1222-0449

3-(4-pentylphenyl)-1H-pyridazin-6-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1182
Screen concentration 220.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 658318
SMILES CCCCCC1=CC=C(C=C1)C2=NNC(=O)C=C2
Standardized SMILES CCCCCc1ccc(cc1)c2ccc(O)nn2
Molecular weight 242.3162
ALogP 3.76
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.23
% growth inhibition (Hom. pool) 8.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 658318
Download HIP data (tab-delimited text)  (excel)
Gene:ILS1(YBL076C)|FD-Score:-3.99|P-value:3.27E-5|Clearance:0||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:PAB1(YER165W)|FD-Score:-3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PRE5(YMR314W)|FD-Score:-5.6|P-value:1.07E-8|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RRN3(YKL125W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.08||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SMD2(YLR275W)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.5||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SOG2(YOR353C)|FD-Score:4.74|P-value:1.09E-6|Clearance:0.5||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:SUB2(YDL084W)|FD-Score:4.29|P-value:9.11E-6|Clearance:0.5||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:3.74|P-value:9.11E-5|Clearance:0.4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDR413C(YDR413C_d)|FD-Score:3.11|P-value:9.50E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YJL195C(YJL195C_d)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:ILS1(YBL076C)|FD-Score:-3.99|P-value:3.27E-5|Clearance:0||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:PAB1(YER165W)|FD-Score:-3.15|P-value:8.04E-4|Clearance:0||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PRE5(YMR314W)|FD-Score:-5.6|P-value:1.07E-8|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:RRN3(YKL125W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.08||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:SMD2(YLR275W)|FD-Score:4.24|P-value:1.12E-5|Clearance:0.5||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SOG2(YOR353C)|FD-Score:4.74|P-value:1.09E-6|Clearance:0.5||SGD DESC:Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis Gene:SUB2(YDL084W)|FD-Score:4.29|P-value:9.11E-6|Clearance:0.5||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:3.74|P-value:9.11E-5|Clearance:0.4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDR413C(YDR413C_d)|FD-Score:3.11|P-value:9.50E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YJL195C(YJL195C_d)|FD-Score:3.26|P-value:5.51E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 658318
Download HOP data (tab-delimited text)  (excel)
Gene:AIM24(YJR080C)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:ARC1(YGL105W)|FD-Score:5.73|P-value:4.91E-9||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARG1(YOL058W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO9(YHR137W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATP10(YLR393W)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCS1(YDR375C)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:COG8(YML071C)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ10(YOL008W)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COQ2(YNR041C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:DPH6(YLR143W_p)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene Gene:ETR1(YBR026C)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FAR8(YMR029C)|FD-Score:3.72|P-value:9.94E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FET4(YMR319C)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FLO8(YER109C)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:GCN4(YEL009C)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:IRC13(YOR235W_d)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:MBP1(YDL056W)|FD-Score:4.34|P-value:7.23E-6||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:NAM2(YLR382C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NRM1(YNR009W)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:PDA1(YER178W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PDR15(YDR406W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PRO2(YOR323C)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSD2(YGR170W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:PUF3(YLL013C)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RCK2(YLR248W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RNQ1(YCL028W)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPL24B(YGR148C)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:6.95|P-value:1.84E-12||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:6.13|P-value:4.35E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:4.68|P-value:1.40E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RVS161(YCR009C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SLA1(YBL007C)|FD-Score:6.43|P-value:6.23E-11||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SNL1(YIL016W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SPS18(YNL204C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRN2(YLR119W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSO1(YPL232W)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STD1(YOR047C)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TPO3(YPR156C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRS85(YDR108W)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:YBR184W(YBR184W_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBT1(YLL048C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR015C(YDR015C_d)|FD-Score:-3.92|P-value:4.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YFL054C(YFL054C_p)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YHR035W(YHR035W_p)|FD-Score:4.88|P-value:5.34E-7||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YLR122C(YLR122C_d)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YNL046W(YNL046W_p)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function Gene:YNR014W(YNR014W_p)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YOL159C-A(YOL159C-A)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR283W(YOR283W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YOR309C(YOR309C_d)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPL182C(YPL182C_d)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YRO2(YBR054W)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:AIM24(YJR080C)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:ARC1(YGL105W)|FD-Score:5.73|P-value:4.91E-9||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARG1(YOL058W)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate Gene:ARO9(YHR137W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATP10(YLR393W)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCS1(YDR375C)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases Gene:COG8(YML071C)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ10(YOL008W)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COQ2(YNR041C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:DPH6(YLR143W_p)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene Gene:ETR1(YBR026C)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FAR8(YMR029C)|FD-Score:3.72|P-value:9.94E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FET4(YMR319C)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FLO8(YER109C)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:GCN4(YEL009C)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:IRC13(YOR235W_d)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:MBP1(YDL056W)|FD-Score:4.34|P-value:7.23E-6||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:NAM2(YLR382C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NRM1(YNR009W)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:PDA1(YER178W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose Gene:PDR15(YDR406W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element Gene:PRO2(YOR323C)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSD2(YGR170W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine Gene:PUF3(YLL013C)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RCK2(YLR248W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RNQ1(YCL028W)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPL24B(YGR148C)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:6.95|P-value:1.84E-12||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:6.13|P-value:4.35E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:4.68|P-value:1.40E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RVS161(YCR009C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SLA1(YBL007C)|FD-Score:6.43|P-value:6.23E-11||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SNL1(YIL016W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SPS18(YNL204C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SRN2(YLR119W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSO1(YPL232W)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STD1(YOR047C)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:TPO3(YPR156C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRS85(YDR108W)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:YBR184W(YBR184W_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YBT1(YLL048C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR015C(YDR015C_d)|FD-Score:-3.92|P-value:4.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YFL054C(YFL054C_p)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YHR035W(YHR035W_p)|FD-Score:4.88|P-value:5.34E-7||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YLR122C(YLR122C_d)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YNL046W(YNL046W_p)|FD-Score:4.35|P-value:6.78E-6||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Putative protein of unknown function Gene:YNR014W(YNR014W_p)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YOL159C-A(YOL159C-A)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR283W(YOR283W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YOR309C(YOR309C_d)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPL182C(YPL182C_d)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YRO2(YBR054W)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Protein of unknown function with similarity to archaeal rhodopsins; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; transcriptionally regulated by Haa1p; YRO2 has a paralog, MRH1, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR353C4.741.09E-60.50SOG2Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
YDL084W4.299.11E-60.50SUB2Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
YLR275W4.241.12E-50.50SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YML085C3.749.11E-50.40TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YKL125W3.344.17E-40.08RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YJL195C_d3.265.51E-40.16YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YDR413C_d3.119.50E-40.08YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YJL086C_d3.030.001240.09YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YBR049C2.930.001680.16REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YCL031C2.770.002770.11RRP7Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
YMR309C2.660.003890.01NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YDR407C2.650.004060.06TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YOR157C2.590.004800.05PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YGR145W2.540.005500.08ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YJL026W2.460.006890.03RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML073C6.951.84E-12RPL6ARibosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
YBL007C6.436.23E-11SLA1Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
YML026C6.134.35E-10RPS18BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YGL105W5.734.91E-9ARC1Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress
YHR035W_p4.885.34E-7YHR035W_pPutative protein of unknown function; not an essential gene
YLR382C4.721.19E-6NAM2Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance
YLR264W4.681.40E-6RPS28BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
YLR122C_d4.661.60E-6YLR122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C
YLL013C4.562.55E-6PUF3Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
YBR046C4.552.70E-6ZTA1NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin
YDR375C4.533.00E-6BCS1Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
YOL008W4.415.24E-6COQ10Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
YBR026C4.385.88E-6ETR12-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis
YNL046W_p4.356.78E-6YNL046W_pPutative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YDL056W4.347.23E-6MBP1Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes

GO enrichment analysis for SGTC_1182
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0766.17E-9SGTC_11851319-0113 81.3 μMChemDiv (Drug-like library)46727630.0574713DNA damage response
0.0731.63E-8SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.0789474translation
0.0672.53E-7SGTC_21655727197 32.1 μMChembridge (Fragment library)13617120.103448
0.0604.03E-6SGTC_1180083-0097 135.3 μMChemDiv (Drug-like library)52073560.0714286translation
0.0588.81E-6SGTC_25992',3-dihydroxy-4,4',6'-trimethoxychalcone 100.0 μMMicrosource (Natural product library)53790710.0735294
0.0542.90E-5SGTC_1864st057645 30.4 μMTimTec (Natural product derivative library)6886660.133333amide catabolism
0.0543.36E-5SGTC_1914st062367 72.6 μMTimTec (Natural product derivative library)9782050.0882353
0.0511.02E-4SGTC_9491165-0505 146.0 μMChemDiv (Drug-like library)62442670.0625
0.0501.27E-4SGTC_2361509-0011 5.3 μMChemDiv (Drug-like library)31072800.0793651
0.0481.98E-4SGTC_28057950783 58.4 μMChembridge (Drug-like library)11789580.0789474
0.0481.98E-4SGTC_454lyso-paf C-16 6.7 μMMiscellaneous1621260.123077
0.0482.37E-4SGTC_1769st044406 64.4 μMTimTec (Natural product derivative library)6709030.0714286
0.0463.57E-4SGTC_227fluconazole 33.5 μMMiscellaneous33650.078125azole & statin
0.0463.60E-4SGTC_316k060-0003 58.6 μMChemDiv (Drug-like library)61523140.0555556
0.0463.73E-4SGTC_1108betulinic acid 729.1 nMTimTec (Natural product library)649450.0481928

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_11811222-0449220 μM1658318ChemDiv (Drug-like library)242.316223.75812
SGTC_810411-00469.97 μM0.36956512925265ChemDiv (Drug-like library)297.230645.4701
SGTC_790052-000794.09 μM0.3469392801472ChemDiv (Drug-like library)276.370664.63813
SGTC_9591222-0044107 μM0.3469396083612ChemDiv (Drug-like library)232.31814.68512
SGTC_9581222-000733 μM0.347312461ChemDiv (Drug-like library)246.344685.14112
SGTC_6641222-005414.9 μM0.3272736813991ChemDiv (Drug-like library)287.3717833.72413sphingolipid biosynthesis & PDR1
SGTC_820054-0107293.76 μM0.3207553104697ChemDiv (Drug-like library)340.412866.11514
SGTC_1121fusaric acid630.51 nM0.3061223442TimTec (Natural product library)179.215722.59213
SGTC_2961222-003974.9 μM0.35984176ChemDiv (Drug-like library)218.291524.22812
SGTC_2969909140828.7 μM0.2857144286311Chembridge (Drug-like library)254.326923.31812