1254-0324

N'-(5-bromo-2-oxoindol-3-yl)-2-(4-nitrophenyl)acetohydrazide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1183
Screen concentration 198.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5799357
SMILES C1=CC(=CC=C1CC(=O)NNC2=C3C=C(C=CC3=NC2=O)Br)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(CC(=O)NN=C2C(=O)Nc3ccc(Br)cc23)cc1
Molecular weight 403.1869
ALogP 1.5
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.54
% growth inhibition (Hom. pool) 3.48


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5799357
Download HIP data (tab-delimited text)  (excel)
Gene:CBF5(YLR175W)|FD-Score:-3.19|P-value:7.20E-4|Clearance:0||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:DIB1(YPR082C)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:DRE2(YKR071C)|FD-Score:-4.22|P-value:1.25E-5|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:ERG20(YJL167W)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.1||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:FAS1(YKL182W)|FD-Score:-4.37|P-value:6.14E-6|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:HEM3(YDL205C)|FD-Score:4.05|P-value:2.51E-5|Clearance:0.27||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:KRI1(YNL308C)|FD-Score:3.78|P-value:7.75E-5|Clearance:0.58||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MVD1(YNR043W)|FD-Score:5.79|P-value:3.43E-9|Clearance:1.74||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:PRP24(YMR268C)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.01||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RLI1(YDR091C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RSC6(YCR052W)|FD-Score:3.1|P-value:9.57E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEN1(YLR430W)|FD-Score:-3.79|P-value:7.58E-5|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SSL1(YLR005W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.12||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TEM1(YML064C)|FD-Score:-3.73|P-value:9.47E-5|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis Gene:CBF5(YLR175W)|FD-Score:-3.19|P-value:7.20E-4|Clearance:0||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:DIB1(YPR082C)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:DRE2(YKR071C)|FD-Score:-4.22|P-value:1.25E-5|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:ERG20(YJL167W)|FD-Score:3.21|P-value:6.69E-4|Clearance:0.1||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:FAS1(YKL182W)|FD-Score:-4.37|P-value:6.14E-6|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:HEM3(YDL205C)|FD-Score:4.05|P-value:2.51E-5|Clearance:0.27||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:KRI1(YNL308C)|FD-Score:3.78|P-value:7.75E-5|Clearance:0.58||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:MVD1(YNR043W)|FD-Score:5.79|P-value:3.43E-9|Clearance:1.74||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:PRP24(YMR268C)|FD-Score:3.1|P-value:9.66E-4|Clearance:0.01||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RLI1(YDR091C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RSC6(YCR052W)|FD-Score:3.1|P-value:9.57E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEN1(YLR430W)|FD-Score:-3.79|P-value:7.58E-5|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SSL1(YLR005W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.12||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TEM1(YML064C)|FD-Score:-3.73|P-value:9.47E-5|Clearance:0||SGD DESC:GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5799357
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:5.02|P-value:2.56E-7||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARO1(YDR127W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BLI1(YKL061W_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BUD30(YDL151C_d)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:COQ10(YOL008W)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COX11(YPL132W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:CSM3(YMR048W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:ECM7(YLR443W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EMP47(YFL048C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ENT5(YDR153C)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:EOS1(YNL080C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:EPS1(YIL005W)|FD-Score:4.79|P-value:8.36E-7||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERG2(YMR202W)|FD-Score:5.87|P-value:2.19E-9||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV29(YGR284C)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FAB1(YFR019W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FAU1(YER183C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FDC1(YDR539W)|FD-Score:4.83|P-value:6.99E-7||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FYV7(YLR068W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GEA1(YJR031C)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPB1(YOR371C)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HMI1(YOL095C)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HTL1(YCR020W-B)|FD-Score:5.22|P-value:8.90E-8||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:HTZ1(YOL012C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IRC3(YDR332W)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:KNS1(YLL019C)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:KTR7(YIL085C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MAL31(YBR298C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MAM1(YER106W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MET13(YGL125W)|FD-Score:-3.27|P-value:5.33E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MHF2(YDL160C-A)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MHT1(YLL062C)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:MIC23(YBL107C_p)|FD-Score:-4.27|P-value:9.90E-6||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MON2(YNL297C)|FD-Score:-6.01|P-value:9.16E-10||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRPL31(YKL138C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL6(YHR147C)|FD-Score:4.35|P-value:6.90E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTF2(YDL044C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:OCT1(YKL134C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPY1(YBR129C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PET54(YGR222W)|FD-Score:4.89|P-value:5.07E-7||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:POP2(YNR052C)|FD-Score:5.31|P-value:5.43E-8||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM4(YPL156C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:RBS1(YDL189W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RIM1(YCR028C-A)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIM15(YFL033C)|FD-Score:4.77|P-value:9.04E-7||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RPL4A(YBR031W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RXT3(YDL076C)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAP155(YFR040W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SAP4(YGL229C)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SBH1(YER087C-B)|FD-Score:4.93|P-value:4.06E-7||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SIC1(YLR079W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SNF5(YBR289W)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SUS1(YBR111W-A)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWC3(YAL011W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SYF2(YGR129W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRP3(YKL211C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP13(YBL067C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VPS60(YDR486C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:YBR090C(YBR090C_p)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YCR102C(YCR102C_p)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YJL169W(YJL169W_d)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKR011C(YKR011C_p)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR202C(YLR202C_d)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Putative protein of unknown function Gene:YMR1(YJR110W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YMR102C(YMR102C_p)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNR048W(YNR048W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOR309C(YOR309C_d)|FD-Score:5.18|P-value:1.10E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPL102C(YPL102C_d)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR012W(YPR012W_d)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YRR1(YOR162C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ADE8(YDR408C)|FD-Score:5.02|P-value:2.56E-7||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARO1(YDR127W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BLI1(YKL061W_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BUD30(YDL151C_d)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:COQ10(YOL008W)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COX11(YPL132W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:CSM3(YMR048W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:ECM7(YLR443W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:EMP47(YFL048C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ENT5(YDR153C)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:EOS1(YNL080C)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:EPS1(YIL005W)|FD-Score:4.79|P-value:8.36E-7||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERG2(YMR202W)|FD-Score:5.87|P-value:2.19E-9||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV29(YGR284C)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FAB1(YFR019W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FAU1(YER183C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FDC1(YDR539W)|FD-Score:4.83|P-value:6.99E-7||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FYV7(YLR068W)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GEA1(YJR031C)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPB1(YOR371C)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HMI1(YOL095C)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HTL1(YCR020W-B)|FD-Score:5.22|P-value:8.90E-8||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:HTZ1(YOL012C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IRC3(YDR332W)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:KNS1(YLL019C)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:KTR7(YIL085C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MAL31(YBR298C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MAM1(YER106W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MET13(YGL125W)|FD-Score:-3.27|P-value:5.33E-4||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MHF2(YDL160C-A)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MHT1(YLL062C)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:MIC23(YBL107C_p)|FD-Score:-4.27|P-value:9.90E-6||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MON2(YNL297C)|FD-Score:-6.01|P-value:9.16E-10||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MRPL31(YKL138C)|FD-Score:4.61|P-value:2.00E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL6(YHR147C)|FD-Score:4.35|P-value:6.90E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS8(YMR158W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MTF2(YDL044C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:OCT1(YKL134C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPY1(YBR129C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PET54(YGR222W)|FD-Score:4.89|P-value:5.07E-7||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:POP2(YNR052C)|FD-Score:5.31|P-value:5.43E-8||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM4(YPL156C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:RBS1(YDL189W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RIM1(YCR028C-A)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIM15(YFL033C)|FD-Score:4.77|P-value:9.04E-7||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RPL4A(YBR031W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RXT3(YDL076C)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAP155(YFR040W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SAP4(YGL229C)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SBH1(YER087C-B)|FD-Score:4.93|P-value:4.06E-7||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SIC1(YLR079W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SNF5(YBR289W)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SUS1(YBR111W-A)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWC3(YAL011W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SYF2(YGR129W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TRP3(YKL211C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP13(YBL067C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VPS60(YDR486C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:YBR090C(YBR090C_p)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YCR102C(YCR102C_p)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YJL169W(YJL169W_d)|FD-Score:3.84|P-value:6.