0368-0077

pyridin-3-yl 3,4-dichlorobenzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1171
Screen concentration 21.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 3239779
SMILES C1=CC(=CN=C1)OC(=O)C2=CC(=C(C=C2)Cl)Cl
Standardized SMILES Clc1ccc(cc1Cl)C(=O)Oc2cccnc2
Molecular weight 268.0955
ALogP 3.44
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.01
% growth inhibition (Hom. pool) 7.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3239779
Download HIP data (tab-delimited text)  (excel)
Gene:ALG7(YBR243C)|FD-Score:-3.38|P-value:3.60E-4|Clearance:0||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:CCT3(YJL014W)|FD-Score:5.51|P-value:1.75E-8|Clearance:0.4||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:3.41|P-value:3.21E-4|Clearance:0.12||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CEP3(YMR168C)|FD-Score:3.97|P-value:3.62E-5|Clearance:0.09||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:DIB1(YPR082C)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.01||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:DOP1(YDR141C)|FD-Score:4.27|P-value:9.63E-6|Clearance:0.14||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERG26(YGL001C)|FD-Score:-3.37|P-value:3.80E-4|Clearance:0||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:ILV5(YLR355C)|FD-Score:3.21|P-value:6.54E-4|Clearance:0.01||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MOB1(YIL106W)|FD-Score:3.3|P-value:4.88E-4|Clearance:0.05||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MRS6(YOR370C)|FD-Score:3.8|P-value:7.31E-5|Clearance:0.02||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:PKC1(YBL105C)|FD-Score:5.73|P-value:4.99E-9|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE2(YPR103W)|FD-Score:-3.44|P-value:2.89E-4|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RIM2(YBR192W)|FD-Score:5.56|P-value:1.36E-8|Clearance:0.04||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPA43(YOR340C)|FD-Score:3.5|P-value:2.34E-4|Clearance:0.08||SGD DESC:RNA polymerase I subunit A43 Gene:RPP1(YHR062C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT6(YGL048C)|FD-Score:3.2|P-value:6.89E-4|Clearance:0.05||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRS1(YOR294W)|FD-Score:3.12|P-value:8.93E-4|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC58(YLR033W)|FD-Score:6.48|P-value:4.48E-11|Clearance:0.75||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.13|P-value:1.82E-5|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:5.11|P-value:1.60E-7|Clearance:0.59||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC10(YLR166C)|FD-Score:3.15|P-value:8.24E-4|Clearance:0.01||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEC27(YGL137W)|FD-Score:3.78|P-value:7.85E-5|Clearance:0.08||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SEN1(YLR430W)|FD-Score:3.11|P-value:9.46E-4|Clearance:0.05||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SFH1(YLR321C)|FD-Score:3.88|P-value:5.22E-5|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SLN1(YIL147C)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.04||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SPC105(YGL093W)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.01||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:STS1(YIR011C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.19||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:YDL196W(YDL196W_d)|FD-Score:4.52|P-value:3.11E-6|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:5.73|P-value:5.00E-9|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YTH1(YPR107C)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ALG7(YBR243C)|FD-Score:-3.38|P-value:3.60E-4|Clearance:0||SGD DESC:UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin Gene:CCT3(YJL014W)|FD-Score:5.51|P-value:1.75E-8|Clearance:0.4||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:3.41|P-value:3.21E-4|Clearance:0.12||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CEP3(YMR168C)|FD-Score:3.97|P-value:3.62E-5|Clearance:0.09||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:DIB1(YPR082C)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.01||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:DOP1(YDR141C)|FD-Score:4.27|P-value:9.63E-6|Clearance:0.14||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:ERG26(YGL001C)|FD-Score:-3.37|P-value:3.80E-4|Clearance:0||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:ILV5(YLR355C)|FD-Score:3.21|P-value:6.54E-4|Clearance:0.01||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:MOB1(YIL106W)|FD-Score:3.3|P-value:4.88E-4|Clearance:0.05||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MRS6(YOR370C)|FD-Score:3.8|P-value:7.31E-5|Clearance:0.02||SGD DESC:Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress Gene:PKC1(YBL105C)|FD-Score:5.73|P-value:4.99E-9|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE2(YPR103W)|FD-Score:-3.44|P-value:2.89E-4|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RIM2(YBR192W)|FD-Score:5.56|P-value:1.36E-8|Clearance:0.04||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPA43(YOR340C)|FD-Score:3.5|P-value:2.34E-4|Clearance:0.08||SGD DESC:RNA polymerase I subunit