k007-0610

2,4-difluoro-N-[(Z)-1-naphthalen-2-ylethylideneamino]aniline

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1161
Screen concentration 110.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5843850
SMILES CC(=NNC1=C(C=C(C=C1)F)F)C2=CC3=CC=CC=C3C=C2
Standardized SMILES CC(=NNc1ccc(F)cc1F)c2ccc3ccccc3c2
Molecular weight 296.314
ALogP 4.75
H-bond donor count 1
H-bond acceptor count 4
Response signature ERAD & cell cycle

Pool Growth Kinetics
% growth inhibition (Het. pool) 36.96
% growth inhibition (Hom. pool) 36.42


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5843850
Download HIP data (tab-delimited text)  (excel)
Gene:BRL1(YHR036W)|FD-Score:-7.04|P-value:9.47E-13|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC20(YGL116W)|FD-Score:16.8|P-value:1.42E-63|Clearance:2.34||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC36(YDL165W)|FD-Score:4.05|P-value:2.56E-5|Clearance:0.03||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC39(YCR093W)|FD-Score:6.31|P-value:1.43E-10|Clearance:0.15||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC48(YDL126C)|FD-Score:9.11|P-value:4.17E-20|Clearance:0.33||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CDC60(YPL160W)|FD-Score:4.34|P-value:7.06E-6|Clearance:0.09||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:COP1(YDL145C)|FD-Score:6.59|P-value:2.15E-11|Clearance:0.27||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DML1(YMR211W)|FD-Score:-4.75|P-value:1.01E-6|Clearance:0||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:8.21|P-value:1.08E-16|Clearance:0.05||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAS1(YKL182W)|FD-Score:5.53|P-value:1.57E-8|Clearance:0.11||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GPI14(YJR013W)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.07||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HSP10(YOR020C)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.02||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:HYP2(YEL034W)|FD-Score:-3.25|P-value:5.68E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IDI1(YPL117C)|FD-Score:4.48|P-value:3.80E-6|Clearance:0||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:IRA1(YBR140C)|FD-Score:5.19|P-value:1.03E-7|Clearance:0.01||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:3.87|P-value:5.49E-5|Clearance:0.05||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:-3.3|P-value:4.77E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MED8(YBR193C)|FD-Score:4.67|P-value:1.50E-6|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MRS6(YOR370C)|FD-Score:6.16|P-value:3.67E-10|Clearance:0.34||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:NAN1(YPL126W)|FD-Score:3.82|P-value:6.68E-5|Clearance:0.28||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NEO1(YIL048W)|FD-Score:7.04|P-value:9.49E-13|Clearance:0.45||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP14(YDL148C)|FD-Score:22|P-value:6.80E-108|Clearance:5.24||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP15(YNL110C)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP56(YLR197W)|FD-Score:-4.4|P-value:5.50E-6|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:8.78|P-value:8.16E-19|Clearance:0.09||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:OLE1(YGL055W)|FD-Score:4.02|P-value:2.89E-5|Clearance:0.15||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PAM16(YJL104W)|FD-Score:3.22|P-value:6.33E-4|Clearance:0.08||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PGA2(YNL149C)|FD-Score:7.82|P-value:2.71E-15|Clearance:0.6||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:PRP24(YMR268C)|FD-Score:5.24|P-value:8.12E-8|Clearance:0.04||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RAD53(YPL153C)|FD-Score:4.58|P-value:2.28E-6|Clearance:0.11||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RET2(YFR051C)|FD-Score:4.8|P-value:7.83E-7|Clearance:0.02||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RET3(YPL010W)|FD-Score:5.18|P-value:1.09E-7|Clearance:0.06||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPL25(YOL127W)|FD-Score:7.22|P-value:2.57E-13|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPN5(YDL147W)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN8(YOR261C)|FD-Score:-4.13|P-value:1.85E-5|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPS20(YHL015W)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RSC6(YCR052W)|FD-Score:4.36|P-value:6.51E-6|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SAM35(YHR083W)|FD-Score:4.16|P-value:1.56E-5|Clearance:0.11||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SAM50(YNL026W)|FD-Score:3.14|P-value:8.42E-4|Clearance:0.07||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SEC21(YNL287W)|FD-Score:14.3|P-value:1.25E-46|Clearance:5.18||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC24(YIL109C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SEC26(YDR238C)|FD-Score:4.79|P-value:8.48E-7|Clearance:0.12||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC39(YLR440C)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.06||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SEC59(YMR013C)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.04||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:SEC65(YML105C)|FD-Score:8.17|P-value:1.60E-16|Clearance:0.07||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SPC105(YGL093W)|FD-Score:6.32|P-value:1.28E-10|Clearance:0.02||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPC29(YPL124W)|FD-Score:4.47|P-value:3.88E-6|Clearance:0.11||SGD DESC:Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication Gene:SPT14(YPL175W)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.12||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SPT6(YGR116W)|FD-Score:-3.11|P-value:9.35E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SRP68(YPL243W)|FD-Score:5.42|P-value:2.92E-8|Clearance:0.19||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:SRP72(YPL210C)|FD-Score:5.12|P-value:1.49E-7|Clearance:0.32||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TFA1(YKL028W)|FD-Score:-5.19|P-value:1.04E-7|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFC3(YAL001C)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.05||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TFC6(YDR362C)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.1||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIM17(YJL143W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.01||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM44(YIL022W)|FD-Score:5.82|P-value:2.92E-9|Clearance:0.29||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:TIM54(YJL054W)|FD-Score:-3.79|P-value:7.50E-5|