1222-0449

3-(4-pentylphenyl)-1H-pyridazin-6-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1181
Screen concentration 220.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 658318
SMILES CCCCCC1=CC=C(C=C1)C2=NNC(=O)C=C2
Standardized SMILES CCCCCc1ccc(cc1)c2ccc(O)nn2
Molecular weight 242.3162
ALogP 3.76
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.23
% growth inhibition (Hom. pool) 8.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 658318
Download HIP data (tab-delimited text)  (excel)
Gene:CCT6(YDR188W)|FD-Score:-3.87|P-value:5.52E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC37(YDR168W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.23||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:DYS1(YHR068W)|FD-Score:3.47|P-value:2.65E-4|Clearance:0.06||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:LSM4(YER112W)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NSA2(YER126C)|FD-Score:-3.79|P-value:7.44E-5|Clearance:0||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:NSE3(YDR288W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.23||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:POP4(YBR257W)|FD-Score:3.4|P-value:3.35E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RAD53(YPL153C)|FD-Score:3.16|P-value:8.01E-4|Clearance:0.2||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RLI1(YDR091C)|FD-Score:3.21|P-value:6.54E-4|Clearance:0.03||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPP1(YHR062C)|FD-Score:3.96|P-value:3.77E-5|Clearance:0.23||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRS1(YOR294W)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.02||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC58(YLR033W)|FD-Score:-3.31|P-value:4.71E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSE1(YML049C)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.12||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:YGR114C(YGR114C_d)|FD-Score:3.81|P-value:7.01E-5|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:CCT6(YDR188W)|FD-Score:-3.87|P-value:5.52E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC37(YDR168W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.23||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:DYS1(YHR068W)|FD-Score:3.47|P-value:2.65E-4|Clearance:0.06||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:LSM4(YER112W)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:NSA2(YER126C)|FD-Score:-3.79|P-value:7.44E-5|Clearance:0||SGD DESC:Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA Gene:NSE3(YDR288W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.23||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:POP4(YBR257W)|FD-Score:3.4|P-value:3.35E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RAD53(YPL153C)|FD-Score:3.16|P-value:8.01E-4|Clearance:0.2||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RLI1(YDR091C)|FD-Score:3.21|P-value:6.54E-4|Clearance:0.03||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPP1(YHR062C)|FD-Score:3.96|P-value:3.77E-5|Clearance:0.23||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRS1(YOR294W)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.02||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC58(YLR033W)|FD-Score:-3.31|P-value:4.71E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC9(YML127W)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSE1(YML049C)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.12||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:YGR114C(YGR114C_d)|FD-Score:3.81|P-value:7.01E-5|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 658318
Download HOP data (tab-delimited text)  (excel)
Gene:AIM25(YJR100C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AKL1(YBR059C)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:ALG5(YPL227C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARO1(YDR127W)|FD-Score:6.12|P-value:4.55E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BCK2(YER167W)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CLU1(YMR012W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:DAL81(YIR023W)|FD-Score:14.9|P-value:1.36E-50||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBP1(YPL119C)|FD-Score:5.09|P-value:1.81E-7||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DOM34(YNL001W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DOT6(YER088C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DPH5(YLR172C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:ERT1(YBR239C_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:FKS3(YMR306W)|FD-Score:-4.53|P-value:2.99E-6||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IOC4(YMR044W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRC20(YLR247C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISR1(YPR106W)|FD-Score:5.33|P-value:4.79E-8||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:LSB1(YGR136W)|FD-Score:5.58|P-value:1.17E-8||SGD DESC:Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress Gene:LSC1(YOR142W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MGR1(YCL044C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MID2(YLR332W)|FD-Score:5.2|P-value:9.71E-8||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:NPP2(YEL016C)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:PBY1(YBR094W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress Gene:PEP12(YOR036W)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:RAD14(YMR201C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD23(YEL037C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RAD27(YKL113C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RHO4(YKR055W)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RNH1(YMR234W)|FD-Score:5.49|P-value:2.07E-8||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL13B(YMR142C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL4A(YBR031W)|FD-Score:4.45|P-value:4.27E-6||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:SAM1(YLR180W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SHE1(YBL031W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SKN7(YHR206W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SMY2(YBR172C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:-7.29|P-value:1.52E-13||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNF6(YHL025W)|FD-Score:-3.86|P-value:5.77E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SYP1(YCR030C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TDA11(YHR159W_p)|FD-Score:-3.89|P-value:5.08E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP1(YDR007W)|FD-Score:6.25|P-value:2.04E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.88|P-value:2.08E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:12.6|P-value:8.42E-37||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.56|P-value:2.08E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS33(YOR115C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TSR2(YLR435W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:VIP1(YLR410W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:YAP1801(YHR161C)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR146C-A(YGR146C-A_p)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Putative protein of unknown function Gene:YGR169C-A(YGR169C-A_p)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHR003C(YHR003C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YHR049C-A(YHR049C-A_d)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL161W(YIL161W_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YIR044C(YIR044C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL181W(YJL181W_p)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR312C(YLR312C_p)|FD-Score:-5.1|P-value:1.72E-7||SGD DESC:Putative protein of unknown function Gene:YML007C-A(YML007C-A_p)|FD-Score:5.01|P-value:2.71E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YOR114W(YOR114W_p)|FD-Score:-4.13|P-value:1.78E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPR077C(YPR077C_d)|FD-Score:4.39|P-value:5.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR084W(YPR084W_p)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Putative protein of unknown function Gene:YPR099C(YPR099C_d)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPT7(YML001W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:YSW1(YBR148W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:YVH1(YIR026C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ZAP1(YJL056C)|FD-Score:5.98|P-value:1.14E-9||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:AIM25(YJR100C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AKL1(YBR059C)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization Gene:ALG5(YPL227C)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARO1(YDR127W)|FD-Score:6.12|P-value:4.55E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BCK2(YER167W)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CLU1(YMR012W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:DAL81(YIR023W)|FD-Score:14.9|P-value:1.36E-50||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBP1(YPL119C)|FD-Score:5.09|P-value:1.81E-7||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DOM34(YNL001W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DOT6(YER088C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:DPH5(YLR172C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:ERT1(YBR239C_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:FKS3(YMR306W)|FD-Score:-4.53|P-value:2.99E-6||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IOC4(YMR044W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRC20(YLR247C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISR1(YPR106W)|FD-Score:5.33|P-value:4.79E-8||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:LSB1(YGR136W)|FD-Score:5.58|P-value:1.17E-8||SGD DESC:Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress Gene:LSC1(YOR142W)|FD-Score:3.73|P-value:9.71E-5||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:MGR1(YCL044C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MID2(YLR332W)|FD-Score:5.2|P-value:9.71E-8||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:NPP2(YEL016C)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:PBY1(YBR094W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Putative tubulin tyrosine ligase associated with P-bodies; forms cytoplasmic foci upon DNA replication stress Gene:PEP12(YOR036W)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:RAD14(YMR201C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD23(YEL037C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RAD27(YKL113C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RHO4(YKR055W)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RNH1(YMR234W)|FD-Score:5.49|P-value:2.07E-8||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RPL13B(YMR142C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL4A(YBR031W)|FD-Score:4.45|P-value:4.27E-6||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:SAM1(YLR180W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication Gene:SHE1(YBL031W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SKN7(YHR206W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SMY2(YBR172C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Protein of unknown function involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication Gene:SNF5(YBR289W)|FD-Score:-7.29|P-value:1.52E-13||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNF6(YHL025W)|FD-Score:-3.86|P-value:5.77E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SYP1(YCR030C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TDA11(YHR159W_p)|FD-Score:-3.89|P-value:5.08E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP1(YDR007W)|FD-Score:6.25|P-value:2.04E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.88|P-value:2.08E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:12.6|P-value:8.42E-37||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.56|P-value:2.08E-14||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRS33(YOR115C)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TSR2(YLR435W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:VIP1(YLR410W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:YAP1801(YHR161C)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR146C-A(YGR146C-A_p)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Putative