4092-0394

2-(4-chlorophenoxy)-1-[4-[(3-methylphenyl)methyl]piperazin-1-yl]ethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1157
Screen concentration 27.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 3659885
SMILES CC1=CC(=CC=C1)CN2CCN(CC2)C(=O)COC3=CC=C(C=C3)Cl
Standardized SMILES Cc1cccc(CN2CCN(CC2)C(=O)COc3ccc(Cl)cc3)c1
Molecular weight 358.8618
ALogP 3.77
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.93
% growth inhibition (Hom. pool) 3.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3659885
Download HIP data (tab-delimited text)  (excel)
Gene:ALA1(YOR335C)|FD-Score:3.14|P-value:8.59E-4|Clearance:0.02||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:CDC11(YJR076C)|FD-Score:-4.87|P-value:5.48E-7|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:NOP15(YNL110C)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.14||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PAM16(YJL104W)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.07||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PGI1(YBR196C)|FD-Score:3.12|P-value:9.05E-4|Clearance:0.01||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PRP45(YAL032C)|FD-Score:4.85|P-value:6.13E-7|Clearance:0.68||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RPO21(YDL140C)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.16||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:RPS20(YHL015W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.09||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SMP3(YOR149C)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.19||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SRP68(YPL243W)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.01||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:TAF5(YBR198C)|FD-Score:6.04|P-value:7.58E-10|Clearance:1.19||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:UTP20(YBL004W)|FD-Score:4.17|P-value:1.53E-5|Clearance:0.45||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:ALA1(YOR335C)|FD-Score:3.14|P-value:8.59E-4|Clearance:0.02||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:CDC11(YJR076C)|FD-Score:-4.87|P-value:5.48E-7|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:NOP15(YNL110C)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.14||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PAM16(YJL104W)|FD-Score:3.1|P-value:9.73E-4|Clearance:0.07||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PGI1(YBR196C)|FD-Score:3.12|P-value:9.05E-4|Clearance:0.01||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PRP45(YAL032C)|FD-Score:4.85|P-value:6.13E-7|Clearance:0.68||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:RPO21(YDL140C)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.16||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:RPS20(YHL015W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.09||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SMP3(YOR149C)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.19||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SRP68(YPL243W)|FD-Score:3.11|P-value:9.26E-4|Clearance:0.01||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:TAF5(YBR198C)|FD-Score:6.04|P-value:7.58E-10|Clearance:1.19||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:UTP20(YBL004W)|FD-Score:4.17|P-value:1.53E-5|Clearance:0.45||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3659885
Download HOP data (tab-delimited text)  (excel)
Gene:AHC2(YCR082W)|FD-Score:6.95|P-value:1.84E-12||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:APM1(YPL259C)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:APN1(YKL114C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ATG1(YGL180W)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATP4(YPL078C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BCS1(YDR375C)|FD-Score:4.76|P-value:9.70E-7||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BIO5(YNR056C)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BTS1(YPL069C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAN1(YEL063C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CIN2(YPL241C)|FD-Score:5.11|P-value:1.57E-7||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLN3(YAL040C)|FD-Score:5.3|P-value:5.79E-8||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COA3(YJL062W-A)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:HIR1(YBL008W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HMS2(YJR147W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HOF1(YMR032W)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HPR1(YDR138W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IFM1(YOL023W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRC13(YOR235W_d)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:KAR9(YPL269W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LHS1(YKL073W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MDJ2(YNL328C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MEI4(YER044C-A)|FD-Score:5.59|P-value:1.12E-8||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MRI1(YPR118W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRPS8(YMR158W)|FD-Score:6.14|P-value:4.16E-10||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSF1(YPR047W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSH3(YCR092C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NDI1(YML120C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:PDR12(YPL058C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PNG1(YPL096W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PRY3(YJL078C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:RGL1(YPL066W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL7A(YGL076C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:SAC1(YKL212W)|FD-Score