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOA2(YKL058W)|FD-Score:8.69|P-value:1.80E-18|Clearance:0.48||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TOM40(YMR203W)|FD-Score:4.2|P-value:1.32E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YBR089W(YBR089W_d)|FD-Score:3.34|P-value:4.12E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Gene:YGL074C(YGL074C_d)|FD-Score:4.36|P-value:6.48E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YGR114C(YGR114C_d)|FD-Score:-4.88|P-value:5.28E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:-3.88|P-value:5.21E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YGR265W(YGR265W_d)|FD-Score:-4.58|P-value:2.28E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJL086C(YJL086C_d)|FD-Score:8.09|P-value:2.91E-16|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YTH1(YPR107C)|FD-Score:14.4|P-value:1.32E-47|Clearance:0.16||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:BRL1(YHR036W)|FD-Score:-7.04|P-value:9.47E-13|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC20(YGL116W)|FD-Score:16.8|P-value:1.42E-63|Clearance:2.34||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC36(YDL165W)|FD-Score:4.05|P-value:2.56E-5|Clearance:0.03||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC39(YCR093W)|FD-Score:6.31|P-value:1.43E-10|Clearance:0.15||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC48(YDL126C)|FD-Score:9.11|P-value:4.17E-20|Clearance:0.33||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CDC60(YPL160W)|FD-Score:4.34|P-value:7.06E-6|Clearance:0.09||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:COP1(YDL145C)|FD-Score:6.59|P-value:2.15E-11|Clearance:0.27||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DML1(YMR211W)|FD-Score:-4.75|P-value:1.01E-6|Clearance:0||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:8.21|P-value:1.08E-16|Clearance:0.05||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAS1(YKL182W)|FD-Score:5.53|P-value:1.57E-8|Clearance:0.11||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GPI14(YJR013W)|FD-Score:4.65|P-value:1.62E-6|Clearance:0.07||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:HSP10(YOR020C)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.02||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:HYP2(YEL034W)|FD-Score:-3.25|P-value:5.68E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IDI1(YPL117C)|FD-Score:4.48|P-value:3.80E-6|Clearance:0||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:IRA1(YBR140C)|FD-Score:5.19|P-value:1.03E-7|Clearance:0.01||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:3.87|P-value:5.49E-5|Clearance:0.05||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:-3.3|P-value:4.77E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MED8(YBR193C)|FD-Score:4.67|P-value:1.50E-6|Clearance:0.01||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MRS6(YOR370C)|FD-Score:6.16|P-value:3.67E-10|Clearance:0.34||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:NAN1(YPL126W)|FD-Score:3.82|P-value:6.68E-5|Clearance:0.28||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NEO1(YIL048W)|FD-Score:7.04|P-value:9.49E-13|Clearance:0.45||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP14(YDL148C)|FD-Score:22|P-value:6.80E-108|Clearance:5.24||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP15(YNL110C)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP56(YLR197W)|FD-Score:-4.4|P-value:5.50E-6|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:8.78|P-value:8.16E-19|Clearance:0.09||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:OLE1(YGL055W)|FD-Score:4.02|P-value:2.89E-5|Clearance:0.15||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PAM16(YJL104W)|FD-Score:3.22|P-value:6.33E-4|Clearance:0.08||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PGA2(YNL149C)|FD-Score:7.82|P-value:2.71E-15|Clearance:0.6||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:PRP24(YMR268C)|FD-Score:5.24|P-value:8.12E-8|Clearance:0.04||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RAD53(YPL153C)|FD-Score:4.58|P-value:2.28E-6|Clearance:0.11||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RET2(YFR051C)|FD-Score:4.8|P-value:7.83E-7|Clearance:0.02||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RET3(YPL010W)|FD-Score:5.18|P-value:1.09E-7|Clearance:0.06||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPL25(YOL127W)|FD-Score:7.22|P-value:2.57E-13|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPN5(YDL147W)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN8(YOR261C)|FD-Score:-4.13|P-value:1.85E-5|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPS20(YHL015W)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RSC6(YCR052W)|FD-Score:4.36|P-value:6.51E-6|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SAM35(YHR083W)|FD-Score:4.16|P-value:1.56E-5|Clearance:0.11||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SAM50(YNL026W)|FD-Score:3.14|P-value:8.42E-4|Clearance:0.07||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SEC21(YNL287W)|FD-Score:14.3|P-value:1.25E-46|Clearance:5.18||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC24(YIL109C)|FD-Score:4.66|P-value:1.58E-6|Clearance:0||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SEC26(YDR238C)|FD-Score:4.79|P-value:8.48E-7|Clearance:0.12||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC39(YLR440C)|FD-Score:3.4|P-value:3.32E-4|Clearance:0.06||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SEC59(YMR013C)|FD-Score:3.44|P-value:2.87E-4|Clearance:0.04||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:SEC65(YML105C)|FD-Score:8.17|P-value:1.60E-16|Clearance:0.07||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SPC105(YGL093W)|FD-Score:6.32|P-value:1.28E-10|Clearance:0.02||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPC29(YPL124W)|FD-Score:4.47|P-value:3.88E-6|Clearance:0.11||SGD DESC:Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication Gene:SPT14(YPL175W)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.12||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SPT6(YGR116W)|FD-Score:-3.11|P-value:9.35E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SRP68(YPL243W)|FD-Score:5.42|P-value:2.92E-8|Clearance:0.19||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:SRP72(YPL210C)|FD-Score:5.12|P-value:1.49E-7|Clearance:0.32||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TFA1(YKL028W)|FD-Score:-5.19|P-value:1.04E-7|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFC3(YAL001C)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.05||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:TFC6(YDR362C)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.1||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:TIM17(YJL143W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.01||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM44(YIL022W)|FD-Score:5.82|P-value:2.92E-9|Clearance:0.29||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:TIM54(YJL054W)|FD-Score:-3.79|P-value:7.50E-5|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOA2(YKL058W)|FD-Score:8.69|P-value:1.80E-18|Clearance:0.48||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TOM40(YMR203W)|FD-Score:4.2|P-value:1.32E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YBR089W(YBR089W_d)|FD-Score:3.34|P-value:4.12E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Gene:YGL074C(YGL074C_d)|FD-Score:4.36|P-value:6.48E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YGR114C(YGR114C_d)|FD-Score:-4.88|P-value:5.28E-7|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:-3.88|P-value:5.21E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YGR265W(YGR265W_d)|FD-Score:-4.58|P-value:2.28E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YJL086C(YJL086C_d)|FD-Score:8.09|P-value:2.91E-16|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YTH1(YPR107C)|FD-Score:14.4|P-value:1.32E-47|Clearance:0.16||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5843850
Download HOP data (tab-delimited text)  (excel)
Gene:ACF4(YJR083C)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:AEP3(YPL005W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AHC1(YOR023C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ALD2(YMR170C)|FD-Score:5.56|P-value:1.31E-8||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ALF1(YNL148C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ALG6(YOR002W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ALG8(YOR067C)|FD-Score:-5.58|P-value:1.22E-8||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APA2(YDR530C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication Gene:APM1(YPL259C)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARF1(YDL192W)|FD-Score:6.37|P-value:9.59E-11||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASC1(YMR116C)|FD-Score:6.5|P-value:3.91E-11||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ATG13(YPR185W)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATG15(YCR068W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BCK1(YJL095W)|FD-Score:6.53|P-value:3.33E-11||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BCK2(YER167W)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BTS1(YPL069C)|FD-Score:5.25|P-value:7.52E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD3(YCL014W)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CCW12(YLR110C)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CKA2(YOR061W)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CKB1(YGL019W)|FD-Score:11.9|P-value:3.73E-33||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:7.48|P-value:3.70E-14||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNB1(YKL190W)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COX15(YER141W)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:CSF1(YLR087C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSG2(YBR036C)|FD-Score:7.51|P-value:2.90E-14||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSR1(YLR380W)|FD-Score:13.8|P-value:8.22E-44||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:5.26|P-value:7.27E-8||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:5.56|P-value:1.37E-8||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DCS1(YLR270W)|FD-Score:-6.75|P-value:7.50E-12||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DGR2(YKL121W_p)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DLT1(YMR126C_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:DOA1(YKL213C)|FD-Score:4|P-value:3.23E-5||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DSK2(YMR276W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:EAF7(YNL136W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ENV10(YLR065C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ENV9(YOR246C)|FD-Score:15.2|P-value:2.49E-52||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERG2(YMR202W)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FAP1(YNL023C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FAR10(YLR238W)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FAR11(YNL127W)|FD-Score:4.62|P-value:1.89E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FAR3(YMR052W)|FD-Score:7.35|P-value:1.01E-13||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FAR7(YFR008W)|FD-Score:10.2|P-value:1.35E-24||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FAR8(YMR029C)|FD-Score:8.87|P-value:3.65E-19||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FBP26(YJL155C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:5.55|P-value:1.40E-8||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRA1(YLL029W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GAL83(YER027C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GCN3(YKR026C)|FD-Score:6.83|P-value:4.34E-12||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCS1(YDL226C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEF1(YJR040W)|FD-Score:6.67|P-value:1.31E-11||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GET2(YER083C)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:13.1|P-value:1.71E-39||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GGC1(YDL198C)|FD-Score:-3.79|P-value:7.61E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GPA2(YER020W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPR1(YDL035C)|FD-Score:6.5|P-value:3.96E-11||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GRE3(YHR104W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GSH1(YJL101C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:9.28|P-value:8.11E-21||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOG1(YLR113W)|FD-Score:5.44|P-value:2.70E-8||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HOM6(YJR139C)|FD-Score:-4.4|P-value:5.40E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HPM1(YIL110W)|FD-Score:-4.81|P-value:7.43E-7||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HRD1(YOL013C)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:ICE2(YIL090W)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IES1(YFL013C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IME4(YGL192W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:INP54(YOL065C)|FD-Score:10.1|P-value:3.97E-24||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:IPT1(YDR072C)|FD-Score:5.62|P-value:9.46E-9||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRS4(YKR019C)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:IZH2(YOL002C)|FD-Score:10.4|P-value:1.17E-25||