A43 Gene:RPP1(YHR062C)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT6(YGL048C)|FD-Score:3.2|P-value:6.89E-4|Clearance:0.05||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RRS1(YOR294W)|FD-Score:3.12|P-value:8.93E-4|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC58(YLR033W)|FD-Score:6.48|P-value:4.48E-11|Clearance:0.75||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.13|P-value:1.82E-5|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:5.11|P-value:1.60E-7|Clearance:0.59||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC10(YLR166C)|FD-Score:3.15|P-value:8.24E-4|Clearance:0.01||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEC27(YGL137W)|FD-Score:3.78|P-value:7.85E-5|Clearance:0.08||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SEN1(YLR430W)|FD-Score:3.11|P-value:9.46E-4|Clearance:0.05||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SFH1(YLR321C)|FD-Score:3.88|P-value:5.22E-5|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SLN1(YIL147C)|FD-Score:3.25|P-value:5.73E-4|Clearance:0.04||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SPC105(YGL093W)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.01||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:STS1(YIR011C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.19||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:YDL196W(YDL196W_d)|FD-Score:4.52|P-value:3.11E-6|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:5.73|P-value:5.00E-9|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YTH1(YPR107C)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3239779
Download HOP data (tab-delimited text)  (excel)
Gene:ABP140(YOR239W)|FD-Score:-4.77|P-value:9.34E-7||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ABZ1(YNR033W)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:ART5(YGR068C)|FD-Score:3.79|P-value:7.52E-5||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:ATG5(YPL149W)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BSP1(YPR171W)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CLC1(YGR167W)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG8(YML071C)|FD-Score:5.46|P-value:2.40E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COR1(YBL045C)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:CTR1(YPR124W)|FD-Score:4.88|P-value:5.33E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DBP7(YKR024C)|FD-Score:4.6|P-value:2.07E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:ENV9(YOR246C)|FD-Score:7.36|P-value:9.19E-14||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:EOS1(YNL080C)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FCJ1(YKR016W)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FUS1(YCL027W)|FD-Score:-4.92|P-value:4.25E-7||SGD DESC:Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate Gene:GCN4(YEL009C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GSY2(YLR258W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GYP1(YOR070C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HPR1(YDR138W)|FD-Score:-4.58|P-value:2.28E-6||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IRC3(YDR332W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:KRE2(YDR483W)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LRP1(YHR081W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MDM20(YOL076W)|FD-Score:5.39|P-value:3.46E-8||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MFA1(YDR461W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MTC7(YEL033W_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NEJ1(YLR265C)|FD-Score:4.35|P-value:6.88E-6||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PAC11(YDR488C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 Gene:PCI8(YIL071C)|FD-Score:4.53|P-value:3.02E-6||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCL1(YNL289W)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PFA5(YDR459C)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:RAX1(YOR301W)|FD-Score:-4.77|P-value:9.13E-7||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RIF1(YBR275C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIF2(YLR453C)|FD-Score:8.51|P-value:8.50E-18||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL20A(YMR242C)|FD-Score:6.72|P-value:9.11E-12||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SIP5(YMR140W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKN7(YHR206W)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SNF6(YHL025W)|FD-Score:-3.88|P-value:5.19E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SOL2(YCR073W-A)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SSE1(YPL106C)|FD-Score:4.45|P-value:4.30E-6||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SUS1(YBR111W-A)|FD-Score:4.82|P-value:7.14E-7||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWI4(YER111C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TDA5(YLR426W_p)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TRM44(YPL030W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:TRP1(YDR007W)|FD-Score:4.98|P-value:3.17E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VPS69(YPR087W_d)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:WTM1(YOR230W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YAL037W(YAL037W_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YDR154C(YDR154C_d)|FD-Score:5.44|P-value:2.70E-8||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIL059C(YIL059C_d)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Gene:YIM2(YMR151W_d)|FD-Score:4|P-value:3.17E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YKL102C(YKL102C_d)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YLR030W(YLR030W_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function