lucigenin

10-methyl-9-(10-methylacridin-10-ium-9-yl)acridin-10-ium

An acridine derivative; a dye stuff and potential carcinogen.

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PubChem MeSH terms: Luminescent Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_117
Screen concentration 22.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 65100
SMILES C[N+]1=C2C=CC=CC2=C(C3=CC=CC=C31)C4=C5C=CC=CC5=[N+](C6=CC=CC=C64)C
Standardized SMILES C[n+]1c2ccccc2c(c3c4ccccc4[n+](C)c5ccccc35)c6ccccc16
Molecular weight 386.4877
ALogP 7.36
H-bond donor count 0
H-bond acceptor count 0
Response signature redox potentiating

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.71
% growth inhibition (Hom. pool) 9.97


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 65100
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:4.71|P-value:1.23E-6|Clearance:0.85||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CBK1(YNL161W)|FD-Score:3.86|P-value:5.67E-5|Clearance:0.17||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CMD1(YBR109C)|FD-Score:5.79|P-value:3.48E-9|Clearance:0.07||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:GLC7(YER133W)|FD-Score:4.96|P-value:3.50E-7|Clearance:0.25||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HSH49(YOR319W)|FD-Score:-4.11|P-value:1.94E-5|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSG1(YGL099W)|FD-Score:11.9|P-value:6.35E-33|Clearance:3.83||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:8.06|P-value:3.71E-16|Clearance:0.57||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP58(YOR310C)|FD-Score:-4.72|P-value:1.18E-6|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUF2(YOL069W)|FD-Score:6.17|P-value:3.52E-10|Clearance:0.26||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:PUP1(YOR157C)|FD-Score:-3.48|P-value:2.50E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:SEC13(YLR208W)|FD-Score:5.91|P-value:1.76E-9|Clearance:0.11||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TAF12(YDR145W)|FD-Score:-3.81|P-value:6.84E-5|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TOA2(YKL058W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.34||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TUB1(YML085C)|FD-Score:7.49|P-value:3.46E-14|Clearance:1.32||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDR526C(YDR526C_d)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL114C(YNL114C_d)|FD-Score:-3.1|P-value:9.59E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPD1(YDL235C)|FD-Score:3.15|P-value:8.12E-4|Clearance:0.22||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YTH1(YPR107C)|FD-Score:5.73|P-value:5.14E-9|Clearance:0.76||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ABF1(YKL112W)|FD-Score:4.71|P-value:1.23E-6|Clearance:0.85||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CBK1(YNL161W)|FD-Score:3.86|P-value:5.67E-5|Clearance:0.17||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CMD1(YBR109C)|FD-Score:5.79|P-value:3.48E-9|Clearance:0.07||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:GLC7(YER133W)|FD-Score:4.96|P-value:3.50E-7|Clearance:0.25||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HSH49(YOR319W)|FD-Score:-4.11|P-value:1.94E-5|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSG1(YGL099W)|FD-Score:11.9|P-value:6.35E-33|Clearance:3.83||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:8.06|P-value:3.71E-16|Clearance:0.57||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP58(YOR310C)|FD-Score:-4.72|P-value:1.18E-6|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUF2(YOL069W)|FD-Score:6.17|P-value:3.52E-10|Clearance:0.26||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:PUP1(YOR157C)|FD-Score:-3.48|P-value:2.50E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:SEC13(YLR208W)|FD-Score:5.91|P-value:1.76E-9|Clearance:0.11||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TAF12(YDR145W)|FD-Score:-3.81|P-value:6.84E-5|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TOA2(YKL058W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.34||SGD DESC:TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress Gene:TUB1(YML085C)|FD-Score:7.49|P-value:3.46E-14|Clearance:1.32||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDR526C(YDR526C_d)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL114C(YNL114C_d)|FD-Score:-3.1|P-value:9.59E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPD1(YDL235C)|FD-Score:3.15|P-value:8.12E-4|Clearance:0.22||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YTH1(YPR107C)|FD-Score:5.73|P-value:5.14E-9|Clearance:0.76||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 65100
Download HOP data (tab-delimited text)  (excel)
Gene:APJ1(YNL077W)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:APL6(YGR261C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM3(YBR288C)|FD-Score:4.36|P-value:6.60E-6||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ARF1(YDL192W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARG82(YDR173C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:BCK2(YER167W)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BER1(YLR412W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BFR1(YOR198C)|FD-Score:4.87|P-value:5.53E-7||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BSC5(YNR069C)|FD-Score:4.27|P-value:9.93E-6||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BUD16(YEL029C)|FD-Score:-6.07|P-value:6.49E-10||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD31(YCR063W)|FD-Score:6.85|P-value:3.78E-12||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:BUL1(YMR275C)|FD-Score:-4.29|P-value:8.83E-6||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAP1(YKL007W)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCC2(YDR270W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CDC73(YLR418C)|FD-Score:8.55|P-value:5.92E-18||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CHO2(YGR157W)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CHS5(YLR330W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CKA2(YOR061W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLD1(YGR110W)