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KGD1(YIL125W)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:KHA1(YJL094C)|FD-Score:6.03|P-value:8.05E-10||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:LAP3(YNL239W)|FD-Score:7.55|P-value:2.15E-14||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LHS1(YKL073W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LPD1(YFL018C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MAL33(YBR297W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MCA1(YOR197W)|FD-Score:4.29|P-value:8.77E-6||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MIG3(YER028C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MMT1(YMR177W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MNL2(YLR057W)|FD-Score:6.54|P-value:3.07E-11||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MOT3(YMR070W)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRH4(YGL064C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP21(YBL090W)|FD-Score:-5.47|P-value:2.29E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL28(YDR462W)|FD-Score:-3.67|P-value:1.24E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL7(YDR237W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS5(YBR251W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRS3(YJL133W)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MRS4(YKR052C)|FD-Score:-5.65|P-value:7.94E-9||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MSM1(YGR171C)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MSN1(YOL116W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MSW1(YDR268W)|FD-Score:-4.65|P-value:1.65E-6||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTC2(YKL098W)|FD-Score:5.6|P-value:1.06E-8||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MTC4(YBR255W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MTF1(YMR228W)|FD-Score:-3.89|P-value:5.06E-5||SGD DESC:Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:MTM1(YGR257C)|FD-Score:-4.85|P-value:6.18E-7||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NEM1(YHR004C)|FD-Score:8.18|P-value:1.41E-16||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NUP170(YBL079W)|FD-Score:5.39|P-value:3.58E-8||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OAF1(YAL051W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OSH3(YHR073W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OYE2(YHR179W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PCL6(YER059W)|FD-Score:5.3|P-value:5.64E-8||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PEP8(YJL053W)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX29(YDR479C)|FD-Score:4.86|P-value:5.74E-7||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PGD1(YGL025C)|FD-Score:-3.85|P-value:5.82E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO88(YBR106W)|FD-Score:6.07|P-value:6.27E-10||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PHO89(YBR296C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIM1(YBL022C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PIN2(YOR104W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PIP2(YOR363C)|FD-Score:6.94|P-value:1.92E-12||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PPM1(YDR435C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRE9(YGR135W)|FD-Score:9.89|P-value:2.26E-23||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PSP2(YML017W)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTC1(YDL006W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTC6(YCR079W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:PUB1(YNL016W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:QRI5(YLR204W)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RBG1(YAL036C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RCY1(YJL204C)|FD-Score:7.47|P-value:3.94E-14||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHO5(YNL180C)|FD-Score:5.49|P-value:2.02E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RHR2(YIL053W)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RML2(YEL050C)|FD-Score:-4.91|P-value:4.50E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROY1(YMR258C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL12B(YDR418W)|FD-Score:4.28|P-value:9.37E-6||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:6.89|P-value:2.79E-12||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:18.2|P-value:1.59E-74||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTF1(YGL244W)|FD-Score:-6.43|P-value:6.29E-11||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTK1(YDL025C)|FD-Score:8.58|P-value:4.82E-18||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:RTT10(YPL183C)|FD-Score:-5.44|P-value:2.71E-8||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:RTT106(YNL206C)|FD-Score:4.39|P-value:5.60E-6||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAC6(YDR129C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAP190(YKR028W)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SBH2(YER019C-A)|FD-Score:6.14|P-value:4.05E-10||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SCP160(YJL080C)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCS3(YGL126W)|FD-Score:8.15|P-value:1.78E-16||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SEC22(YLR268W)|FD-Score:8.73|P-value:1.22E-18||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEY1(YOR165W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SGF73(YGL066W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIF2(YBR103W)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SIN3(YOL004W)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SNF3(YDL194W)|FD-Score:6.49|P-value:4.33E-11||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SOD2(YHR008C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPO7(YAL009W)|FD-Score:7.05|P-value:9.14E-13||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPT23(YKL020C)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SPT3(YDR392W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRB2(YHR041C)|FD-Score:-4.64|P-value:1.76E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRC1(YML034W)|FD-Score:7.46|P-value:4.27E-14||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SRF1(YDL133W)|FD-Score:6.33|P-value:1.26E-10||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSD1(YDR293C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSM4(YIL030C)|FD-Score:27.5|P-value:6.58E-167||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SSN2(YDR443C)|FD-Score:-5.25|P-value:7.59E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSP120(YLR250W)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SSQ1(YLR369W)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:STE24(YJR117W)|FD-Score:7.05|P-value:9.18E-13||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