protein of unknown function Gene:YGR169C-A(YGR169C-A_p)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHR003C(YHR003C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YHR049C-A(YHR049C-A_d)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL161W(YIL161W_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YIR044C(YIR044C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YJL181W(YJL181W_p)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR312C(YLR312C_p)|FD-Score:-5.1|P-value:1.72E-7||SGD DESC:Putative protein of unknown function Gene:YML007C-A(YML007C-A_p)|FD-Score:5.01|P-value:2.71E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YOR114W(YOR114W_p)|FD-Score:-4.13|P-value:1.78E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:YPR077C(YPR077C_d)|FD-Score:4.39|P-value:5.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR084W(YPR084W_p)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Putative protein of unknown function Gene:YPR099C(YPR099C_d)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPT7(YML001W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:YSW1(YBR148W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:YVH1(YIR026C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ZAP1(YJL056C)|FD-Score:5.98|P-value:1.14E-9||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C3.963.77E-50.23RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR114C_d3.817.01E-50.23YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YDR168W3.701.07E-40.23CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
YDR288W3.701.09E-40.23NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YHR068W3.472.65E-40.06DYS1Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
YBR257W3.403.35E-40.05POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YOR294W3.354.00E-40.02RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YML049C3.334.32E-40.12RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
YDR091C3.216.54E-40.03RLI1Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species
YER112W3.197.16E-40.03LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YPL153C3.168.01E-40.20RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YBR124W_d2.950.001570.13YBR124W_dPutative protein of unknown function
YHR023W2.830.002350.02MYO1Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
YJL034W2.800.002540.01KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YLR103C2.790.002650.04CDC45DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W14.901.36E-50DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YKL211C12.608.42E-37TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W7.562.08E-14TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR007W6.252.04E-10TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR127W6.124.55E-10ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YJL056C5.981.14E-9ZAP1Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
YER090W5.882.08E-9TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YGR136W5.581.17E-8LSB1Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
YMR234W5.492.07E-8RNH1Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201)
YPR106W5.334.79E-8ISR1Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
YLR332W5.209.71E-8MID2O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
YPL119C5.091.81E-7DBP1Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress
YML007C-A_p5.012.71E-7YML007C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
YPR099C_d4.681.46E-6YPR099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W
YER088C4.592.24E-6DOT6Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress

GO enrichment analysis for SGTC_1181
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3707.42E-191SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.078125
0.3651.01E-185SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0481928
0.3653.20E-185SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0634921
0.3605.18E-180SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0350877
0.3594.59E-179SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.146667
0.3512.02E-170SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.129032
0.3479.80E-167SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.0793651
0.3401.98E-159SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.245283
0.3326.62E-152SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.0769231
0.3329.31E-152SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.045977
0.3244.40E-144SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.0714286
0.3192.62E-139SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.166667
0.3188.77E-139SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.0909091amide catabolism
0.3171.55E-137SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.057971
0.3142.22E-135SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.0882353

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_11821222-0449220 μM1658318ChemDiv (Drug-like library)242.316223.75812
SGTC_810411-00469.97 μM0.36956512925265ChemDiv (Drug-like library)297.230645.4701
SGTC_790052-000794.09 μM0.3469392801472ChemDiv (Drug-like library)276.370664.63813
SGTC_9591222-0044107 μM0.3469396083612ChemDiv (Drug-like library)232.31814.68512
SGTC_9581222-000733 μM0.347312461ChemDiv (Drug-like library)246.344685.14112
SGTC_6641222-005414.9 μM0.3272736813991ChemDiv (Drug-like library)287.3717833.72413sphingolipid biosynthesis & PDR1
SGTC_820054-0107293.76 μM0.3207553104697ChemDiv (Drug-like library)340.412866.11514
SGTC_1121fusaric acid630.51 nM0.3061223442TimTec (Natural product library)179.215722.59213
SGTC_2961222-003974.9 μM0.35984176ChemDiv (Drug-like library)218.291524.22812
SGTC_2969909140828.7 μM0.2857144286311Chembridge (Drug-like library)254.326923.31812