PIL1(YGR086C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PKR1(YMR123W)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPH3(YDR075W)|FD-Score:4.85|P-value:6.07E-7||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:RAD1(YPL022W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RPL16A(YIL133C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS21B(YJL136C)|FD-Score:4.86|P-value:5.92E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RSC1(YGR056W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT109(YLL002W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SAS3(YBL052C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SCP160(YJL080C)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCT1(YBL011W)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SGE1(YPR198W)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SGN1(YIR001C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SLO1(YER180C-A)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SNF3(YDL194W)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:5.7|P-value:6.07E-9||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TMA23(YMR269W)|FD-Score:4.34|P-value:7.11E-6||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TPS2(YDR074W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRM2(YKR056W)|FD-Score:3.76|P-value:8.49E-5||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:UBI4(YLL039C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBX7(YBR273C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:VBA3(YCL069W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VFA1(YER128W)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VID28(YIL017C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPS51(YKR020W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:WHI3(YNL197C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBL095W(YBL095W_p)|FD-Score:4.41|P-value:5.06E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR206W(YBR206W_d)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YDR132C(YDR132C_p)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication Gene:YDR271C(YDR271C_d)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YGR130C(YGR130C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YIL163C(YIL163C_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL097C(YKL097C_d)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKR005C(YKR005C_p)|FD-Score:5.01|P-value:2.75E-7||SGD DESC:Putative protein of unknown function Gene:YKR104W(YKR104W)|FD-Score:5.17|P-value:1.17E-7||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YPR014C(YPR014C_d)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR150W(YPR150W_d)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C Gene:YPS3(YLR121C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPS6(YIR039C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT53(YNL093W)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ADA2(YDR448W)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADE3(YGR204W)|FD-Score:4.98|P-value:3.11E-7||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AGE2(YIL044C)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:ATP3(YBR039W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT5(YBL089W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BCK2(YER167W)|FD-Score:7.23|P-value:2.43E-13||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CBP4(YGR174C)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CNA1(YLR433C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:COX19(YLL018C-A)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:DAL7(YIR031C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DUN1(YDL101C)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:DYN3(YMR299C)|FD-Score:5.14|P-value:1.34E-7||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ELP6(YMR312W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG4(YGL012W)|FD-Score:4.81|P-value:7.53E-7||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP1(YAR002C-A)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:ESL2(YKR096W)|FD-Score:4.41|P-value:5.18E-6||SGD DESC:Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain Gene:GLN3(YER040W)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GOT1(YMR292W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:HRD1(YOL013C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:HUA1(YGR268C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IES3(YLR052W)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IMP2'(YIL154C)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LAP3(YNL239W)|FD-Score:-3.87|P-value:5.44E-5||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LOT5(YKL183W)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:LRS4(YDR439W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MAC1(YMR021C)|FD-Score:3.75|P-value:8.69E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET1(YKR069W)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MFT1(YML062C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MNL2(YLR057W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:NAS2(YIL007C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:OAR1(YKL055C)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OCA1(YNL099C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OPI1(YHL020C)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PAU17(YLL025W)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PCL8(YPL219W)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PIL1(YGR086C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PKR1(YMR123W)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPH3(YDR075W)|FD-Score:4.85|P-value:6.07E-7||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:RAD1(YPL022W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RPL16A(YIL133C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS21B(YJL136C)|FD-Score:4.86|P-value:5.92E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RSC1(YGR056W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT109(YLL002W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SAS3(YBL052C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SCP160(YJL080C)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCT1(YBL011W)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SGE1(YPR198W)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SGN1(YIR001C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SLO1(YER180C-A)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SNF3(YDL194W)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:5.7|P-value:6.07E-9||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TMA23(YMR269W)|FD-Score:4.34|P-value:7.11E-6||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TPS2(YDR074W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRM2(YKR056W)|FD-Score:3.76|P-value:8.49E-5||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:UBI4(YLL039C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBX7(YBR273C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:VBA3(YCL069W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VFA1(YER128W)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VID28(YIL017C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPS51(YKR020W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:WHI3(YNL197C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBL095W(YBL095W_p)|FD-Score:4.41|P-value:5.06E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR206W(YBR206W_d)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YDR132C(YDR132C_p)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Protein of unknown function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication Gene:YDR271C(YDR271C_d)|FD-Score:4.03|P-value:2.