SVF1(YDR346C)|FD-Score:10.3|P-value:2.54E-25||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SWS2(YNL081C)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TGL3(YMR313C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TLG2(YOL018C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM7(YNL070W)|FD-Score:4.32|P-value:7.92E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRM9(YML014W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP1(YDR007W)|FD-Score:6.56|P-value:2.68E-11||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.5|P-value:4.11E-11||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:12.4|P-value:8.40E-36||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.22|P-value:2.55E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS65(YGR166W)|FD-Score:6.59|P-value:2.24E-11||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TVP18(YMR071C)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBC7(YMR022W)|FD-Score:19.6|P-value:1.68E-85||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UBP1(YDL122W)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBP14(YBR058C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UBP16(YPL072W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UBX4(YMR067C)|FD-Score:7.33|P-value:1.17E-13||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:UGA2(YBR006W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VHR1(YIL056W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication Gene:VPS51(YKR020W)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:4.37|P-value:6.27E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS64(YDR200C)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS74(YDR372C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBR226C(YBR226C_d)|FD-Score:6.51|P-value:3.74E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL001W-A(YCL001W-A_p)|FD-Score:9.07|P-value:5.90E-20||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR049C(YCR049C_d)|FD-Score:7.12|P-value:5.24E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR087C-A(YCR087C-A_p)|FD-Score:7.71|P-value:6.50E-15||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL183C(YDL183C)|FD-Score:4.47|P-value:3.95E-6||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR186C(YDR186C)|FD-Score:15.9|P-value:3.26E-57||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR199W(YDR199W_d)|FD-Score:7.58|P-value:1.69E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR442W(YDR442W_d)|FD-Score:-4.04|P-value:2.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR537C(YDR537C_d)|FD-Score:7.8|P-value:3.04E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEN1(YER041W)|FD-Score:12.8|P-value:1.36E-37||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER084W(YER084W_p)|FD-Score:15.3|P-value:3.18E-53||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFH7(YFR007W)|FD-Score:5.39|P-value:3.45E-8||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGL072C(YGL072C_d)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YJL193W(YJL193W_p)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YJR018W(YJR018W_d)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR129C(YJR129C_p)|FD-Score:7.62|P-value:1.26E-14||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YJU3(YKL094W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLL007C(YLL007C_p)|FD-Score:6.91|P-value:2.41E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR072W(YLR072W_p)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR111W(YLR111W_d)|FD-Score:5.3|P-value:5.92E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR407W(YLR407W_p)|FD-Score:8.12|P-value:2.32E-16||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YLR422W(YLR422W_p)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YML012C-A(YML012C-A_d)|FD-Score:4.47|P-value:3.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.82|P-value:7.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR057C(YMR057C_d)|FD-Score:4.39|P-value:5.77E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Putative protein of unknown function Gene:YNR005C(YNR005C_d)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOS9(YDR057W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPK1(YKL126W)|FD-Score:5.4|P-value:3.40E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR064W(YPR064W_d)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR153W(YPR153W)|FD-Score:4.76|P-value:9.54E-7||SGD DESC:Putative protein of unknown function Gene:YPT52(YKR014C)|FD-Score:6.84|P-value:3.94E-12||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:ACF4(YJR083C)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:AEP3(YPL005W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AHC1(YOR023C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ALD2(YMR170C)|FD-Score:5.56|P-value:1.31E-8||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ALF1(YNL148C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ALG6(YOR002W)|FD-Score:-4|P-value:3.12E-5||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ALG8(YOR067C)|FD-Score:-5.58|P-value:1.22E-8||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:APA2(YDR530C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication Gene:APM1(YPL259C)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ARF1(YDL192W)|FD-Score:6.37|P-value:9.59E-11||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASC1(YMR116C)|FD-Score:6.5|P-value:3.91E-11||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ATG13(YPR185W)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATG15(YCR068W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Lipase required for intravacuolar lysis of autophagic bodies and Cvt bodies; targeted to intravacuolar vesicles during autophagy via the multivesicular body (MVB) pathway