:4.3|P-value:8.50E-6||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SET5(YHR207C)|FD-Score:4.33|P-value:7.44E-6||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SOL2(YCR073W-A)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SRB2(YHR041C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SSD1(YDR293C)|FD-Score:4.83|P-value:6.67E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STB2(YMR053C)|FD-Score:6|P-value:1.01E-9||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SUR4(YLR372W)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:THI7(YLR237W)|FD-Score:4.45|P-value:4.20E-6||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:THO2(YNL139C)|FD-Score:5.61|P-value:1.01E-8||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TPS2(YDR074W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.01|P-value:9.07E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUS1(YLR425W)|FD-Score:5.32|P-value:5.25E-8||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBA4(YHR111W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VMA1(YDL185W)|FD-Score:5.68|P-value:6.78E-9||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS21(YOR089C)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS9(YML097C)|FD-Score:6.73|P-value:8.38E-12||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YDR239C(YDR239C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YFR035C(YFR035C_p)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YKL118W(YKL118W_d)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR104W(YKR104W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR419W(YLR419W_p)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YML009W-B(YML009W-B_d)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.49|P-value:3.61E-6||SGD DESC:Putative protein of unknown function Gene:YNL203C(YNL203C_d)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR048W(YNR048W)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOR343C(YOR343C_d)|FD-Score:3.96|P-value:3.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:AHC2(YCR082W)|FD-Score:6.95|P-value:1.84E-12||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:APM1(YPL259C)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:APN1(YKL114C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ATG1(YGL180W)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATP4(YPL078C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BCS1(YDR375C)|FD-Score:4.76|P-value:9.70E-7||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:BIO5(YNR056C)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BTS1(YPL069C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAN1(YEL063C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CIN2(YPL241C)|FD-Score:5.11|P-value:1.57E-7||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLN3(YAL040C)|FD-Score:5.3|P-value:5.79E-8||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COA3(YJL062W-A)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:HIR1(YBL008W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HMS2(YJR147W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication Gene:HOF1(YMR032W)|FD-Score:4.72|P-value:1.18E-6||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HPR1(YDR138W)|FD-Score:4|P-value:3.18E-5||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IFM1(YOL023W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRC13(YOR235W_d)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:KAR9(YPL269W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LHS1(YKL073W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MDJ2(YNL328C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MEI4(YER044C-A)|FD-Score:5.59|P-value:1.12E-8||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MRI1(YPR118W)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRPS8(YMR158W)|FD-Score:6.14|P-value:4.16E-10||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSF1(YPR047W)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSH3(YCR092C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NDI1(YML120C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:PDR12(YPL058C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PNG1(YPL096W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PRY3(YJL078C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:RGL1(YPL066W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL7A(YGL076C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPS14A(YCR031C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:SAC1(YKL212W)|FD-Score:4.3|P-value:8.50E-6||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SET5(YHR207C)|FD-Score:4.33|P-value:7.44E-6||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SOL2(YCR073W-A)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SRB2(YHR041C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SSD1(YDR293C)|FD-Score:4.83|P-value:6.67E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STB2(YMR053C)|FD-Score:6|P-value:1.01E-9||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SUR4(YLR372W)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:THI7(YLR237W)|FD-Score:4.45|P-value:4.20E-6||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:THO2(YNL139C)|FD-Score:5.61|P-value:1.01E-8||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TPS2(YDR074W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.01|P-value:9.07E-10||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUS1(YLR425W)|FD-Score:5.32|P-value:5.25E-8||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBA4(YHR111W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VMA1(YDL185W)|FD-Score:5.68|P-value:6.78E-9||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS21(YOR089C)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS9(YML097C)|FD-Score:6.73|P-value:8.38E-12||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YDR239C(YDR239C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YFR035C(YFR035C_p)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YKL118W(YKL118W_d)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR104W(YKR104W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR419W(YLR419W_p)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YML009W-B(YML009W-B_d)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.49|P-value:3.61E-6||SGD DESC:Putative protein of unknown function Gene:YNL203C(YNL203C_d)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR048W(YNR048W)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOR343C(YOR343C_d)|FD-Score:3.96|P-value:3.