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YGR130C(YGR130C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7) Gene:YIL163C(YIL163C_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL097C(YKL097C_d)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKR005C(YKR005C_p)|FD-Score:5.01|P-value:2.75E-7||SGD DESC:Putative protein of unknown function Gene:YKR104W(YKR104W)|FD-Score:5.17|P-value:1.17E-7||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YPR014C(YPR014C_d)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR150W(YPR150W_d)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C Gene:YPS3(YLR121C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPS6(YIR039C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT53(YNL093W)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

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Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL167W4.327.67E-60.25ERG20Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
YFR028C4.327.69E-60.25CDC14Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis
YAL041W4.171.49E-50.25CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YNR046W3.973.59E-50.25TRM112Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress
YJR064W3.729.91E-50.02CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR172W3.701.06E-40.01SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YOR119C3.691.11E-40.09RIO1Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA
YHR107C3.601.59E-40.14CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YDL150W3.462.71E-40.17RPC53RNA polymerase III subunit C53
YJR093C3.294.95E-40.08FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YKL186C3.216.66E-40.02MTR2mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
YDR201W3.197.19E-40.00SPC19Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
YLR100W3.197.23E-40.06ERG273-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
YDL166C3.138.80E-40.17FAP7Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D
YGR095C2.960.001550.01RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER167W7.232.43E-13BCK2Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations
YNL081C5.706.07E-9SWS2Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency
YKR104W5.171.17E-7YKR104WPutative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds
YMR299C5.141.34E-7DYN3Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
YHL020C5.121.52E-7OPI1Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
YAR002C-A5.062.11E-7ERP1Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
YKR005C_p5.012.75E-7YKR005C_pPutative protein of unknown function
YGR204W4.983.11E-7ADE3Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
YJL136C4.865.92E-7RPS21BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
YDR075W4.856.07E-7PPH3Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
YER128W4.836.77E-7VFA1Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology
YGL012W4.817.53E-7ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YKL183W4.741.08E-6LOT5Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress
YIR039C4.691.35E-6YPS6Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance
YIL133C4.641.71E-6RPL16ARibosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1155
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0823.50E-10SGTC_1812st052057 4.3 μMTimTec (Natural product derivative library)36454180.0481928calcium & mitochondrial duress
0.0765.77E-9SGTC_1230083-0120 66.7 μMChemDiv (Drug-like library)160017010.0769231
0.0757.62E-9SGTC_330986-0033 35.8 μMChemDiv (Drug-like library)28480820.104478calcium & mitochondrial duress
0.0741.21E-8SGTC_13772944-0135 198.0 μMChemDiv (Drug-like library)18941370.08calcium & mitochondrial duress
0.0732.34E-8SGTC_30889117578 49.5 μMChembridge (Drug-like library)6783410.0491803amide catabolism
0.0723.54E-8SGTC_32449134489 49.5 μMChembridge (Drug-like library)165842710.0657895
0.0707.04E-8SGTC_1463448-8650 53.1 μMChemDiv (Drug-like library)28914880.0493827NEO1-PIK1
0.0691.04E-7SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.0882353ERG2
0.0691.13E-7SGTC_1666st014829 29.0 μMTimTec (Natural product derivative library)38582300.0625calcium & mitochondrial duress
0.0691.29E-7SGTC_550767-0285 6.6 μMChemDiv (Drug-like library)31161660.133333
0.0672.86E-7SGTC_400oxethazaine 37.5 μMMiscellaneous46210.112903calcium & mitochondrial duress
0.0596.42E-6SGTC_22677931562 200.0 μMChembridge (Fragment library)9348800.0952381
0.0596.41E-6SGTC_1163k060-0048 49.9 μMChemDiv (Drug-like library)68099820.078125
0.0596.83E-6SGTC_31989112019 49.5 μMChembridge (Drug-like library)455955550.0714286mitochondrial processes
0.0587.07E-6SGTC_1621st003705 32.4 μMTimTec (Natural product derivative library)242070320.111111calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20865274770176.62 μM0.282609689278Chembridge (Fragment library)254.31031.46325
SGTC_2350902389538.85 μM0.2692312997209Chembridge (Fragment library)266.337622.29402ergosterol biosynthesis
SGTC_3124092-06536.84 μM0.2631581148278ChemDiv (Drug-like library)366.4716834.54903ergosterol depletion effects on membrane
SGTC_14754092-104811.7 μM0.2592592881759ChemDiv (Drug-like library)338.529465.02102plasma membrane duress
SGTC_1651st01192886.1 μM0.259259720198TimTec (Natural product derivative library)232.258421.77414RSC complex & mRNA processing
SGTC_10194262-02123.4 μM0.254677213ChemDiv (Drug-like library)352.4881635.20803ergosterol depletion effects on membrane
SGTC_11564092-03674.88 μM0.251357585ChemDiv (Drug-like library)382.926285.00702
SGTC_14214029-024971.4 μM0.252894095ChemDiv (Drug-like library)283.3400233.71802
SGTC_2035515446394.6 μM0.2456142769623Chembridge (Fragment library)254.283861.48914
SGTC_12780874-0585172 μM0.244898766306ChemDiv (Drug-like library)234.360424.3721