Gene:BCK1(YJL095W)|FD-Score:6.53|P-value:3.33E-11||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BCK2(YER167W)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BTS1(YPL069C)|FD-Score:5.25|P-value:7.52E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD3(YCL014W)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CCW12(YLR110C)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CKA2(YOR061W)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CKB1(YGL019W)|FD-Score:11.9|P-value:3.73E-33||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:7.48|P-value:3.70E-14||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNB1(YKL190W)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:COX15(YER141W)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:CSF1(YLR087C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSG2(YBR036C)|FD-Score:7.51|P-value:2.90E-14||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSR1(YLR380W)|FD-Score:13.8|P-value:8.22E-44||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:5.26|P-value:7.27E-8||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:5.56|P-value:1.37E-8||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DCS1(YLR270W)|FD-Score:-6.75|P-value:7.50E-12||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DGR2(YKL121W_p)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DLT1(YMR126C_p)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:DOA1(YKL213C)|FD-Score:4|P-value:3.23E-5||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DSK2(YMR276W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:EAF7(YNL136W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ENV10(YLR065C)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ENV9(YOR246C)|FD-Score:15.2|P-value:2.49E-52||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERG2(YMR202W)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FAP1(YNL023C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FAR10(YLR238W)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FAR11(YNL127W)|FD-Score:4.62|P-value:1.89E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FAR3(YMR052W)|FD-Score:7.35|P-value:1.01E-13||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FAR7(YFR008W)|FD-Score:10.2|P-value:1.35E-24||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FAR8(YMR029C)|FD-Score:8.87|P-value:3.65E-19||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FBP26(YJL155C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:5.55|P-value:1.40E-8||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRA1(YLL029W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GAL83(YER027C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GCN3(YKR026C)|FD-Score:6.83|P-value:4.34E-12||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCS1(YDL226C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEF1(YJR040W)|FD-Score:6.67|P-value:1.31E-11||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GET2(YER083C)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GET3(YDL100C)|FD-Score:13.1|P-value:1.71E-39||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GGC1(YDL198C)|FD-Score:-3.79|P-value:7.61E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GPA2(YER020W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPR1(YDL035C)|FD-Score:6.5|P-value:3.96E-11||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GRE3(YHR104W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GSH1(YJL101C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:9.28|P-value:8.11E-21||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOG1(YLR113W)|FD-Score:5.44|P-value:2.70E-8||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:HOM6(YJR139C)|FD-Score:-4.4|P-value:5.40E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HPM1(YIL110W)|FD-Score:-4.81|P-value:7.43E-7||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HRD1(YOL013C)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:ICE2(YIL090W)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IES1(YFL013C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IME4(YGL192W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:INP54(YOL065C)|FD-Score:10.1|P-value:3.97E-24||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins Gene:IPT1(YDR072C)|FD-Score:5.62|P-value:9.46E-9||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRS4(YKR019C)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:IZH2(YOL002C)|FD-Score:10.4|P-value:1.17E-25||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KGD1(YIL125W)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:KHA1(YJL094C)|FD-Score:6.03|P-value:8.05E-10||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:LAP3(YNL239W)|FD-Score:7.55|P-value:2.15E-14||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LHS1(YKL073W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LPD1(YFL018C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MAL33(YBR297W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:MAL-activator protein, part of complex locus MAL3; nonfunctional in genomic reference strain S288C Gene:MCA1(YOR197W)|FD-Score:4.29|P-value:8.77E-6||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MIG3(YER028C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MMT1(YMR177W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MNL2(YLR057W)|FD-Score:6.54|P-value:3.07E-11||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MOT3(YMR070W)|FD-Score:5.15|P-value:1.33E-7||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRH4(YGL064C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRP21(YBL090W)|FD-Score:-5.47|P-value:2.29E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL28(YDR462W)|FD-Score:-3.67|P-value:1.24E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL7(YDR237W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS5(YBR251W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRS3(YJL133W)|FD-Score:-3.25|P-value:5.69E-4||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MRS4(YKR052C)|FD-Score:-5.65|P-value:7.94E-9||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MSM1(YGR171C)|FD-Score:-3.09|P-value:9.86E-4||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MSN1(YOL116W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MSW1(YDR268W)|FD-Score:-4.65|P-value:1.65E-6||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTC2(YKL098W)|FD-Score:5.6|P-value:1.06E-8||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MTC4(YBR255W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:MTF1(YMR228W)|FD-Score:-3.89|P-value:5.06E-5||SGD DESC:Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:MTM1(YGR257C)|FD-Score:-4.85|P-value:6.18E-7||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NEM1(YHR004C)|FD-Score:8.18|P-value:1.41E-16||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NUP170(YBL079W)|FD-Score:5.39|P-value:3.58E-8||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OAF1(YAL051W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OSH3(YHR073W)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OYE2(YHR179W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PCL6(YER059W)|FD-Score:5.3|P-value:5.64E-8||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PEP8(YJL053W)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX29(YDR479C)|FD-Score:4.86|P-value:5.74E-7||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PGD1(YGL025C)|FD-Score:-3.85|P-value:5.82E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO88(YBR106W)|FD-Score:6.07|P-value:6.27E-10||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PHO89(YBR296C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PIM1(YBL022C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PIN2(YOR104W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:PIP2(YOR363C)|FD-Score:6.94|P-value:1.92E-12||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PPM1(YDR435C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRE9(YGR135W)|FD-Score:9.89|P-value:2.26E-23||SGD