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR198C6.047.58E-101.19TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YAL032C4.856.13E-70.68PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YBL004W4.171.53E-50.45UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YOR149C3.711.02E-40.19SMP3Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
YHL015W3.532.09E-40.09RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YDL140C3.442.88E-40.16RPO21RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
YNL110C3.285.19E-40.14NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YOR335C3.148.59E-40.02ALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
YBR196C3.129.05E-40.01PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YPL243W3.119.26E-40.01SRP68Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YJL104W3.109.73E-40.07PAM16Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
YJL041W3.030.001240.02NSP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress
YNL006W3.010.001310.05LST8Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
YJL011C2.960.001550.09RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YDL017W2.870.002030.02CDC7DDK (Dbf4-dependent kinase) catalytic subunit required for origin firing and replication fork progression in mitotic S phase through phosphorylation of Mcm2-7p complexes and Cdc45p; kinase activity correlates with cyclical DBF4 expression; required for pre-meiotic DNA replication, meiotic DSB formation, recruitment of the monopolin complex to kinetochores during meiosis I and as a gene-specific regulator of the meiosis-specific transcription factor Ndt80p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR082W6.951.84E-12AHC2Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex
YML097C6.738.38E-12VPS9A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
YMR158W6.144.16E-10MRPS8Mitochondrial ribosomal protein of the small subunit
YER090W6.019.07E-10TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YMR053C6.001.01E-9STB2Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p
YDL185W5.686.78E-9VMA1Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
YNL139C5.611.01E-8THO2Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
YER044C-A5.591.12E-8MEI4Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores
YLR425W5.325.25E-8TUS1Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
YAL040C5.305.79E-8CLN3G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis
YPL241C5.111.57E-7CIN2GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
YNL203C_d4.894.93E-7YNL203C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR293C4.836.67E-7SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YDR375C4.769.70E-7BCS1Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
YMR032W4.721.18E-6HOF1Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p

GO enrichment analysis for SGTC_1157
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1425.17E-28SGTC_21635671233 125.0 μMChembridge (Fragment library)7010230.0921053
0.1402.46E-27SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.162162
0.1402.82E-27SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0806452
0.1344.77E-25SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.109589
0.1316.66E-24SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.142857
0.1317.50E-24SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.169014
0.1293.00E-23SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.233766
0.1253.94E-22SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.140845
0.1226.06E-21SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.266667
0.1217.79E-21SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.121212
0.1219.23E-21SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0506329
0.1202.18E-20SGTC_11594092-0538 61.2 μMChemDiv (Drug-like library)7173010.396552
0.1202.63E-20SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.164179
0.1196.25E-20SGTC_24545-benzylidenerhodanine 10.4 μMMiscellaneous12732100.0735294TRP & mitochondrial translation
0.1181.19E-19SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.148649

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_11604092-057010.5 μM0.6481484994975ChemDiv (Drug-like library)390.926823.82604plasma membrane duress
SGTC_3049909222349.47 μM0.5636368623917Chembridge (Drug-like library)328.835843.93502
SGTC_3033909088749.47 μM0.49090919325384Chembridge (Drug-like library)342.862423.9402fatty acid desaturase (OLE1)
SGTC_3051909377849.47 μM0.48333317217378Chembridge (Drug-like library)338.443343.5903
SGTC_3045909295049.47 μM0.48275944120432Chembridge (Drug-like library)365.296784.31712
SGTC_3140909424349.47 μM0.4754119291112Chembridge (Drug-like library)342.4072233.32204
SGTC_3137909735249.47 μM0.46666744120435Chembridge (Drug-like library)371.32453.9313
SGTC_13752910-0797186 μM0.465517742508ChemDiv (Drug-like library)340.416162.75204
SGTC_5154092-1064102 μM0.46717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress
SGTC_14394092-116846 μM0.4098362886240ChemDiv (Drug-like library)346.851123.8514