aniline yellow

4-phenyldiazenylaniline

A DNA damaging agent.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1169
Screen concentration 25.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 6051
SMILES C1=CC=C(C=C1)N=NC2=CC=C(C=C2)N
Standardized SMILES Nc1ccc(cc1)N=Nc2ccccc2
Molecular weight 197.2358
ALogP 3.45
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.42
% growth inhibition (Hom. pool) 5.89


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6051
Download HIP data (tab-delimited text)  (excel)
Gene:CCT7(YJL111W)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DED81(YHR019C)|FD-Score:-4.12|P-value:1.89E-5|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:ECO1(YFR027W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.02||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG11(YHR007C)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG26(YGL001C)|FD-Score:3.5|P-value:2.30E-4|Clearance:0.23||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FAS1(YKL182W)|FD-Score:-4.16|P-value:1.57E-5|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:PRP4(YPR178W)|FD-Score:4.19|P-value:1.42E-5|Clearance:0.68||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RRP12(YPL012W)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:SOG2(YOR353C)|FD-Score:-3.73|P-value:9.61E-5|Clearance:0||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:VAS1(YGR094W)|FD-Score:3.25|P-value:5.86E-4|Clearance:0.19||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YLR140W(YLR140W_d)|FD-Score:-3.32|P-value:4.47E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:CCT7(YJL111W)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DED81(YHR019C)|FD-Score:-4.12|P-value:1.89E-5|Clearance:0||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:ECO1(YFR027W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.02||SGD DESC:Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG11(YHR007C)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG26(YGL001C)|FD-Score:3.5|P-value:2.30E-4|Clearance:0.23||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:FAS1(YKL182W)|FD-Score:-4.16|P-value:1.57E-5|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:PRP4(YPR178W)|FD-Score:4.19|P-value:1.42E-5|Clearance:0.68||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RRP12(YPL012W)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; contains HEAT-repeats Gene:SOG2(YOR353C)|FD-Score:-3.73|P-value:9.61E-5|Clearance:0||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:VAS1(YGR094W)|FD-Score:3.25|P-value:5.86E-4|Clearance:0.19||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YLR140W(YLR140W_d)|FD-Score:-3.32|P-value:4.47E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6051
Download HOP data (tab-delimited text)  (excel)
Gene:AAT1(YKL106W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:ATP23(YNR020C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BIO2(YGR286C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BLM10(YFL007W)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:CLB4(YLR210W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:HOR2(YER062C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication Gene:HRT3(YLR097C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:JLP1(YLL057C)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:MDM32(YOR147W)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MMT1(YMR177W)|FD-Score:-3.72|P-value:9.85E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MSS11(YMR164C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:OCA1(YNL099C)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PEP4(YPL154C)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PEX6(YNL329C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:RPL26B(YGR034W)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPN14(YGL004C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:RPN4(YDL020C)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC4(YNL254C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SCW11(YGL028C)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SGE1(YPR198W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:STB6(YKL072W)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:TOM70(YNL121C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPC1(YGR096W)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:ULS1(YOR191W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VHC1(YBR235W_p)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VPS61(YDR136C_d)|FD-Score:4.77|P-value:9.36E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR232C(YBR232C_d)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function Gene:YGR151C(YGR151C_d)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHL044W(YHL044W_p)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIL029C(YIL029C_p)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJR037W(YJR037W_d)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKL044W(YKL044W_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-4.56|P-value:2.57E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YKL102C(YKL102C_d)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YKR032W(YKR032W_d)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR118C(YLR118C)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YMR034C(YMR034C_p)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR279C(YMR279C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YOP1(YPR028W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YPL168W(YPL168W_p)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPL245W(YPL245W_p)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR195C(YPR195C_d)|FD-Score:4.83|P-value:6.85E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YTA7(YGR270W)|FD-Score:-3.67|P-value:1.24E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:AAT1(YKL106W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:ATP23(YNR020C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BIO2(YGR286C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BLM10(YFL007W)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:CLB4(YLR210W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:HOR2(YER062C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication Gene:HRT3(YLR097C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:JLP1(YLL057C)|FD-Score:3.81|P-value:7.01E-5||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:MDM32(YOR147W)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34 Gene:MMT1(YMR177W)|FD-Score:-3.72|P-value:9.85E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MSS11(YMR164C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:OCA1(YNL099C)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:PEP4(YPL154C)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PEX6(YNL329C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:RPL26B(YGR034W)|FD-Score:-3.94|P-value:4.14E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPN14(YGL004C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:RPN4(YDL020C)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC4(YNL254C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SCW11(YGL028C)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SGE1(YPR198W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:STB6(YKL072W)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:TOM70(YNL121C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPC1(YGR096W)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:ULS1(YOR191W)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VHC1(YBR235W_p)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VPS61(YDR136C_d)|FD-Score:4.77|P-value:9.36E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR232C(YBR232C_d)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative protein of unknown function Gene:YGR151C(YGR151C_d)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHL044W(YHL044W_p)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIL029C(YIL029C_p)|FD-Score:3.72|P-value:9.99E-5||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJR037W(YJR037W_d)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKL044W(YKL044W_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:-4.56|P-value:2.57E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YKL102C(YKL102C_d)|FD-Score:-3.6|P-value:1.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YKR032W(YKR032W_d)|FD-Score:-3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR118C(YLR118C)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YMR034C(YMR034C_p)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR279C(YMR279C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication Gene:YOP1(YPR028W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YPL168W(YPL168W_p)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPL245W(YPL245W_p)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR195C(YPR195C_d)|FD-Score:4.83|P-value:6.85E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YTA7(YGR270W)|FD-Score:-3.67|P-value:1.24E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR178W4.191.42E-50.68PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YGL001C3.502.30E-40.23ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YFR027W3.285.24E-40.02ECO1Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
YHR007C3.255.72E-40.01ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YGR094W3.255.86E-40.19VAS1Mitochondrial and cytoplasmic valyl-tRNA synthetase
YLR272C3.050.001132.63E-4YCS4Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YNL306W3.050.001130.05MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
YGR119C3.010.001330.05NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YOR210W2.950.001590.06RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YLR212C2.890.001910.09TUB4Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
YOR149C2.810.002510.02SMP3Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
YKL049C2.780.002700.06CSE4Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions
YDR023W2.730.003200.05SES1Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
YPL175W2.680.003690.01SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YNL261W2.670.003800.02ORC5Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR195C_d4.836.85E-7YPR195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR136C_d4.779.36E-7VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YNL099C4.493.48E-6OCA1Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA
YKL072W4.483.75E-6STB6Protein that binds Sin3p in a two-hybrid assay
YPL245W_p4.356.94E-6YPL245W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
YMR279C4.231.19E-5YMR279CPutative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication
YOR147W3.983.42E-5MDM32Mitochondrial inner membrane protein with similarity to Mdm31p, required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34
YBR235W_p3.983.44E-5VHC1_pVacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family
YOR191W3.953.98E-5ULS1Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
YLR118C3.875.45E-5YLR118CAcyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS
YDL020C3.865.59E-5RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YLL057C3.817.01E-5JLP1Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation
YGR151C_d3.768.39E-5YGR151C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress
YFL007W3.768.43E-5BLM10Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200
YPL154C3.739.62E-5PEP4Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates

GO enrichment analysis for SGTC_1169
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0985.92E-14SGTC_28147977482 43.0 μMChembridge (Drug-like library)64567620.0689655
0.0849.56E-11SGTC_24435493264 126.7 μMMiscellaneous53400550.125
0.0841.12E-10SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.179487
0.0741.19E-8SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.175
0.0723.48E-8SGTC_2051486-1330 71.9 μMChemDiv (Drug-like library)41188340.05
0.0715.51E-8SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.25
0.0699.93E-8SGTC_1717st033143 58.0 μMTimTec (Natural product derivative library)6746160.0545455
0.0681.63E-7SGTC_24405628896 157.8 μMMiscellaneous55220480.125
0.0681.94E-7SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.0892857
0.0681.96E-7SGTC_1705st027868 56.3 μMTimTec (Natural product derivative library)2929480.121951
0.0672.53E-7SGTC_23975161689 200.0 μMMiscellaneous22599420.115385
0.0663.68E-7SGTC_11760848-0131 83.3 μMChemDiv (Drug-like library)67690400.122807Golgi
0.0664.02E-7SGTC_222nsc-310342 10.7 μMMiscellaneous4325560.08
0.0664.71E-7SGTC_1026k007-1105 30.6 μMChemDiv (Drug-like library)70667940.1ERAD & cell cycle
0.0654.77E-7SGTC_1994063-0024 41.6 μMChemDiv (Drug-like library)3062150.0625

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12330325-040814.2 μM0.60869612874ChemDiv (Drug-like library)211.262423.93813
SGTC_8550428-002025 μM0.3939396812414ChemDiv (Drug-like library)226.23072.15914Golgi
SGTC_8690564-008044.1 μM0.3611116843321ChemDiv (Drug-like library)317.34133.93125
SGTC_7690302-023176 μM0.34375571869ChemDiv (Drug-like library)225.2894.42513
SGTC_6690108-002126.2 μM0.31034517201ChemDiv (Drug-like library)292.331843.45724fatty acid desaturase (OLE1)
SGTC_2236dapsone200 μM0.3076922955Miscellaneous248.300881.43924
SGTC_250118-022335.66 μM0.2926835333628ChemDiv (Drug-like library)294.307960.9225
SGTC_6140462-0418107 μM0.2894745188303ChemDiv (Drug-like library)256.303043.17625
SGTC_930109-004559.23 μM0.28571488733ChemDiv (Drug-like library)210.23132.53413DNA damage response
SGTC_9052-aminofluorene215 μM0.281251539ChemDiv (Drug-like library)181.233142.74611cell wall signaling