DESC:Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform Gene:PSP2(YML017W)|FD-Score:4.71|P-value:1.24E-6||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTC1(YDL006W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTC6(YCR079W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:PUB1(YNL016W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:QRI5(YLR204W)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RBG1(YAL036C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RCY1(YJL204C)|FD-Score:7.47|P-value:3.94E-14||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHO5(YNL180C)|FD-Score:5.49|P-value:2.02E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RHR2(YIL053W)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RML2(YEL050C)|FD-Score:-4.91|P-value:4.50E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROY1(YMR258C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL12B(YDR418W)|FD-Score:4.28|P-value:9.37E-6||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:6.89|P-value:2.79E-12||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:18.2|P-value:1.59E-74||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTF1(YGL244W)|FD-Score:-6.43|P-value:6.29E-11||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTK1(YDL025C)|FD-Score:8.58|P-value:4.82E-18||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:RTT10(YPL183C)|FD-Score:-5.44|P-value:2.71E-8||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:RTT106(YNL206C)|FD-Score:4.39|P-value:5.60E-6||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SAC6(YDR129C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAP190(YKR028W)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication Gene:SBH2(YER019C-A)|FD-Score:6.14|P-value:4.05E-10||SGD DESC:Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p Gene:SCP160(YJL080C)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SCS3(YGL126W)|FD-Score:8.15|P-value:1.78E-16||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SEC22(YLR268W)|FD-Score:8.73|P-value:1.22E-18||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEY1(YOR165W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SGF73(YGL066W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SIF2(YBR103W)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SIN3(YOL004W)|FD-Score:3.8|P-value:7.25E-5||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SNF3(YDL194W)|FD-Score:6.49|P-value:4.33E-11||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SOD2(YHR008C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPO7(YAL009W)|FD-Score:7.05|P-value:9.14E-13||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPT23(YKL020C)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SPT3(YDR392W)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRB2(YHR041C)|FD-Score:-4.64|P-value:1.76E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRC1(YML034W)|FD-Score:7.46|P-value:4.27E-14||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SRF1(YDL133W)|FD-Score:6.33|P-value:1.26E-10||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSD1(YDR293C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSM4(YIL030C)|FD-Score:27.5|P-value:6.58E-167||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SSN2(YDR443C)|FD-Score:-5.25|P-value:7.59E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSP120(YLR250W)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SSQ1(YLR369W)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:STE24(YJR117W)|FD-Score:7.05|P-value:9.18E-13||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SVF1(YDR346C)|FD-Score:10.3|P-value:2.54E-25||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SWS2(YNL081C)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TGL3(YMR313C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TLG2(YOL018C)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM7(YNL070W)|FD-Score:4.32|P-value:7.92E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRM9(YML014W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP1(YDR007W)|FD-Score:6.56|P-value:2.68E-11||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.5|P-value:4.11E-11||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:12.4|P-value:8.40E-36||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.22|P-value:2.55E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS65(YGR166W)|FD-Score:6.59|P-value:2.24E-11||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TVP18(YMR071C)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBC7(YMR022W)|FD-Score:19.6|P-value:1.68E-85||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UBP1(YDL122W)|FD-Score:4.97|P-value:3.33E-7||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBP14(YBR058C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:UBP16(YPL072W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UBX4(YMR067C)|FD-Score:7.33|P-value:1.17E-13||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:UGA2(YBR006W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VHR1(YIL056W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication Gene:VPS51(YKR020W)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:4.37|P-value:6.27E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS64(YDR200C)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS74(YDR372C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:YBR226C(YBR226C_d)|FD-Score:6.51|P-value:3.74E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL001W-A(YCL001W-A_p)|FD-Score:9.07|P-value:5.90E-20||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR049C(YCR049C_d)|FD-Score:7.12|P-value:5.24E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR087C-A(YCR087C-A_p)|FD-Score:7.71|P-value:6.50E-15||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL183C(YDL183C)|FD-Score:4.47|P-value:3.95E-6||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR186C(YDR186C)|FD-Score:15.9|P-value:3.26E-57||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR199W(YDR199W_d)|FD-Score:7.58|P-value:1.69E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YDR442W(YDR442W_d)|FD-Score:-4.04|P-value:2.