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YKR011C(YKR011C_p)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YLR202C(YLR202C_d)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Putative protein of unknown function Gene:YMR1(YJR110W)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YMR102C(YMR102C_p)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YNR048W(YNR048W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YOR309C(YOR309C_d)|FD-Score:5.18|P-value:1.10E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPL102C(YPL102C_d)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR012W(YPR012W_d)|FD-Score:3.89|P-value:4.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YRR1(YOR162C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNR043W5.793.43E-91.74MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YDL205C4.052.51E-50.27HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YNL308C3.787.75E-50.57KRI1Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
YJL167W3.216.69E-40.10ERG20Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
YCR052W3.109.57E-40.00RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YMR268C3.109.66E-40.01PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YLR005W3.099.99E-40.12SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YOL069W2.970.001480.01NUF2Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YML127W2.960.001510.01RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YGL040C2.960.001560.07HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YLR051C2.880.001980.11FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YLR076C_d2.770.002770.10YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YDR118W2.680.003720.05APC4Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
YDR002W2.630.004260.03YRB1Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
YMR290C2.600.004600.00HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W5.872.19E-9ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YNR052C5.315.43E-8POP2RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
YCR020W-B5.228.90E-8HTL1Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YOR309C_d5.181.10E-7YOR309C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
YDR408C5.022.56E-7ADE8Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
YER087C-B4.934.06E-7SBH1Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p
YGR222W4.895.07E-7PET54Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing
YDR539W4.836.99E-7FDC1Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm
YIL005W4.798.36E-7EPS1ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family
YFL033C4.779.04E-7RIM15Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
YLL019C4.691.37E-6KNS1Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
YGR129W4.691.38E-6SYF2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest
YKL138C4.612.00E-6MRPL31Mitochondrial ribosomal protein of the large subunit
YHR147C4.356.90E-6MRPL6Mitochondrial ribosomal protein of the large subunit
YLR079W4.221.24E-5SIC1Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1

GO enrichment analysis for SGTC_1183
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1111.48E-17SGTC_11750741-0056 27.6 μMChemDiv (Drug-like library)7659270.108434
0.0992.55E-14SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.08ERG2
0.0905.40E-12SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0461538ERG2
0.0881.49E-11SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0561798ERG2
0.0872.06E-11SGTC_12470448-0043 5.8 μMChemDiv (Drug-like library)30890130.05ERG2
0.0872.64E-11SGTC_31779107506 49.5 μMChembridge (Drug-like library)88971940.120482ERG2
0.0857.34E-11SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.045977ERG2
0.0831.74E-10SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.125ERG2
0.0822.36E-10SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.149425ERG2
0.0814.59E-10SGTC_1028k015-0029 4.8 μMChemDiv (Drug-like library)45883090.25
0.0814.74E-10SGTC_635k038-0028 24.7 μMChemDiv (Drug-like library)40351020.0555556ubiquinone biosynthesis & proteasome
0.0815.67E-10SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.0549451ERG2
0.0815.72E-10SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.144737ERG2
0.0772.78E-9SGTC_7420kpi-0133 140.0 μMChemDiv (Drug-like library)27288520.118421ERG2
0.0773.24E-9SGTC_1167k781-0610 148.0 μMChemDiv (Drug-like library)45251690.0864198RNA processing & uracil transport

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_740k014-0057261 μM0.5217394012779ChemDiv (Drug-like library)510.253883.23427
SGTC_196k015-002318.11 μM0.3968255211998ChemDiv (Drug-like library)361.146963.10204PDR1
SGTC_20545233173129 μM0.3773586889Chembridge (Fragment library)226.026861.41812mitochondrial response to ROS
SGTC_9252064-127259.6 μM0.3768126815817ChemDiv (Drug-like library)333.726481.44825
SGTC_850868-0259160.06 μM0.37288183173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_289k017-0060526 μM0.3384625512909ChemDiv (Drug-like library)380.21651.56725
SGTC_990109-0120622.8 μM0.333333759414ChemDiv (Drug-like library)321.126163.25713
SGTC_1027k015-00258.67 μM0.3188413598955ChemDiv (Drug-like library)361.146963.10204
SGTC_1340269-00186.92 μM0.3076924378915ChemDiv (Drug-like library)411.121283.04617
SGTC_10543429-0080167 μM0.2837841162877ChemDiv (Drug-like library)399.281063.20113