1021-1431

3-(4-bromophenyl)sulfanyl-1-phenylpyrrolidine-2,5-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1179
Screen concentration 9.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 2840958
SMILES C1C(C(=O)N(C1=O)C2=CC=CC=C2)SC3=CC=C(C=C3)Br
Standardized SMILES Brc1ccc(SC2CC(=O)N(C2=O)c3ccccc3)cc1
Molecular weight 362.241
ALogP 3.82
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.76
% growth inhibition (Hom. pool) 4.46


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2840958
Download HIP data (tab-delimited text)  (excel)
Gene:CCT7(YJL111W)|FD-Score:4.68|P-value:1.44E-6|Clearance:0.39||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC19(YAL038W)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.03||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CWC24(YLR323C)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.04||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DED81(YHR019C)|FD-Score:4.69|P-value:1.40E-6|Clearance:0.01||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:DRE2(YKR071C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.04||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:ESP1(YGR098C)|FD-Score:-3.89|P-value:5.10E-5|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD2(YGR083C)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.04||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:KOG1(YHR186C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.58||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MET30(YIL046W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.11||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NSL1(YPL233W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.1||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUP57(YGR119C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:RET1(YOR207C)|FD-Score:8.08|P-value:3.29E-16|Clearance:1.78||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC19(YNL113W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.05||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPP1(YHR062C)|FD-Score:6.47|P-value:4.89E-11|Clearance:1.78||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RVB2(YPL235W)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.12||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:TCP1(YDR212W)|FD-Score:4.28|P-value:9.17E-6|Clearance:0.1||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:CCT7(YJL111W)|FD-Score:4.68|P-value:1.44E-6|Clearance:0.39||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC19(YAL038W)|FD-Score:3.25|P-value:5.78E-4|Clearance:0.03||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CWC24(YLR323C)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.04||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DED81(YHR019C)|FD-Score:4.69|P-value:1.40E-6|Clearance:0.01||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:DRE2(YKR071C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.04||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:ESP1(YGR098C)|FD-Score:-3.89|P-value:5.10E-5|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GCD2(YGR083C)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.04||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:KOG1(YHR186C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.58||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MET30(YIL046W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.11||SGD DESC:F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus Gene:NSL1(YPL233W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.1||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUP57(YGR119C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:RET1(YOR207C)|FD-Score:8.08|P-value:3.29E-16|Clearance:1.78||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC19(YNL113W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.05||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPP1(YHR062C)|FD-Score:6.47|P-value:4.89E-11|Clearance:1.78||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RVB2(YPL235W)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.12||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:TCP1(YDR212W)|FD-Score:4.28|P-value:9.17E-6|Clearance:0.1||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2840958
Download HOP data (tab-delimited text)  (excel)
Gene:APM4(YOL062C)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARO7(YPR060C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG27(YJL178C)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:ATG3(YNR007C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CIN1(YOR349W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CLA4(YNL298W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG7(YGL005C)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSH1(YBR161W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DID2(YKR035W-A)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:EDE1(YBL047C)|FD-Score:8.18|P-value:1.44E-16||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ERV14(YGL054C)|FD-Score:7.75|P-value:4.73E-15||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAR1(YJL157C)|FD-Score:3.77|P-value:8.11E-5||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FEN1(YCR034W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIS1(YIL065C)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FMS1(YMR020W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:GET1(YGL020C)|FD-Score:7.85|P-value:2.02E-15||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:HHT2(YNL031C)|FD-Score:-3.77|P-value:8.23E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HMG2(YLR450W)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:HOP1(YIL072W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:LEE1(YPL054W)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LEU1(YGL009C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:LGE1(YPL055C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LTE1(YAL024C)|FD-Score:6.17|P-value:3.36E-10||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAF1(YDR005C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MNL1(YHR204W)|FD-Score:4.88|P-value:5.28E-7||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MON2(YNL297C)|FD-Score:-4.61|P-value:2.00E-6||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRH4(YGL064C)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRPL49(YJL096W)|FD-Score:8.85|P-value:4.38E-19||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSR1(YHR091C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MYO4(YAL029C)|FD-Score:4.79|P-value:8.27E-7||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:OAR1(YKL055C)|FD-Score:4.3|P-value:8.49E-6||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PAC2(YER007W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PDR8(YLR266C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PET309(YLR067C)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PHO81(YGR233C)|FD-Score:4.46|P-value:4.11E-6||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PIN4(YBL051C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POL32(YJR043C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRD1(YCL057W)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PRO1(YDR300C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS1(YKL181W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RGL1(YPL066W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIF2(YLR453C)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:ROG3(YFR022W)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPB9(YGL070C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL14A(YKL006W)|FD-Score:7.16|P-value:4.07E-13||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL19B(YBL027W)|FD-Score:6.92|P-value:2.28E-12||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL4A(YBR031W)|FD-Score:9.66|P-value:2.32E-22||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.34|P-value:7.22E