Gene:YLR049C(YLR049C_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function Gene:YLR118C(YLR118C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YMR196W(YMR196W_p)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL195C(YNL195C_p)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR062C(YNR062C_p)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Putative membrane protein of unknown function Gene:YPR003C(YPR003C_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:ABP140(YOR239W)|FD-Score:-4.77|P-value:9.34E-7||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ABZ1(YNR033W)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:ART5(YGR068C)|FD-Score:3.79|P-value:7.52E-5||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:ATG5(YPL149W)|FD-Score:3.91|P-value:4.60E-5||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BSP1(YPR171W)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CLC1(YGR167W)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG8(YML071C)|FD-Score:5.46|P-value:2.40E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COR1(YBL045C)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain Gene:CTR1(YPR124W)|FD-Score:4.88|P-value:5.33E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DBP7(YKR024C)|FD-Score:4.6|P-value:2.07E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:ENV9(YOR246C)|FD-Score:7.36|P-value:9.19E-14||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:EOS1(YNL080C)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FCJ1(YKR016W)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FUS1(YCL027W)|FD-Score:-4.92|P-value:4.25E-7||SGD DESC:Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate Gene:GCN4(YEL009C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GSY2(YLR258W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GYP1(YOR070C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HPR1(YDR138W)|FD-Score:-4.58|P-value:2.28E-6||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IRC3(YDR332W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:KRE2(YDR483W)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LRP1(YHR081W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MDM20(YOL076W)|FD-Score:5.39|P-value:3.46E-8||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MFA1(YDR461W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MTC7(YEL033W_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NEJ1(YLR265C)|FD-Score:4.35|P-value:6.88E-6||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:PAC11(YDR488C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8 Gene:PCI8(YIL071C)|FD-Score:4.53|P-value:3.02E-6||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCL1(YNL289W)|FD-Score:-3.1|P-value:9.74E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PFA5(YDR459C)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:RAX1(YOR301W)|FD-Score:-4.77|P-value:9.13E-7||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RIF1(YBR275C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIF2(YLR453C)|FD-Score:8.51|P-value:8.50E-18||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPL20A(YMR242C)|FD-Score:6.72|P-value:9.11E-12||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SIP5(YMR140W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SKN7(YHR206W)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SNF6(YHL025W)|FD-Score:-3.88|P-value:5.19E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SOL2(YCR073W-A)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SSE1(YPL106C)|FD-Score:4.45|P-value:4.30E-6||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SUS1(YBR111W-A)|FD-Score:4.82|P-value:7.14E-7||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWI4(YER111C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TDA5(YLR426W_p)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TRM44(YPL030W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:TRP1(YDR007W)|FD-Score:4.98|P-value:3.17E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VPS69(YPR087W_d)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:WTM1(YOR230W)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YAL037W(YAL037W_p)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YDR154C(YDR154C_d)|FD-Score:5.44|P-value:2.70E-8||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIL059C(YIL059C_d)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Gene:YIM2(YMR151W_d)|FD-Score:4|P-value:3.17E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YKL102C(YKL102C_d)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site Gene:YLR030W(YLR030W_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function Gene:YLR049C(YLR049C_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function