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CNB1(YKL190W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CTK3(YML112W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCG1(YIR030C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DHH1(YDL160C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DID4(YKL002W)|FD-Score:4.77|P-value:9.42E-7||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DYN3(YMR299C)|FD-Score:5.37|P-value:3.85E-8||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EAF7(YNL136W)|FD-Score:5.68|P-value:6.75E-9||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ELM1(YKL048C)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG2(YMR202W)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FET3(YMR058W)|FD-Score:7.34|P-value:1.05E-13||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:FRE5(YOR384W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCS1(YDL226C)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDA1(YEL042W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GIM4(YEL003W)|FD-Score:16.7|P-value:7.94E-63||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GIP3(YPL137C)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GLN3(YER040W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GPA2(YER020W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPB2(YAL056W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTR1(YML121W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HIR3(YJR140C)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HPC2(YBR215W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:IBA57(YJR122W)|FD-Score:7.89|P-value:1.45E-15||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IDH2(YOR136W)|FD-Score:6.33|P-value:1.26E-10||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IME4(YGL192W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IMP2'(YIL154C)|FD-Score:13.1|P-value:1.97E-39||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP53(YOR109W)|FD-Score:3.73|P-value:9.53E-5||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:ISA1(YLL027W)|FD-Score:7.07|P-value:7.86E-13||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:ISA2(YPR067W)|FD-Score:7.46|P-value:4.45E-14||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:JIP4(YDR475C)|FD-Score:4.28|P-value:9.21E-6||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KAP104(YBR017C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:KAR5(YMR065W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KRE2(YDR483W)|FD-Score:11|P-value:1.94E-28||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LEO1(YOR123C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:MAL13(YGR288W)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MCA1(YOR197W)|FD-Score:5.31|P-value:5.41E-8||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MON1(YGL124C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRP49(YKL167C)|FD-Score:4.82|P-value:7.28E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL40(YPL173W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB2(YGR014W)|FD-Score:-4.05|P-value:2.60E-5||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSH2(YOL090W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NBP2(YDR162C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NHX1(YDR456W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NUP170(YBL079W)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI6(YDL096C_d)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:OPI7(YDR360W_d)|FD-Score:6.82|P-value:4.71E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OST3(YOR085W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PAC10(YGR078C)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PEP12(YOR036W)|FD-Score:7.59|P-value:1.55E-14||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:5.08|P-value:1.90E-7||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:5.46|P-value:2.44E-8||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX17(YNL214W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PHO84(YML123C)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PKH3(YDR466W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMT1(YDL095W)|FD-Score:9.16|P-value:2.65E-20||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PMT2(YAL023C)|FD-Score:4.93|P-value:4.07E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:POS5(YPL188W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PTC1(YDL006W)|FD-Score:6.53|P-value:3.18E-11||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTK2(YJR059W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUF3(YLL013C)|FD-Score:8.59|P-value:4.54E-18||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD18(YCR066W)|FD-Score:5.78|P-value:3.82E-9||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RFM1(YOR279C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGT1(YKL038W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RMD1(YDL001W)|FD-Score:5.94|P-value:1.43E-9||SGD DESC:Cytoplasmic protein required for sporulation Gene:RMD5(YDR255C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:RML2(YEL050C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROM2(YLR371W)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL20B(YOR312C)|FD-Score:5.62|P-value:9.28E-9||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL38(YLR325C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RTT103(YDR289C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC1(YKL212W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC7(YDR389W)|FD-Score:9.15|P-value:2.95E-20||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAS4(YDR181C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SAT4(YCR008W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SDH1(YKL148C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEA4(YBL104C)|FD-Score:5.38|P-value:3.79E-8||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SIS2(YKR072C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKY1(YMR216C)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLC1(YDL052C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SLG1(YOR008C)|FD-Score:-4.63|P-value:1.85E-6||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNF4(YGL115W)|FD-Score:-4.51|P-value:3.21E-6||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNF7(YLR025W)|FD-Score:11.2|P-value:3.47E-29||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:5.54|P-value:1.51E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNU66(YOR308C)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SOD2(YHR008C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPS18(YNL204C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPT7(YBR081C)|FD-Score:-3.8|P-value:7.16E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SRY1(YKL218C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSZ1(YHR064C)|FD-Score:4.27|P-value:9.73E-6||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:STP22(YCL008C)|FD-Score:5.42|P-value:3.01E-8||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SVP26(YHR181W)|FD-Score:7.99|P-value:6.95E-16||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWA2(YDR320C)|FD-Score:6.14|P-value:4.18E-10||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWI4(YER111C)|FD-Score:5.46|P-value:2.32E-8||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TAT1(YBR069C)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDH1(YJL052W)|FD-Score:7.73|P-value:5.21E-15||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TOM71(YHR117W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:-3.97|P-value:3.66E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRK1(YJL129C)|FD-Score:6.15|P-value:3.96E-10||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRS65(YGR166W)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:UBA3(YPR066W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:URE2(YNL229C)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAM10(YOR068C)|FD-Score:7.36|P-value:8.97E-14||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VAM6(YDL077C)|FD-Score:9.09|P-value:5.06E-20||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VAM7(YGL212W)|FD-Score:7.69|P-value:7.35E-15||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VEL1(YGL258W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS1(YKR001C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:7.6|P-value:1.52E-14||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS21(YOR089C)|FD-Score:7.02|P-value:1.10E-12||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS24(YKL041W)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:8.9|P-value:2.84E-19||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:10.5|P-value:3.03E-26||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS3(YDR495C)|FD-Score:7.74|P-value:5.17E-15||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS35(YJL154C)|FD-Score:5.65|P-value:7.95E-9||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS36(YLR417W)|FD-Score:17|P-value:3.37E-65||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS4(YPR173C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:5.34|P-value:4.64E-8||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS5(YOR069W)|FD-Score:7.43|P-value:5.24E-14||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS60(YDR486C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS74(YDR372C)|FD-Score:7.06|P-value:8.17E-13||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:VPS8(YAL002W)|FD-Score:6.43|P-value:6.26E-11||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR028W(YAR028W_p)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBR224W(YBR224W_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 Gene:YBR238C(YBR238C)|FD-Score:9.33|P-value:5.47E-21||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR277C(YBR277C_d)|FD-Score:-3.81|P-value:6.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCL001W-A(YCL001W-A_p)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR064C(YCR064C_d)|FD-Score:10.5|P-value:6.17E-26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR271C(YDR271C_d)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR290W(YDR290W_d)|FD-Score:7.61|P-value:1.32E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR433W(YDR433W_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR442W(YDR442W_d)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:11.4|P-value:3.43E-30||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR467C(YDR467C_d)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER181C(YER181C_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YGL217C(YGL217C_d)|FD-Score:-3.99|P-value:3.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGR169C-A(YGR169C-A_p)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHR140W(YHR140W_p)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Putative integral membrane protein of unknown function Gene:YKE2(YLR200W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL023W(YKL023W_p)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLL044W(YLL044W_d)|FD-Score:5.39|P-value:3.54E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLL058W(YLL058W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLL059C(YLL059C_d)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR108C(YLR108C_p)|FD-Score:6.67|P-value:1.32E-11||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YLR171W(YLR171W_d)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR402W(YLR402W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML009W-B(YML009W-B_d)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR155W(YMR155W_p)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YOR1(YGR281W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPK1(YKL126W)|FD-Score:4.92|P-value:4.22E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL034W(YPL034W_p)|FD-Score:5.21|P-value:9.54E-8||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPR039W(YPR039W_d)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR123C(YPR123C_d)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT10(YBR264C)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:YTP1(YNL237W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZUO1(YGR285C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:APJ1(YNL077W)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:APL6(YGR261C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM3(YBR288C)|FD-Score:4.36|P-value:6.60E-6||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:ARF1(YDL192W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARG82(YDR173C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:BCK2(YER167W)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:BER1(YLR412W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BFR1(YOR198C)|FD-Score:4.87|P-value:5.53E-7||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BSC5(YNR069C)|FD-Score:4.27|P-value:9.93E-6||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BUD16(YEL029C)|FD-Score:-6.07|P-value:6.49E-10||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:BUD31(YCR063W)|FD-Score:6.85|P-value:3.78E-12||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:BUL1(YMR275C)|FD-Score:-4.29|P-value:8.83E-6||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAP1(YKL007W)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCC2(YDR270W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CDC73(YLR418C)|FD-Score:8.55|P-value:5.92E-18||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CHO2(YGR157W)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CHS5(YLR330W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CKA2(YOR061W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLD1(YGR110W)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CNB1(YKL190W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CTK3(YML112W)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCG1(YIR030C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DHH1(YDL160C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DID4(YKL002W)|FD-Score:4.77|P-value:9.42E-7||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DYN3(YMR299C)|FD-Score:5.37|P-value:3.85E-8||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EAF7(YNL136W)|FD-Score:5.68|P-value:6.75E-9||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ELM1(YKL048C)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG2(YMR202W)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FET3(YMR058W)|FD-Score:7.34|P-value:1.05E-13||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:FRE5(YOR384W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCS1(YDL226C)|FD-Score:3.84|P-value:6.13E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDA1(YEL042W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GIM4(YEL003W)|FD-Score:16.7|P-value:7.94E-63||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GIP3(YPL137C)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GLN3(YER040W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GPA2(YER020W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPB2(YAL056W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GRR1(YJR090C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTR1(YML121W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HIR3(YJR140C)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HPC2(YBR215W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:IBA57(YJR122W)|FD-Score:7.89|P-value:1.45E-15||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IDH2(YOR136W)|FD-Score:6.33|P-value:1.26E-10||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IME4(YGL192W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IMP2'(YIL154C)|FD-Score:13.1|P-value:1.97E-39||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP53(YOR109W)|FD-Score:3.73|P-value:9.53E-5||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:ISA1(YLL027W)|FD-Score:7.07|P-value:7.86E-13||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:ISA2(YPR067W)|FD-Score:7.46|P-value:4.45E-14||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:JIP4(YDR475C)|FD-Score:4.28|P-value:9.21E-6||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KAP104(YBR017C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:KAR5(YMR065W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KRE2(YDR483W)|FD-Score:11|P-value:1.94E-28||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LEO1(YOR123C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:MAL13(YGR288W)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MCA1(YOR197W)|FD-Score:5.31|P-value:5.41E-8||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MON1(YGL124C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRP49(YKL167C)|FD-Score:4.82|P-value:7.28E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL40(YPL173W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB2(YGR014W)|FD-Score:-4.05|P-value:2.60E-5||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSH2(YOL090W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:NBP2(YDR162C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NHX1(YDR456W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NUP170(YBL079W)|FD-Score:-3.79|P-value:7.57E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI6(YDL096C_d)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:OPI7(YDR360W_d)|FD-Score:6.82|P-value:4.71E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OST3(YOR085W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PAC10(YGR078C)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PEP12(YOR036W)|FD-Score:7.59|P-value:1.55E-14||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:5.08|P-value:1.90E-7||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:5.46|P-value:2.44E-8||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX17(YNL214W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PHO84(YML123C)|FD-Score:-3.81|P-value:7.02E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PKH3(YDR466W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMT1(YDL095W)|FD-Score:9.16|P-value:2.65E-20||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PMT2(YAL023C)|FD-Score:4.93|P-value:4.07E-7||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:POS5(YPL188W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PTC1(YDL006W)|FD-Score:6.53|P-value:3.18E-11||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTK2(YJR059W)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUF3(YLL013C)|FD-Score:8.59|P-value:4.54E-18||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD18(YCR066W)|FD-Score:5.78|P-value:3.82E-9||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RFM1(YOR279C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGT1(YKL038W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RMD1(YDL001W)|FD-Score:5.94|P-value:1.43E-9||SGD