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS10B(YMR230W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:3.72|P-value:9.81E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SBP1(YHL034C)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SCO1(YBR037C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SCS7(YMR272C)|FD-Score:-4|P-value:3.18E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SDH4(YDR178W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SEA4(YBL104C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SFP1(YLR403W)|FD-Score:5.02|P-value:2.52E-7||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SIN4(YNL236W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SLT2(YHR030C)|FD-Score:-3.9|P-value:4.85E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNA2(YDR525W-A)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOD1(YJR104C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPG1(YGR236C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SUT1(YGL162W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWC5(YBR231C)|FD-Score:6.72|P-value:9.31E-12||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWF1(YDR126W)|FD-Score:6.3|P-value:1.52E-10||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TEC1(YBR083W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member Gene:THI21(YPL258C)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TIP41(YPR040W)|FD-Score:5.36|P-value:4.18E-8||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TMA23(YMR269W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TOP1(YOL006C)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TPK1(YJL164C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:7.75|P-value:4.43E-15||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:6.96|P-value:1.66E-12||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP9(YER098W)|FD-Score:4.27|P-value:9.70E-6||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VAN1(YML115C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VBA2(YBR293W)|FD-Score:4.98|P-value:3.19E-7||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS21(YOR089C)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS71(YML041C)|FD-Score:4.78|P-value:8.81E-7||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL012C(YBL012C_d)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR277C(YBR277C_d)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YDR010C(YDR010C_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL034C(YGL034C_d)|FD-Score:6.29|P-value:1.57E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHI9(YHR029C)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YIL028W(YIL028W_d)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL069W(YKL069W)|FD-Score:6.05|P-value:7.13E-10||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL097C(YKL097C_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKL169C(YKL169C_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR363W-A(YLR363W-A_p)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YMR144W(YMR144W_p)|FD-Score:4.83|P-value:6.78E-7||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YMR147W(YMR147W_p)|FD-Score:9.5|P-value:1.04E-21||SGD DESC:Putative protein of unknown function Gene:YMR155W(YMR155W_p)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNR048W(YNR048W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOL057W(YOL057W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL106W(YOL106W_d)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL216W(YPL216W_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPR071W(YPR071W_p)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C Gene:APM4(YOL062C)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARO7(YPR060C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG27(YJL178C)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:ATG3(YNR007C)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CIN1(YOR349W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CLA4(YNL298W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG7(YGL005C)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSH1(YBR161W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DID2(YKR035W-A)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:EDE1(YBL047C)|FD-Score:8.18|P-value:1.44E-16||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ERV14(YGL054C)|FD-Score:7.75|P-value:4.73E-15||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAR1(YJL157C)|FD-Score:3.77|P-value:8.11E-5||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FEN1(YCR034W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIS1(YIL065C)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FMS1(YMR020W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis Gene:GET1(YGL020C)|FD-Score:7.85|P-value:2.02E-15||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:HHT2(YNL031C)|FD-Score:-3.77|P-value:8.23E-5||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HMG2(YLR450W)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:HOP1(YIL072W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:LEE1(YPL054W)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LEU1(YGL009C)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:LGE1(YPL055C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LTE1(YAL024C)|FD-Score:6.17|P-value:3.36E-10||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAF1(YDR005C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MNL1(YHR204W)|FD-Score:4.88|P-value:5.28E-7||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MON2(YNL297C)|FD-Score:-4.61|P-value:2.00E-6||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRH4(YGL064C)|FD-Score:4.35|P-value:6.69E-6||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRPL49(YJL096W)|FD-Score:8.85|P-value:4.38E-19||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSR1(YHR091C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MYO4(YAL029C)|FD-Score:4.79|P-value:8.27E-7||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:OAR1(YKL055C)|FD-Score:4.3|P-value:8.49E-6||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PAC2(YER007W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PDR8(YLR266C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PET309(YLR067C)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PHO81(YGR233C)|FD-Score:4.46|P-value:4.11E-6||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PIN4(YBL051C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POL32(YJR043C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRD1(YCL057W)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PRO1(YDR300C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PRO2(YOR323C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS1(YKL181W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RGL1(YPL066W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIF2(YLR453C)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:ROG3(YFR022W)|FD-Score:3.11|P-value:9.47E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPB9(YGL070C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL14A(YKL006W)|FD-Score:7.16|P-value:4.07E-13||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL19B(YBL027W)|FD-Score:6.92|P-value:2.28E-12||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPL4A(YBR031W)|FD-Score:9.66|P-value:2.32E-22||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.34|P-value:7.22E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS10B(YMR230W)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:3.72|P-value:9.81E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RPS6A(YPL090C)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SBP1(YHL034C)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SCO1(YBR037C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SCS7(YMR272C)|FD-Score:-4|P-value:3.18E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SDH4(YDR178W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SEA4(YBL104C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SFP1(YLR403W)|FD-Score:5.02|P-value:2.52E-7||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SIN4(YNL236W)|FD-Score:4.07|P-value:2.34E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SLT2(YHR030C)|FD-Score:-3.9|P-value:4.85E-5||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNA2(YDR525W-A)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOD1(YJR104C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPG1(YGR236C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SUT1(YGL162W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Transcription