Gene:YLR118C(YLR118C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YMR196W(YMR196W_p)|FD-Score:5.94|P-value:1.40E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL195C(YNL195C_p)|FD-Score:3.19|P-value:6.99E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR062C(YNR062C_p)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Putative membrane protein of unknown function Gene:YPR003C(YPR003C_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W6.484.48E-110.75RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YBL105C5.734.99E-96.33E-4PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YGR114C_d5.735.00E-90.17YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YBR192W5.561.36E-80.04RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YJL014W5.511.75E-80.40CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YFR037C5.111.60E-70.59RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YDL196W_d4.523.11E-60.25YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YDR141C4.279.63E-60.14DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
YCR052W4.131.82E-50.16RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YMR168C3.973.62E-50.09CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YLR321C3.885.22E-50.08SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YOR370C3.807.31E-50.02MRS6Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
YGL137W3.787.85E-50.08SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YIR011C3.701.06E-40.19STS1Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
YPR107C3.522.19E-40.02YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR453C8.518.50E-18RIF2Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YOR246C7.369.19E-14ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YMR242C6.729.11E-12RPL20ARibosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
YMR196W_p5.941.40E-9YMR196W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
YML071C5.462.40E-8COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR154C_d5.442.70E-8YDR154C_dDubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein
YOL076W5.393.46E-8MDM20Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly
YNR033W5.201.01E-7ABZ1Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
YDR007W4.983.17E-7TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDL020C4.963.46E-7RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YPR124W4.885.33E-7CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YBR111W-A4.827.14E-7SUS1Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP
YKR024C4.602.07E-6DBP7Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
YIL071C4.533.02E-6PCI8Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
YPL106C4.454.30E-6SSE1ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1171
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1778.81E-43SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.121212RSC & ERG11
0.1612.47E-35SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0428571RSC complex & mRNA processing
0.1582.38E-34SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.106061RSC complex & mRNA processing
0.1577.08E-34SGTC_7093807-1509 243.0 μMChemDiv (Drug-like library)18866770.028169RSC complex & mRNA processing
0.1512.14E-31SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0980392
0.1453.34E-29SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0285714heme biosynthesis & mitochondrial translocase
0.1427.06E-28SGTC_2766oltipraz 83.9 μMMiscellaneous473180.127273heme requiring
0.1411.42E-27SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.101695RSC complex & mRNA processing
0.1352.91E-25SGTC_2510benzyl isothiocyanate 8.3 μMMicrosource (Natural product library)23460.08
0.1322.14E-24SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.119403RSC complex & mRNA processing
0.1315.17E-24SGTC_2881315-0019 35.1 μMChemDiv (Drug-like library)68114740.0746269RSC & ERG11
0.1292.17E-23SGTC_331k048-0133 24.5 μMChemDiv (Drug-like library)68135820.206897RSC & ERG11
0.1278.42E-23SGTC_23469031566 143.8 μMChembridge (Fragment library)47710860.0740741Golgi
0.1262.33E-22SGTC_268camptothecin 39.6 μMMiscellaneous243600.0853659DNA damage response
0.1262.85E-22SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0655738Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8480368-008985.6 μM0.545455762535ChemDiv (Drug-like library)267.110722.79212iron homeostasis
SGTC_2929901334824.6 μM0.42995952Chembridge (Drug-like library)310.175223.99912
SGTC_21535569715200 μM0.3958332054701Chembridge (Fragment library)281.13732.79912iron homeostasis
SGTC_3118912425049.47 μM0.3773581504786Chembridge (Drug-like library)317.16944.12912
SGTC_2967908953849.85 μM0.37037728216Chembridge (Drug-like library)300.663633.0715
SGTC_1758st04294275.24 μM0.346939669317TimTec (Natural product derivative library)262.089362.07413
SGTC_2933902679432.47 μM0.33898317586027Chembridge (Drug-like library)359.421044.00505
SGTC_12961068-010211.9 μM0.320755767053ChemDiv (Drug-like library)310.175224.02712
SGTC_2979901255245.46 μM0.32075517100905Chembridge (Drug-like library)282.724343.03612
SGTC_7211187-1332279 μM0.30612225730ChemDiv (Drug-like library)281.13733.96522cell wall signaling