DESC:Cytoplasmic protein required for sporulation Gene:RMD5(YDR255C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:RML2(YEL050C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROM2(YLR371W)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL20B(YOR312C)|FD-Score:5.62|P-value:9.28E-9||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL38(YLR325C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RTT103(YDR289C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC1(YKL212W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC7(YDR389W)|FD-Score:9.15|P-value:2.95E-20||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAS4(YDR181C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SAT4(YCR008W)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SDH1(YKL148C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEA4(YBL104C)|FD-Score:5.38|P-value:3.79E-8||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SIS2(YKR072C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKY1(YMR216C)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLC1(YDL052C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SLG1(YOR008C)|FD-Score:-4.63|P-value:1.85E-6||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNF4(YGL115W)|FD-Score:-4.51|P-value:3.21E-6||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:SNF7(YLR025W)|FD-Score:11.2|P-value:3.47E-29||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:5.54|P-value:1.51E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SNU66(YOR308C)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SOD2(YHR008C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPS18(YNL204C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPT7(YBR081C)|FD-Score:-3.8|P-value:7.16E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SRY1(YKL218C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSZ1(YHR064C)|FD-Score:4.27|P-value:9.73E-6||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:STP22(YCL008C)|FD-Score:5.42|P-value:3.01E-8||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SVP26(YHR181W)|FD-Score:7.99|P-value:6.95E-16||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWA2(YDR320C)|FD-Score:6.14|P-value:4.18E-10||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWI4(YER111C)|FD-Score:5.46|P-value:2.32E-8||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TAT1(YBR069C)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDH1(YJL052W)|FD-Score:7.73|P-value:5.21E-15||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TOM71(YHR117W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:-3.97|P-value:3.66E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRK1(YJL129C)|FD-Score:6.15|P-value:3.96E-10||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRS65(YGR166W)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:UBA3(YPR066W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:URE2(YNL229C)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAM10(YOR068C)|FD-Score:7.36|P-value:8.97E-14||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VAM6(YDL077C)|FD-Score:9.09|P-value:5.06E-20||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VAM7(YGL212W)|FD-Score:7.69|P-value:7.35E-15||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VEL1(YGL258W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C Gene:VPS1(YKR001C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:7.6|P-value:1.52E-14||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS21(YOR089C)|FD-Score:7.02|P-value:1.10E-12||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS24(YKL041W)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:8.9|P-value:2.84E-19||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:10.5|P-value:3.03E-26||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS3(YDR495C)|FD-Score:7.74|P-value:5.17E-15||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS35(YJL154C)|FD-Score:5.65|P-value:7.95E-9||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS36(YLR417W)|FD-Score:17|P-value:3.37E-65||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS4(YPR173C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:5.34|P-value:4.64E-8||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS5(YOR069W)|FD-Score:7.43|P-value:5.24E-14||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS60(YDR486C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS74(YDR372C)|FD-Score:7.06|P-value:8.17E-13||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:VPS8(YAL002W)|FD-Score:6.43|P-value:6.26E-11||SGD DESC:Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.8|P-value:7.29E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR028W(YAR028W_p)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBR224W(YBR224W_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 Gene:YBR238C(YBR238C)|FD-Score:9.33|P-value:5.47E-21||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR277C(YBR277C_d)|FD-Score:-3.81|P-value:6.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCL001W-A(YCL001W-A_p)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR064C(YCR064C_d)|FD-Score:10.5|P-value:6.17E-26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR271C(YDR271C_d)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR290W(YDR290W_d)|FD-Score:7.61|P-value:1.32E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR433W(YDR433W_d)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR442W(YDR442W_d)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:11.4|P-value:3.43E-30||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YDR467C(YDR467C_d)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER181C(YER181C_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YGL217C(YGL217C_d)|FD-Score:-3.99|P-value:3.