factor of the Zn2Cys6 family involved in sterol uptake; involved in induction of hypoxic gene expression; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SWC5(YBR231C)|FD-Score:6.72|P-value:9.31E-12||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWF1(YDR126W)|FD-Score:6.3|P-value:1.52E-10||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TEC1(YBR083W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member Gene:THI21(YPL258C)|FD-Score:-4.11|P-value:1.95E-5||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TIP41(YPR040W)|FD-Score:5.36|P-value:4.18E-8||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TMA23(YMR269W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TOP1(YOL006C)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:TPK1(YJL164C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:7.75|P-value:4.43E-15||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:6.96|P-value:1.66E-12||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP9(YER098W)|FD-Score:4.27|P-value:9.70E-6||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VAN1(YML115C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VBA2(YBR293W)|FD-Score:4.98|P-value:3.19E-7||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS21(YOR089C)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS71(YML041C)|FD-Score:4.78|P-value:8.81E-7||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL012C(YBL012C_d)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR277C(YBR277C_d)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YDR010C(YDR010C_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL034C(YGL034C_d)|FD-Score:6.29|P-value:1.57E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHI9(YHR029C)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YIL028W(YIL028W_d)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL069W(YKL069W)|FD-Score:6.05|P-value:7.13E-10||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKL097C(YKL097C_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKL169C(YKL169C_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR363W-A(YLR363W-A_p)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YMR144W(YMR144W_p)|FD-Score:4.83|P-value:6.78E-7||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YMR147W(YMR147W_p)|FD-Score:9.5|P-value:1.04E-21||SGD DESC:Putative protein of unknown function Gene:YMR155W(YMR155W_p)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YNR048W(YNR048W)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOL057W(YOL057W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL106W(YOL106W_d)|FD-Score:4.56|P-value:2.54E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL216W(YPL216W_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPR071W(YPR071W_p)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Putative membrane protein; YPR071W is not an essential gene; YPR071W has paralog YIL029C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR207C8.083.29E-161.78RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YHR062C6.474.89E-111.78RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR019C4.691.40E-60.01DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YJL111W4.681.44E-60.40CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YDR212W4.289.17E-60.10TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YHR186C4.191.41E-50.58KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YNL113W3.611.54E-40.05RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YPL233W3.561.86E-40.10NSL1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YKR071C3.462.72E-40.04DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YLR323C3.423.18E-40.04CWC24Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
YPL235W3.373.72E-40.12RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YAL038W3.255.78E-40.03CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YGR119C3.216.53E-40.01NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YIL046W3.206.81E-40.11MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
YGR083C3.099.95E-40.04GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR031W9.662.32E-22RPL4ARibosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication
YMR147W_p9.501.04E-21YMR147W_pPutative protein of unknown function
YJL096W8.854.38E-19MRPL49Mitochondrial ribosomal protein of the large subunit
YBL047C8.181.44E-16EDE1Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death
YGL020C7.852.02E-15GET1Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
YBR058C-A7.754.43E-15TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGL054C7.754.73E-15ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YKL006W7.164.07E-13RPL14ARibosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
YML124C6.961.66E-12TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YBL027W6.922.28E-12RPL19BRibosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication
YBR231C6.729.31E-12SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YDR126W6.301.52E-10SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YGL034C_d6.291.57E-10YGL034C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YAL024C6.173.36E-10LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YKL069W6.057.13E-10YKL069WMethionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress

GO enrichment analysis for SGTC_1179
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2901.80E-113SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.4
0.2811.81E-107SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.108108
0.2637.58E-94SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.75
0.2449.58E-81SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.109589
0.2351.70E-74SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0983607
0.2344.18E-74SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.0923077
0.2178.38E-64SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.123288
0.2094.62E-59SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.0724638
0.2022.50E-55SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0645161TSC3-RPN4
0.1923.04E-50SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0634921TSC3-RPN4
0.1817.03E-45SGTC_29529073731 13.8 μMChembridge (Drug-like library)164573530.114286tubulin folding & SWR complex
0.1756.24E-42SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.289855TSC3-RPN4
0.1756.33E-42SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0967742TSC3-RPN4
0.1694.77E-39SGTC_21515563267 85.4 μMChembridge (Fragment library)53108240.0833333
0.1697.19E-39SGTC_21125488546 200.0 μMChembridge (Fragment library)6007570.0517241TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2474532157043.32 μM0.752841384Miscellaneous297.37153.55303
SGTC_5891227-007034.1 μM0.752841384ChemDiv (Drug-like library)297.37153.55303
SGTC_2461575530226.15 μM0.5918372788904Miscellaneous406.250483.44615
SGTC_14013807-46797.6 μM0.5416673291456ChemDiv (Drug-like library)274.29841.36915tubulin folding & SWR complex
SGTC_2479561735343.85 μM0.5416672731746Miscellaneous327.354422.69715
SGTC_2458580890716.39 μM0.4444442731747Miscellaneous361.799483.36215
SGTC_2469565501990.81 μM0.4444442788239Miscellaneous341.3813.18315
SGTC_2465580924233.21 μM0.4210532731748Miscellaneous357.38042.68116
SGTC_247358545938.94 μM0.4210532872951Miscellaneous410.486083.77716
SGTC_2456577035974.46 μM0.406783118787Miscellaneous412.45892.54717