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR537C(YDR537C_d)|FD-Score:7.8|P-value:3.04E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEN1(YER041W)|FD-Score:12.8|P-value:1.36E-37||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER084W(YER084W_p)|FD-Score:15.3|P-value:3.18E-53||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFH7(YFR007W)|FD-Score:5.39|P-value:3.45E-8||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YGL072C(YGL072C_d)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YJL193W(YJL193W_p)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YJR018W(YJR018W_d)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR129C(YJR129C_p)|FD-Score:7.62|P-value:1.26E-14||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YJU3(YKL094W)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLL007C(YLL007C_p)|FD-Score:6.91|P-value:2.41E-12||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR072W(YLR072W_p)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YLR072W is not an esssential gene Gene:YLR111W(YLR111W_d)|FD-Score:5.3|P-value:5.92E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR407W(YLR407W_p)|FD-Score:8.12|P-value:2.32E-16||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YLR422W(YLR422W_p)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YML012C-A(YML012C-A_d)|FD-Score:4.47|P-value:3.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.82|P-value:7.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR057C(YMR057C_d)|FD-Score:4.39|P-value:5.77E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Putative protein of unknown function Gene:YNR005C(YNR005C_d)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOS9(YDR057W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPK1(YKL126W)|FD-Score:5.4|P-value:3.40E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR064W(YPR064W_d)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR153W(YPR153W)|FD-Score:4.76|P-value:9.54E-7||SGD DESC:Putative protein of unknown function Gene:YPT52(YKR014C)|FD-Score:6.84|P-value:3.94E-12||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL148C22.006.80E-1085.24NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YGL116W16.801.42E-632.34CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YPR107C14.401.32E-470.16YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YNL287W14.301.25E-465.18SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YDL126C9.114.17E-200.33CDC48AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
YOL144W8.788.16E-190.09NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YKL058W8.691.80E-180.48TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YHR007C8.211.08E-160.05ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YML105C8.171.60E-160.07SEC65Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
YJL086C_d8.092.91E-160.28YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YNL149C7.822.71E-150.59PGA2Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
YOL127W7.222.57E-130.18RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YIL048W7.049.49E-130.45NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YDL145C6.592.15E-110.27COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YGL093W6.321.28E-100.02SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL030C27.506.58E-167SSM4Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
YMR022W19.601.68E-85UBC7Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
YDL020C18.201.59E-74RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YDR186C15.903.26E-57YDR186CPutative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YER084W_p15.303.18E-53YER084W_pProtein of unknown function; expressed at both mRNA and protein levels
YOR246C15.202.49E-52ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YLR380W13.808.22E-44CSR1Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
YDL100C13.101.71E-39GET3Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
YER041W12.801.36E-37YEN1Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
YKL211C12.408.40E-36TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGL019W11.903.73E-33CKB1Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
YOL002C10.401.17E-25IZH2Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
YDR346C10.302.54E-25SVF1Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance
YFR008W10.201.35E-24FAR7Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
YOL065C10.103.97E-24INP54Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins

GO enrichment analysis for SGTC_1161
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5860SGTC_1030844-0104 51.8 μMChemDiv (Drug-like library)53758040.214286ERAD & cell cycle
0.5290SGTC_384k007-0144 5.2 μMChemDiv (Drug-like library)68248860.290323ERAD & cell cycle
0.5140SGTC_7334069-0007 56.1 μMChemDiv (Drug-like library)57174250.119403ERAD & cell cycle
0.4611.16E-308SGTC_3730844-0104 25.9 μMChemDiv (Drug-like library)53758040.214286ERAD & cell cycle
0.4536.64E-297SGTC_5681193-0097 7.5 μMChemDiv (Drug-like library)36461500.0967742ERAD & cell cycle
0.4434.63E-282SGTC_1026k007-1105 30.6 μMChemDiv (Drug-like library)70667940.298246ERAD & cell cycle
0.3831.41E-205SGTC_352k048-0007 5.1 μMChemDiv (Drug-like library)68119110.119403ERAD & cell cycle
0.3516.19E-171SGTC_8053852-0218 479.0 μMChemDiv (Drug-like library)66153550.0833333ERAD & cell cycle
0.3391.17E-158SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.16129fatty acid desaturase (OLE1)
0.2851.32E-110SGTC_4260988-0037 14.6 μMChemDiv (Drug-like library)53830080.138462ERAD & cell cycle
0.2821.14E-108SGTC_64000l-0018 272.1 μMChemDiv (Drug-like library)68111190.075ubiquinone biosynthesis & proteasome
0.2692.52E-98SGTC_1370438-0332 85.4 μMChemDiv (Drug-like library)20633930.0909091ubiquinone biosynthesis & proteasome
0.2553.03E-88SGTC_1165k072-0275 30.3 μMChemDiv (Drug-like library)61535230.166667
0.2385.97E-77SGTC_23227413319 38.2 μMChembridge (Fragment library)18404000.0606061ERAD & cell cycle
0.2301.01E-71SGTC_1420335-0849 62.7 μMChemDiv (Drug-like library)38890430.0810811ubiquinone biosynthesis & proteasome

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_7171532-024673.2 μM0.476199561992ChemDiv (Drug-like library)185.221842.54512Golgi
SGTC_1025k007-073170 μM0.386256118ChemDiv (Drug-like library)272.2743233.26425
SGTC_553k007-025645.7 μM0.3673476287000ChemDiv (Drug-like library)229.2528832.48414heme biosynthesis & mitochondrial translocase
SGTC_1026k007-110530.6 μM0.2982467066794ChemDiv (Drug-like library)280.152543.60713ERAD & cell cycle
SGTC_14324049-029868.4 μM0.290909741646ChemDiv (Drug-like library)299.2931462.71216
SGTC_384k007-01445.16 μM0.2903236824886ChemDiv (Drug-like library)303.743561.91234ERAD & cell cycle
SGTC_3278913960549.47 μM0.2727277958859Chembridge (Drug-like library)267.6585263.6913
SGTC_20945332512191.67 μM0.259259755774Chembridge (Fragment library)215.247821.59512
SGTC_3054909439749.47 μM0.2586212598354Chembridge (Drug-like library)330.422883.69302fatty acid desaturase (OLE1)
SGTC_7881182-0090188 μM0.255715185ChemDiv (Drug-like library)255.114441.59222endomembrane recycling