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGR169C-A(YGR169C-A_p)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHR140W(YHR140W_p)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Putative integral membrane protein of unknown function Gene:YKE2(YLR200W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL023W(YKL023W_p)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLL044W(YLL044W_d)|FD-Score:5.39|P-value:3.54E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant Gene:YLL058W(YLL058W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLL059C(YLL059C_d)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR108C(YLR108C_p)|FD-Score:6.67|P-value:1.32E-11||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YLR171W(YLR171W_d)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR402W(YLR402W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML009W-B(YML009W-B_d)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR155W(YMR155W_p)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YOR1(YGR281W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPK1(YKL126W)|FD-Score:4.92|P-value:4.22E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL034W(YPL034W_p)|FD-Score:5.21|P-value:9.54E-8||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPR039W(YPR039W_d)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR123C(YPR123C_d)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT10(YBR264C)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:YTP1(YNL237W)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZUO1(YGR285C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W11.906.35E-333.83LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W8.063.71E-160.57NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YML085C7.493.46E-141.32TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YOL069W6.173.52E-100.26NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YLR208W5.911.76E-90.11SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YBR109C5.793.48E-90.07CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YPR107C5.735.14E-90.77YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YER133W4.963.50E-70.25GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YKL112W4.711.23E-60.85ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YNL161W3.865.67E-50.17CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YKL058W3.691.11E-40.34TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YDR526C_d3.363.94E-40.21YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL235C3.158.12E-40.22YPD1Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
YDR081C2.930.001700.03PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YOR046C2.900.001860.14DBP5Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR417W17.003.37E-65VPS36Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
YEL003W16.707.94E-63GIM4Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
YIL154C13.101.97E-39IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YDR455C_d11.403.43E-30YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YLR025W11.203.47E-29SNF7One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
YDR483W11.001.94E-28KRE2Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
YPL065W10.503.03E-26VPS28Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
YCR064C_d10.506.17E-26YCR064C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
YBR238C9.335.47E-21YBR238CMitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span
YDL095W9.162.65E-20PMT1Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
YDR389W9.152.95E-20SAC7GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
YDL077C9.095.06E-20VAM6Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
YJR102C8.902.84E-19VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YLL013C8.594.54E-18PUF3Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
YLR418C8.555.92E-18CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_117
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2665.62E-96SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.12NEO1
0.2594.89E-91SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 54.9 μMTimTec (Pure natural product library)4428820.107143redox potentiating
0.2183.24E-64SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0189873Golgi
0.2178.50E-64SGTC_14954469-0029 237.0 μMChemDiv (Drug-like library)7936800.0754717
0.2073.66E-58SGTC_2064546-0025 72.0 μMChemDiv (Drug-like library)66207450.105263NEO1
0.2075.80E-58SGTC_5283492-0019 172.0 μMChemDiv (Drug-like library)28692620.113208calcium & mitochondrial duress
0.2034.52E-56SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.0625
0.1951.25E-51SGTC_232clozapine 119.0 μMMiscellaneous28180.113208NEO1
0.1911.76E-49SGTC_502l-744,832 89.3 μMICCB bioactive library66103540.0769231calcium & mitochondrial duress
0.1912.30E-49SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0882353
0.1885.72E-48SGTC_1803st050068 52.4 μMTimTec (Natural product derivative library)39840590.0689655NEO1-PIK1
0.1851.82E-46SGTC_748act1.228 77.9 μMChemDiv (Drug-like library)44215050.0512821redox potentiating
0.1852.09E-46SGTC_459nigericin 15.3 μMICCB bioactive library342300.0102041
0.1751.34E-41SGTC_400oxethazaine 37.5 μMMiscellaneous46210.125calcium & mitochondrial duress
0.1661.73E-37SGTC_6704204-0025 119.0 μMChemDiv (Drug-like library)7190390.115385copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_230MPP+141.67 μM0.29629639484Miscellaneous170.230382.95300
SGTC_2674-Nitroquinoline-1-oxide (4-NQO)44.4 nM0.2857145955Miscellaneous190.155541.06603DNA damage response
SGTC_2591skatole100 μM0.2666676736Microsource (Natural product library)131.174462.60910
SGTC_6470140-023681 μM0.242424990837ChemDiv (Drug-like library)406.606724.07104
SGTC_2991273-00591.44 μM0.2252848077ChemDiv (Drug-like library)357.595749.5600
SGTC_452diindolylmethane8 μM0.218753071ICCB bioactive library246.306464.39120RNA processing & uracil transport
SGTC_1516st013856110 μM0.2162165281404TimTec (Pure natural product library)182.22122.45511
SGTC_22577960413200 μM0.2162162974689Chembridge (Fragment library)237.725242.53122NEO1-PIK1
SGTC_1070358-00222.69 μM0.212121521106ChemDiv (Drug-like library)350.453965.87124SWF1 & branched chain AA biosynthesis
SGTC_1731273-00612.69 μM0.2093022847414ChemDiv (Drug-like library)419.6651211.13600mitochondrial stress